中国沟胫天牛亚科一新属及一新种（鞘翅目：天牛科）

本文记述天牛科沟胫天牛亚科１新属，球胸天牛属Ｇｉｂｂｏｈａｍｍｕｓｇｅｎ．ｎｏｖ．和１新种：缩颈球胸天牛Ｇｉｂｂｏｈａｍｍｕｓｓｔｒｉｃｔｉｃｏｌｌｉｓｓｐ．ｎｏｖ。     

中国花天牛亚科三新种记述：鞘翅目：天牛科

本文了中国花天牛亚科３新种－卵斑长颊瘦花牛Ｇｎａｔｈｏｓｔｒａｎｇａｌｉａ ｅｌｌｉｐｔｉｃａ,ｓｐ．ｎｏｖ。,暗背脊花天牛Ｓｔｅｎｏｃｏｒｕｓ ｆｕｓｃｏｄｏｒａｌｉｓ,ｓｐ．ｎｏｖ．,四斑短腿瘦花天牛Ｐｅｄｏｓｔｒａｎｇａｌｉａ ｑｕａｄｒｉｍａｃｕｌａｔａ,ｓｐ．ｎｏｖ。模式标本存西农业大学昆虫标本馆。     

中国泥色天牛属Uraecha Thomson分类研究（鞘翅目：天牛科：沟胫天 …

记述泥色天牛属Ｕｒａｅｃｈａ的主要特征及地理颁由,并报道１新种：龙州泥色天牛Ｕｒａｅｃｈａ ｌｏｎｇｚｈｏｕｅｎｓｉｓ,ｓｐ．ｎｏｖ．,编制了我国已知９种的检索表。新种的模式标本保存在西南农业大学听话纱昆虫标本馆。     

中国污天牛属二新种记述(鞘翅目:天牛科)

记述了中国污天牛属Ｍｏｒｃｈｏｔｙｐａ ２新种，即斑胸污天牛Ｍ．ｎｉｇｒｉｃｏｌｌｉｓ Ｗａｎｇ ｅｔ Ｊｉａｎｇ，ｓｐ．ｎｏｖ．和瘤胸污天牛Ｍ．ｔｕｂｅｒｃｕｌｉｃｏｌｌｉｓ Ｗａｎｇ ｅｔ Ｊｉａｎｇ，ｓｐ．ｎｏｖ。模式标本保存在西南农业大学昆虫标本馆。     

中国楔天牛族二新种(鞘翅目:天牛科:沟胫天牛亚科)

记述了沟胫天牛亚科楔天牛族2新种,即黑斑双脊天牛Paraglenea nigromaculata sp.nov。和黑缘并脊天牛Glenea nigromarginella sp.nov。,新种模式标本保存在西南农业大学植保系昆虫标本馆。     

中国花天牛亚科三新种记述（鞘翅目：天牛科）

训国花天牛亚科３新种－－黑角瘤花天牛Ｇａｕｒｏｔｉｎａ ｎｉｇｒｏａｎｔｅｎａｔａ,ｓｐ．ｎｏｖ．,青海皮花一在牛Ｒｈａｇｉｕｍ ｑｉｎｇｈａｉｅｎｓｉｓ,ｓｐ．ｎｏｖ．,四面山华花天牛Ｓｉｎｏｓｔｒａｎｇａｌｉｓｓｉｍｉａｎｓｈａｎａ,ｓｐ．ｎｏｖ．。模式标本存西南农业大学昆虫标本馆。     

云南天牛科三新种记述：鞘翅目：天牛科

记述了采自云南的沟胫天牛亚科３新种：齿尾毡天牛Ｔｈｙｌａｃｕｓｄｅｎｔｉｐｅｎｎｉｓ、黑斑灰天牛Ｂｌｅｐｅｐｈａｅｕｓｎｉｇｒｏｓｔｉｇｍａ和云南刺锦天牛Ｓｔｅｒｎｏｈａｍｍｕｓｙｕｎｎａｎａ。其中,刺锦天牛属ＳｔｅｒｎｏｈａｍｍｕｓＢｒｅｕｎｉｎｇ为我国首次记载。新种模式标本保存于西南农业大学昆虫标本馆。     

