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1.
模拟青霉素分批补料发酵过程的细胞自动机模型   总被引:2,自引:0,他引:2  
根据青霉素产生菌的生长机理和青霉素分批补料发酵过程的动力学特性,在Paull等建立的形态学结构动力学模型的基础上,建立了模拟青霉素分批补料发酵过程的细胞自动机模型。模型采用三维细胞自动机作为菌体生长空间,采用Moore型邻域作为细胞邻域,其演化规则根据青霉素分批补料发酵过程中菌体生长机理和简化动力学结构模型设计。模型中的每一个细胞既可代表单个产黄青霉菌体细胞,又可代表特定数量的这种菌体细胞,它具有不同的状态。对模型进行的仿真实验结果表明:模型不但能一致地复现形态学结构动力学模型所描述的青霉素分批补料发酵过程的演化特性,而且较形态学结构动力学模型更加直观地刻画了青霉素分批补料发酵过程的演化行为。最后,对所建模型在实际生产过程中的应用问题进行了分析,指出了需要进一步研究的问题。  相似文献   

2.
构建了重组毕赤酵母产青霉素G酰化酶的分批发酵动力学模型。实验考察了分批发酵过程中甘油消耗、甲醇浓度、菌体浓度、溶氧、补料时间对青霉素G酰化酶活力的影响。应用Matlab软件,对菌体生长、基质消耗和产物生成方程进行最优参数估算和非线性拟合,得到相应的动力学模型。模型的计算值与实验值能较好地拟合,表明所建模型能较好反映重组毕赤酵母产青霉素G酰化酶的分批发酵过程。  相似文献   

3.
基于14L的发酵罐分批发酵实验数据,建立了发酵过程菌体生长、产物生成及基质消耗随时间变化的数学模型。Logistic方程、Luedeking—Piret方程能够很好地分别描述产弹性蛋白酶菌体生长;发酵产酶过程和基质消耗过程。并将3个动力学模型的预测值和实验值进行了比较,所建立的分批发酵动力学模型能较好地反映弹性蛋白酶分批发酵过程。  相似文献   

4.
灵芝胞外多糖分批发酵非结构动力学模型   总被引:8,自引:0,他引:8  
李平作  章克昌 《生物技术》1999,9(3):24-26,34
在2L搅拌发酵罐上提出了描述了灵芝胞外多糖分批发酵过程中菌球生长、底物消耗和胞外多糖形成的非结构动力学模型。首先研究了灵芝分批发酵特性,结果表明该发酵过程属菌体生长和产物形成相偶联型。然后在总结文献的基础上,运用动力学模型,经过非线性回归,得到了模型中的参数值。通过计算机模拟,证明模型预测值与实际实验值具有良好的拟合性。  相似文献   

5.
黑曲霉过氧化氢酶发酵过程的数学模型   总被引:2,自引:0,他引:2  
研究了黑曲霉发酵生产过程氧化氢酶的分批发酵动力学,并建立了发酵过程菌体生长,基质消耗及酶合成的随时间变化的数学模型。Logistic方程,Luedekin-Piret方程及与Luedeking-Piret方程相似的基质消耗方程能够很好地分别描述黑曲霉细胞的生长,发酵产酶过程及葡萄糖的消耗,过氧化氢酶的发酵合成是生长耦联的,研究中还将3个动力学模型的预测值和实验值进行了比较。  相似文献   

6.
Xenorhabdus nematophila发酵动力学研究   总被引:1,自引:0,他引:1  
在分批发酵中,研究了Xenorhabdus nematophila YL001的生长、基质消耗及抗菌物质产生的特性.基于Logistic方程和Luedeking-Piret方程,得到了描述分批发酵过程的动力学模型及模型参数,同时对实验数据与模型进行了验证比较.模型计算值与实验数据拟合良好,模型基本反映了Xenorhabdus nematophila YL001分批发酵过程的动力学特征.分批发酵中细胞生长与产物合成属于偶联型.  相似文献   

7.
目的:研究蓝色犁头霉产壳低聚糖分批发酵动力学的模型.方法:在10L发酵罐中,将蓝色犁头霉分批发酵培养,对蓝色犁头霉菌丝体生长、产物形成和基质消耗的实验数据进行分析,根据Logistic和Luedeking-Piret方程分别建立蓝色犁头霉发酵过程菌体生长、壳低聚糖生成和基质消耗的动力学数学模型,并利用1stOpt软件对模型参数进行非线性曲线拟合.结果:研究得到了菌体生长、产物合成及基质消耗的动力学模型及参数,模型的拟合度分别为0.994、0.986、0.992.结论:研究表明蓝色犁头霉多糖合成和菌体生长呈生长偶联型,模型计算值与实验值有良好的拟合性,模型准确度较高.  相似文献   

