多基因联合分析棒束孢属Isaria（Ascomycota,Cordycipitaceae）系统发育关系

对棒束孢属Isaria及近缘属物种开展5基因（nrSSU、nrLSU、tef-1α、rpb1和 rpb2）测序并联合分析,结合GenBank相关类群序列,探讨棒束孢属系统发育关系,最终获得95个菌株、58个明确分类群的2-5基因序列。利用MEGA和MrBayes软件进行多基因聚类分析,结果表明棒束孢属多系起源于虫草菌科中,分3个不同分支。A支主要由Isaria cicadae、I. teniupes、I. coleopterorum、I. fumosorosea和I. cateniannulata等组成;B支包括I. poprawkii、I. locustica、I. javanica、I. amoenerosea和I. cateniobliqua;C支仅有I. farinosa。分支间被Cordyceps militaris、C. ninchukispora、C. pruinosa等隔开。棒束孢在形态上,主要以瓶梗基部膨大、尖端变细及孢子呈链状等特征与其他类群分开,但同时也发现有棒状分生孢子梗和单孢子类型。基于节点的分歧时间预测分析,推测棒束孢属首次分化于70Mya,但棒束孢属主要物种形成却在60-55Mya,且3个分支的棒束孢物种为快速同时形成,而后大多数类群表现遗传稳定。同时发现,与Isaria Clade A较近一支有粉被玛利亚霉Mariannaea pruinosa（C. pruinosa无性型）和蛹草蚧霉Lecanicillium militaris（C. militaris无性型）;与粉棒束孢距离最近一支有Akanthomyces aculeatus（C. tuberculata无性型）和L. attenuatum（C. confragosa无性型）,是两个不同的属征分类群,且相互间遗传距离较近。根据棒束孢属及其近缘种属形态特征的复杂性推测,棒束孢属在快速物种形成中,其近缘类群存在一定程度的丢失和选择性演化。     

浙江凤仙花属的订正——国产凤仙花属植物的研究(二)

作者对浙江的凤仙花属进行了订正, 共记载有11种, 除描述了Impatiens chekiangensis, I.platysepala, I.chlroxantha和I.tienmushanica 4新种外, 还有3个新记录, 对I.cosmia Hook.f.作了归并, 并附有分种检索表, 本文是《国产凤仙花属植物研究》的续篇.     

中国(竹思)簩竹属(Schizostachyum Nees)的研究及其它

对国产(竹思)(竹劳)竹属(Schizostachyum Nees)进行了系统整理。对属的范围进行了修订,认为本属应包括乔草竹属(Dendrochloa Parkinson),薄竹属(Leptocanna Chia et H.L.Fung),长穗竹属(Teinostachyum Munro)和李海竹属(Neohouzeaua A.Camus),并首次建立了本属的属下分类系统。此外,对二种竹子起了新名:甲竹(Bambusa austro-sinensis Xia)和毛环单竹(B.yunnanensis Xia);作了4个新组合,即Schizostachyum coradatum(Wen et Dai)Xia, S.dumetorum(Hance)Munro var.xinwuense(Wen et J. Y Chin)Xia, Bambusa glaucescens glaucescens(Willd)Sieb. ex Munro var.annulata(W.T.Lin et Z.J.Feng)Xia和B.glaucescens (Willd)Sieb.ex Munro var.pubivagina(W.T.Lin et Z.J.Feng)Xia.     