广西地区蚱科三新种记述(直翅目:蚱总科)

本文记述采自广西壮族自治区蚱科３新种,即瘤米蚱Ｍｉｓｈｔｓｈｅｎｋｏｔｅｔｉｘｔｕｂｅｒｃｕｌａｔａｓｐ．ｎｏｖ．、拟喀蚱Ｔｅｔｒｉｘｃｅｐｅｒｏｉｄｅｓｓｐ．ｎｏｖ．及粗股蚱Ｔｅｔｒｉｘｇｒｏｓｉｆｅｍｕｒａｓｐ．ｎｏｖ．。     

四川省阳蝇属三新种：双翅目：蝇科

阳蝇属三新种,双鬃阳蝇,新种Ｈｅｌｉｎａｄｕｐｌｉｃｉｓｅｔａｓｐ．ｎｏｖ;大斑阳蝇,新种Ｈｅｌｉｎａｍａｇｎｉｍｕｃｕｌａｔａｓｐ．ｎｏｖ。欢愉阳蝇,新种Ｈｅｌｉｎｉａｈｅｓｔａｓｐ．ｎｏｖ。模式标本Ｉ号存华西医科大学,Ⅱ,Ⅲ号存在于雅安地区卫生防疫站。     

中国管巢蛛属六新种(蜘蛛目:管巢蛛科)

本文记述管巢蛛属Ｃｌｕｂｉｏｎａ６新种,即环管巢蛛Ｃｌｕｂｉｏｎａｃｉｒｃｕｌａｔａｓｐ．ｎｏｖ．,双齿管巢蛛Ｃｌｕｂｉｏｎａｄｉｄｅｎｔａｔａｓｐ．ｎｏｖ．,丝歧管巢蛛Ｃｌｕｂｉｏｎａｆｉｌｏｒａｍｕｌａｓｐ．ｎｏｖ．,异管管巢蛛Ｃｌｕｂｉｏｎａｈｅｔｅｒｏｄｕｃｔａｓｐ．ｎｏｖ．,曲管巢蛛Ｃｌｕｂｉｏｎａｔｏｒｔｕｏｓａｓｐ．ｎｏｖ．,横列管巢蛛Ｃｌｕｂｉｏｎａｔｒａｎｓｖｅｒｓａｓｐ．ｎｏｖ．。     

Quadruplexes of human telomere DNA analogs designed to contain G:A:G:A,G:G:A:A,and A:A:A:A tetrads

Replacement of two to four guanines by adenines in the human telomere DNA repeat dG₃(TTAG₃)₃ did not hinder the formation of quadruplexes if the substitutions took place in the terminal tetrad bridged by the diagonal loop of the intramolecular antiparallel three‐tetrad scaffold, as proved by CD and PAGE in both Na⁺ and K⁺ solutions. Thermodynamic data showed that, in Na⁺ solution, the dG₃(TTAG₃)₃ quadruplex was destabilized, the least by the two G:A:G:A tetrads, the most by the G:G:A:A tetrad in which the adenosines replaced syn‐guanosines. In physiological K⁺ solution, the highest destabilization was caused by the 4A tetrad. In K⁺, only the unmodified dG₃(TTAG₃)₃ quadruplex rearranged into a K⁺‐dependent quadruplex form, none of the multiple adenine‐modified structures did so. This may imply biological consequences for nonrepaired A‐for‐G mutations. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 880–886, 2010.     

Oka: Behind the Barricades: Kanehsatake: 270 Years of Resistance: Spudwrench: Kahnawake Man: My Name is Kahentiiosta: Acts of Defiance

Oka: Behind the Barricades. 1998. 312 minutes. For more information contact the National Film Board of Canada, 3155 Côte de Lisse Rd., St. Laurent, Quebec H4N 2N4. Series of four videos:
Kanehsatake: 270 Years of Resistance. 1993. 120 minutes. Directed by Alanis Obomsawin .
Spudwrench: Kahnawake Man. 1997. 58 minutes. Directed by Alanis Obomsawin .
My Name is Kahentiiosta. 1995. 30 minutes. Directed by Alanis Obomsawin .
Acts of Defiance. 1992. 104 minutes. Directed by Alec MacLeod .     

