共查询到20条相似文献,搜索用时 24 毫秒
1.
Eman M. Alissa Suhad M. Bahjri Waqar H. Ahmed Nabeel Al-ama Gordon A.A. Ferns 《Journal of trace elements in medicine and biology》2006,20(2):105-114
BACKGROUND: Traditional coronary risk factors do not fully explain variations in the incidence of cardiovascular disease (CVD). Epidemiological studies have implicated perturbations in selenium, copper, and zinc metabolism in the aetiology of CVD. However, these studies have been principally undertaken in Caucasian populations, in whom trace element intake is generally sufficient. METHOD: We have measured serum and urine selenium, copper, and zinc; and superoxide dismutase, glutathione peroxidase, and lipid peroxide concentrations in 130 Saudi male subjects with established CVD, and 130 age-matched controls. RESULTS: Diabetes mellitus, positive smoking habit (p<0.0001 for both), and hypertension (p<0.05) were more prevalent among CVD patients. Urinary copper (p<0.0001) and zinc (p<0.05) were higher among controls. Serum selenium concentrations were lower among CVD patients (p<0.001), and a high proportion (52%) had selenium levels below 79mug/L compared to controls (22%) (p<0.0001). Conditional logistic regression analysis, showed the characteristics differentiating CVD patients from controls were serum zinc (odds ratio (OR) 0.92, confidence interval (CI) 0.85-0.99, p<0.05), serum copper/zinc ratio (OR 0.31, CI 0.10-0.96), serum selenium (OR 0.07, CI 0.02-0.31, p<0.0001), and urine selenium (OR 3.34, CI 1.40-7.99, p<0.01). CONCLUSION: Measures of trace metals status appear to be associated with the risk of atherosclerosis in a Saudi male population. 相似文献
2.
Ayhan Dogukan Nurhan Sahin Mehmet Tuzcu Vijaya Juturu Cemal Orhan Muhittin Onderci James Komorowski Kazim Sahin 《Biological trace element research》2009,131(2):124-132
The present study was conducted to investigate the effects of chromium histidinate (CrHis) against experimentally induced
type II diabetes and on chromium (Cr), zinc (Zn), selenium (Se), manganese (Mn), iron (Fe), and copper (Cu) in serum, liver,
and kidney of diabetic rats. The male Wistar rats (n = 60, 8 weeks old) were divided into four groups. Group I received a standard diet (12% of calories as fat); group II were
fed standard diet and received CrHis (110 mcg CrHis/kg body weight per day); group III received a high-fat diet (HFD; 40% of calories as fat) for 2 weeks and then
were injected with streptozotocin (STZ) on day 14 (STZ, 40 mg/kg i.p.; HFD/STZ); group IV were treated as group III (HFD/STZ)
but supplemented with 110 mcg CrHis/kg body weight per day. The mineral concentrations in the serum and tissue were determined
by atomic absorption spectrometry. Compared to the HFD/STZ group, CrHis significantly increased body weight and reduced blood
glucose in diabetic rats (p < 0.001). Concentrations of Cr, Zn, Se, and Mn in serum, liver, and kidney of the diabetic rats were significantly lower
than in the control rats (p < 0.0001). In contrast, higher Fe and Cu levels were found in serum and tissues from diabetic versus the non-diabetic rats
(p < 0.001). Chromium histidinate supplementation increased serum, liver, and kidney concentrations of Cr and Zn both in diabetic
and non-diabetic rats (p < 0.001). Chromium supplementation increased Mn and Se levels in diabetic rats (p < 0.001); however, it decreased Cu levels in STZ-treated group (p < 0.001). Chromium histidinate supplementation did not affect Fe levels in both groups (p > 0.05). The results of the present study conclude that supplementing Cr to the diet of diabetic rats influences serum and
tissue Cr, Zn, Se, Mn, and Cu concentrations. 相似文献
3.
Several studies have observed an inverse association between serum magnesium and fasting glucose levels. However, there have
been sparse data on Koreans. The present study was designed to evaluate the association between serum magnesium and fasting
serum glucose levels in Korean adults. A total of 949 Korean adults who visited an outpatient at a university hospital were
included in this study. We compared mean values of clinical data according to the tertile of serum magnesium level using analysis
of covariance. Association between serum magnesium and fasting glucose levels was evaluated using multiple regression analysis.
