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 This paper deals with material of Pemphigidae and Lachnidae from Fujian Province, China.A new record genus Gharesia Stroyan, 1963 of Pemphigidae is described; and three new species Gharesia kolokasia Qiao et Zhang, sp.nov., Cinara jianglensis Zhang et Qiao, sp.nov.and Eulachnus drakontos Zhang et Qiao, sp.nov.are new to science.All specimens are deposited in Institute of Zoology,the Chinese Academy of Sciences.  相似文献   

3.
 Five species and one subspecies of the Coleophora directella group are reported from north China in this paper.Among them,the subspecies Coleophora algidella qinlingensis ssp.nov.and two species Coleophora estriatella sp.nov.and Coleophora heihensis sp.nov.are described as new to science,and the other two species Coleophora directella Zeller and Coleophora expressella Klemensiewicz are newly recorded from this country.  相似文献   

4.
 In this study,Scolopendra negrocapitis sp.nov.is described.It obviously differs from S.japanica L.Koch distributed over the inland of Japan,in characters of the prefemur of last legs about two times as long as broad and comparatively stout than that of the latter.Statistics show that their ventral thorns of last legs usually 3 at the outer and 2 at the inner sides,and this mode in the materials is about 90 per cent.  相似文献   

5.
 Two new genera and three new species of Cephidae are described in the paperMiscocephus cyaneus gen.et sp.nov., Megajanus longithecus gen.et sp.nov.,Caenocephus tianmunicus sp.nov..Caenocephus is recorded in China for the first time. The type specimens are deposited in Insect Collection of Central South Forestry University, Zhuzhou,Hunan Province,China.  相似文献   

6.
 In this paper,7 new species of genus Mansoniella are describedM.annulata sp.nov.,M.cristata sp.nov.,M.elongata sp.nov.,M.flava sp.nov.,M.juglandis sp.nov.,M.rosacea sp.nov.,M.rubida sp.nov.; 3 new combinations are suggestedM.cinnamomi(Zheng et Liu, 1992), comb.nov., M.sassafri(Zheng et Liu,1992), comb.nov.,M.wangi(Zheng et Li, 1992), comb.nov..The type specimens are deposited in the Department of Biology,Nankai University, Tianjin, China.  相似文献   

7.
Spencer D  White RG  Wildman SG 《Protoplasma》2005,225(3-4):185-190
Summary. Confocal laser scanning microscopy was used to study the distribution of the smallest detectable autofluorescing, chlorophyll-bearing structures in fresh, 40 μm thick longitudinal sections of the shoot apex of four dicotyledonous plants (Arabidopsis thaliana, Nicotiana glauca, Lupinus alba, and Spinacia oleracea). In all species, the smallest chlorophyll-bearing particles were found in the outermost cell layers (L1 and L2) of the shoot apex. Their distribution between these layers differed in each species. The smallest such particles were about 0.5–1.0 μm in maximum dimension, approximating the size of a single granum in the developing leaf. Their size and abundance increased with increasing cell age and distance from the peak of the apex. Immediately beneath the L1 and L2 layers was a zone largely devoid of these particles. Below this nonfluorescing zone, in the region where the derivatives of the meristematic zone differentiate into cells of the central pith region, the size and abundance of the chlorophyll-bearing particles increased progressively with increasing distance from the nonfluorescing zone. The presence of these small autofluorescing particles in the L1 and L2 cell layers of the shoot apex places the development of photosystem II fluorescence at an earlier stage of leaf development than previously observed. The use of confocal laser scanning microscopy to study unfixed sections provides another useful metabolic marker for mapping patterns of differentiation and development in the cells of the shoot apex. Correspondence and reprints: CSIRO Plant Industry, GPO Box 1600, Canberra, A.C.T. 2601, Australia.  相似文献   

8.
Summary When IPL-Sf-21AE III continuous insect cell line was grown and maintained in IPL-41 insect cell culture medium supplemented with 16μM of AlCl3 or 0.24μM of ZnSO4·7H2O, or both metallic salts, and then infected withAutographa californica nuclear polyhedrosis virus, virus replication was increased significantly. The yield of polyhedral inclusion bodies (PIB) was enhanced up to 121%. Synthesis of cellfree nonoccluded virus was increased to 365% when infectivity was assayed by the plaque method. Newly applied electron microscopic quantitation and stereological techniques also revealed a significant increase in virus particles (VP) and in amount and size of PIB as well as number of VP per PIB. Part of this program was supported by Grant 58-3204-8-5 from the United States Department of Agriculture, Science and Education Administration, Beltsville Agricultural Research Center, Beltsville, Maryland, and Grant DAR-8021956 from the National Science Foundation, Washington, D.C. We are also indebted to the Edgar G. Tobin Foundation of San Antonio, Texas, for the donation of a Royco TC-927 cell counter, to Burkitt Foundation, Houston, Texas, for the Wescor Osmometer, and Donald W. Reynolds Foundation, Las Vegas, Nevada, for the Leitz microscopy equipment. Mention of a commercial product does not constitute a recommendation by the U.S. Department of Agriculture.  相似文献   