Experimental demonstration of T:(G:G:G:G):T hexad and T:A:A:T tetrad alignments within a DNA quadruplex stem

A template-based approach was used to design unprecedented architectural motifs into a known DNA framework. The structure formed by the sequence d(GCGGTTGGAT) in 0.1 M Na(+) solution has been determined using molecular dynamics simulations constrained by distance and dihedral restraints derived from NMR experiments. The molecular topology has been previously observed for the sequence d(GCGGTGGAT) (Webba da Silva, M. (2003) Biochemistry 42, 14356-65). Insertion of a single thymine into the double chain reversal formed by the segment GGTGG results in the unprecedented experimental demonstration of a T:(G:G:G:G):T hexad. The bi-stranded hexad results from the pairing alignment of two G(T-G) triads. Each triad results from recognition of the sheared edge of a guanine by the Watson-Crick edge of a thymine of the segment GGTTGG. The alignment is stabilized by base-stacking of the thymine to the sugar pucker of the preceding thymine. The latter is involved in formation of the T:A:A:T tetrad alignment by forming a hydrogen bond with the free amino proton of a Watson-Crick aligned A:A mispair. We have thus established that residues in double chain reversal loops linking juxtaposed tetrads of a quadruplex stem may facilitate formation of yet unknown hydrogen bond alignments. By employing a systematic approach analysis of sequence motifs appearing in double chain reversals, bridging tetrad layers should allow for the prediction of topologies and architectural motifs appearing in biologically relevant genomic regions.     

A dimeric RNA quadruplex architecture comprised of two G:G(:A):G:G(:A) hexads,G:G:G:G tetrads and UUUU loops

Using CD and NMR, we determined the structure of an RNA oligomer, r(GGAGGUUUUGGAGG) (R14), comprising two GGAGG segments joined by a UUUU segment. A modified quadruplex structure was observed for r(GGAGGUUUUGGAGG) in solution even in the absence of K(+). An unusually stable dimeric RNA quadruplex architecture formed from two strands of r(GGAGGUUUUGGAGG) at low K(+) concentration is reported here. In each strand of r(GGAGGUUUUGGAGG), two sets of successive turns in the GGAGG segments and turns at both ends of the UUUU loops drive four G-G steps to align in a parallel manner, a core with two stacked G-tetrads being formed. Two adenine bases bind to two edges of one G:G:G:G tetrad through the sheared G:A mismatch augmenting the tetrad into a G:G(:A):G:G(:A) hexad. Thus, one molecule of r(GGAGGUUUUGGAGG) folds into a modified quadruplex comprising a G:G:G:G tetrad, a UUUU double-chain reversal loop and a G:G(:A):G:G(:A) hexad. Two such molecules further associate by stacking through the dimeric hexad-hexad interface with a rotational symmetry. The ribose rings of most nucleotides take S (close to C2'-endo) puckering, which is unusual for an RNA. K(+) can increase the stability of this quadruplex structure; the number of bound K(+) was estimated from the results of the titration experiment. Besides G:G and G:A mismatches, a network of hydrogen bonds including O4'-NH(2) and C-H..O hydrogen bonds, and the extensive base stacking contribute to the high thermodynamic stability of R14. Our results could provide the stereochemical and thermodynamic basis for elucidating the biological role of the GGAGG-containing RNA segments abundantly existing in various RNAs. Relevance to quadruplex-mediated mRNA-FMRP binding and HIV-1 genome RNA dimerization is discussed.     

Focus: Microbiome: Metagenomic Assembly: Overview,Challenges and Applications

Advances in sequencing technologies have led to the increased use of high throughput sequencing in characterizing the microbial communities associated with our bodies and our environment. Critical to the analysis of the resulting data are sequence assembly algorithms able to reconstruct genes and organisms from complex mixtures. Metagenomic assembly involves new computational challenges due to the specific characteristics of the metagenomic data. In this survey, we focus on major algorithmic approaches for genome and metagenome assembly, and discuss the new challenges and opportunities afforded by this new field. We also review several applications of metagenome assembly in addressing interesting biological problems.