Odds ratio (OR) was calculated to determine the association between hypomagnesemia and abnormal glucose metabolism. Fasting
serum glucose levels decreased significantly from the lowest to the highest tertile of serum magnesium level (108.0 ± 1.4,
106.5 ± 2.1, 102.5 ± 1.7 mg/dl, respectively, p = 0.015), whereas blood pressure and lipid profile showed no significant associations. Serum magnesium level was negatively
associated with fasting serum glucose level (β = −0.114, p = 0.001). Participants with hypomagnesemia had significantly higher OR for abnormal glucose metabolism compared to those
with normomagnesaemia (OR = 2.28, 95%CI 1.29–4.02). There was a negative association between serum magnesium and fasting glucose
levels in Korean adults. In addition, hypomagnesemia was associated with abnormal glucose metabolism. 相似文献
4.
Dayangku Fatiha Pengiran Burut Anwar Borai Callum Livingstone Gordon Ferns 《Cell stress & chaperones》2010,15(4):379-386
Heat shock protein 27 (Hsp27) is over-expressed when cells are exposed to stressful conditions that include oxidative stress.
Oxidative stress has been implicated in the pathogenesis of cardiovascular disease (CVD), diabetes and insulin resistance.
We have investigated the concentrations of serum Hsp27 antigen and antibodies in subjects from different glycaemic categories,
who either did or did not have established CVD. Serum Hsp27 antigen and antibody levels (immunoglobulins M and G (IgM and
IgG)) were determined by enzyme-linked immunosorbent assays (ELISAs) in 68 individuals: 26 with normal glucose tolerance (NGT),
10 with (+) and 16 without (−) a history of CVD and 42 individuals with varying degrees of glucose intolerance (GI; 21 with
and 21 without a history of CVD). Insulin sensitivity was determined in each subject using indices derived from the homeostasis
model assessment of sensitivity and the insulin sensitivity index for glycaemia. Serum Hsp27 concentrations were significantly
higher in GI (+CVD) subjects compared to GI (−CVD) subjects (p = 0.03), NGT (−CVD) subjects (p = 0.02) and NGT (+CVD) subjects (p = 0.04) and were positively correlated to fasting plasma glucose for all subjects (r = 0.28, p = 0.03). IgM antibody levels were significantly higher in GI (+CVD) subjects compared to NGT (−CVD) group (p = 0.02) and were inversely related to fasting insulin concentrations (r = −0.27, p = 0.04) and the 2-h insulin concentrations (r = −0.29, p = 0.03) for all subjects. Serum IgG antibody levels were higher in GI (+CVD) group compared to GI (−CVD) group (p = 0.06). In conclusion, Hsp27 and its antibody concentrations appear to relate to the presence of cardiovascular complications
in patients with GI. 相似文献
5.
The Protective and Antidotal Effects of Taurine on Hexavalent Chromium-Induced Oxidative Stress in Mice Liver Tissue 总被引:1,自引:0,他引:1
Acute exposure to hexavalent chromium [Cr(VI)] compounds can cause hepatotoxicity. Reactive intermediates and free radicals
generated during reduction process may be responsible for Cr(VI) toxicity. In this study, the effects of pretreatment or posttreatment
of taurine on Cr(VI)-induced oxidative stress and chromium accumulation in liver tissue of Swiss Albino mice were investigated.
Single intraperitoneal (ip) potassium dichromate treatment (20 mgCr/kg), as Cr(VI) compound, significantly elevated the level
of lipid peroxidation as compared with control group (p < 0.05). This was accompanied by significant decreases in nonprotein sulfhydryls (NPSHs) level, superoxide dismutase (SOD),
and catalase (CAT) enzyme activities as well as a significant chromium accumulation in the tissue (p < 0.05). Taurine administration (1 g/kg, ip) before or after Cr(VI) exposure resulted in reduction of lipid peroxidation
(p < 0.05) showed rebalancing effect on tissue NPSH levels either in pretreatment or in posttreatment (p < 0.05). Enzyme activities of SOD and CAT were restored by taurine pretreatment (p < 0.05), whereas posttreatment had less pronounced effects on these parameters. On the other hand, taurine treatment, before
or after exposure, could exert only slight decreases in tissue Cr levels (p > 0.05). In view of the results, taurine seems to exert some beneficial effects against Cr(VI)-induced oxidative stress in
liver tissue. 相似文献
6.
