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1.
Beata Kułek Jolanta Floryszak-Wieczorek Hanna Jackowiak 《Acta Physiologiae Plantarum》2004,26(1):95-102
The involvement of β-D-glucosidase activity in grey mould was studied in two ornamental plant species attacked by Botrytis cinerea.
β-D-glucosidase activity in the susceptible pelargonium cultivar ‘Shiva’ gradually increased with the disease development
in the leaf spots and their surroundings. The endogenic level of the studied hydrolase in the resistant pelargonium ‘Cascade’
was several times higher than in the susceptible cultivar ‘Shiva’ and in principle underwent no changes after inoculation.
The postinfection increase in the activity of β-D-glucosidase noted in the leaves of the susceptible poinsettia cultivar ‘Malibu
Red’ was evidently weaker in the intensity, but its tendencies were similar to those of the susceptible pelargonium cultivar.
In the leaves of the medium-resistant poinsettia ‘Coco White’ the constitutional level of β-D-glucosidase was 2-3-fold higher
in that cultivar than in the susceptible cv. ‘Malibu Red’. In attacked leaves of ‘Coco White’, the enzyme activity continued
to increase temporarily until the 3rd h after inoculation.
The process of healthy leaf senescence in both species had no significant influence on the change of the studied enzyme activity
which was generally low. A high activity of β-D-glucosidase was also observed in the homogenate prepared from mycelium and
in the fungal spores. 相似文献
2.
Hiroaki Egashira Akira Kuwashima Hiromi Ishiguro Kazuhiro Fukushima Takashi Kaya Shigeru Imanishi 《Acta Physiologiae Plantarum》2000,22(3):324-326
Tomato gray mold (Botrytis cinerea Pers.) is a common disease worldwide, and often causes serious production loss by infecting leaves, stems, flowers and fruits.
Presently, no resistant cultivars are available. To find new breeding materials for gray mold resistance, assessment for resistance
of the leaflet and stem in six tomato cultivars, 44 wild tomato accessions and a Solanum lycopersicoides accession was performed. Although no correlation was observed (r=−0.127ns) between resistance of the leaflet and the stem, L. peruvianum LA2745, L. hirsutum LA2314 and L. pimpinellifolium LA1246 showed high resistance both in the leaflet and in the stem. Particularly, in the leaves of LA2745, no lesions were
observed even more than two weeks after the inoculation with conidia, and F1s between a cultivated tomato and LA2745 also
showed high resistance as observed in LA2745. From these results, LA2745 is thought to be a promising material for breeding
gray-mold resistant cultivars. 相似文献
3.
C. M. de Jager R. P. T. Butt P. G. L. Klinkhamer T. J. de Jong K. Wolff E. van der Meijden 《Entomologia Experimentalis et Applicata》1995,77(3):277-287
In a choice-experiment, 42 chrysanthemum cultivars were screened for resistance toFrankliniella occidentalis (Pergande). Oviposition preference, two types of feeding damage and thrips numbers per flower were recorded as measures of
resistance. A large genetic variation in thrips resistance was found among the cultivars screened. The amount of feeding damage
was strongly determined by oviposition preference. Besides, a positive correlation was found between the oviposition preference
in non-flowering chrysanthemums (number of eggs) and flowering chrysanthemums (number of thrips per flower). Thrips feeding
on young, developing tissues, causes growth damage because affected cells are unable to expand and leaves become distorted.
Thrips feeding on older, expanded leaves causes cells to become filled with air, resulting in ‘silver’ damage. The amounts
of growth-and ‘silver’ damage were negatively correlated suggesting that thrips chose either young or older leaves to feed
on. The order of resistance among cultivars did not change during the experiment. In order to get more insight in resistance
mechanisms the influence of some plant- and flower characters on resistance was examined. The plant characters height, number
of leaves, flower production and flower weight were all negatively correlated with resistance. It is suggested that tall chrysanthemum
cultivars with many and large flowers may invest less in defence than smaller cultivars, and therefore are more damaged by
thrips. 相似文献
4.
