全文获取类型
收费全文 | 7782篇 |
免费 | 693篇 |
国内免费 | 450篇 |
出版年
2024年 | 15篇 |
2023年 | 484篇 |
2022年 | 281篇 |
2021年 | 331篇 |
2020年 | 387篇 |
2019年 | 305篇 |
2018年 | 341篇 |
2017年 | 287篇 |
2016年 | 356篇 |
2015年 | 403篇 |
2014年 | 399篇 |
2013年 | 573篇 |
2012年 | 395篇 |
2011年 | 374篇 |
2010年 | 333篇 |
2009年 | 431篇 |
2008年 | 295篇 |
2007年 | 314篇 |
2006年 | 315篇 |
2005年 | 256篇 |
2004年 | 233篇 |
2003年 | 187篇 |
2002年 | 191篇 |
2001年 | 154篇 |
2000年 | 148篇 |
1999年 | 157篇 |
1998年 | 125篇 |
1997年 | 112篇 |
1996年 | 104篇 |
1995年 | 80篇 |
1994年 | 61篇 |
1993年 | 70篇 |
1992年 | 58篇 |
1991年 | 60篇 |
1990年 | 32篇 |
1989年 | 41篇 |
1988年 | 33篇 |
1987年 | 32篇 |
1986年 | 22篇 |
1985年 | 24篇 |
1984年 | 31篇 |
1983年 | 10篇 |
1982年 | 18篇 |
1981年 | 15篇 |
1980年 | 11篇 |
1979年 | 16篇 |
1978年 | 8篇 |
1977年 | 4篇 |
1976年 | 5篇 |
1973年 | 2篇 |
排序方式: 共有8925条查询结果,搜索用时 546 毫秒
1.
The influence of fruit ingestion by the bat, Sturnira lilium, on germination of the seeds of the tree Solanum riparium was studied in a secondary rain forest in northwestern Argentina. Bat frequencies in disturbed areas were analyzed by mist net captures. Germination rates were determined for seeds collected from trees and bat feces. S. lilium was the most abundant fruit bat in the study area. Fruit digestion and the passage of seeds through the intestine did not significantly affect germination in S. riparium. In this case the fruit bats, therefore, probably provide only seed dispersal. 相似文献
2.
《Critical reviews in biochemistry and molecular biology》2013,48(5):409-435
Telomere DNA-binding proteins protect the ends of chromosomes in eukaryotes. A subset of these proteins are constructed with one or more OB folds and bind with G+T-rich single-stranded DNA found at the extreme termini. The resulting DNA-OB protein complex interacts with other telomere components to coordinate critical telomere functions of DNA protection and DNA synthesis. While the first crystal and NMR structures readily explained protection of telomere ends, the picture of how single-stranded DNA becomes available to serve as primer and template for synthesis of new telomere DNA is only recently coming into focus. New structures of telomere OB fold proteins alongside insights from genetic and biochemical experiments have made significant contributions towards understanding how protein-binding OB proteins collaborate with DNA-binding OB proteins to recruit telomerase and DNA polymerase for telomere homeostasis. This review surveys telomere OB protein structures alongside highly comparable structures derived from replication protein A (RPA) components, with the goal of providing a molecular context for understanding telomere OB protein evolution and mechanism of action in protection and synthesis of telomere DNA. 相似文献
3.
《Molecular & cellular proteomics : MCP》2020,19(7):1161-1178
- Download : Download high-res image (250KB)
- Download : Download full-size image
- •XL-MS reveals new PPIs in yeast mitochondria under glycerol and glucose condition.
- •Significant but limited results from quantitative XL-MS experiments.
- •Ndi1 participates in a CIII2CIV2 respiratory supercomplex.
- •Min8 promotes assembly of Cox12 into an intermediate complex IV.
4.