中国马兜铃属和关木通属(马兜铃科)概览

马兜铃属(广义) (Aristolochia sensu lato)具有花单被、花萼管状、合蕊柱、子房下位、中轴胎座、胚珠多数、蒴果等主要特征, 广布于全世界的热带、亚热带和温带地区, 约有600种, 是马兜铃科中种类最多的属。依据Flora of China, 我国有本属植物45种, 其中33种为中国特有。近些年, 国内大量的新类群被相继报道, 特别是云南、广西两地, 而另一些类群则得到了确认、恢复、重新发表或修订。最近, 基于形态和分子证据, 关木通属(Isotrema)因其花萼管急剧弯曲; 合蕊柱3裂; 雄蕊6, 成对与合蕊柱裂片对生; 蒴果由上而下开裂等区别特征而从广义马兜铃属中被分出独立成属。本文基于大量的野外调查、标本鉴定、数码照片考证和相关文献的仔细研究, 重新梳理了中国马兜铃属和关木通属的种类及分布情况, 确认现阶段中国马兜铃属17种和关木通属58种1亚种, 并一一记述同时编制了最新的检索表。其中, 柔叶关木通(I. mollis)、线叶关木通(I. neolongifolia)的种级地位得到重新确认, 并提供了图版。探讨了优贵马兜铃(A. gentilis)、川滇马兜铃(A. chuandianensis)和纤细马兜铃(A. gracillima)的关系, 昆明关木通(I. kunmingense)与波氏关木通(A. bonatii)的关系, 以及卵叶关木通(I. ovatifolium)、葫芦叶关木通(I. cucurbitoides)、西藏关木通(I. griffithii)、过石珠(I. versicolor)、大别山关木通(I. dabieshanensis)等复合群和袋形关木通(I. saccata)等物种存在的问题。     

中国(竹思)簩竹属(Schizostachyum Nees)的研究及其它

对国产(竹思)(竹劳)竹属(Schizostachyum Nees)进行了系统整理。对属的范围进行了修订,认为本属应包括乔草竹属(Dendrochloa Parkinson),薄竹属(Leptocanna Chia et H.L.Fung),长穗竹属(Teinostachyum Munro)和李海竹属(Neohouzeaua A.Camus),并首次建立了本属的属下分类系统。此外,对二种竹子起了新名:甲竹(Bambusa austro-sinensis Xia)和毛环单竹(B.yunnanensis Xia);作了4个新组合,即Schizostachyum coradatum(Wen et Dai)Xia, S.dumetorum(Hance)Munro var.xinwuense(Wen et J. Y Chin)Xia, Bambusa glaucescens glaucescens(Willd)Sieb. ex Munro var.annulata(W.T.Lin et Z.J.Feng)Xia和B.glaucescens (Willd)Sieb.ex Munro var.pubivagina(W.T....     

竹亚科刚竹属植物的修订(I)

在标本 (包括许多模式标本 )查考和居群调研的基础上 ,对刚竹属 PhyllostachysSieb.et Zucc.一些类群的分类问题进行了评注 ,其中将遂昌雷竹 Ph.primotina Wen作为不同于红壳雷竹 Ph.incarnata Wen的 1个独立的竹种看待 ;8个拉丁学名被减为异名 ;Ph.heteroclada Oliv.f.solida( S.L .Chen) C.P.Wang et Z.H.Yu因其基名无拉丁描述 ,故予废弃 ;同时还描述了 1个新变型。     

白囊耙齿菌对茜素红脱色条件优化及其毒性变化检测

白囊耙齿菌Irpex lacteus是分离自倒木上的一株可以分泌漆酶和锰过氧化物酶的白腐真菌。利用I. lacteus对固体条件下的活性黑、活性红、结晶紫、茜素红和孔雀石绿进行脱色能力的检测,通过单因素和正交试验优化I. lacteus对茜素红的脱色条件,并以3种作物发芽率为指标测定茜素红被I. lacteus脱色前后的毒性变化。结果显示,I. lacteus对5种染料均可脱色,其中对茜素红染料的脱色更为彻底;单因素和正交试验优化I. lacteus对茜素红的脱色条件为：pH 7.0、葡萄糖10.0g/L、硫酸铵0.66g/L、接种量2片（Φ=8.0mm）、100.0mL三角瓶装液20.0mL,优化条件下I. lacteus对茜素红脱色10d时的脱色率为88.26%,与未优化前的脱色率相比提高了60.50%;茜素红染料被I. lacteus脱色前后毒性大小排序为：染料原液>染料脱色后>PDB培养基处理,表明茜素红染料存在一定的毒性,I. lacteus脱色茜素红后可以使其毒性减弱。通过本研究,为I. lacteus在茜素红等染料废水脱色以及降低染料废水毒性方面的应用奠定基础。     

广东竹亚科新组合及新异名

报道了广东竹亚科13新组合,1新名称和3新异名,其中筋竹属3新组合1新名称,牡竹属4新组合,青篱竹属5组合1新异名,箬竹属2新异名及方竹属1新组合.     