8.
通过三联30L全自动发酵罐对虾青素产生菌法夫酵母的分批发酵动力学进行了研究,结果表明,法夫酵母的生长与限制性基质葡萄糖浓度之间符合Logistic方程,建立了细胞生长、产物合成和基质消耗随时间变化的数学模型。应用MATLAB软件对发酵动力学模型进行最优参数估计和非线性拟和,获得最大比生长速率(umax)和产物得率(Yp/x)分别为0.1829/h、0.1524g/g,虾青素分批发酵中细胞生长与产物合成属于偶联型,模型模拟计算结果和实验值能较好地吻合,动力学研究结果表明该模型能较好地反映细胞的生长、底物消耗和产物合成过程机制。  相似文献   

9.
研究了Rhodobacter sphaeroidesEIM-8发酵生产CoQ_(10)的代谢特性.根据Monod、Logistic和Luedeking-Piret方程建立菌体细胞生长、CoQ_(10)积累和葡萄糖消耗的动力学模型,并采用进化规划求得模型参数.模型模拟计算结果与试验值拟合良好,平均相对误差均在7%以内,较好地反映了类球红细菌分批发酵过程的动力学特征.  相似文献   

10.
灵芝胞外多糖分批发酵动力学模型   总被引:7,自引:0,他引:7  
利用分批发酵研究了灵芝(Ganoderma lucidum)胞外多糖的合成特性,结果表明Ganodermalucidum多糖合成和菌体生长呈部分生长关联型。菌体干重、胞外多糖分别达到15.56g·L-1、3.02g·L-1,胞外多糖对细胞干重得率系数(Yp/x)为0.19。根据分批发酵试验结果采用Logistic方程、Luedeking-Piret方程和类似Luedeking-Piret方程,得到了描述灵芝生长、胞外多糖以及葡萄糖底物消耗分批发酵动力学模型。同时在初始葡萄糖变化较大范围内,试验数据与模型预测值进行了比较拟合,平均相对误差小于5%,表现出很好的适用性。表明该动力学模型对指导灵芝胞外多糖的发酵生产具有实际意义。  相似文献   

11.
A cellular automata model to simulate penicillin fed-batch fermentation process(CAPFM)was established in this study,based on a morphologically structured dynamic penicillin production model,that is in turn based on the growth mechanism of penicillin producing microorganisms and the characteristics of penicillin fed-batch fermentation.CAPFM uses the three-dimensional cellular automata as a growth space,and a Moore-type neighborhood as the cellular neighborhood.The transition roles of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes.Every cell of CAPFM represents a single or specific number of penicillin producing microorganisms,and has various state.The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly.  相似文献   

12.
A cellular automata model to simulate penicillin fed-batch fermentation process (CAPFM) was established in this study, based on a morphologically structured dynamic penicillin production model, that is in turn based on the growth mechanism of penicillin producing microorganisms and the characteristics of penicillin fed-batch fermentation. CAPFM uses the three-dimensional cellular automata as a growth space, and a Moore-type neighborhood as the cellular neighborhood. The transition rules of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes. Every cell of CAPFM represents a single or specific number of penicillin producing microorganisms, and has various state. The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly. __________ Translated from ACTA BIOPHYSICA, 2005, 21(2) [译自: 生物物理学报, 2005,21(2)]  相似文献   

13.
温度对谷胱甘肽分批发酵的影响及动力学模型   总被引:18,自引:2,他引:16  
研究了24~32℃范围内产朊假丝酵母生产谷胱甘肽的分批发酵过程,发现较高温度对细胞生长有促进作用,而较低温度则更有利于谷胱甘肽产量的提高。应用改进的Logistic和LuedekingPiret方程分别对细胞生长动力学和谷胱甘肽合成动力学进行了模拟,得到不同温度下各种动力学参数。在此基础上,进一步研究了温度同细胞生长动力学参数之间的内在联系,得到谷胱甘肽分批发酵过程中细胞浓度的变化同温度以及底物浓度之间的一般关系式:dX-dt=[0.0224(T+1.7)]2X(1-X/Xmax)1+S{8.26×10.6×exp[-31477/R/(T+273)]}。验证实验结果表明,该模型具有很好的适用性。  相似文献   

