首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 156 毫秒
1.
贵州从江侗族Y-DNA及线粒体DNA 序列多态性分析   总被引:6,自引:3,他引:3  
为分析贵州从江侗族父系及母系遗传结构,探讨其起源及迁徒, 通过聚合酶链式反应-限制性片段长度多态性(PCR-RFLP), 研究贵州从江侗族无亲缘关系个体由10个单核苷酸位点(SNPs)组成的Y染色体单倍型及11个单核苷酸位点组成的线粒体DNA单倍群频率。结果显示, 从40份男性样本的Y-SNP基因分型中,得到H6 、H11、H14 共3种单倍型;H11的频率为92.5%;通过对线粒体DNA基因分型,得到6种单倍群,有75%的个体能明确分类其所携带的单倍群特征,说明贵州从江侗族父系遗传构成相对简单。通过主成分分析,证明贵州从江侗族与其他的壮侗语族人群相聚,母系遗传结构复杂,无C单倍群分布可能为该民族特征之一。Abstract: To study the patrilineal and matrilineal genetic structure and the origin of Dong Ethnic of Congjiang Guizhou. Study the distribution of Y-chromosome haplotypes which consisted of 10 SNPs of Y-DNA and mtDNA haplogroups consisted of 11 SNPs by using PCR-RFLP method. The result is three haplotypes H6,H11,H14 were detected, the frequency of H11 is 92.5%. Six haplogroups were identified by mtDNA analysis, 75% of the people can be identified. The patrilineal genetic structure of Dong of Guizhou is simple, Principle component indicated that the structure is closer to Zhuang-Dong branch of Sino-Tibetan language family. The matrilineal genetic structure of Dong of Guizhou is complicated.  相似文献   

2.
Wang  Shi  Jiao  Ni  Zhao  Lu  Zhang  Meiwen  Zhou  Pei  Huang  Xuexue  Hu  Fangzhou  Yang  Conghui  Shu  Yuqin  Li  Wuhui  Zhang  Chun  Tao  Min  Chen  Bo  Ma  Ming  Liu  Shaojun 《中国科学:生命科学英文版》2020,63(1):102-115
In terms of taxonomic status, common carp(Cyprinus carpio, Cyprininae) and crucian carp(Carassius auratus, Cyprininae) are different species; however, in this study, a newborn homodiploid crucian carp-like fish(2n=100)(2nNCRC) lineage(F1–F3) was established from the interspecific hybridization of female common carp(2n=100)×male blunt snout bream(Megalobrama amblycephala, Cultrinae, 2n=48). The phenotypes and genotypes of 2 n NCRC differed from those of its parents but were closely related to those of the existing diploid crucian carp. We further sequenced the whole mitochondrial(mt) genomes of the 2n NCRC lineage from F1 to F3. The paternal mt DNA fragments were stably embedded in the mt-genomes of F_1–F_3 generations of 2n NCRC to form chimeric DNA fragments. Along with this chimeric process, numerous base sites of F1–F3 generations of 2 n NCRC underwent mutations. Most of these mutation sites were consistent with the existing diploid crucian carp. Moreover, the mt DNA organization and nucleotide composition of 2n NCRC were more similar to those of the existing diploid crucian carp than those of the parents. The inheritable chimeric DNA fragments and mutant loci in the mt-genomes of different generations of 2nNCRC provided important evidence of the mt DNA change process in the newborn lineage derived from hybridization of different species. Our findings demonstrated for the first time that the paternal mt DNA were transmitted into the mt-genomes of homodiploid lineage, which provided new insights into the existence of paternal mt DNA in the mt DNA inheritance.  相似文献   

3.
This paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B (apoB) gene (SP-I/D, XbaI-RFLP, VNTR) and natural longevity persons among the Uygur people in Xin-jiang. For this purpose, 191 healthy Uygur individuals aged above 90 from Hetian area of Xinjiang were recruited, and another 53 persons aged 65—70 from the same nationality, the same region and with the same gender ratio, served as the control group. Genotyping was performed by PCR-SSP, PCR-RFLP and PCR-sequencing methods. Logistic regression analyses revealed that the frequencies of X X genotype, M and L alleles and the genetypes composed of M and L were significantly higher in the longevity group than in the control group. In haplotype analyses, we found that, in the long-lived people, the frequency of haplotypes composed of the X and M alleles was significantly higher whereas the frequency of haplotypes composed of the X- and S alleles was significantly lower (both P<0.05) I than those of their controls. These results indicated that the S allele, SS genotype and X -S, D-S, D-X -S haplotypes were the possible adverse factors, whereas the M, L alleles, X X , MM, ML, LL genotypes and I-X -M, X -M haplotypes were the possibe protective factors for the naturally long-lived Uygur people in China.  相似文献   