Krepuska M Szeberin Z Sótonyi P Sarkadi H Fehérvári M Apor A Rimely E Prohászka Z Acsády G 《Cell stress & chaperones》2011,16(3):257-265
It has been previously reported that serum levels of 70-kDa heat shock protein (Hsp70) are elevated in peripheral artery disease.
The aim of the present study was to examine whether increased serum Hsp70 levels are related to the extent of arterial calcification
and standard laboratory parameters of patients with peripheral artery disease, as well as to markers of inflammation (C-reactive
protein), atherosclerosis (homocysteine), and calcification (fetuin-a). One hundred eighty chronic atherosclerotic patients
with significant carotid stenosis and/or lower extremity vascular disease were enrolled in this cross-sectional study. Systemic
atherosclerosis and calcification was assessed by ultrasound (carotid intima–media thickness (IMT), presence of calcification
at the abdominal aorta, carotid and femoral bifurcations, and aortic and mitral cardiac valves). Standard serum markers of
inflammation, diabetes, renal function, ankle-brachial indexes, and traditional risk factors for atherosclerosis were noted.
Serum Hsp70 levels were measured with enzyme-linked immunosorbent assay. Standard laboratory parameters (clinical chemistry),
C-reactive protein (CRP), and homocysteine levels were determined by an autoanalyzer using the manufacturer’s kits. Fetuin-a
levels were measured by radial immunodiffusion. Patients’ median age was 64 (57–71) years, 69% were men, and 34.5% had diabetes.
Serum heat shock protein 70 levels were significantly higher in patients with more severe arterial calcification (p < 0.02) and showed significant positive correlations with serum bilirubin (r = 0.23, p = 0.002) and homocysteine levels (r = 0.18, p = 0.02). Serum Hsp70 did not correlate with body mass index, IMT, CRP, or fetuin-a levels in this cohort. Logistic regression
analysis confirmed the association between sHsp70 and calcification score (OR, 2.189; CI, 1.156–4.144, p = 0.016) and this correlation remained significant (OR, 2.264; CI, 1.021–5.020, p = 0.044) after the adjustment for age, sex, eGFR, smoking, CRP, and homocysteine levels. Our data show that serum Hsp70 levels
correlate with the severity of atherosclerosis in patients with carotid artery disease and chronic lower limb ischemia. These
data support a putative role for plasma Hsp70 in the development of arterial calcification. Nevertheless, further studies
are required to investigate the usefulness of circulating Hsp70 level as a marker of atherosclerotic calcification. 相似文献
7.
Ghazi Sh Habibian M Moeini MM Abdolmohammadi AR 《Biological trace element research》2012,146(3):309-317
This experiment was carried out to investigate the effects of different levels of organic and inorganic chromium on the performance,
immune function and some serum mineral concentrations of broilers under heat stress condition (23.9–37°C cycling). A total
of 150 one-day-old broiler chicks according to a completely randomized design were assigned into five treatment groups. Each
treatment consisted of three replicates and each replicate contained ten chicks. Chicks were fed on corn–soybean meal basal
diets with added different concentrations of chromium (0, 600 and 1,200 μg kg−1 chromium chloride or 600 and 1,200 μg/kg chromium l-methionine) from 1 to 49 days of age. Humoral immunity was assessed by intravenous injection of 7% sheep red blood cell (SRBC)
followed by evaluation of serum for antibody titers in primary and secondary responses. Cell-mediated immunity was assessed
by the cutaneous basophil hypersensitivity (CBH) test to phytohemagglutinin (PHA)-P at day 32 and PHA-M at day 48. Heterophil/lymphocyte
(H/L) ratio was also measured as a reliable indicator of stress. The body mass, feed intake and conversion ratio were not
influenced by dietary chromium (P > 0.05). Dietary supplementation of both organic and inorganic chromium significantly increased primary and secondary antibody
responses (P < 0.01), and also improved H/L ratio (P < 0.05), CBH response (P < 0.01) as well as relative weights of thymus (P < 0.05) and spleen (P < 0.01). Both dietary organic and inorganic chromium caused an increase in serum concentrations of Cr and Zn (P < 0.01), but decreased the serum concentration of Cu (P < 0.01). These results suggest that supplemental chromium especially in organic form offers a good management practice to reduce
heat stress-related depression in immunocompetence of broiler chicks. 相似文献
8.
