共查询到20条相似文献,搜索用时 46 毫秒
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生物工程综合性实验课程以企业人才需求为导向,解决实际生产过程中的复杂工程问题为教学目标,利用两步酶转化法制备L-天冬氨酸和L-丙氨酸的工艺路线,结合生物工程专业生产工艺管理的特点,借鉴生产企业现场管理经验,实施四班三运转的实验运行方式。成绩考核加入交接班总结评价与团队协作评价,该课程设置了包含多门专业核心课程原理、方法与实验技术和企业生产管理模式的新型生物工程综合性实验教学内容,并通过教学实践,持续改进,形成完整的实验教学过程与考核机制。生物工程综合性实验课程取得了良好的教学效果,促进了生物工程专业实验教学的发展。 相似文献
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分析生物工程专业人才需求的现状与发展前景,构建了以社会需求为导向,以实践课程体系为载体,以能力培养为主线,以学生科技创新活动和教师科研为驱动,项目研究与能力培养相结合的创新实践教学模式.实践表明,创新实践教学模式极大提高了学生的学习兴趣和教师的工作热情. 相似文献
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Birefringence of flow of preparations of myosin 总被引:4,自引:0,他引:4
BINKLEY F 《The Journal of biological chemistry》1948,175(1):385-390
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A sensitive method for the detection of small quantities of hydrophobic antioxidant free radical scavengers such as butylatedhydroxytoluene (BHT) and butylatedhydroxyanisole (BHA) in aqueous samples is described. The procedure involves extraction of the hydrophobic free radical scavenger into an organic solvent phase, followed by the subsequent reaction of an aliquot of this extract with the stable cation radical tris(p-bromophenyl)amminium hexachloroantimonate (TBACA). In experiments with BHT and BHA, the loss of TBACA absorbance at 730 nm was found to be linearly proportional to the amount of antioxidant added, with quantities of BHT as small as 200 pmol being easily detectable. In aqueous suspensions of dimyristoylphosphatidylcholine vesicles, assays of the aqueous BHT concentration showed that BHT partitioned strongly into the membrane phase, achieving very high BHT/phospholipid ratios. For a given concentration of BHT, partitioning into the membrane phase was greater in large, multilamellar liposomes than in either small, single-walled vesicles or in purified rat brain synaptic vesicle membranes. Direct assay of BHT and BHA in phospholipid membranes, however, was complicated by a nonspecific interaction between TBACA and the phospholipid. 相似文献
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Henry B. F. Dixon 《The Biochemical journal》1973,131(1):149-154
A simple case is considered in which the rate of a two-step reaction depends on pH because the intermediate formed in the first step has to gain (or lose) a proton before it can react in the second step, and in which the rate-determining step therefore changes with pH. The curves of reaction rate against pH are shown to be symmetrical, and the sharpest peak possible has a width at half its height of 1.53pH units, i.e. of 2log(3+2 radical2). Any particular curve for this situation proves to be identical with a curve that could be generated for the pH-dependence of a single-step reaction in which the rate is proportional to the concentration of a particular ionic form of a reactant. Curves for the latter situation, however, can have forms impossible for the former case in which the rate-determining step changes, but only if the protonations that activate and deactivate the reactant are co-operative. The peak can then become even sharper, and its width at half its height can fall to 1.14pH units, i.e. to 2log(2+ radical3). 相似文献
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