Response of twenty eight cultivars of durum wheat (Triticum turgidum var. durum) to immature embryo culture, callus production and in vitro salt tolerance was evaluated. For assessment of cultivars to salt tolerance, growing morphogenic calli were exposed to different
concentrations of NaCl (0, 0.3, 0.6, 0.9, 1.2, 1.5, 1.8 and 2.1% w/v) added to the culture medium during two subsequent subcultures
(4 weeks each). Comparison of cultivars for callus induction from immature embryo was based on callus induction frequency
and fresh weight growth of callus (FWG). While, for salt tolerance, the relative fresh weight growth (RFWG) and necrosis percent
of callus were used. There were significant differences among cultivars for potential of regeneration from immature embryo,
and ‘Shahivandi’ a native durum wheat cultivar originating from western Iran was superior among the cultivars tested. The
FWG distinguished cultivars more than callus induction frequency did for callus induction evaluation. Hence, a range of FWG
from 1.23 to 14.65 g was observed in ‘Mexical-75’ and ‘Omrabi-5’ cultivars, respectively. Growing calli derived from cultivars
‘PI 40100’ and ‘Dipper-6’ showed superiority for tolerating salinity under in vitro conditions.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Ying Li Yuping Song Gongjun Shi Jianjun Wang Xilin Hou 《Acta Physiologiae Plantarum》2009,31(1):155-162
Changes in ascorbic acid content and antioxidant enzyme activities were investigated in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) leaves of ‘Wutacai’ and ‘Erqing’ exposed to excess copper (Cu). Cu treatment reduced the fresh weight of shoot and
root by 57% and 46% in ‘Wutacai’, and 60 and 54% in ‘Erqing’, respectively. The accumulation of copper in leaves was higher
in ‘Wutacai’ than that in ‘Erqing’. Compared to the control, ascorbic acid (AsA) contents were significantly decreased after
copper treatment in both cultivars, while they were higher in ‘Wutacai’ than in ‘Erqing’, which may explain the higher copper-tolerance
of ‘Wutacai’ with higher copper accumulation. The higher AsA contents of ‘Wutacai’ resulted from their lower activities of
degrading enzymes, such as ascorbate oxydase (AAO) and ascorbate peroxidase (APX), as well as the increasing activity of dehydroascorbate
reductase (DHAR) after copper treatment compared with ‘Erqing’. Copper stimulated superoxide dismutase (SOD) activity in both
cultivars, but for catalase (CAT), there was little difference between both cultivars. Peroxidases (POD) activity was decreased
after copper treatment in ‘Erqing’, while in ‘Wutacai’, it was significantly increased at 14 days, and POD activity was higher
in ‘Wutacai’ than that in ‘Erqing’ at 21 and 28 days. Therefore, the induced increasing activity of POD in ‘Wutacai’ also
played an important role in its copper tolerance. 相似文献
6.
S. Jayasankar Richard E. Litz Raymond J. Schnell Andres-Cruz Hernandez 《In vitro cellular & developmental biology. Plant》1998,34(2):112-116
Summary Genomic DNA isolated from embryogenic cultures of two mango cultivars, ‘Hindi’ and ‘Carabao,’ that had been selected for resistance
to the culture filtrate ofColletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD).In vitro selection caused changes in RAPD markers in the selected embryogenic cultures with respect to the unchallenged control cultures
and the stock plants. The differences involved both the absence and the presence of additional RAPD markers in the resistant
lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either
cultivar and DNA from the leaves of parent trees confirmed that the changes were not due to prolonged maintenance in liquid
cultures. 相似文献
7.