Imamoğlu N Uyanik F Kocaoğlu Güçlü B Erdem O Cem Liman B Dönmez Altuntaş H 《Biological trace element research》2008,125(2):133-140
We report the effects of chromium picolinate (CrPic) on micronucleus frequency, morphology of lymphocytes, and lipid peroxidation
in calves. Twenty-four Holstein calves were selected for the study. They were kept in a farm and were fed a commercially available
calf diet and alfalfa, ad libitum. The animals were divided into three groups of eight subjects each and were treated as follows:
The first group was supplemented with a daily dose of 200 μg Cr as chromium picolinate; a second group received 400 μg Cr
per day and a third group that served as control received no supplemental chromium. After 12-week supplementation, blood samples
were collected to determine the micronucleus frequency, the apoptotic cell percentage, and the malondialdehyde (MDA) and blood
chromium levels. In both supplemented groups, the cells had irregularly shaped and segmented nuclei. Supplementation also
increased the percentage of apoptotic cells (p < 0.001) and serum MDA (p < 0.01) and slightly increased the chromium levels. The animals supplemented with 400 μg showed a significant increase of
micronucleus frequency (p < 0.01). The results of this study suggest that supplementation with 200 and 400 μg chromium as chromium picolinate may lead
to cytotoxicity. The higher level of supplementation may also have genotoxic effects. However, further studies investigating
the mechanism of the action of CrPic are required. 相似文献
9.
Ozkaya M Sahin M Cakal E Gisi K Bilge F Kilinc M 《Biological trace element research》2009,128(2):144-151
The present study was conducted to evaluate the serum selenium levels in first-degree relatives of diabetic patients (FDR)
according to controls. Insulin resistance, serum lipid levels, inflammation markers, and blood pressure were also studied
in these patients. Serum levels of selenium in FDR were significantly lower than control group (74.65 ± 5.9 vs 88.7 ± 8.7 μg/dl,
p < 0.0001). HsCRP, HOMA-IR, insulin, homocysteine levels were significantly higher in FDR according to the control group (1.32 ± 0.9
vs 0.63 ± 0.4 mg/dL, p < 0.0001; 2.07 ± 0.84 vs 1.51 ± 0.69, p < 0.0001; 9.26 ± 3.8 vs 6.8 ± 2.98 μU/MI, p < 0.0001; 15.7 ± 7.4 vs 11.5 ± 5.1 μmol/L, p < 0.0001, respectively). There was significant correlation between selenium levels and hsCRP (r = − 0.450, p < 0.0001). There was also weak significant correlation also between HOMA-IR and selenium levels (r = −0.227, p = 0.003). There was a correlation between systolic blood pressure and BMI (r = 0.365, p < 0.0001). But there was no correlation between selenium levels and blood pressure or other parameters. HsCRP, HOMA-IR, homocysteine
levels in individuals with selenium levels < 80 μg/L (n = 78) was significantly higher than hsCRP HOMA-IR, homocysteine levels in individuals with selenium levels ≥ 80 (n = 91; 1.23 ± 0.98 vs 0.81 ± 0.76 mg/dL, p < 0.003; 1.99 ± 0.88 vs 1.64 ± 0.74, p < 0.005; 15.0 ± 7.6 vs 12.9 ± 5.7 μmol/L, p < 0.049, respectively). Selenium deficiency may contribute to cardiovascular disease risk in FDR. 相似文献
10.
