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1.
为明确食物中重金属离子在蜘蛛体内的传递、生物放大及对其生长历期和耐饥力的影响,采用原子吸收光谱法检测了拟水狼蛛Pirata subpiraticus取食加Cd2+食物后体内Cd2+含量变化情况,测定了Cd2+对其发育历期和耐饥力的间接影响。结果表明:食物中的Cd2+能够通过食物链进行传递并在拟水狼蛛体内积累,积累量随拟水狼蛛龄期的增长而增加,积累量与消耗的黑腹果蝇Drosophila melanogaster数量极显著正相关(P<0.01), 与消耗食物中Cd2+含量显著正相关(P<0.05); 对食物中Cd2+的吸收率为65.4%,生物放大因子为1.8。成蛛改喂无Cd2+的黑腹果蝇后在观察的4周内体内Cd2+含量变化不明显。食物中的Cd2+能导致拟水狼蛛的发育历期显著延长、耐饥力显著降低。研究结果可为进一步研究环境中Cd2+沿土壤-昆虫-天敌传递、放大和生理耐受等提供理论依据。  相似文献   

2.
Cd2+胁迫对银芽柳PSⅡ叶绿素荧光光响应曲线的影响   总被引:2,自引:0,他引:2  
钱永强  周晓星  韩蕾  孙振元  巨关升 《生态学报》2011,31(20):6134-6142
以盆栽银芽柳为材料,利用MINI-IMAGING-PAM荧光成像测定系统,研究了Cd2+胁迫下叶片叶绿素荧光参数的变化及其光响应曲线。结果表明,初始荧光Fo与最大荧光Fm随着Cd2+浓度的增大而呈现先升后降的趋势,Fo与Fm在200 mg/LCd2+处理4周时达到最高值,400 mg/LCd2+处理则显著下降;PSⅡ最大光化学效率(Fv/Fm)与PSⅡ潜在光化学效率(Fv/Fo)显著受 Cd2+胁迫抑制,但随Cd2+浓度的增加呈先降后升的变化趋势。Cd2+胁迫下各叶绿素荧光参数的光响应结果表明,PSⅡ实际光量子效率Y(Ⅱ)、荧光淬灭系数(qP)随光化光强度的增加呈下降趋势,而同光强下高浓度Cd2+ 使Y(Ⅱ)与(qP) 显著降低;PSⅡ调节性能量耗散的量子产额Y(NPQ)、非光化学淬灭系数(qN)与表观电子传递速率(ETR)则随着光强增加呈上升趋势,同光强下高浓度Cd2+处理显著提高Y(NPQ)qN 与ETR。Cd2+胁迫下,PSⅡ非调节性能量耗散的量子产额Y(NO)稳定在较低水平,同光强下Y(NO)随Cd2+浓度增加略有提高。说明,银芽柳通过调节PSⅡ反应中心开放程度与活性,对Cd2+胁迫表现出较强的耐性,高浓度Cd2+胁迫导致PSⅡ反应中心关闭或不可逆失活,表现出光抑制。  相似文献   

3.
许丹丹  席贻龙  马杰  葛雅丽 《生态学报》2011,31(17):4874-4880
采用急性毒性试验研究了(25±1)℃下Cd2+对角突臂尾轮虫(Brachionus angularis)和曲腿龟甲轮虫(Keratella valga)的24 h LC50值, 采用生命表实验方法研究了(25±1)℃下、以密度为1.0×106 个细胞/mL的斜生栅藻为轮虫食物时不同浓度(7.0、12.0、20.4、34.6、58.8,100.0 μg/L)的Cd2+对角突臂尾轮虫和曲腿龟甲轮虫生命表统计学参数的影响。结果表明, Cd2+对角突臂尾轮虫和曲腿龟甲轮虫幼体的24 h LC50值分别为95.5 μg/L和231.9 μg/L。Cd2+浓度对角突臂尾轮虫的种群内禀增长率具有显著的影响(P<0.05), 对曲腿龟甲轮虫的世代时间、生命期望和净生殖率具有显著的影响(P<0.05)。与对照组相比, 100.0 μg/L的Cd2+显著降低了角突臂尾轮虫的种群内禀增长率; 34.6 μg/L和58.8 μg/L的Cd2+显著降低了曲腿龟甲轮虫的世代时间, 34.6和100.0 μg/L的Cd2+显著降低了曲腿龟甲轮虫的生命期望, 34.6 μg/L的Cd2+显著降低了曲腿龟甲轮虫的净生殖率。角突臂尾轮虫对Cd2+污染的敏感性较曲腿龟甲轮虫强, 各生命表统计学参数对污染物的敏感性因轮虫种类的不同而异。  相似文献   

4.
结缕草对重金属镉的生理响应   总被引:4,自引:0,他引:4  
采用水培的方法研究了不同浓度的Cd2+对结缕草叶片枯黄程度的影响,结果表明5mg/L、10mg/L和30mg/L Cd2+处理对结缕草叶片的枯黄比例无显著影响;以10mg/L 的Cd2+处理结缕草植株,研究了结缕草对镉的生理响应。与对照相比,Cd2+处理30d时结缕草叶片的超氧阴离子自由基(O-2·)含量、丙二醛(MDA)含量和相对电导率(RC)显著升高,升高幅度分别为58.69%、38.52%和68.69%,表明Cd2+对结缕草叶片产生了氧化胁迫。与对照相比,Cd2+处理30d时最大量子产量(Fv/Fm)和实际量子产量(Fv'/Fm')分别显著下降3.90%和11.36%;处理20d时实际光化学效率(ΦPSