柏毒蛾核型多角体病毒的分离鉴定

柏毒蛾核型多角体病毒的分离鉴定李崇荣,彭辉银,周显明,陈新文,谢天恩（贵州省铜仁地区林科所,铜仁５５４３００）（中国科学院武汉病毒研究所,武汉４３００７１）（贵州省林业科学研究院,贵阳５５００１１）关键词柏毒蛾,核型多角体病毒柏毒蛾（Ｐａｒｏｃｅｎｅ...     

复配型苜宿银纹夜蛾核型多角体病毒制剂双两种夜蛾的毒力测定

对防治蔬菜菜夜蛾（Ｓ）和斜纹夜蛾（ＰｒｏｄｅｎｉａｌｉｔｕｒａＦａｂｒｉｃｉｕｓ）为主并兼治菜螟、菜青虫、小菜蛾等鳞翅目害虫的单五型和复配型苜蓿银纹放蛾核型多角体病毒（ＡｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａｎｕｃｈｌｅａｒｐｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ,ＡｃＭＮＰＶ）生物杀虫剂进行筛选。     

三种昆虫核型多角体病毒DNA的限制性内切酶酶解分析

在茶尺蠖、茶毛虫、斜纹夜蛾三种昆虫的四株核型多角体病毒中提取DNA,应用限制性内切酶图谱分析法研究,结果表明三种昆虫核型多角体病毒的内切酶图谱各不相同。而二株茶尺蠖核型多角体病霉的内切酶图谱是一致的。     

棉铃虫核型多角体病毒mRNA的快速制备与体外翻译

棉铃虫核型多角体病毒ｍＲＮＡ的快速制备与体外翻译吴金炉,刘润忠,张友清（中国科学院武汉病毒研究所,武汉４３００７１）关键词棉铃虫核型多角体病毒,ｍＲＮＡ,快速制备,体外翻译,ＥＬＩＳＡ在昆虫杆状病毒ｍＲＮＡ的结构与功能研究方面,主要是以ＡｃＮＰＶ为研...     

粘虫核多角体病毒0.6kb SalI／HindⅢ片段序列的测定及分析

粘虫核多角体病毒０．６ｋｂＳａｌＩ／ＨｉｎｄⅢ片段序列的测定及分析李小锋,王家旺,金海陵,黄永秀,齐义鹏（武汉大学病毒研究所,武汉４３００７２）关键词粘虫,核多角体病毒,序列测定,早期基因核多角体病毒（ＮｕｃｌｅａｒＰｌｏｙｈｅｄｒｏｓｉｓＶｉｒｕｓ...     

灰茶尺蛾核型多角体病毒的精细结构及生物学特性研究

在湖北省茶园害虫灰茶尺蛾(Ectropis grisescens W.)幼虫体内分离到一株核型多角体病毒,属杆状病毒科单粒包埋型。多角体呈不规则的多面体,平均大小为1.76μm。病毒粒子平均大小为287×65nm,粒子两端有环状的帽状结构。测量EgNPV-DNA的分子量约为34×10⁶道尔顿。生物毒力测定其LD₅₆为8.6×10⁵PIB/ml。     

茶毛虫核型多角体病毒（EpNPV）多角体蛋白基因的定位及克隆

茶毛虫核型多角体病毒 (Ｅｕｐｒｏｃｔｉｓｐｓｅｕｄｏｃｏｎｓｐｅｒ ｓａＮｕｃｌｅａｒＰｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ简称ＥｐＭＰＶ) ,属于杆状病毒科核型多角体病毒属 ,能使茶毛虫染病死亡。ＥｐＮＰＶ据报道最初由日本学者于 195 7年发现[1] ,随后在其它国家和地区也有相关报道[2 ] ;我国在贵州、湖北、广西和云南等地也有发现 ,并在ＥｐＮＰＶ病毒杀虫剂的大田应用方面做了大量的工作 ,取得了一定的社会、经济和生态效益[3] 。本文以苜蓿丫纹夜蛾多粒包埋核型多角体病毒(ＡｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａＭＮＰＶ…     

苜蓿尺蠖核型多角体病毒囊膜蛋白

苜蓿尺蠖核型多角体病毒（ Ａｕｔｏｇｒａｐｈａ ｃａｌｉｆｏｒｎｉｃａ ｎｕｃｌｅａｒ ｐｏｌｙｈｅｄｒｏｓｉｓ ｖｉｒｕｓ,ＡｃＭＮＰＶ）在细胞质中合成其囊膜蛋白,但在细胞核内组装并包埋病毒粒子,这些蛋白的核定向转运机制是人们甚感兴趣的课题。以ＡｃＭＮＰＶ多角体衍生型病毒ＯＤＶ（ｏｃｃｌｕｓｉｏｎ－ｄｅｒｉｖｅｄ ｖｉｒｕｓ,ＯＤＶ）的一种囊膜蛋白ＯＤＶ－Ｅ１８为对象,通过Ｅ１８与一个标记短肽Ｆ     