It is thought that calcium and magnesium may be related to metabolic disorders such as obesity and metabolic syndrome; however,
to date, there have been few studies investigating the association between serum calcium and magnesium levels and metabolic
syndrome in middle-aged male adults. We aimed to investigate the association between serum calcium and magnesium levels and
metabolic syndrome in Korean middle-aged male adults. Study subjects included 213 men aged 30∼60 years. MetS risk score is
determined by adding the number of risk factors, waist circumference, triacylglyceride (TG), HDL cholesterol, glucose, and
blood pressure (BP). The study population was divided into three groups according to the MetS risk score: group I (MetS risk
score ≤1; n = 106), group II (MetS risk score = 2; n = 51), and group III (MetS risk score ≥3; n = 56). The serum Ca, according to increase of MetS risk score, was significantly higher (p < 0.001), and there was no significant difference in serum Mg concentration among the three groups. Subjects with high TG
and high BP had higher serum calcium levels than those without such abnormalities. Subjects with higher glucose had lower
serum magnesium levels than those without such abnormality. The correlation analysis indicated that the serum Ca had positive
correlations with the MetS risk score (r = 0.1769, p < 0.01), serum TG (r = 0.2516, p < 0.001), and DBP (r = 0.2246, p < 0.01). The correlation analysis indicated that the serum Mg had an inverse relationship with serum glucose (r = −0.2404, p < 0.001). In conclusion, serum Ca had positive association with TG and BP, while serum Mg had negative association with serum
glucose after adjusting age and BMI among the middle-aged Korean male adults. 相似文献
11.
Chintana Phawong Collins Ouma Piyatida Tangteerawatana Jarinee Thongshoob Tom Were Yuvadee Mahakunkijcharoen Duangrurdee Wattanasirichaigoon Douglas Jay Perkins Srisin Khusmith 《Immunogenetics》2010,62(6):345-356
Polymorphic variability in immune response genes, such as IL12B, encoding the IL-12p40 subunit is associated with susceptibility to severe malaria in African populations. Since the role
of genetic variation in conditioning severe malaria in Thai adults is largely unexplored, the functional association between
IL12B polymorphisms [i.e. IL12Bpro (rs17860508) and IL12B 3′ UTR T/G (rs3212227)], severe malaria and cytokine production was examined in patients with Plasmodium falciparum infections (n = 355) recruited from malaria endemic areas along the Thai–Myanmar border in northwest Thailand. Circulating IL-12p40 (p = 0.049) and IFN-γ (p = 0.051) were elevated in patients with severe malaria, while only IL-12p40 was significantly higher in severe malaria patients
with hyperparasitaemia (p = 0.046). Carriage of the IL12Bpro1.1 genotype was associated with enhanced severity of malaria (OR, 2.34; 95% CI, 0.94–5.81; p = 0.066) and hyperparasitaemia (OR, 3.42; 95% CI, 1.17–9.87; p = 0.025) relative to the IL12Bpro2.2 genotype (wild type). Individuals with the IL12Bpro1.1 genotype also had the lowest IL-12p40 (p = 0.002) and the highest IFN-γ (p = 0.004) levels. Construction of haplotypes revealed that carriage of the IL12Bpro-2/3′ UTR-T haplotype was associated with protection against severe malaria (OR, 0.51; 95% CI, 0.29–0.90; p = 0.020) and reduced circulating IFN-γ (p = 0.06). Thus, genotypic and haplotypic variation at IL12Bpro and IL12B 3′ UTR in this population influences susceptibility to severe malaria and functional changes in circulating IL-12p40 and
IFN-γ levels. Results presented here suggest that protection against severe malaria in Thai adults is associated with genotypic
variants that condition enhanced IL-12p40 and reduced IFN-γ levels. 相似文献
12.
The effect of fermented mushroom of Coprinus comatus rich in trace elements, including vanadium, chromium, zinc, magnesium, copper, iron, and nickel, on glycemic metabolism was
studied in this paper. Alloxan-induced hyperglycemic mice were used in the study. The blood glucose, glycohemoglobin, and
glycogen synthesis of the mice were analyzed, respectively. At the same time, the gluconeogenesis of the normal mice was also
determined. After the mice were administered (ig) with C. comatus rich in vanadium (CCRV), the blood glucose and the glycohemoglobin of alloxan-induced hyperglycemic mice decreased (p < 0.05, p < 0.01), glycogen synthesis of alloxan-induced hyperglycemic mice elevated (p < 0.01), the gluconeogenesis of the normal mice was inhibited (p < 0.01), and the sugar tolerance of the normal mice was improved. However, the same result did not occur in other groups.
Vanadium at lower doses in combination with C. comatus induced significant effect on glycemic metabolism in mice. 相似文献
13.