4.
We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.  相似文献   

5.
We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903:±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.  相似文献   

6.
The Han Chinese people are the main ethnic group in China and the largest ethnic group in the world.The dimensions of the hands and feet have been successfully used for the estimation of stature.A total of 26,927 healthy adult subjects,comprising 13,221 men and 13,706 women,were recruited.The survey samples were chosen through random cluster sampling.The mean values were significantly higher in men than those in women for all measurements(P0.001).All the measurements showed a statistically significant correlation with stature(P0.01).The combination of the right hand length and the right foot length was the best predictor of stature because it had the lowest standard error of estimate.The use of multiple regression equations yielded better results than did the use of linear regression equations.The accuracy of stature prediction ranged from ±4.81 to ±6.39 cm.The present study was of great importance with regards to improving the physical anthropology database of ethnic groups in China.  相似文献   

7.
This paper reports the confirmed diagnosis by nested RT-PCR of PPR cases in Tibet, China in 2007, and results of phylogenetic analysis. Results showed that the 11 tested samples were PPRV positive by nested RT-PCR, of which 2 samples were genetically close to the X7443 strain (Nigeria 75/1) of lineage I, and 3 samples close to the strain AY560591 (Sungri96) of linage IV with 96.6%, 97.3%, 97.6% and 98% nucleotide sequence homogeneity respectively, based on partial sequencing of the F gene from 5 samples and complete sequencing of the N/M/F/H genes from one sample. This study suggested that there are at least 2 origins of PPRV in China.  相似文献   

8.
We investigated the distribution of Y-chromosome haplotype using 19 Y-SNPs in Han Chinese populations from 22 provinces of China. Our data indicate distinctive patterns of Y chromosome between southern and northern Han Chinese populations. The southern populations are much more polymorphic than northern populations. The latter has only a subset of the southern haplotypes. This result confirms the genetic difference observed between southern and northern ethnic populations in East Asia. It supports the hypothesis that the first settlement of modern humans of African origin occurred in the southern part of East Asia during the last Ice Age, and a northward migration led to the peopling of northern China.  相似文献   

9.
To evaluate the gene admixture on the current genetic landscape in Gansu Corridor (GC) in China, the upper part of the ancient Silk Road which connects the Eastern and Central Asia, we examined mitochondrial DNA (mtDNA) polymorphisms of five ethnic populations in this study. Using PCR-RFLP and sequencing, we analyzed mtDNA haplotypes in 242 unrelated samples in three ethnic populations from the GC region and two ethnic populations from the adjacent Xinjiang Uygur Autonomous Region of China. We analyzed the data in comparison with the previously reported data from Eastern, Central and Western Asia and Europe. We found that both European-specific haplogroups and Eastern Asian-specific haplogroups exist in the Gansu Corridor populations, while a modest matrilineal gene flow from Europeans to this region was revealed. The Gansu Corridor populations are genetically located between Eastern Asians and Central Asians, both of who contributed significantly to the maternal lineages of the GC populations. This study made the landscape of the gene flow and admixture along the Silk Road from Europe, through Central Asia, to the upper part of the Silk Road more complete.  相似文献   

10.
Calotes versicolor Daudin,1802,is geographically widespread along the Mekong River basin.The Mekong River is play important role as a significant natural barrier to several terrestrial animals living on different sides.This study aims to analyze the genetic diversity and population structure of C.versicolor populations collected from different sides of Mekong River using mitochondrial cytochrome c oxidase subunit 1(CO1)sequences.We obtained sequences of 200 individuals from 18 sampling localities from left and right sides of the Mekong River in Lao PDR and Thailand respectively.Overall,91 haplotypes were detected,which reflect high levels of genetic diversity in this species at the study areas.Haplotype network and phylogenetic analyses revealed that there were six major lineages(lineage C–lineage H)of C.versicolor populations within the Mekong River,whereas lineages A and B have previously been found from China and Vietnam.The genetic distance among C.versicolor was significantly related to spatial distance,however,the Mekong River had no significant effect on genetic distance.Our findings,together with previous studies,suggests that C.versicolor in Asia is a species complex with other cryptic lineages being likely but there is a need for further exploration.Thus,comprehensive genetic,biological and ecological studies of C.versicolor should be conducted throughout its entire distribution range.  相似文献   