Effects of exogenous B supply on growth, B accumulation and distribution of two navel orange cultivars 总被引:3,自引:0,他引:3
Ou Sheng Shang W. Song Yun J. Chen Shu A. Peng Xiu X. Deng 《Trees - Structure and Function》2009,23(1):59-68
To investigate the effects of boron (B) on growth, B concentration and distribution of two navel orange cultivars, ‘Newhall’
(Citrus sinensis Osbeck) and ‘Skagg’s Bonanza’ (Citrus sinensis Osbeck) grafted on the rootstock trifoliate orange [Poncirus trifoliata (L.) Raf.], B at five levels was exogenously supplied to 1-year-old grafted plants of both cultivars under greenhouse conditions.
Plants were grown in sand:perlite (1:1, v/v) medium and were irrigated every 2 days with half-strength Hoagland’s No. 2 nutrient
solutions containing different B, 0.01, 0.05, 0.10, 0.25 and 2.50 mg l−1 (0.25 and 2.50 mg l−1 were considered as control and excess B treatment, respectively, and the other three B levels were considered as low B treatments).
After treatments for 183 days, leaves (from basal, middle, upper parts of the shoots), stem of scion, stem of rootstock and
root were separately sampled. Our results showed that plant growth (plant height, root volume and dry weights of various parts)
was inhibited in response to low or excess B supplies in both cultivars. It was found that B concentrations in the upper leaves
of both cultivars were substantially higher than those in the basal leaves when low concentrations (≤0.05 mg l−1) of exogenous B were applied, suggesting that B was preferentially translocated to the upper-younger leaves to support their
growth. Analysis of B distribution in different parts indicated that translocation of B from the root to the scion’s shoots
(stems and leaves of scion) may be restricted upon exposure to low B conditions. When B was inadequately supplied, growth
of ‘Skagg’s Bonanza’ was better than ‘Newhall’, implying that the former cultivar was more tolerant to low B status, which
may be due to the higher efficiency of B translocation from the root to the scion’s shoots. However, when the plants were
treated with excess B (2.50 mg l−1), both cultivars showed a similar degree of B toxicity. The probability of scion–rootstock interactions in relation to the
differential responses of growth and different efficiency of B translocation involved in the two orange cultivars following
the long-term low B stress were discussed. 相似文献
8.
Legumes as ground cover are regularly planted to increase nitrogen economy of crops and to improve soil. In the present study
various clover species were evaluated as vegetative ground cover in nursery field production of micropropagated red birch
(Betula pubescensEhrh. f. rubraUlvinen f. nova) in two 2-year experiments. The clover species and cultivars, Trifolium pratenseL. ‘Bjursele’, T. repens L. ‘Jogeva’, T. repens L. ‘Sonja’, T. hybridum L. ‘Frida’, T. incarnatum L. ‘Opolska’, T. resupinatum L. and T. subterraneum L. were compared to grass sod Festuca rubra L. ‘Ensylva’ and to a coverless ground (control). The last one was kept weed free by hand hoeing. Birch (leaves, stems, branches
and roots) and soil nutrient concentrations (N, P, K, Ca, Mg and Fe) were analysed and nutrient ratios in birch determined.
The annual clovers, T. incarnatumL., T. resupinatumL. and T. subterraneumL., provided about the same nutrient status in birch as did the control. Perennial clovers and grass were strong competitors
with trees. High levels of P and Mg in birch leaves relative to N concentration were typical for poorly growing seedlings.
Neither annual nor perennial clovers did generally improve soil nutrient status.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
9.
Sibylle Stoeckli Karsten Mody Cesare Gessler Andrea Patocchi Mauro Jermini Silvia Dorn 《Tree Genetics & Genomes》2008,4(4):833-847
The rosy apple aphid (Dysaphis plantaginea), the leaf-curling aphid (Dysaphis cf. devecta) and the green apple aphid (Aphis pomi) are widespread pest insects that reduce growth of leaves, fruits and shoots in apple (Malus × domestica). Aphid control in apple orchards is generally achieved by insecticides, but alternative management options like growing
resistant cultivars are needed for a more sustainable integrated pest management (IPM). A linkage map available for a segregating
F1-cross of the apple cultivars ‘Fiesta’ and ‘Discovery’ was used to investigate the genetic basis of resistance to aphids.
