共查询到19条相似文献,搜索用时 125 毫秒
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张仲伦 《生物化学与生物物理进展》2000,27(3):333-334
微弱发光分析技术已经用于肿瘤学研究,骨肿瘤病人和正常人的血液和尿液的发光强度使用BPCL型微弱发光测量仪进行了测量.结果指出,骨肿瘤病人血液和尿液的发光强度高于正常人(P<0.05).骨肿瘤病人尿液的发光强度在手术之后明显降低(P<0.05).裸鼠血液和各种脏器的微弱发光测量结果表明,荷瘤之后,各个脏器的发光强度显著增加. 相似文献
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张仲伦 《生物化学与生物物理进展》2000,27(3):333-334
微弱发光分析技术已经用于肿瘤学研究,骨肿瘤病人和正常人的血液和尿液的发光强度使用BPCL型微弱发光测量仪进行了测量,结果指出,骨肿瘤病人血液和尿液的发光强度高于正常人(P〈0.05),骨肿瘤病人尿液的发光强度在手术之后明显降低(P〈0.05)。裸鼠血液和各种脏器的微弱发光测量结果表明,荷瘤之后,各个脏器的发光强度显著增加。 相似文献
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绿豆芽超微弱发光的二维图像探测 总被引:1,自引:0,他引:1
采用微通道板像增强研制了一种能探测极弱光图像的超高灵敏度光电探测系统,能够探测到0.5Photons/mm2·s(阴极灵敏度)的极弱发光图像,是目前弱光图像探测器中灵敏度最高的。应用上述的光电探测系统,进行了绿豆芽超微弱发光二维图像探测的研究,首次得到了以下结论:1.绿豆发芽时存在超微弱发光现象,发光强度在104-105Photons/s·cm2的范围内;2.子叶和幼叶的发光高于幼茎的发光;3.在避先保持10分钟豆芽发光衰减至稳定后,绿豆芽仍能维持一定的发光。上述结论为超微弱发光现象的生物学理论研究和医学及环境保护中的应用提供了实验依据。 相似文献
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微弱发光分析技术原理及应用实例(一) 总被引:10,自引:0,他引:10
张仲伦 《生物化学与生物物理进展》1999,26(4):405-407
微弱发光分析技术近年得到迅速发展,在自由基、活性氧分析、化学发光分析、生物的超微弱发光分析、发光免疫分析、生物发光分析等领域得到广泛应用.简要介绍了微弱发光分析技术的测量原理,并以一些研究成果为实例讲解如何应用微弱发光分析技术进行研究和实践. 相似文献
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生物系统超弱发光的探测:绿豆、大鼠血液和3T3细胞的发光 总被引:9,自引:0,他引:9
本文报道一台用于生物系统微弱发光研究的高灵敏单光子计数系统、能在200—900nm范围测量生物样品的发光强度、光谱和发光动力学.由于光电倍增管以液氮冷却、噪声降至40cps,在99.9%置信度和6小时测量条件下,最小可探测0.3光子/秒、厘米~2的微弱光子流.用该系统研究了萌发绿豆、大鼠血液和体外培养正常和转化3T3细胞的发光.这些样品的发光都含有一个光诱导成份,且以不同的速率衰减、最后达到稳定水平代表了这些生物系统自发的代谢发光.实验发现,转化的3T3细胞的发光强度比正常细胞约低30%. 相似文献
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水稻种子萌发期超微弱光子计数成像研究 总被引:3,自引:1,他引:2
运用PIAS捕获了水稻种子萌发期自发辐射的超微弱光子。光子长时间累积形成了二维图像。研究表明:萌发种子整体均发光,胚根和胚芽及其生长点发较强的光;水稻种子萌发期光子辐射强度呈现双峰值规律,第二峰前期光子数和根生长长度与萌发时间均有自然对数关系,根生长激增迟后于光子数激增 相似文献
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H. Reiber 《Luminescence》1989,4(1):245-248
Cellular low-level luminescence was measured after various disintegrative processes in brain cell preparations. In addition to known origins of low-level luminescence, e.g. oxygen radical reactions or enzymatic and non-enzymatic redox systems, a further source of photon emission is reported which is independent of external oxygen, oxygen radicals and enzyme activities. Vital cells from rat brain homogenates or pig oligodendrocytes could be kept for hours at 37 °C without any photon emission. Only after disintegrative processes a cellular photon emission could be induced. The maximal intensity of about 400 impulses/s/mg protein and a total radiation of about 6 × 106 I/mg depended on the type of cells. The signal could be retained completely at 4 °C or in frozen samples. Heating (10 min, 90 °C) did not suppress the photon emission. Luminol and lucigenin did not amplify the signal as is usually observed in oxygen radical-producing cells. Non-specific radical scavengers as well as detergents suppressed the cellular photon emission completely. It is suggested that this cellular luminescence represents a biophysical radiation which originates from the interruption of an intermolecular radiationless energy transfer. 相似文献
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N L Vekshin 《Journal of biochemical and biophysical methods》1987,15(2):97-104
The movement of pyrene in a lipid bilayer is shown to occur not only in the lateral but also transmembrane direction. Within the excited state lifetime, the pyrene monomer elevates from the depth to the polar region of the membrane and emits a luminescence photon. The excimer does not exhibit any marked transmembrane movement. The luminescence quenching efficiency of monomers and excimers depends on the depth of penetration of the quencher into the membrane. In the lipid bilayer, pyrene luminescence is strongly quenched by oxygen. The binding of pyrene to membrane proteins protects it from quenching. It has been concluded that the widely used estimations of membrane viscosity from pyrene luminescence intensity are incorrect. 相似文献
