人附红细胞体感染FMMU白化豚鼠的研究

用附红细胞体分别感染FMMU白化豚鼠和普通花色豚鼠,同时测定两组豚鼠的红细胞免疫功能,探讨FMMU白化豚鼠的免疫学特性与病原体敏感性之间的关系。结果表明,FMMU白化豚鼠对人附红细胞体比普通花色豚鼠敏感。封闭群FMMU白化豚鼠有独特的免疫学特性,红细胞免疫功能低于普通花色豚鼠,对病原体敏感性高于普通花色豚鼠,更适于建立感染性疾病动物模型。     

急性高原病患者血清和支气管肺泡灌洗液炎性反应物的改变

目的：探讨炎症反应在急性高原病发病中的作用。方法：采用支气管-肺泡灌洗法及单向免疫扩散测量法。结果：HAPE患者外周血中C-反性蛋白及免疫球蛋白(IgG、IgA、IgM)含量均显著高于HAAR组及对照组,补体C3、C4与对照组差别无显著性;HAPE患者肺泡灌洗液中含有大量蛋白质、红细胞及白细胞,含有C-反应蛋白,大量的免疫球蛋白(IgG、IgA、IgM)和补体C3-C4。结论：在HAPE发病过程中,除了肺动脉高压是其发病的重要因素外,炎症损伤亦起一定作用;而HAAR发病过程中,未见炎性反应发生。     

百草枯中毒患者血清补体及免疫球蛋白含量的变化及意义

目的:比较分析百草枯中毒患者血清补体水平及免疫球蛋白含量的改变及临床意义.方法:免疫球透射比浊法检测32例百草枯中毒患者(中毒组)与40例健康者(对照组)血清中补体C3,C5的水平及血清IgG、IgM、IgA含量.结果:百草枯中毒组患者血清IgM、C3、C5含量与对照组相比增高,且具有显著性差异(P<0.05或P<0.01),而IgG、IgA含量与对照组相比无明显变(P>0.05).结论:血清补体C3,C5水平与百草枯中毒患者的发病机制密切相关,抑制补体系统的激活,可能是治疗百草枯中毒患者的一种途径.     

反复呼吸道感染患儿血清微量元素及体液免疫水平测定及临床意义

目的:探讨反复呼吸道感染患儿血清微量元素及体液免疫水平测定及其临床意义。方法:选取2016年1月至2017年1月在我院接受治疗的反复呼吸道感染患儿64例作为观察组,另外选取同期来我院体检的健康儿童60例作为对照组,比较两组儿童血清微量元素钙(Ca)、铁(Fe)、铜(Cu)、锌(Zn)、镁(Mg)等的水平、体液免疫因子免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)水平及血清补体C3、C4、C5水平,并分析其相关性。结果:观察组患儿血清Ca、Fe、Zn水平显著低于对照组儿童(P0.05),两组儿童血清Cu、Mg水平比较差异无统计学意义(P0.05)。观察组患儿血清IgA、IgM、IgG水平低于对照组儿童(P0.05)。两组儿童血清补体C3、C4、C5水平比较差异无统计学意义(P0.05)。经Pearson相关性分析可得:反复呼吸道感染患儿血清Ca、Fe、Zn与血清IgA、IgM、IgG水平呈正相关(P0.05)。结论:反复呼吸道感染患儿存在血清Ca、Fe、Zn微量元素缺乏及血清IgA、IgM、IgG水平降低现象,且它们之间具有正相关关系,可能共同促进反复呼吸道感染的发生。     

水痘-带状疱疹病毒对系统性红斑狼疮患者体液免疫功能的影响

目的:观察水痘患者、系统性红斑狼疮(systemic lupus erythematosus,SLE)患者和系统性红斑狼疮合并水痘患者体液免疫指标的变化,探讨水痘-带状疱疹病毒对系统性红斑狼疮患者体液免疫功能的影响。方法:选择在我院诊断治疗的水痘患者、系统性红斑狼疮患者和系统性红斑狼疮合并水痘患者共66例,同时设21例正常对照组;利用血液细胞分析仪检测各组血液中白细胞计数(WBC)、血小板计数(PLT)以及血红蛋白含量(HGB);采用免疫比浊法检测各组血清中免疫球蛋白G(IgG)、免疫球蛋白A(IgA)和免疫球蛋白M(IgM)以及补体C3、C4的水平。结果:与正常对照组比较,水痘组、SLE组以及SLE合并水痘组WBC、PLT和HGB含量下降,其中SLE组和SLE合并水痘组WBC、PLT降低,差异有统计学意义(P<0.05或P<0.01);水痘组血清中IgG、IgA和IgM含量下降,SLE组和SLE合并水痘组血清中IgG、IgA和IgM含量上升,其中水痘组血清中IgA含量减少有统计学意义(P<0.05),SLE组和SLE合并水痘组血清中IgG、IgA含量增加有统计学意义(P<0.05或P<0.01);水痘组血清中补体C3、C4含量增加,SLE组和SLE合并水痘组血清中补体C3、C4含量减少,其中SLE合并水痘组减少且差异有统计学意义(P<0.05)。与SLE组比较,SLE合并水痘组WBC明显增加(P<0.05),血清中IgG、IgA和补体C3、C4降低且差异有统计学意义(P<0.05)。结论:水痘-带状疱疹病毒可引起系统性红斑狼疮患者免疫系统相关指标的改变并对其产生影响。     