Aphid infestation and plant growth characteristics were repeatedly assessed for the same 160 apple genotypes in three different
environments and 2 consecutive years. We identified amplified fragment length polymorphism (AFLP) markers linked to quantitative
trait loci (QTLs) for resistance to D. plantaginea (‘Fiesta’ linkage group 17, locus 57.7, marker E33M35–0269; heritability: 28.3%), and to D. cf. devecta (‘Fiesta’ linkage group 7, locus 4.5, marker E32M39–0195; heritability: 50.2%). Interactions between aphid species, differences
in climatic conditions and the spatial distribution of aphid infestation were identified as possible factors impeding the
detection of QTLs. A pedigree analysis of simple sequence repeat (SSR) marker alleles closely associated with the QTL markers
revealed the presence of the alleles in other apple cultivars with reported aphid resistance (‘Wagener’, ‘Cox’s Orange Pippin’),
highlighting the genetic basis and also the potential for gene pyramiding of aphid resistance in apple. Finally, significant
QTLs for shoot length and stem diameter were identified, while there was no relationship between aphid resistance and plant
trait QTLs.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
10.
Rapid discrimination of commercial strawberry cultivars using Fourier transform infrared spectroscopy data combined by multivariate analysis 总被引:1,自引:0,他引:1
Suk Weon Kim Sung Ran Min Jonghyun Kim Sang Kyu Park Tae Il Kim Jang R. Liu 《Plant biotechnology reports》2009,3(1):87-93
To determine whether pattern recognition based on metabolite fingerprinting for whole cell extracts can be used to discriminate
cultivars metabolically, leaves and fruits of five commercial strawberry cultivars were subjected to Fourier transform infrared
(FT-IR) spectroscopy. FT-IR spectral data from leaves were analyzed by principal component analysis (PCA) and Fisher’s linear
discriminant function analysis. The dendrogram based on hierarchical clustering analysis of these spectral data separated
the five commercial cultivars into two major groups with originality. The first group consisted of Korean cultivars including
‘Maehyang’, ‘Seolhyang’, and ‘Gumhyang’, whereas in the second group, ‘Ryukbo’ clustered with ‘Janghee’, both Japanese cultivars.
The results from analysis of fruits were the same as of leaves. We therefore conclude that the hierarchical dendrogram based
on PCA of FT-IR data from leaves represents the most probable chemotaxonomical relationship between cultivars, enabling discrimination
of cultivars in a rapid and simple manner.
The authors Suk Weon Kim and Sung Ran Min contributed equally to this work. 相似文献
11.
Mustapha Ennajeh Ahmadou Mohamed Vadel Habib Khemira 《Acta Physiologiae Plantarum》2009,31(4):711-721
In this study, we compared the efficacy of defense mechanisms against severe water deficit in the leaves of two olive (Olea europaea L.) cultivars, ‘Chemlali’ and ‘Meski’, reputed drought resistant and drought sensitive, respectively. Two-year old plants
growing in sand filled 10-dm3 pots were not watered for 2 months. Changes in chlorophyll fluorescence parameters and malondialdehyde content as leaf relative
water content (RWC) decreased showed that ‘Chemlali’ was able to maintain functional and structural cell integrity longer
than ‘Meski’. Mannitol started to accumulate later in the leaves of ‘Chemlali’ but reached higher levels than in the leaves
of ‘Meski’. The latter accumulated several soluble sugars at lower dehydration. ‘Chemlali’ leaves also accumulated larger
quantities of phenolic compounds which can improve its antioxidant response. Furthermore, the activity of three antioxidant
enzymes catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) increased as leaf RWC decreased. However, differences
were observed between the two cultivars for CAT and POD but not for APX. The activity of the first two enzymes increased earlier
in ‘Meski’, but reached higher levels in ‘Chemlali’. At low leaf hydration levels, ‘Chemlali’ leaves accumulated mannitol
and phenolic compounds and had increased CAT and POD activities. These observations suggest that ‘Chemlali’ was more capable
of maintaining its leaf cell integrity under severe water stress because of more efficient osmoprotection and antioxidation
mechanisms. 相似文献
12.