斑苦竹学名的订正

作者研究了斑苦竹后认为, 当斑苦竹组合到青篱竹属Arundinaria 时, 其合法双名应为Arundinaria oleosa (Wen)C.S.Chao et G.Y.Yang。而Arundinaria chinensis C.S.Chao et G.Y.Yang 和Acidosasa lentiginosa W.T.Lin et Z.J.Feng 为斑苦竹的新异名。     

Chi竹属和牡竹属（竹亚科）叶表皮微形态特征

利用光学显微镜和扫描电镜,对Chi竹属21个种（包括Chi竹亚属5个种、孝顺竹亚属2种、单竹亚属4种绿竹亚属9种和亚属未定1种）以及牡竹属5种叶表皮微形态特征进行了观察和研究。所得结果表明气孔保卫细胞上乳突的数目和分布存在一定的规律,并具有一定的分类价值。绿竹亚属的叶表皮微形态特征界于Chi竹属和牡竹属之间,但更接近于Chi竹属。因此认为在分类处理上,将绿竹亚属置于Chi竹属比单独成立绿竹属更为合理。     

On the identity of the genus Metasasa W. T. Lin

In this paper, Metasasa W. T. Lin was treated as a new synonym of the genus Acidosasa C. D. Chu et C. S. Chao ex Keng f. A new combination, Acidosasa nanunica (McCl.) C. S. Chao et G. Y. Yang, was given. Metasasa carinata W. T. Lin and Metasasa albo-farinosa W.T. Lin were reduced as synonyms of A. nanunica.     

异枝竹属的名实问题

将异枝竹属Metasasa W．T. Lin作为酸竹属Acidosasa C．D．Chu et C．S．Chao ex Keng f．的异名处 理。异枝竹Metasasa carinata W．T. Lin和白环异枝竹 Metasasa albo-farinosa W．T．Lin作为新组合名Aci-dosasa nanunica(McCl．)C．S．Chao et G．Y．Yang,comb．nov．的异名。     