与豚鼠红细胞形成自然花环的狗淋巴细胞类别的探讨

本文研究了豚鼠E花环形成狗淋巴细胞(E-RFC)的类别。狗淋巴细胞于37℃孵育后,可提高E花环形成率。E-RFC与总淋巴细胞的SIg阳性率相近。E-EAC(豚鼠红细胞—抗体和补体包被的绵羊红细胞)混合花环实验表明一部分狗淋巴细胞同时具有E和补体二种受体,总淋巴细胞与补体受体淋巴细胞的E花环阳性率无显著差异。这些结果说明E受体不仅表现于一部分T细胞上,而且见于某些B细胞上。因此,不宜用E花环试验作为检测狗T细胞的方法。     

气温骤降前后慢性阻塞性肺疾病患者免疫情况的变化

目的:探讨COPD患者免疫功能在外界温度变化时的变化.方法:T淋巴细胞亚群测定采用单克隆抗体免疫组化荧光染色法.血清免疫球蛋白(IgG、IgA、IgM)用琼脂单向扩散法.结果:COPD患者体温骤降后CD3、CD4、CD4/CD8明显降低,CD8明显升高,IgG、IgA、IgM降低,与对照组相比有显著性差异(P＜0.01).体温下降前与体温下降后比较差异无统计学意义(P＞0.05).结论:体温骤降致COPD患者细胞免疫和体液免疫功能失衡,体温骤降后较体温骤降前体液免疫及细胞免疫功能进一步降低.     

白癜风患儿抗核抗体、免疫球蛋白及补体检测的临床分析

目的:探讨白癜风患儿中抗核抗体(ANA)、免疫球蛋白(IgG、IgA、IgM)以及补体(C3、C4)的表达及临床意义。方法:收集2014年10月至2016年5月我院收治的30例白癜风患儿为病例组,并于同期随机选取30例健康体检儿童为对照组,患儿的血清ANA、12种自身抗体谱分别采用间接免疫荧光法及免疫印迹法进行检测,IgG、IgA、IgM,C3、C4水平采用免疫透射比浊法进行检测。结果:病例组中ANA阳性率为13.33%(4/30),与对照组的3.33%(1/30)比较,差异无统计学意义(P0.05)。12种自身抗体谱中,分别有1例(3.33%)患儿dsDNA、SmDNA、SS-A/Ro60KD、SS-B/La、CENP-B阳性,与对照组比较,差异均无统计学意义(P0.05)。病例组患儿血清IgG、IgA、C4水平低于对照组,差异有统计学意义(P0.05)。ANA阳性患儿血清IgG、IgA、IgM水平高于ANA阴性患儿,而补体C3、C4水平低于ANA阴性患儿,差异有统计学意义(P0.05)。结论:白癜风患儿ANA阳性表达与健康儿童无明显区别,体液免疫功能有明显异常,临床有重要的参考价值。     