9.
Measurement of intracellular volume in monolayers of cultured cells   总被引:1,自引:0,他引:1  
Summary Methods have been developed for measuring the intracellular water space (ICS) in cultured diploid fibroblast monolayers. The values obtained have been compared to similar measurements of ICS in suspended fibroblasts using an oil spin method. Markers commonly used for measurement of total water space (TWS) ([3H]H2O, [14C]urea) and extracellular space (ECS) ([3H]inulin, [14C]l-glucose, [3H]sucrose, [3H]d-mannitol) were investigated for use in cell monolayers. Monolayer incubations were terminated by rapidly rinsing the culture dishes three times with ice cold buffer. The distribution volume of the TWS marker [14C]urea plateaued at 10 to 15 min and was independent of urea concentration. [3H]H2O was not a suitable marker for measuring total water space in cell monolayers because of rapid loss of intracellular label during rinsing. Intracellular space was calculated by subtracting [3H]sucrose space (5 min) from [14C]urea space (20 min) after incubation of fibroblasts with both markers. Values obtained for ICS (mean ± SE;μl/106 cells) of fibroblasts from two cell lines measured in monolayer (1.74±0.11 and 1.60±0.10) and in suspension (1.88±0.07 and 1.78±0.11) was approximately 10% smaller than the values for cell size (2.01 and 2.22) obtained from Coulter Counter sizing. Thus, the methods developed for measurement of ICS in monolayer fibroblasts yield data comparable to those obtained with the more standard oil spin method. Furthermore, the methods can be applied to measurement of ICS in other types of adherent cells. This work was supported in part by funds from the National Institutes of Health Contract N01-AM-9212.  相似文献   

10.
Two six-week laboratory experiments were conducted to evaluate effects of pesticides and microcosm size on benthic estuarine macroinvertebrate recolonization. Sediments fortified with the pesticides (fenvalerate: controls, 5 (low) and 50 μg g−1 wet sediment (high); endosulfan: controls, 1 (low) and 10 μg g−1 wet sediment (high)) were fine-grained, organically rich (approximately 3.5% organic carbon and 22% dry weight) material. Relative dominance of the four most abundant taxa in both experiments was consistent among treatments with few exceptions. The amphipod,Corophium acherusicum, dominated abundance in both experiments. In the fenvalerate experiment, large trays (400 cm2) contained significantly (p<0.05) more total number of taxa (TNT) than small microcosms (144 cm2) but tray size was not significantly related to total number of organisms (TNO). When size was adjusted to a common unit area, small trays contained significantly more TNO than large containers. Adjusted abundance of small trays was 2.5 times that of large containers; a ratio close to that of microcosm sizes (i.e., 2.8). This result suggests that larval supply may have been inadequate to ‘aturate’ the available sediment in large containers. Fenvalerate significantly reduced abundance in the high treatment compared to both controls and low treatment but low treatment was not significantly different from controls. The amphipod,Corophium acherusicum, accounted for most of the decrease in abundance in response to fenvalerate. The holothruroid,Leptosynpta sp. and the polychaete,Mediomastus ambiseta, increased in abundance significantly with increased concentration of fenvalerate. Combined effects of actual microcosm size and concentration of endosulfan were not significant for TNO or TNT. As in the fenvalerate experiment, adjusted abundance of small microcosms was 2.6 times that of large trays which approximated the ratio of unit area between microcosm sizes. Abundance of a few taxa responded significantly to adjusted and unadjusted unit area. Abundance of the tunicate,Molgula manhattensis, increased significantly with increased concentration of endosulfan. Abundance was affected by sample location (e.g., interiorvs exterior cores) within microcosms. Abundance adjusted to unit area resulted in significantly greater TNO in externalvs internal cores. This has importance for sequential sub-sampling of microcosms to determine temporal dynamics. Statistically significant effects were measured in benthic community structure associated with microcosm size; however, the magnitude was relatively small. There appears to be no major biological reason to select one microcosm size over the other for screening for contaminant effects. Where feasible, the small trays provide savings in sample preparation and analysis, allow more replicates where laboratory space is limiting and generate less chemical waste. These benefits may be off-set by less ‘artifacts’ associated with edge effects of larger microcosms and the need for a larger mass of sediment to accommodate additional analytical requirements (e.g., thin vertical surficial samples to refine contaminant exposure at the sediment/water interface).  相似文献   

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