11.
从父系和母系基因库水平上,研究不同分布地区白族群体之间的遗传结构的异同,并对其族源以及本民族群体之间的微进化关系进行初步的探讨。利用PCR-RFLP方法对云南白族和湖南白族及云南的傣族、布依族、独龙族、怒族、阿昌族和湖南土家族共8个群体进行14个线粒体多态位点和Y染色体上的13个双等位基因位点进行基因分型。统计单倍型,在SPSS软件上进行主成分分析。结果显示,两个白族群体在Y染色体双等位基因单倍型分布上差异不大,以H6、H8为主要单倍型分布;在线粒体单倍群分布上,两个白族群体则差异显著,单倍群D、B、M8在湖南白族中的分布频率比云南白族高的多,而在云南白族中M^*、G、F的频率则比湖南白族高。对Y染色体单倍型分布频率进行主成分分析表明两个白族群体聚在一起,整体上和其他北方起源的群体聚成一组;而对线粒体的单倍群分布频率分析显示湖南白族接近湖南汉族和土家族,而云南白族则接近云南怒族和阿昌族。两个白族群体在父系遗传结构上相近,表明他们具有共同的父系族源;而母系遗传结构上的差异,可能与历史上迁到湖南的白族先民主要为男性军士,流寓到当地后与汉、土家等民族女子通婚所致。  相似文献   

12.
云南18个民族Y染色体双等位基因单倍型频率的主成分分析   总被引:10,自引:2,他引:8  
世居云南的少数民族中。壮、傣、水、布依、布朗、德昂、佤、彝、白、怒、哈尼、傈僳、拉祜、纳西、景颇、阿昌、基诺和独龙18个民族是由“羌”、“濮”、“越”3大部落群体演化而来,是云南的土著居民。利用PCR-RFLP方法对这18个土著民族进行Y染色体上13个双等位基因位点进行基因分型。结果显示,不同历史族源的民族群体在Y染色体双等位基因单倍型分布上具有一定的差异:在百越后裔民族群体中以单倍型H11、H12为主要分布;在氐羌后裔民族中以单倍型H5、H6和H8为主要分布;在百濮后裔民族群体中主要单倍型分布为H6、H8和H11。进一步主成分分析表明,百越后裔民族群体和氐羌后裔民族在主成分图上聚为两组,提示父系基因库有不同的来源,与历史记载相印证。  相似文献   

13.
刘烜  单可人  齐晓岚  何燕  赵艳  吴昌学  李毅  褚迅  任锡麟 《遗传》2006,28(11):1350-1354
为分析贵州布依族、仡佬族、仫佬族、毛南族、壮族父系遗传结构, 探讨其起源及迁徒。通过聚合酶链式反应-限制性内切酶长度多态性(PCR-RFLP)方法检测贵州境内5个民族10个SNP位点构成的Y染色体单倍型, 并以省内苗族为对照分析其父系遗传结构。结果显示5个民族集中于Y-SNP中H8单倍型, 苗族样本集中于Y-SNP中的H8、H11与H12单倍型。说明贵州省布依族、仡佬族、仫佬族、毛南族、壮族5个民族之间有密切联系, 且与国内其他地域有较大的遗传差异, 是一个相对独立的群体。  相似文献   

14.
贵州瑶族3支系Y-DNA及线粒体DNA序列多态性分析   总被引:4,自引:0,他引:4  
褚迅  单可人  文波  齐晓岚  李毅  吴昌学  刘烜  赵艳  任锡麟  金力 《遗传》2006,28(2):153-158
采用PCR-RFLP技术,通过观察由12个单核苷酸多态位点(SNPs)组成的Y染色体单倍型及由9个多态位点组成的线粒体DNA单倍型在贵州瑶族中的分布,分析贵州瑶族父系及母系遗传结构,探讨其起源及迁徙。结果显示,97份男性样本分别属于H7、H8、H9、H11 4种Y-DNA单倍型,苗瑶语系特异Y-DNA单倍型H7的平均频率为92.4%;通过对线粒体DNA基因分型,得到8种单倍型,可归入B4、B5、D4、D5和N*单倍型类群中,CoⅡ/tRNALys区域间的9bp缺失平均频率为58.2%。结果提示贵州瑶族父系遗传结构单一,具有典型的苗瑶族群特征,又存在与其他族群的融合。母系遗传结构相对复杂,9 bp缺失是贵州瑶族的母系遗传结构特征。   相似文献   