The purpose of this research was to use inline real-time near-infrared (NIR) to measure the moisture content of granules manufactured
using a commercial production scale continuous twin-screw granulator fluid-bed dryer milling process. A central composite
response surface statistical design was used to study the effect of inlet air temperature and dew point on granule moisture
content. The NIR moisture content was compared to Karl Fischer (KF) and loss on drying (LOD) moisture determinations. Using
multivariate analysis, the data showed a statistically significant correlation between the conventional methods and NIR. The
R
2 values for predicted moisture content by NIR versus KF and predicted moisture values by NIR versus LOD were 0.94 (p < 0.00001) and 0.85 (p < 0.0002), respectively. The adjusted R
2 for KF versus LOD correlation was 0.85 (p < 0.0001). Analysis of the response surface design data showed that inlet air temperature over a range of 35–55°C had a significant
linear impact on granule moisture content as measured by predicted NIR (adjusted R
2 = 0.84, p < 0.02), KF (adjusted R
2 = 0.91, p < 0.0001), and LOD (adjusted R
2 = 0.85, p < 0.0006). The inlet air dew point range of 10–20°C did not have a significant impact on any of the moisture measurements. 相似文献
14.
Esposito P Tinelli C Libetta C Gabanti E Rampino T Dal Canton A 《Cell stress & chaperones》2011,16(2):219-224
Autoimmunity to heat shock protein 60 (HSP60) has been related to atherosclerosis. Chlamydia pneumoniae (CP), the most studied infectious agent implicated in promoting atherosclerosis, produces a form of HSP60, which can induce
an autoimmune response, due to high antigenic homology with human HSP60 (hHSP60). In this study, we evaluated the correlations
among anti-hHSP60 antibodies, CP infection, and cardiovascular disease (CVD) in a high-risk population, such as patients undergoing
hemodialysis (HD). Thirty-two patients (67.9 ± 13.9 years; male/female, 23:9) on regular HD were enrolled. Global absolute
cardiovascular risk (GCR) was assessed using the Italian CUORE Project’s risk charts, which evaluate age, gender, smoking
habits, diabetes, systolic blood pressure, and serum cholesterol. The occurrence of cardiovascular events during a 24-month
follow-up was recorded. Seropositivity to CP and the presence of anti-hHSP60 antibodies were tested by specific enzyme-linked
immunosorbent assays. Inflammation was assessed by measurement of C-reactive protein (CRP) serum levels. Fifteen healthy sex
and age-matched (61.9 ± 9.5 years; male/female, 11:4) subjects were the control group. Fifteen of 32 patients resulted seropositive
for CP. CP + patients were older than CP−, while they did not differ for GCR, CRP, and dialytic parameters. CVD incidence
was significantly higher in CP+ (9 CP+ vs 2 CP−, p < 0.05). Cox analysis recognized that the incidence of CVD was independently correlated with seropositivity to CP (HR, 7.59;
p = 0.01; 95% CI = 1.63–35.4). On the other hand, there were no significant differences in anti-hHSP60 levels among CP+, CP−
and healthy subjects: 18.11 μg/mL (14.8–47.8), 31.4 μg/mL (23.2–75.3), and 24.72 μg/mL (17.7–41.1), respectively. Anti-hHSP60
did not correlate to GCR, CRP, and incidence of CVD. In conclusion, our data suggest that anti-hHSP60 autoimmune response
is not related to CP infection and CP-related CVD risk in HD patients. 相似文献
15.
Sudipto Haldar Souvik Mondal Saikat Samanta Tapan Kumar Ghosh 《Biological trace element research》2009,131(2):110-123
The effects of supplemental chromium (Cr) as chromic chloride hexahydrate in incremental dose levels (0, 0.5, 1.0, and 1.5 mg/day
for 240 days) on metabolism of nutrients and trace elements were determined in dwarf Bengal goats (Capra hircus, castrated males, average age 3 months, n = 24, initial mean body weight 6.4 ± 0.22 kg). Live weight increased linearly (p < 0.05) with the level of supplemental Cr. Organic matter and crude protein digestibility, intake of total digestible nutrients,
and retention of N (g/g N intake) increased (p < 0.05) in a dose-dependent linear manner. Serum cholesterol and tryacylglycerol concentrations changed inversely with the
dose of supplemental Cr (p < 0.01). Supplemental Cr positively influenced retention of copper and iron (p < 0.05) causing linear increase (p < 0.01) in their serum concentrations. It was concluded that Cr supplementation may improve utilization of nutrients including
the trace elements and may also elicit a hypolidemic effect in goats. However, further study with regards to optimization
of dose is warranted. 相似文献
16.