Sibylle Stoeckli Karsten Mody Andrea Patocchi Markus Kellerhals Silvia Dorn 《Tree Genetics & Genomes》2009,5(1):257-267
The aim of this study was to assess the genetic basis of rust mite (Aculus schlechtendali) resistance in apple (Malus × domestica). A. schlechtendali infestation of apple trees has increased as a consequence of reduced side effects of modern fungicides on rust mites. An
analysis of quantitative trait loci (QTLs) was carried out using linkage map data available for F1 progeny plants of the cultivars ‘Fiesta’ × ‘Discovery’. Apple trees representing 160 different genotypes were surveyed for
rust mite infestation, each at three different sites in two consecutive years. The distribution of rust mites on the individual
apple genotypes was aggregated and significantly affected by apple genotype and site. We identified two QTLs for A. schlechtendali resistance on linkage group 7 of ‘Fiesta’. The AFLP marker E35M42-0146 (20.2 cM) and the RAPD marker AE10-400 (45.8 cM) were
closest positioned to the QTLs and explained between 11.0% and 16.6% of the phenotypic variability. Additionally, putative
QTLs on the ‘Discovery’ chromosomes 4, 5 and 8 were detected. The SSR marker Hi03a10 identified to be associated to one of
the QTLs (AFLP marker E35M42-0146) was traced back in the ‘Fiesta’ pedigree to the apple cultivar ‘Wagener’. This marker may
facilitate the breeding of resistant apple cultivars by marker assisted selection. Furthermore, the genetic background of
rust mite resistance in existing cultivars can be evaluated by testing them for the identified SSR marker.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
13.
Experiments were conducted to quantify parasitism of Colorado potato beetle,Leptinotarsa decemlineata (Say), by the egg parasitoid,Edovum puttleri Grissell, on 3 different cultivars of eggplant,Solanum melongena L. Levels of parasitism were higher (P<0.05) on ‘Black Pride’ than on other cultivars. The percentage of egg masses that
were parasitized was 1.2-fold higher (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’. The number of eggs per
mass that were parasitized was 1.3- and 1.4- fold greater (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’,
respectively. The percentage of eggs that were parasitized per mass and percentage of emerged adult parasitoids did not differ
(P>0.05) among cultivars; between 2.1- to 2.6- fold more females than males emerged from eggs on all cultivars during the
growing season.Edovum puttleri suppressed the 2nd generation ofL. decemlineata on ‘Black Pride’ and ‘Harris Special’, but did not suppress populations on ‘White’.
相似文献
14.
Summary Anthers and ovaries of six grapevine cultivars (three Vitis vinifera L., two V × Labruscana L. H. Bailey, and one complex hybrid) were extracted from flower buds over 2 yr and cultured on three media reported to promote
somatic embryogenesis in Vitis tissues. The highest percent embryogenesis from the hybrid ‘Chancellor’ and V. vinifera ‘Chardonnay’, ‘Merlot’, and ‘Pinot Noir’ occurred on medium C [Nitsch and Nitsch, 1969, basal medium with 3.0% (w/v) sucrose,
0.01% (w/v) inositol. 0.3% (w/v) Phytagel, 2.5 μM 2.4-dichlorophenoxyacetic acid, 2.5μM β-naphthoxyacetic acid, 5.0μM N-(2-chloro-4-pyridyl)-N′-phenylurea, and 0.05% (w/v) glutamine]. Regardless of the media, the labrusca cultivars ‘Concord’ and ‘Niagara’ produced
soft non-embryogenic callus that was sometimes mixed with well-developed somatic embryos. Nine vinifera genotypes were further
tested for several different years on medium C. Embryogenic cultures suitable for transformation were obtained from all genotypes
in more than 1 yr. The average percent embryogenesis from ovaries was 7-fold higher than from anthers. There was significant
annual variation in percent embryogenesis, demonstrating the need for media comparisons to be replicated for more than one
season. Suspension cultures suitable for use in genetic transformation were initiated from ‘Chardonnay’, ‘Merlot,’ and ‘Pinot
Noir’ pro-embryogenic masses. ‘Chardonnay’ suspension cultures plated and grown under conditions developed for recovery of
plants after biolistic transformation yielded approximately 500 non-transformed embryos per plate after 4 mo. of culture,
with 68.6% of the embryos converting to plants. This is the first reported protocol for embryogenesis from ‘Concord,’ ‘Cabernet
Franc,’ and ‘Pinot Noir’ grapevines. 相似文献
15.