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为了寻找最佳测试条件来实现猪肉样品新鲜度的检测,采用单光子测试系统测试了不同条件下猪肉的自发辐射强度和延迟发光的变化。4天连续测试的噪声平均值为76.3 counts/s,平均偏差为1.1 counts/s;自发辐射测试中肥肉样品信号较强,其强度随放置时间的增加有增加过程;延迟发光测试中大排及肥肉信号较强;按照延迟发光动力学曲线拟合,拟合系数达0.996。随着测量时间的增加,延迟发光的特征时间t降低,反应了猪肉新鲜度下降。实验结果表明该系统能实现对生鲜猪肉新鲜度的直接检测,具有选材少,检测速度快的特点。 相似文献
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E. Newton Harvey 《The Journal of general physiology》1925,8(2):89-108
Methods are described for measuring the light emitted by an emulsion of luminous bacteria of given thickness, and calculating the light emitted by a single bacterium, measuring 1.1 x 2.2 micra, provided there is no absorption of light in the emulsion. At the same time, the oxygen consumed by a single bacterium was measured by recording the time for the bacteria to use up .9 of the oxygen dissolved in sea water from air (20 per cent oxygen). The luminescence intensity does not diminish until the oxygen concentration falls below 2 per cent, when the luminescence diminishes rapidly. Above 2 per cent oxygen (when the oxygen dissolving in sea water from pure oxygen at 760 mm. Hg pressure = 100 per cent) the bacteria use equal amounts of oxygen in equal times, while below 2 per cent oxygen it seems very likely that rate of oxygen absorption is proportional to oxygen concentration. By measuring the time for a tube of luminous bacteria of known concentration saturated with air (20 per cent oxygen) to begin to darken (2 per cent oxygen) we can calculate the oxygen absorbed by one bacterium per second. The bacteria per cc. are counted on a blood counting slide or by a centrifugal method, after measuring the volume of a single bacterium (1.695 x 10–12 cc.). Both methods gave results in good agreement with each other. The maximum value for the light from a single bacterium was 24 x 10–14 lumens or 1.9 x 10–14 candles. The maximum value for lumen-seconds per mg. of oxygen absorbed was 14. The average value for lumen-seconds per mg. O2 was 9.25. The maximum values were selected in calculating the efficiency of light production, since some of the bacteria counted may not be producing light, although they may still be using oxygen. The "diet" of the bacteria was 60 per cent glycerol and 40 per cent peptone. To oxidize this mixture each mg. of oxygen would yield 3.38 gm. calories or 14.1 watts per second. 1 lumen per watt is therefore produced by a normal bacterium which emits 14 lumen-seconds per mg. O2 absorbed. Since the maximum lumens per watt are 640, representing 100 per cent efficiency, the total luminous efficiency if .00156. As some of the oxygen is used in respiratory oxidation which may have nothing to do with luminescence, the luminescence efficiency must be higher than 1 lumen per watt. Experiments with KCN show that this substance may reduce the oxygen consumption to 1/20 of its former value while reducing the luminescence intensity only ¼. A partial separation of respiratory from luminescence oxidations is therefore effected by KCN, and our efficiency becomes 5 lumens per watt, or .0078. This is an over-all efficiency, based on the energy value of the "fuel" of the bacteria, regarded as a power plant for producing light. It compares very favorably with the 1.6 lumens per watt of a tungsten vacuum lamp or the 3.9 lumens per watt of a tungsten nitrogen lamp, if we correct the usual values for these illuminants, based on watts at the lamp terminals, for a 20 per cent efficiency of the power plant converting the energy of coal fuel into electric current. The specific luminous emission of the bacteria is 3.14 x 10–6 lumens per cm2. One bacterium absorbs 215,000 molecules of oxygen per second and emits 1,280 quanta of light at λmax = 510µµ. If we suppose that a molecule of oxygen uniting with luminous material gives rise to the emission of 1 quantum of light energy, only 1/168 of the oxygen absorbed is used in luminescence. On this basis the efficiency becomes 168 lumens per watt or 26.2 per cent. 相似文献