Spencer C. H. Barrett 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2010,365(1537):99-109
Flowering plants display spectacular floral diversity and a bewildering array of reproductive adaptations that promote mating, particularly outbreeding. A striking feature of this diversity is that related species often differ in pollination and mating systems, and intraspecific variation in sexual traits is not unusual, especially among herbaceous plants. This variation provides opportunities for evolutionary biologists to link micro-evolutionary processes to the macro-evolutionary patterns that are evident within lineages. Here, I provide some personal reflections on recent progress in our understanding of the ecology and evolution of plant reproductive diversity. I begin with a brief historical sketch of the major developments in this field and then focus on three of the most significant evolutionary transitions in the reproductive biology of flowering plants: the pathway from outcrossing to predominant self-fertilization, the origin of separate sexes (females and males) from hermaphroditism and the shift from animal pollination to wind pollination. For each evolutionary transition, I consider what we have discovered and some of the problems that still remain unsolved. I conclude by discussing how new approaches might influence future research in plant reproductive biology. 相似文献
5.
Benjamin C. Blum Weiwei Lin Matthew L. Lawton Qian Liu Julian Kwan Isabella Turcinovic Ryan Hekman Pingzhao Hu Andrew Emili 《Molecular & cellular proteomics : MCP》2022,21(1):100189
Metabolism is recognized as an important driver of cancer progression and other complex diseases, but global metabolite profiling remains a challenge. Protein expression profiling is often a poor proxy since existing pathway enrichment models provide an incomplete mapping between the proteome and metabolism. To overcome these gaps, we introduce multiomic metabolic enrichment network analysis (MOMENTA), an integrative multiomic data analysis framework for more accurately deducing metabolic pathway changes from proteomics data alone in a gene set analysis context by leveraging protein interaction networks to extend annotated metabolic models. We apply MOMENTA to proteomic data from diverse cancer cell lines and human tumors to demonstrate its utility at revealing variation in metabolic pathway activity across cancer types, which we verify using independent metabolomics measurements. The novel metabolic networks we uncover in breast cancer and other tumors are linked to clinical outcomes, underscoring the pathophysiological relevance of the findings. 相似文献
6.
7.
《Molecular & cellular proteomics : MCP》2020,19(1):142-154
- Download : Download high-res image (133KB)
- Download : Download full-size image
- •Flow cytometry analysis is used to isolate ASC speck(+) NPC cells.
- •Proteome analysis of ASC speck(+) NPC cells reveals enriched mitochondrial OxPhos proteins.
- •OxPhos proteins mediate NLRP3 inflammasome activation through mtROS.
- •OxPhos proteins, NDUFB8 and ATP5B are correlated with NPC local recurrence.
8.
W. Kelley Thomas J. T. Vida Linda M. Frisse Manuel Mundo James G. Baldwin 《Journal of nematology》1997,29(3):250-254
To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses. 相似文献
9.
Lucas Henriques Viscardi Danilo Oliveira Imparato Maria Ctira Bortolini Rodrigo Juliani Siqueira Dalmolin 《Molecular biology and evolution》2021,38(3):735
The origin of nervous systems is a main theme in biology and its mechanisms are largely underlied by synaptic neurotransmission. One problem to explain synapse establishment is that synaptic orthologs are present in multiple aneural organisms. We questioned how the interactions among these elements evolved and to what extent it relates to our understanding of the nervous systems complexity. We identified the human neurotransmission gene network based on genes present in GABAergic, glutamatergic, serotonergic, dopaminergic, and cholinergic systems. The network comprises 321 human genes, 83 of which act exclusively in the nervous system. We reconstructed the evolutionary scenario of synapse emergence by looking for synaptic orthologs in 476 eukaryotes. The Human–Cnidaria common ancestor displayed a massive emergence of neuroexclusive genes, mainly ionotropic receptors, which might have been crucial to the evolution of synapses. Very few synaptic genes had their origin after the Human–Cnidaria common ancestor. We also identified a higher abundance of synaptic proteins in vertebrates, which suggests an increase in the synaptic network complexity of those organisms. 相似文献
10.