A Study on the Genus Acidosasa of Bambusoideae

The genus Acidosasa was published by the present authors in 1979. It only had one species at that time, Acidosasa chinensis C. D. Chu et C. S. Chao. Since then species number of the genus steadily increases. The authors have rather comprehensively studied this genus and its related genera for F1. Reip. Pop. Sin. The present paper deals mainly with morphological characteristics of the genus Acidosasa and the differences from its related genera i. e. Arundinaria, Sasa and Indosasa. The genus Acidosasa is closely related to the genus Arundinaria in the type and origin of inflorescences and the vegetative appearance. But it differs from Arundinaria in the structure of florets. In Acidosasa, each floret is provided with six stamens, while in Arundinaria each floret is of only three stamens. The genus Acidosasa is similar to the genera Indosasa and Sasa in the numbers of stamens, but it is distinguished from lndosasa by its semelauctant (determinate) inflorescence, not iterautant (indeterminate) one, from Sasa by its taller stature and branch complement with three branches. We have carefully examined all the type specimens of Acidosasa and its related genera. A conclusion reached is that there are six species in the genus Acidosasa, most of which are native to S. China, with only one species in Viet Nam. Five specific binomials are reduced and one species is transferred into this genus. Two keys to species, respectively based on the flowering and vegetative characters, are given as follow: Key to species of the genus A cidosasa (1)(based on the flowering state) 1. Lemmas glabrous. 2. Spikelets stout, 3-6mm broad, pedicels 1.5-4cm long; lemmas large, 1.5-2.2cm long, with 15-19 nerves, subcoriaceous, not glaucous, shiny. 3. Lemmas up to 2.2cm long with conspicuously transverse veinlets, tessellate; palea and rhachilla entirely. glabrous, lodicules elliptic-lanceolate, glabrous ... 1. A. chinensis 3. Lemmas 1.5-1.8cm. long, slightly tessellate; palea puberulous at apex of carina, rhachilla puberuous at apex, lodicules obovate, ciliate at apex ............ 2. A. brilletii 2. Spikelets rather slende, 2-4mm broad, pedicels 0.5-1cm long; lemmas small, about 1.3 cm long, with 7-13 nerves, more or less glaucous .......... 3. A. chienouensis 1. Lemmas pubescent. 4. Glumas and lemmas densely pubescent ........................ 4. A. hirtiflora 4. Glumas subglabrous, lemmas sparsely pubescent. 5. Spikelets large, 3-7 cm long, lemmas 1.6-1.7 cm long, pedicels 2-13 mm long ................................................. 5. A. longiligula 5. Spikelets small, 2-3.7 cm long, lemmas about 1.3 cm long, pedicels 1-3 cm long ................................................... 6. A. venusta Key to species of the genus A cidosasa (2) (based on the vegetative state) 1. Ligules of leaf-sheaths strongly elevated, usually 2-8 mm long. 2. Young culms with bristly sheath scars; culm-sheaths without auricles and oral setae, not spotted, sheath-blades erect ................. 4. A. hirtiflora 2. Young culms with glabrous sheath scars; culm-sheaths with small auricles and oral setae, sparsely spotted, sheath-blades reflexed .......... 5. A. longiligula 1. Ligules of leaf-sheaths inconspicuous, less than 2 mm long. 3. Young culms more or less bristly, or sheath-scars bristly: 4. Culm-sheaths without auricles and oral setae, not farinose, without hairs at base. 5. Young culms densely bristly; culm-sheaths attenuate at apex and as wide as sheath-blades, with conspicuously transverse veinlets; leaf-blades large, usually 2.5-3.5 (-6.5) cm broad, conspicuously tessellate ..................................................... 1. A. chinensis 5. Young culms sparsely bristly; culm-sheaths truncate at apex and broader than sheath-blades, without transverse veinlets or inconspicuous; leaf-blades small, 1.5-2.5 cm broad, without visible transverse veinlets .................................................... 6. A. venusta 4. Culm-sheaths with auricles and oral setae, slightly farinose, densely bristle at base; leaf-blades rather narrow, 0.8-1.8 cm broad ............ 3. A. chienouensis 3. Young culms entirely glabrous; leaf-blades rather narrow, 1.2-1.8 cm broad ....................................................................................... 2. A. brilletill     

竹亚科刚竹属植物的修订(Ⅲ)

作为对刚竹属 (Phyllostachys Sieb.et Zucc.)一些类群加以评注的第三部分 ,讨论了金竹 (Ph.sul-phurea(Carr.) A.et C.Riv.)在中国的分布 ,明确了它的具体产地 ,确认了它在华东皖浙赣毗邻地区仍然存在着一定数量的野生居群 ;同时还对本种种下变异类型重新作了分类学处理 ,承认其下包括 1变种 3变型 ,作出 1个新组合 ,归并了 2个不可靠的变型的拉丁学名和 5个命名上多余的拉丁学名。     

Taxonomic notes on Metasasa and Indocalamus nanunicus (Poaceae: Bambusoideae)

Based on comprehensive field observations and careful herbarium studies, we discuss the status of the type species of Metasasa W. T. Lin (M. carinata W. T. Lin) and entities previously treated as synonyms of its name. Three species in two genera were involved. First, M. carinata and M. albofarinosa W. T. Lin were confirmed to be conspecific and a new combination Acidosasa carinata (Lin) D. Z. Li & Y. X. Zhang is proposed. Secondly, although M. carinata was previously treated as a synonym of Acidosasa nanunica (McClure) C. S. Chao & G. Y. Yang, close morphological study revealed that these two are not conspecific. Pseudosasa nanunica (McClure) Z. P. Wang & G. H. Ye is restored to accommodate this distinct species. Finally, another name previously treated as a synonym of A. nanunica, Pseudosasa acutivagina T. H. Wen & S. C. Chen should also be resumed. Synonyms are provided for each of these three species.     