Toghyani M Toghyani M Shivazad M Gheisari A Bahadoran R 《Biological trace element research》2012,146(2):171-180
This study was conducted to determine the effects of dietary supplementation with Cr nicotinate and Cr chloride and their
optimum inclusion rate on performance, carcass traits, meat oxidative stability, serum metabolites, hematological parameters,
and liver chromium concentration in heat-stressed broilers. A total number of 420, 1-day-old male broiler chicks were randomly
assigned to seven treatments with four replicates of 15 chicks. The dietary treatments consisted of the basal diet supplemented
with 0 (control), 500, 1,000, and 1,500 μg/kg Cr in the form of Cr nicotinate and Cr chloride. Chicks were raised for 6 weeks
in heat stress condition (33 ± 2°C). Supplements of organic and inorganic Cr particularly at 1,500 μg/kg incorporation increased
feed consumption (P < 0.05) and body mass gain of broilers (P < 0.01). Cr supplementation increased carcass yield and decreased abdominal fat (P < 0.01). Supplementation of 1,500 μg/kg Cr nicotinate (P < 0.05) enhanced liver Cr concentration. Storage time increased lipid oxidation of meat (P < 0.01). Cr decreased lipid oxidation of breast and thigh muscles over 2 (P < 0.01) or 6 (P < 0.05) days of storage time. Birds fed 1,500 μg/kg Cr nicotinate, had lower concentration of serum glucose and triglyceride
at 21 days (P < 0.05). Hematological parameters tested at 21 and 42 days, were not influenced. The results suggested that dietary Cr supplementation
regardless of its source have a positive effect on productive, and carcass traits, also enhances oxidative stability of refrigerated
meat in broilers reared under heat stress conditions. 相似文献
17.
Antibodies have different avidities that can be evaluated using modified enzyme-linked immunosorbent assay (ELISA) techniques. We determined levels and avidities of antibodies to light (NFL) and medium (NFM) subunits of neurofilaments and tau protein in serum and cerebrospinal fluid (CSF) from 26 patients and anti-tau antibody levels and their avidities in 20 multiple sclerosis (MS) patients and 20 age- and sex-matched controls. Each sample was analyzed using both standard ELISA and also using a similar ELISA protocol with the addition of urea. The avidities of anti-neurocytoskeletal antibodies were higher in the CSF than those in serum (anti-NFL, p < 0.0001; anti-tau, p < 0.01; anti-NFM, n.s.). There was no relationship between avidities in serum and CSF for individual anti-neurocytoskeletal antibodies. We did not observe the relationship among the avidities of various anti-neurocytoskeletal antibodies. The avidities of anti-tau antibodies in the CSF were significantly higher in the MS patients than those in the controls (p < 0.0001). The study demonstrates the differences in avidities of CSF or serum neurocytoskeletal antibodies measured as the urea resistance by ELISA method. Avidity determination of anti-neurocytoskeletal antibodies could contribute to the evaluation of the immunological status of patients. 相似文献
18.
Suzuki K Higuchi H Iwano H Lakritz J Sera K Koiwa M Taguchi K 《Biological trace element research》2012,145(2):166-171
The aim of this study was to evaluate the reliability and effectiveness of direct determination of trace and major element
concentrations in bronchoalveolar lavage fluid samples from Holstein calves with Mycoplasma bronchopneumonia (n = 21) and healthy controls (n = 20). The samples were obtained during bronchoscopy using a standard examination method. A total of 18 elements (aluminum,
bromine, calcium, chlorine, chromium, copper, iron, potassium, magnesium, manganese, molybdenum, nickel, phosphorous, sulfur,
silicon, strontium, titanium, and zinc) were detected by particle-induced X-ray emission. The average bromine, iron, potassium,
magnesium, and phosphorous concentrations were higher in calves with bronchopneumonia than in controls (p < 0.05). They were found to have higher amounts of calcium and zinc, and a higher zinc–copper ratio than that in healthy
calves (p < 0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for zinc–copper ratio
for identification of Mycoplasma pneumonia of 8.676. Our results indicate that assessment of the elemental composition of broncholaveolar lavage fluid is
a promising diagnostic tool for Mycoplasma bronchopneumonia. 相似文献
19.