过敏性紫癜合并肺炎支原体感染患儿呼吸道菌群多样性与补体C3、C4及免疫指标的相关性

目的研究儿童过敏性紫癜(HSP)合并肺炎支原体(MP)感染患儿呼吸道菌群多样性与补体C3、C4及免疫指标的相关性。方法选择2017年3月至2020年3月于我院接受治疗的HSP患儿96例,其中48例合并MP感染的患儿设为合并MP组,48例未合并MP感染的患儿设为HSP组。另外选择同期我院健康体检儿童45例为对照组。比较3组对象呼吸道菌群多样性,血清免疫球蛋白IgG、IgA、IgM、IgE及补体C3、C4水平,并分析呼吸道菌群多样性与免疫指标间的相关性。结果合并MP组患儿呼吸道菌群Simpson指数显著高于对照组,Shannon指数显著小于对照组(均P<0.05)。HSP组患儿呼吸道菌群Simpson指数及Shannon指数与对照比较差异无统计学意义(均P>0.05)。合并MP组患儿血清IgA水平较对照组显著上升,IgM水平显著下降;HSP组患儿血清IgA、IgE水平较对照组显著上升;合并MP组患儿血清IgE、IgM水平显著低于HSP组(均P<0.05);而3组对象血清IgG水平比较差异无统计学意义(均P>0.05)。合并MP组和HSP组患儿血清补体C3水平显著低于对照组;合并MP组患儿血清补体C4水平显著低于对照组;合并MP组患儿血清补体C3、C4水平均显著低于HSP组(均P<0.05)。IgM、IgE及补体、C3、C4水平与Simpson指数呈负相关,与Shannon指数呈正相关(均P<0.05)。结论HSP合并MP感染患儿呼吸道菌群多样性与补体C3、C4及IgM、IgE水平具有显著相关性。     

鸡红细胞免疫吸附性能与白细胞数量关系

应用红细胞C3b受体花环(G3b Receptor Rosette,G3bRR)和复合物花环(Immu Complex Rosette,ICR)试验证实鸡红细胞表面存在补体C3b受体(C3bR),表明红细胞免疫系统RCIS(Red Cell Immune System)的概念适用于这种动物;并且通过对鸡的白细胞的计数,证明了红细胞免疫吸附性能与白细胞数量之间存在一定的正相关性。     

缺氧条件下FMMU白化豚鼠和杂色豚鼠心肌线粒体形态变化比较研究

目的　比较研究在缺氧条件下FMMU白化豚鼠和杂色豚鼠心肌线粒体的形态变化。方法　将FMMU白化豚鼠和杂色豚鼠置于常压缺氧舱内分别缺氧 7d、14d、2 1d ,复制常压缺氧模型 ,分别取FMMU白化豚鼠和杂色豚鼠心肌 ,电镜下观察心肌线粒体形态的变化。结果　在同一缺氧时间 ,二者的超微结构变化程度不同 ;在不同缺氧时间 ,同种豚鼠的变化程度也不同。结论　在缺氧条件下 ,FMMU白化豚鼠心肌线粒体的变化程度较杂色豚鼠轻 ,提示可能白化豚鼠对缺氧的耐受性优于杂色豚鼠     

花色豚鼠与白化豚鼠静脉血电解质、酸碱平衡及血气的比较学研究

目的比较研究花色豚鼠与白化豚鼠静脉血电解质、酸碱平衡及血气情况。方法分别取健康成年花色豚鼠和白化豚鼠,利用便携式多功能麻醉机经异氟烷吸入麻醉后,腹主静脉取血,经NOVA血气．电解质分析仪全自动分析测定电解质、酸碱平衡及血气等指标。结果花色豚鼠的Cl-、pH、SBC、PO2、SV O2、O2ct均显著低于白化豚鼠（P〈0．05,P〈0．01）,而PCO2显著高于白化豚鼠（P〈O．05）。结论花色豚鼠的携氧能力明显低于白化豚鼠,可以作为血瘀证和亚健康模型研究较好的实验动物。     

Anticomplementary, Anticoagulatory, and Serum-Protein Precipitating Activity of Sodium Polyanetholsulfonate

Sodium polyanetholsulfonate (SPS) at 7.8 mug/ml completely abolished complement-mediated hemolysis of 1:10 diluted fresh guinea pig and human serum; at least twice as much SPS was required to reduce complement activity in 1:2 diluted human serum. The coagulation of 90 and 20% human blood was inhibited by 250 and 125 mug of SPS per ml, respectively. When added to fresh human serum, SPS precipitated beta 1C-globulin (C3), C4, beta lipoproteins, immunoglobulin IgG, IgM, and IgA, though incompletely.     

Analysis of immunoglobulin,complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina)

The northern pig-tailed macaque(NPM,Macaca leonina) has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3-11 year old captive northern pig-tailed macaques using HITACHI 7600-20 automated chemistry analyzer in order to determine the influences of age and gender on these items. The results showed that serum IgA, IgM, C3 and C4 levels were not correlated with age(P0.05), while serum IgG levels increased progressively with age(r=0.202;P=0.045). Serum IgG, IgA, IgM and C3 levels were higher in females than in males(P0.05). Moreover, serum C3 concentration was both positively and strongly correlated with that of C4(r=0.700; P0.0001). This study provides basic serum immunoglobulin and complement data of captive northern pig-tailed macaques, which may prove useful for future breeding efforts and biomedical research.     