云南及邻近地区竹亚科增补

报道了云南竹亚科新记录２种,即冷箭竹和西藏香竹,归并了５个种名,它们是Ｃｈｉｍｏｎｏｃａｌａｍｕｓ ｔｏｒｔｕｏｓｕｓ Ｈｓｕｅｈ ｅｔ Ｙｉ,Ｄｅｎｄｒｏｃａｌａｍｕｓ ｆａｃｔｉｔｕｓ Ｙｉ,Ｃｈｉｍｏｎｏｂａｍｂｕｓａ ｙｕｎｎｎｅｎｓｉｓ Ｈｓｕｅｈ ｅｔ Ｗ．ｐ．Ｚｈａｎｇ,Ｃｈｉｍｏｎｏｂａｍｂｕｓａ ｎｅｐｏｕｒｐｕｒｅａ Ｙｉ,Ｆｅｒｒｏｃａｌａｍｕｓ ｒｉｍｏｓｉｖａｇｉｎｕｓ     

A Study on the Genus Gynostemma Bl. (Cucurbitaceae) from China

The genus Gynostemma B1. consists of 13 species and 2 varieties in the whole world, among which 11 species and 2 varieties occur in China. They are distributed in S. Shaanxi and the southern part of the Yangtze River (including Taiwan province) in China and also in Korea, Japan, Sri Lanka, India and Malesia. Based on the characters and dehiscence of fruit, the genus Gynostemma B1. may be divided into two subgenera, i.e. Subgen. I. Gynostemma and Subgen. II. Trirostllum (Z. P. Wang et Q. Z. Xie) C. Y. Wu ct S. K. Chen, comb. nov. 1. Subgenus Gynostemma. The fruits are baccate, globose, 3-umbonate and incorni culate on the apical side, indehiscent when mature. The style apex in female flower is bifid. Type of subgenus: Gynostemma pentaphyllum (Thunb.) Mak. This subgenus contains 8 species and 2 varieties in the world, among which 6 species and 2 varieties occur in China, i.e.1.G. simplicifolium B1. (Yunnan, Hainan of Guangdong); 2. G. laxum (Wall.) Cogn. (S. Yunnan, Hainan of Guangdong and Guangxi); 3. G. burmanicum King ex Chakr. (Yunnan), 3a. G. burmanicum var. molle C. Y. Wu (Yunnan); 4. G. pentaphyllum (Thunb.) Mak. (S. Shaanxi and the soutern area of the Yangtze River of China), 4a. G. pentaphyllum (Thunb.) Mak. var. dasycarpum C. V. Wu (Yunnan); 5. G. pubescens (Gagnep.) C. Y. Wu, st. nov. (Yunnan); 6. G. longipes C. Y. Wu, sp. nov. (endemic to China: Yunnan, Sichuan, Guizhou, Shaanxi and Guangxi). 2. Subgenus Trirostellum (Z. P. Wang et Q. Z. Xie) C. Y. Wu et S. K. Chen, comb. nov.——Trirostellum Z. P. Wang et Q. Z. Xie in Acta Phytotaxonomia Sinica 19 (4): 483. 1981, syn. nov. The fruit are capsules, subcampanulate, 3-corniculate on the apical side, dehiscent when mature. The style apex in female flower is luniform and irregularly denticulate at margin, rarely bifid. Type of subgenus: Gynostemma cardiospermum Cogn. ex Oliv. This subgenus comprises 5 species, which are all endemic to China. 1. G. yixingense (Z. P. Wang et Q. Z. Xie) C. Y. Wu et S. K. Chen (Jiangsu and Zhejiang); 2. G. cardio spermum Cogn. ex Oliv. (Hubei, Shaanxi and Sichuan); 3. G. microspermum C. Y. Wu et S. K. Chen (S. Yunnan); 4. G. aggregatum C. Y. Wu et S. K. Chen (NW. Yunnan); 5. G.laxiflorum C. Y. Wu et S. K. Chen (Anhui).