Sara Persson Britt-Marie Bäcklin Hans Kindahl Björn Brunström Ulf Magnusson 《European Journal of Wildlife Research》2011,57(5):1057-1063
The objectives of this study on the reproductive system of wild male mink (Neovison vison) were to determine the influence of age, nutritional status and season on reproductive traits and to produce baseline data
that may be used in environmental monitoring programmes. The 117 male mink included in the study were killed by hunters all
over Sweden (from latitude 56° N to 67° N) during August to the end of April from 2005 to 2008. The weights of penis, testes,
and epididymides were lower, the anogenital distance was shorter and the diameter of seminiferous tubules was less in juveniles
compared with adult mink (p < 0.0001–p = 0.0024). There was a positive effect (p < 0.05) of nutritional status on the baculum length and on the anogenital distance, but not on any of the other reproductive
traits. The season of sampling influenced the penis, testes and epididymes weights and the diameter of seminiferous tubules
(p = 0.008–p < 0.0001). During spring, 86% of the mink had sperm in their epididymides, whereas the corresponding figure for autumn was
3%; in addition, when the average diameter of the tubules reached 138 μm, sperm were present in the epididymides. Sperm morphology
was analysed in 29 of the mink, and the percentage of morphologically defect sperm were generally low. Taken together, this
study suggests that when assessing male reproductive data in wild mink populations, season must be taken into account as well
as whether the individuals are juvenile or adult. In contrast, the nutritional status seems not to be of any major significance. 相似文献
20.
Reza Amani Somaye Saeidi Zahra Nazari Sorour Nematpour 《Biological trace element research》2010,137(2):150-158
It has been suggested that mood disorders and depressive status may be accompanied by lowered zinc status in the body, and
adequate consumption of zinc increases a general perceived well-being. The main objective of this study was to assess the
correlation between serum zinc concentrations and dietary zinc intakes with depression scores in university female students.
In the first phase, Beck's depression questionnaire was applied in a random sampling of 308 selected 20–25-year-old female
students (one third of total students in Ahvaz Jondi-Shapour University of Medical Sciences Golestan dormitories) to assess
the major depressive disorder (MDD) scales. Then, in the second phase, 23 students who identified as having moderate and severe
depression were selected as the case group, and 23 healthy age matched were chosen as the controls. Each of them completed
a 12-item semiquantitative food frequency questionnaire containing the main food sources of zinc in the usual dietary patterns
and also a 24-h food recall questionnaire to assure the daily zinc intakes. Daily zinc intakes were obtained by multiplying
each portion size by its zinc content using food tables. A 5-ml blood sample was taken for further serum zinc status using
flame atomic absorption spectrophotometry technique. Pearson's r was used to show the correlation between quantitative variables. Both daily zinc intake and serum zinc concentrations of
MDD group were about two thirds of healthy index (p < 0.01). Depressed individuals used to eat lower servings of red meats and chicken as the main food sources of zinc in students'
usual diets (p < 0.001). Consumption of other foods as the sources of zinc was not significantly different in two groups. A linear significant
correlation between dietary zinc intakes and its serum levels was seen in samples (r = 0.62; p < 0.001) and MDD students (r = 0.55; p < 0.001). There was a linear inverse correlation between Beck questionnaire scores and serum zinc concentrations in all of
the investigated students(r = -0.65; p < 0.001) and MDD girls (r = −0.71; p < 0.001). Beck questionnaire scores and diatary zinc intakes were also inversly correlated (r = −0.58; p < 0.001). However, no statistical correlation was seen between these two variables in MDD cases. In depressed female students,
dietary zinc intake is correlated to its serum concentrations; however, the serum zinc levels are inversely correlated to
depression scales. Consumption of the main dietary sources of zinc such as red meats and chicken should be encouraged in young
depressed girls. 相似文献