Shimpei Uraguchi Masako Kiyono Takuya Sakamoto Izumi Watanabe Katsuji Kuno 《Planta》2009,230(2):267-276
The contributions of cadmium (Cd) accumulation in cell walls, antioxidative enzymes and induction of phytochelatins (PCs)
to Cd tolerance were investigated in two distinctive genotypes of black oat (Avena strigosa Schreb.). One cultivar of black oat ‘New oat’ accumulated Cd in the leaves at the highest concentration compared to another
black oat cultivar ‘Soil saver’ and other major graminaceous crops. The shoot:root Cd ratio also demonstrated that ‘New oat’
was the high Cd-accumulating cultivar, whereas ‘Soil saver’ was the low Cd-accumulating cultivar. Varied levels of Cd exposure
demonstrated the strong Cd tolerance of ‘New oat’. By contrast, low Cd-accumulating cultivar ‘Soil saver’ suffered Cd toxicity
such as growth defects and increased lipid peroxidation, even though it accumulated less Cd in shoots than ‘New oat’. Higher
activities of ascorbate peroxidase (EC 1.11.1.11) and superoxide dismutase (EC 1. 15. 1. 1) were observed in the leaves of
‘New oat’ than in ‘Soil saver’. No advantage of ‘New oat’ in PCs induction was observed in comparison to Cd-sensitive cultivar
‘Soil saver’, although Cd exposure increased the concentration of total PCs in both cultivars. Higher and increased Cd accumulation
in cell wall fraction was observed in shoots of ‘New oat’. On the other hand, in ‘Soil saver’, apoplasmic Cd accumulation
showed saturation under higher Cd exposure. Overall, the present results suggest that cell wall Cd accumulation and antioxidative
activities function in the tolerance against Cd stress possibly in combination with vacuolar Cd compartmentation. 相似文献
16.
This study analyzed genetic differences of 19 cultivars selected from somaclonal variants of Syngonium podophyllum Schott along with their parents as well as seven additional Syngonium species and six other aroids using amplified fragment length polymorphism (AFLP) markers generated by 12 primer sets. Among the 19 somaclonal cultivars, ‘Pink Allusion’ was selected from ‘White Butterfly’. Tissue culture of ‘Pink Allusion’ through organogenesis resulted in the development of 13 additional cultivars. Self-pollination of ‘Pink Allusion’ obtained a cultivar, ‘Regina Red Allusion’, and tissue culture propagation of ‘Regina Red Allusion’ led to the release of five other cultivars. The 12 primer sets generated a total of 1,583 scorable fragments from all accessions, of which 1,284 were polymorphic (81.9%). The percentages of polymorphic fragments within ‘White Butterfly’ and ‘Regina Red Allusion’ groups, however, were only 1.2% and 0.4%, respectively. Jaccard's similarity coefficients among somaclonal cultivars derived from ‘White Butterfly’ and ‘Regina Red Allusion’, on average, were 0.98 and 0.99, respectively. Seven out of the 15 cultivars from the ‘White Butterfly’ group and three out of six from the ‘Regina Red Allusion’ group were clearly distinguished by AFLP analysis as unique fragments were associated with respective cultivars. The unsuccessful attempt to distinguish the remaining eight cultivars from the ‘White Butterfly’ group and three from the ‘Regina Red Allusion’ group was not attributed to experimental errors or the number of primer sets used; rather it is hypothesized to be caused by DNA methylation and/or some rare mutations. This study also calls for increased genetic diversity of cultivated Syngonium as they are largely derived from somaclonal variants. 相似文献
17.
