电针镇痛时人脑脊液中β-内啡肽样免疫活性物质的含量与痛阈的关系

用β-内啡肽放射免疫分析法测定10例无疼痛主诉的患者电针前后侧脑室脑脊液中B-内啡肽样免疫活性物质(B-EPIS)的含量,其中6例用弹簧棒测定了痛阈和耐痛阈。结果表明,每 ml 脑脊液中β-EPIS 的含量比针前增加126.7 fmol/ml(P<0.02),病人的痛阈比针前升高29.5%,耐痛阈升高28.1%(P<0.05)。β-EPIS 含量的增加量与痛阈、耐痛阈的升高值呈直线相关,r_1=0.776,r_2=0.741(P<0.05)。表明电针能促使脑内释放β-内啡肽,释放增加的β-内啡肽参与镇痛作用,这可能是电针镇痛机制的重要环节之一。     

Spatial analysis of hepatocellular carcinoma and socioeconomic status in China from a Population-based Cancer Registry

Background: Little is known about geographic variations in liver cancer at high incident regions. We aimed to identify spatial variation of hepatocellular carcinoma (HCC) at a high-risk area in China and determine its association with socioeconomic status (SES). Methods: Based on 2299 liver cancer cases diagnosed in Haimen from 2003 to 2006, we calculated age–sex standardized incidence ratios (SIRs) and used two spatial scan statistics to determine the geographic variations in HCC. Bayesian hierarchical model was used to explore the association between HCC incidence and SES. Results: Age and sex SIRs for HCC varied from 0.54 to 1.97 for 24 townships. The eastern region of Haimen was identified to have a significantly increased risk of HCC. Fitting of a Bayesian hierarchical model linking per-capita fiscal revenue with SIRs of HCC indicated that the area with a lower revenue had a significantly higher incidence of HCC [β_log(revenue) = ?0.179, posterior 95% Bayesian credible interval (CI) = (?0.326, ?0.04)]. Conclusions: This study demonstrated substantial geographic variation in the incidence of HCC within a high-risk region, which was associated with SES. HCC control and intervention should focus on disadvantaged areas to reduce the HCC disparities.     

The effects of dispersal limitation and topographic heterogeneity on beta diversity and phylobetadiversity in a subtropical forest

We assessed the effects of topographic heterogeneity and stem density on species composition between grains of different sizes (20 × 20, 50 × 50, and 100 × 100 m), based on partial Mantel tests. Similarity in species composition was measured by the abundance-based Jaccard index (C_J) and by an index that incorporates phylogenetic information into C_J (pC_J). Plants were divided into five groups, arbor, subarbor, and shrub according to life form and two other groups: species that produce dry fruits (PDF) and that produce fleshy fruits (PFF). C_J and pC_J between any two grains at each grain size were calculated separately for these groups and for all species combined. In order to examine what influences C_J and pC_J, we analyzed their correlations with topographic heterogeneity variables and two dispersal limitation-related variables (stem and topographic resistance). Our data indicate that at all three grain sizes, C_J and pC_J decrease with increasing distance for all plant groups. Dispersal limitation and topographic heterogeneity were both important at 20 × 20 and 50 × 50 m grain sizes for C_J and pC_J of all plant groups; and at 100 × 100 m grain size, topographic heterogeneity dominates over dispersal limitation for some plant groups. C_J and pC_J of PDFs are less negatively correlated with stem resistance than those of PFFs. We conclude that both beta diversity and phylobetadiversity are dependent on plant groups and grain sizes.     

我国广东路氏锥虫的研究Ⅰ.种的特性及其在实验寄主中的繁殖

本文对发现于广东的鼠锥虫进行了寄主特异性、形态学、实验寄主体内的感染过程、厦虫氨基酸组分等多方面的研究,并将其定为路氏锥虫在中国广东分布的一个不同地域株。本文研究了它在实验寄主体内繁殖消长的规律,整个发育过程的各期形态及成熟期的超微结构,并测出其16种氨基酸的存在和含量。     

Classical Raman spectroscopic studies of NADH and NAD+ bound to liver alcohol dehydrogenase by difference techniques

We report the Raman spectra of reduced and oxidized nicotinamide adenine dinucleotide (NADH and NAD+, respectively) and adenosine 5'-diphosphate ribose (ADPR) when bound to the coenzyme site of liver alcohol dehydrogenase (LADH). The bound NADH spectrum is calculated by taking the classical Raman difference spectrum of the binary complex, LADH/NADH, with that of LADH. We have investigated how the bound NADH spectrum is affected when the ternary complexes with inhibitors are formed with dimethyl sulfoxide (Me2SO) or isobutyramide (IBA), i.e., LADH/NADH/Me2SO or LADH/NADH/IBA. Similarly, the difference spectra of LADH/NAD+/pyrazole or LADH/ADPR with LADH are calculated. The magnitude of these difference spectra is on the order of a few percent of the protein Raman spectrum. We report and discuss the experimental configuration and control procedures we use in reliably calculating such small difference signals. These sensitive difference techniques could be applied to a large number of problems where the classical Raman spectrum of a "small" molecule, like adenine, bound to the active site of a protein is of interest. The spectrum of bound ADPR allows an assignment of the bands of the bound NADH and NAD+ spectra to normal coordinates located primarily on either the nicotinamide or the adenine moiety. By comparing the spectra of the bound coenzymes with model compound data and through the use of deuterated compounds, we confirm and characterize how the adenine moiety is involved in coenzyme binding and discuss the validity of the suggestion that the adenine ring is protonated upon binding. The nicotinamide moiety of NADH shows significant molecular changes upon binding.(ABSTRACT TRUNCATED AT 250 WORDS)     

激光照射胚泡对家兔和小鼠的抗早孕研究

本文在家兔、小鼠上探索了激光的抗旱孕效应,观察到血卟啉衍生物(HPD)和激光的光化效应能使早期胚泡完全坏死和吸收,无流血和晚期流产现象,通过PNQ_3荧光分光光度计测定HPD在胚胎组织中的荧光谱,证实HPD对胚胎组织有亲和力,其对滋养叶细胞比对宫壁组织的亲和力大4倍,借以解释激光光化效应抗早孕的可能机理。     

大齿大尾溞一新亚种(鳃足纲:双甲目)

本文绘图描述了大齿大尾骚的一新亚种,定名为长毛大尾溞Leydigia macrondonta longiseta subsp.nov.新亚种与其它亚种的主要不同是:后腹部每簇侧刚毛中的3—4根刚毛自前至后趋长,并且都比较长。