扁刺蛾核型多角体病毒的研究

扁刺蛾(Thosea sinensis Walker)属鳞翅目刺蛾科,危害茶、果、桑等多种经济作物。茶园中扁刺蛾虫害严重时,茶树叶片被吃光,造成茶树枯死。虫体刺毛触及人的皮肤会引起痒痛红肿。1975年9月,我们从敬亭山茶场和舒茶公社茶园自然死亡的扁刺蛾幼虫中,分离到一种核型多角体病毒和一种质型多角体病毒。通过室内感染和田间防治试验表明,扁刺蛾核型多角体病毒的毒力及专一性较强,杀虫效果显著,对家蚕(bombyx mori)和小白鼠无毒。可感染褐刺蛾(Setora postornata Hampson)、绿刺蛾(Porasa lepida Crame)。     

两株甜菜夜蛾核型多角体病毒的研究

本文报道了甜菜夜蛾核型多角体病毒 (Ｓｐｏｄｏｐｔｅｒａｅｘｉｇｕａｎｕｃｌｅａｒｐｏｌｙｈｅｄｒｅｓｉｓｖｉｒｕｓ,ＳｅＮ ＰＶ)美国分离株的形态学观察、宿主范围的测定、活体生物测定及其与甜菜夜蛾核型多角体病毒中山大学分离株的毒力、限制性内切酶图谱比较等研究结果。以喂饲法用ＳｅＮＰＶ美国分离株感染 4龄初甜菜夜蛾幼虫 ,收集具典型病症的虫尸并提纯多角体。取纯多角体进行包埋、切片。电镜观察表明 ,此甜菜夜蛾ＮＰＶ为多粒包埋型核型多角体病毒。用ＳｅＮＰＶ美国分离株以 1 .3× 1 0 7ＰＩＢｓ/ｍＬ分别感染甜菜夜蛾…     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

真菌类遗传学分析的知识结构教学

本文以认知结构理论为指导,讨论了真菌类遗传分析与高等动植物遗传分析的内在联系,认为利用这种内在联系进行教学可收到好的效果并说明了作者的具体教学过程。 Abstract:In the paper, the relationship between genetic analysis of Fungi and genetic analysis of high animal and plant was discussed.A good results were obtained when we adopted this method in the teaching.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

Interconnection of parameters of regulation system of lipid peroxidation and of morphophysiological parameters of mouse liver

A complex analysis of seasonal fluctuations of the mean group parameters of the system of regulation of lipid peroxidation has been performed in liver of Balb/c mice. Association of lipid characteristics and morphophysiological parameters is studied in the Balb/c mouse liver. An inter-connection is revealed between the liver index and the amount of lysoforms of phospholipids, the scale and character of the interconnection differing essentially depending on proportion of phos-phatidylcholine in mouse liver phospholipids.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

Physiological characteristics of various species of strains of carboxydobacteria

Seven strains of aerobic carbon monoxide-oxidizing bacteria (carboxydebacteria) when growing on CO as sole source of carbon and energy had doubling times which ranged from 12–42 h. The activity profiles obtained after discontinuous sucrose density gradient centrifugation indicated that the CO-oxidizing enzymes are soluble and the hydrogenases are membrane-bound in all strains examined. The CO-oxidizing enzymes of Pseudomonas carboxydohydrogena, Pseudomonas carboxydoflava, Comamonas compransoris, and the so far unidentified strains OM2, OM3, and OM4 had a molecular weight of 230,000; that of Achromobacter carboxydus amounted to 170,000. The molecular weights of the CO-oxidizing and H₂-oxidizing enzymes turned out to be identical. The cell sonicates were shown to catalyze the oxidation of both CO and H₂ with methylene blue, thionine, phenazine methosulfate, toluylene blue, dichlorophenolindophenol, cytochrome c or ferricyanide as electron acceptors. Methyl viologen, benzyl viologen, FAD⁺, FMN⁺, and NAD(P)⁺ were not reduced. The spectrum of electron acceptors was identical for all strains tested. Neither free formate, hydrogen nor oxygen gas were involved in the CO-oxidation reaction. Methylene blue was reduced by CO at a 1:1 molar ratio. The results indicate that CO-oxidation by carboxydobacteria is catalyzed by identical or similar enzymes and that the reaction obeys the equation CO+H₂OCO₂+2H⁺+2e^- as previously shown for Pseudomonas carboxydovorans.Dedicated to Otto Kandler remembering almost three decades of enjoyable cooperation     

Modulation of allostery of pyruvate kinase by shifting of an ensemble of microstates

Since the introduction of the concepts of allostery about four decades ago, much advancement has been made in elucidating the structure-function correlation in allostery. However, there are still a number of issues that remain unresolved. In this review we used mammalian pyruvate kinase (PK) as a model system to understand the role of protein dynamics in modulating cooperativity. PK has a triosephosphate isomerase (TIM)(α/β)₈ barrel structural motif. PK is an ideal system to address basic questions regarding regulatory mechanisms about this common (α/β)₈ structural motif. The simplest model accounting for all of the solution thermodynamic and kinetic data on ligand-enzyme interactions involves two conformational states, inactive E^T and active E^R. These conformational states are represented by domain movements. Further studies provide the first evidence for a differential effect of ligand binding on the dynamics of the structural elements, not major secondary structural changes. These data are consistent with our model that allosteric regulation of PK is the consequence of perturbation of the distribution of an ensemble of states in which the inactive E^T and active E^R represent the two extreme end states. Sequence differences and ligands can modulate the distribution of states leading to alterations of functions. The future work includes: defining the network of functionally connected residues; elucidating the chemical principles governing the sequence differences which affect functions; and probing the nature of mutations on the stability of the secondary structural elements, which in turn modulate allostery.