15.
世界秋海棠属侧膜组植物新资料   总被引:2,自引:0,他引:2  
记述了秋海棠属侧膜组11新种,即星果草叶秋海棠(BegoniaasteropyrifoliaY.M.ShuietW.H.Chen)、耳托秋海棠(B.auritistipulaY.M.ShuietW.H.Chen)、桂南秋海棠(B.austroguangxiensisY.M.ShuietW.H.Chen)、水晶秋海棠(B.crystallinaY.M.ShuietW.H.Chen)、须苞秋海棠(B.fimbribracteataY.M.ShuietW.H.Chen)、巨叶秋海棠(B.gigaphyllaY.M.ShuietW.H.Chen)、黄氏秋海棠(B.huangiiY.M.ShuietW.H.Chen)、长柱秋海棠(B.longistylaY.M.ShuietW.H.Chen)、扁果秋海棠(B.platycarpaY.M.ShuietW.H.Chen)、喙果秋海棠(B.rhynchocarpaY.M.ShuietW.H.Chen)、多变秋海棠(B.variifoliaY.M.ShuietW.H.Chen);并报道了3新变种及1新名称,即疏毛越南秋海棠(B.boniiGagnep.var.remotisetulosaY.M.ShuietW.H.Chen)、密毛龙州秋海棠(B.morseiIrmsch.var.myriotrichaY.M.ShuietW.H.Chen)、簇毛伞叶秋海棠(B.umbr-aculifoliaY.WanetB.N.Changvar.flocculosaY.M.ShuietW.H.Chen)及彩纹秋海棠(B.variegataY.M.ShuietW.H.Chen)。  相似文献   

16.
李永念  左丽  文波  柯越海  黄薇  金力 《遗传学报》2002,29(3):196-200
为探讨中国布依族人的起源及迁移,采用PCR-RFLP法观察了由13个单核苷酸多态位点(SNPs)组成的Y染色体单倍型在中国布依族人群中的分布,同时用PCR直接测序法对其线粒体DNARegionV区多态进行检测,将结果与我国其他民族及世界各大洲人群进行比较,结果表明中国布依族人的单倍型分布与我国同属侗傣语系的壮族、侗族,黎族及金秀的瑶族最为接近,提示布依族人与上述人群有一定的亲缘关系,并结合文史资料,对中国布依族人的起源及迁移进行了初步探讨。  相似文献   

17.
DNA variation on the non-recombining portion of the Y chromosome was examined in 610 male samples from 14 global populations in north, east, and southeast Asia, and other regions of the world. Eight haplotypes were observed by analyses of seven biallelic polymorphic markers ( DYS257(108), DYS287, SRY(4064), SRY(10831), RPS4Y(711), M9, and M15) and were unevenly distributed among the populations. Maximum parsimony tree for the eight haplotypes showed that these haplotypes could be classified into four distinct lineages characterized by three key mutations: an insertion of the Y Alu polymorphic (YAP) element at DYS287, a C-to-G transversion at M9, and a C-to-T transition at RPS4Y(711). Of the four lineages, three major lineages (defined by the allele of YAP(+), M9-G, and RPS4Y-T, respectively) accounted for 98.6% of the Asian populations studied, indicating that these three paternal lineages have contributed to the formation of modern Asian populations. Moreover, phylogenetic analysis revealed three monophyletic Asian clusters, which consisted of north Asian, Japanese, and Han Chinese/southeast Asian populations, respectively. Coalescence analysis in the haplotype tree showed that the estimated ages for three key mutations ranged from 53,000 to 95,000 years, suggesting that the three lineages were separated from one another during early stages of human evolutionary history. The distribution patterns of the Y-haplotypes and mutational ages for the key markers suggest that three major groups with different paternal ancestries separately migrated to prehistoric east and southeast Asia.  相似文献   