14.
For the purpose of obtaining L-asparaginase in quantities from Erwinia aroideae, cell growth and enzyme formation were investigated in both batch and continuous fermentation. Using yeast extract as a growth-limiting substrate, the relationship between specific growth rate and substrate concentration was found to fit the Monod equation. The optimum temperature for enzyme production was 24 C, although cell growth was higher at 28 C. The enzyme yield reached its maximum of 4 IU/ml during the negative acceleration growth phase which occurs just prior to stationary growth. Compared to batch fermentations, the continuous fermentation process gave a lower enzyme yield except when the fermentation was conducted at a dilution rate of 0.1 hr(-1). The graphical method frequently used for prediction of continuous fermentation does not apply to L-asparaginase production by E. aroideae. The optimum temperature for enzyme production in continuous process was 24 C, which was the same as in batch process. Increasing the temperature from 24 to 28 C resulted in a 20% loss of enzyme yield.  相似文献   

15.
Modeling of yeast metabolism and process dynamics in batch fermentation   总被引:4,自引:0,他引:4  
Much is known about yeast metabolism and the kinetics of industrial batch fermentation processes. In this study, however, we provide the first tool to evaluate the dynamic interaction that exists between them. A stoichiometric model, using wine fermentation as a case study, was constructed to simulate batch cultures of Saccharomyces cerevisiae. Five differential equations describe the evolution of the main metabolites and biomass in the fermentation tank, while a set of underdetermined linear algebraic equations models the pseudo-steady-state microbial metabolism. Specific links between process variables and the reaction rates of metabolic pathways represent microorganism adaptation to environmental changes in the culture. Adaptation requirements to changes in the environment, optimal growth, and homeostasis were set as the physiological objectives. A linear programming routine was used to define optimal metabolic mass flux distribution at each instant throughout the process. The kinetics of the process arise from the dynamic interaction between the environment and metabolic flux distribution. The model assessed the effect of nitrogen starvation and ethanol toxicity in wine fermentation and it was able to simulate fermentation profiles qualitatively, while experimental fermentation yields were reproduced successfully as well.  相似文献   

16.
The full implications of a statistical model for growth of a microbial cell population using cell mass as the index of physiological state have been examined by solving the partial differential integral equations resulting from the model. Calculations reveal that a lag phase is predicted during the initial stages of batch growth although no specific cellular mechanism for the phenomenon of lag had been incorporated into the model. The model predicts several situations of batch and continuous growth in which the population density and biomass concentration show opposing trends due to significant variation in the cell mass distribution with time.  相似文献   

17.
对产酸丙酸杆菌(Propionibacterium acidogenes)FS1171产丙酸的分批发酵动力学进行研究,基于经典发酵动力学模型(Logistic、Luedeking-Piret、Dose-Resp方程)及Origin软件优选模型(Boltzmann方程)两种方法分别构建丙酸发酵过程中菌体生长、丙酸合成及底物甘油消耗随时间变化的动力学模型,软件分析表明,经典发酵动力学构建的模型拟合度整体不如Boltzmann方程构建的动力学模型,但前者所构建的3个动力学模型之间有较好的关联性,两种方法构建的模型拟合值和实验值能较好的吻合,说明所构建的产酸丙酸杆菌的发酵动力学能较好地反应丙酸杆菌的发酵过程,为优化发酵过程和工业上放大生产丙酸提供参考。  相似文献   

18.
The effects of feeding the 'toxic' penicillin precursor, phenylacetic acid (PAA) at varying rates, upon the process of cellular autolysis, was assessed in batch bioreactor cultures of an industrial strain of Penicillium chrysogenum. Five processes were fed at rates which resulted in extracellular concentrations of PAA ranging from zero (the control) to approximately ten times levels said to be optimal for penicillin biosynthesis. The culture response was assessed chemically and morphologically, using computerised image analysis. High concentrations of PAA reduced biomass and penicillin production, and were associated with increased cellular autolysis. However, the values of classical morphological indices (branch length, main hyphal length and hyphal growth unit) varied little in cultures which showed extensive autolysis and biomass loss. Lower precursor concentrations (0.01 to 1.0 g l-1) had little effect on biomass, penicillin, or upon the levels of autolysis compared with the control process. Therefore, precursor concentration controlled within the optimal range for penicillin production, has little impact upon differentiation or degradation within an industrial culture of P. chrysogenum. By contrast, exploitation of the toxicity of PAA is proposed as a means to bring forward or enhance autolysis, providing a reliable method of 'induction' with which to study the phenomenon in P. chrysogenum.  相似文献   

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