Characterization of human T lymphocytes that express the C3b receptor

The presence of the C3b receptor (C3bR) on human peripheral blood T lymphocytes was recognized by the capacity of rabbit F(ab')2 anti-C3bR and tetramethylrhodamine isothiocyanate (TRITC)-conjugated goat F(ab')2 anti-rabbit F(ab')2 to stain 14.5 +/- 3.7% (mean +/- SEM; n = 5) of lymphocytes forming rosettes with sheep erythrocytes (E). The F(ab')2 anti-C3bR also blocked the capacity of peripheral blood lymphocytes stained with OKT11 to form rosettes with bovine E bearing C3b and immunoprecipitated a single membrane protein having a m.w. of approximately 250,000 from detergent lysates of 125I-labeled, purified T cells. Measurement by fluorescent flow cytometry of the quantitative expression of the C3bR indicated that T cells had slightly more antigenic sites/cell than did E and approximately 10-fold fewer sites than were present on B cells. The surface constituents of the peripheral blood T cells expressing the C3bR were assessed in an assay that employed simultaneously three markers: rosette formation with sheep E, TRITC staining with anti-C3bR and fluorescein isothiocyanate (FITC)-staining with a panel of monoclonal antibodies or with aggregated IgG. Among lymphocytes forming rosettes with sheep E and expressing the C3bR, 99.6 +/- 0.4%, 65.0 +/- 5.8%, 17.2 +/- 6.2%, and 15.3 +/- 5.0% of the cells expressed antigens detected by OKT3, OKT4, OKT8, and OKM1 monoclonal antibodies, respectively. Ninety-seven per cent of the C3bR-bearing T cells were also capable of specifically binding aggregated IgG, indicating the presence of Fc receptors for IgG (Fc gamma R) on these cells. The T cells expressing the C3bR had large nuclei, thin rims of basophilic cytoplasm and no azurophilic granules. Thus, the C3bR is present on some T cells, all of which have a typical lymphocyte morphology, the T3 antigen and the Fc gamma R.     

Immunological comparison of basic encephalitogen and histone F2A1.

The extent of immunological cross-reaction between basic encephalitogen and histone F2A1 on both the humoral antibody level and on the cellular level has been established. The extent of humoral cross-reaction was tested by direct complement fixation employing both anti-histone F2A1 and antisera to basic encephalitogen, by inhibition of complement fixation, by radioimmunoassay and by passive cutaneous anaphylaxis. The data obtained failed to reveal immunological cross-reaction at the cellular level was tested by the lymphocyte stimulation technique in rabbits and guinea pigs, by inhibition of lymphocyte stimulation and by delayed hypersensitivity skin reactions. A slight but significant cross-reaction between the two proteins on the cellular level was detected by inhibition of lymphocyte stimulation and by the delayed hypersensitivity test. It is concluded that the immunological studies provide limited evidence that the two proteins share antigenic determinants.     

Humoral components of immunological response in nephrotic syndrome

Serum and urine were collected from 58 patients with nephrotic syndrome. Immunoglobulins (IgA, IgG and IgM), complement (C3) and transferrin levels were measured by single radial immunodiffusion. The extent of glomerular injury was estimated by determining the selectivity of proteinuria. The relationship between the severity of glomerular damage and serum concentrations of immunoglobulins and complement was assessed. Higher IgM and lower IgG serum concentrations were found in nephrotic patients than in normal controls (157 +/- 108 mg+ vs 127 +/- 38 mg% for IgM, 929 +/- 537 mg% for IgG). The difference was statistically significant (p less than 0.05 for IgM, p less than 0.001 for IgG). No correlation was present between the selectivity of proteinuria and serum levels of IgA, IgM, IgG or C3. The results indicate that abnormalities in humoral components of the immune system are present in nephrotic patients and are probably related to a basic immunological defect in the patients rather than to the severity of glomerular damage.     

Participation of complement in nasal polyposis

No changes in immunoglobulin (IgG, IgM and IgA) and complement components C3 and C4 levels could be observed in patients suffering from both recurrent and acute nasal polyposis. However, in both groups of patients a significant decrease of the alternative pathway of complement activation (up to 80%) was found. In addition, patients suffering from the acute disease showed even decreased classical complement pathway (up to 20%). Examination of complement activation pathways was shown to be advantageous for the follow-up of the course of this disease.