E. A. Popowich A. P. Firsov T. Y. Mitiouchkina V. L. Filipenya S. V. Dolgov V. N. Reshetnikov 《Plant Cell, Tissue and Organ Culture》2007,90(3):237-244
Transgenic plants of hyacinth (Hyacinthus orientalis L.) cvs. Edisson and Chine Pink have been obtained by Agrobacterium-mediated transformation. Leaf explants of the both hyacinth cultivars regenerated shoots on MS medium containing 2.2 μM BAP
and 0.3 μM NAA at a frequency of 95%. A. tumefaciens strain CBE21 carrying binary vector pBIThau35 was used for transformation. Plasmid pBIThau35 has been produced by cloning
preprothaumatin II cDNA into pBI121 instead of uidA gene. Inoculated leaf explants formed calli and shoots at high frequency on selective medium with 100 mg l−1 kanamycin. Four hyacinth transgenic lines of cv. Chine Pink and one line of cv. Edisson have been selected on medium containing 200 mg l−1 kanamycin. The insertion of thaumatin II gene into hyacinth genome has been confirmed by PCR-analysis. All transgenic plants
expressed substantial amounts of thaumatin II (between 0.06 and 0.28% of the total soluble protein). Hyacinth transgenic lines
were assayed for resistance to the pathogenic fungi Fusarium culmorum and Botrytis cinerea. There were no significant differences between nontransformed control and transgenic leaves of both cultivars. At the same
time the bulbs of the transgenic line Н7401 cv. Chine Pink showed the higher level of resistance to B. cinerea, the bulbs of the transgenic line Н7404 were more resistant to F. culmorum. In both cases the signs of the fungal disease were developed more slowly. The resistance of the bulbs cv. Edisson line to these fungi was not changed. All transgenic hyacinth plant were successfully transferred to soil for further evaluation. 相似文献
18.
V. G. M. Bus D. Chagné H. C. M. Bassett D. Bowatte F. Calenge J.-M. Celton C.-E. Durel M. T. Malone A. Patocchi A. C. Ranatunga E. H. A. Rikkerink D. S. Tustin J. Zhou S. E. Gardiner 《Tree Genetics & Genomes》2008,4(2):223-236
Woolly apple aphid (WAA; Eriosoma lanigerum Hausm.) can be a major economic problem to apple growers in most parts of the world, and resistance breeding provides a sustainable
means to control this pest. We report molecular markers for three genes conferring WAA resistance and placing them on two
linkage groups (LG) on the genetic map of apple. The Er1 and Er2 genes derived from ‘Northern Spy’ and ‘Robusta 5,’ respectively, are the two major genes that breeders have used to date
to improve the resistance of apple rootstocks to this pest. The gene Er3, from ‘Aotea 1’ (an accession classified as Malus sieboldii), is a new major gene for WAA resistance. Genetic markers linked to the Er1 and Er3 genes were identified by screening random amplification of polymorphic deoxyribonucleic acid (DNA; RAPD) markers across DNA
bulks from resistant and susceptible plants from populations segregating for these genes. The closest RAPD markers were converted
into sequence-characterized amplified region markers and the genome location of these two genes was assigned to LG 08 by aligning
the maps around the genes with a reference map of ‘Discovery’ using microsatellite markers. The Er2 gene was located on LG 17 of ‘Robusta 5’ using a genetic map developed in a M.9 × ‘Robusta 5’ progeny. Markers for each of
the genes were validated for their usefulness for marker-assisted selection in separate populations. The potential use of
the genetic markers for these genes in the breeding of apple cultivars with durable resistance to WAA is discussed. 相似文献
19.
