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李月灵;金则新;管铭;左威 《植物研究》2013,33(6):684-689
为探讨铜(Cu)胁迫条件下土壤微生物对海州香薷(Elsholtzia splendens)光合生理和叶绿素荧光参数的影响,实验设置添加Cu(Cu胁迫)、接种土壤微生物、添加Cu与接种土壤微生物等3个处理,以不添加Cu与不接种土壤微生物为对照(CK)。结果表明:接种土壤微生物处理的植株相对叶绿素含量、净光合速率(Pn)、水分利用效率(WUE)均显著高于CK;且对初始荧光(Fo)和最大光化学效率(Fv/Fm)均有显著性影响。与CK相比,添加Cu降低了海州香薷的Pn和气孔导度(Gs),但胞间CO2浓度(Ci)的变化与Pn相反,表明其对光合作用的影响主要是非气孔限制因素。添加Cu的植株相对叶绿素含量显著下降,但Cu胁迫下接种土壤微生物提高了植株相对叶绿素含量,差异显著。在Cu胁迫条件下,接种土壤微生物的植株具有较高的Fv/Fm及较低的Fo,显著提高了海州香薷的WUE、Pn、Gs。说明接种土壤微生物可通过提高相对叶绿素含量、改善叶绿素荧光和光合作用来减轻Cu胁迫对海州香薷植株造成的伤害,从而提高海州香薷耐受Cu胁迫的能力。 相似文献
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A farnesyl diphosphate synthase gene (FPPS2), which contains 11 introns and 12 exons, was isolated from the apple cultivar “White Winter Pearmain”. When it was compared to our previously reported FPPS1, its each intron size was different, its each exon size was the same as that of FPPS1 gene, 30 nucleotide differences were found in its coding sequence. Based on these nucleotide differences, specific primers were designed to perform expression analysis; the results showed that it expressed in both fruit and leaf, its expression level was obviously lower than that of FPPS1 gene in fruit which was stored at 4 °C for 5 weeks. This is the first report concerning two FPPS genes and their expression comparison in apples. 相似文献
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Clauber Henrique Souza Costa Amanda Ruslana Santana Oliveira Alberto M. dos Santos Kauê Santana da Costa Anderson Henrique Lima e Lima Cláudio N. Alves 《Journal of biomolecular structure & dynamics》2013,31(16):4374-4383
AbstractThe enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is mainly involved in the regulation of cholesterol biosynthesis. HMGR catalyses the reduction of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) to mevalonate at the expense of two NADPH molecules in a two-step reversible reaction. In the present study, we constructed a model of human HMGR (hHMGR) to explore the conformational changes of HMGR in complex with HMG-CoA and NADPH. In addition, we analysed the complete sequence of the Flap domain using molecular dynamics (MD) simulations and principal component analysis (PCA). The simulations revealed that the Flap domain plays an important role in catalytic site activation and substrate binding. The apo form of hHMGR remained in an open state, while a substrate-induced closure of the Flap domain was observed for holo hHMGR. Our study also demonstrated that the phosphorylation of Ser872 induces significant conformational changes in the Flap domain that lead to a complete closure of the active site, suggesting three principal conformations for the first stage of hHMGR catalysis. Our results were consistent with previous proposed models for the catalytic mechanism of hHMGR.Communicated by Ramaswamy H. Sarma 相似文献
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