18.
The Y chromosomes of 549 individuals from Siberia and the Americas were analyzed for 12 biallelic markers, which defined 15 haplogroups. The addition of four microsatellite markers increased the number of haplotypes to 111. The major Native American founding lineage, haplogroup M3, accounted for 66% of male Y chromosomes and was defined by the biallelic markers M89, M9, M45, and M3. The founder haplotype also harbored the microsatellite alleles DYS19 (10 repeats), DYS388 (11 repeats), DYS390 (11 repeats), and DYS391 (10 repeats). In Siberia, the M3 haplogroup was confined to the Chukotka peninsula, adjacent to Alaska. The second major group of Native American Y chromosomes, haplogroup M45, accounted for about one-quarter of male lineages. M45 was subdivided by the biallelic marker M173 and by the four microsatellite loci alleles into two major subdivisions: M45a, which is found throughout the Americas, and M45b, which incorporates the M173 variant and is concentrated in North and Central America. In Siberia, M45a haplotypes, including the direct ancestor of haplogroup M3, are concentrated in Middle Siberia, whereas M45b haplotypes are found in the Lower Amur River and Sea of Okhotsk regions of eastern Siberia. Among the remaining 5% of Native American Y chromosomes is haplogroup RPS4Y-T, found in North America. In Siberia, this haplogroup, along with haplogroup M45b, is concentrated in the Lower Amur River/Sea of Okhotsk region. These data suggest that Native American male lineages were derived from two major Siberian migrations. The first migration originated in southern Middle Siberia with the founding haplotype M45a (10-11-11-10). In Beringia, this gave rise to the predominant Native American lineage, M3 (10-11-11-10), which crossed into the New World. A later migration came from the Lower Amur/Sea of Okhkotsk region, bringing haplogroup RPS4Y-T and subhaplogroup M45b, with its associated M173 variant. This migration event contributed to the modern genetic pool of the Na-Dene and Amerinds of North and Central America.  相似文献   

19.
The effect of sex-biased dispersal in mammalian ecology and evolution can be elucidated by focusing on maternally or paternally inherited DNA polymorphisms. In sika deer, the genetic structure of the maternal lineage has been clarified by studies based on mitochondrial DNA variation. However, the genetic structure of the paternal lineage has not been well analyzed due to the limited number of point mutations in Y chromosome sequences. In this study, we focused on mutations of highly polymorphic simple sequence repeats (SSRs) in the Y chromosome and developed 16 Y chromosome SSR markers to evaluate male-biased dispersal in sika deer. In total, 55 alleles and 31 multi-locus haplotypes were detected from these 16 loci, revealing clear genetic differentiation among populations (FST = 0.783). In particular, there were unique alleles for the native individuals on Tanegashima and Yakushima Islands and introduced exotic individuals from Taiwan. These markers are highly useful for evaluating not only historical male-mediated dispersal, genetic structure, and demography of the native populations in Japan, but also the impact of artificial introductions on hybridization, especially the introgression of alleles from escaped farmed individuals to native populations.  相似文献   

20.
In a large variety of genetic studies, probabilistic inferences are made based on information available in population databases. The accuracy of the estimates based on population samples are highly dependent on the number of chromosomes being analyzed as well as the correct representation of the reference population. For frequency calculations the size of a database is especially critical for haploid markers, and for countries with complex admixture histories it is important to assess possible substructure effects that can influence the coverage of the database. Aiming to establish a representative Brazilian population database for haplotypes based on 23 Y chromosome STRs, more than 2,500 Y chromosomes belonging to Brazilian, European and African populations were analyzed. No matter the differences in the colonization history of the five geopolitical regions that currently exist in Brazil, for the Y chromosome haplotypes of the 23 studied Y-STRs, a lack of genetic heterogeneity was found, together with a predominance of European male lineages in all regions of the country. Therefore, if we do not consider the diverse Native American or Afro-descendent isolates, which are spread through the country, a single Y chromosome haplotype frequency database will adequately represent the urban populations in Brazil. In comparison to the most commonly studied group of 17 Y-STRs, the 23 markers included in this work allowed a high discrimination capacity between haplotypes from non-related individuals within a population and also increased the capacity to discriminate between paternal relatives. Nevertheless, the expected haplotype mutation rate is still not enough to distinguish the Y chromosome profiles of paternally related individuals. Indeed, even for rapidly mutating Y-STRs, a very large number of markers will be necessary to differentiate male lineages from paternal relatives.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号