S. Jayasankar Richard E. Litz Dennis J. Gray Pamela A. Moon 《In vitro cellular & developmental biology. Plant》1999,35(6):475-479
Summary
Colletotrichum gloeosporioides Penz., the causal agent of mango anthracnose, produces a phytotoxin in vitro. The partially purified phytotoxin, presumably
colletotrichin, caused anthracnose-like symptoms on young mango leaves, was toxic to embryogenic suspension cultures of two
mango cultivars, ‘Hindi’ and ‘Carabao,’ and inhibited in vitro seed germination of two nonhosts, lettuce and tobacco. There
were linear relationships between concentration of the partially purified phytotoxin and mortality of mango embryogenic cultures.
Embryogenic cultures grown in the presence of the partially purified phytotoxin showed significantly lower growth rates than
the controls. Similarly, embryogenic cultures grown in the presence of 40% (vol/vol) fungal culture filtrate showed significantly
lower growth rates than unchallenged controls. Medium containing 40% (vol/vol) Czapek-Dox fungal broth did not reduce growth
of embryogenic cultures, indicating the production of phytotoxin in vitro. The results suggest that either fungal culture
filtrate or purified phytotoxin can be used as in vitro selection agents to screen for resistance to this fungus. 相似文献
20.
M. J. van Steenis K. A. M. H. El-Khawass 《Entomologia Experimentalis et Applicata》1995,76(2):121-131
Life table data forAphis gossypii Glover (Homoptera: Aphididae), an important pest in glasshouse cucumber crops, were studied at 20, 25 and 30°C on two cucumber
cultivars (Cucumis sativus L.) in controlled climate cabinets. The development time on the cucumber cv. ‘Sporu’ ranged from 4.8 days at 20°C to 3.2
days at 30°C. Immature mortality was approximately 20% and did not differ between temperatures. Most mortality occurred during
the first instar. Reproduction periods did not differ among temperatures, but at 25 and 30°C more nymphs were produced (65.9
and 69.8 nymphs/♀, respectively) than at 20°C (59,9 nymphs/♀) because of a higher daily reproduction. Intrinsic rate of increase
was greatest at 25°C (r
m
=0.556 day−1). At 20 and 30°C the intrinsic rate of increase was 0.426 and 0.510, respectively. On cv. ‘Aramon’, the development time
ofA. gossypii was approximately 20% longer at all temperatures. Immature mortality did not differ between the two cultivars. The intrinsic
rate of increase on cv. ‘Aramon’ was 15% smaller than on cv. ‘Sporu’. The use of cucumber cultivars partially resistant to
aphids is discussed in relation to biological control of cotton aphid in glasshouses. Development time and immature mortality
on leaves of the middle and upper leaf layer of glasshouse grown cucumber plants (cv. ‘Aramon’) were comparable to development
in the controlled climate cabinets. On the lower leaves immature mortality was much higher (approximately 82%) than on leaves
of the middle (24.0%) and upper leaf layer (24.5%). Reproduction was less on the lower leaf layer (45.9, 70.5 and 70.1 nymphs/♀
on leaves of the lower, middle and upper leaf layer, respectively).
Aphids, successfully parasitized byAphidius colemani Viereck (Hymenoptera: Braconidae) only reproduced when they were parasitized after the third instar. Fecundity was 0.1 to
0.9 and 10.5 to 13.3 nymphs/♀ for aphids parasitized in the fourth instar or as adults, respectively. Reproduction of aphids
that were stung but survived the attack was lower than for aphids not stung. Average longevity of these aphids was equal to
the longevity of aphids not stung byA. colemani. 相似文献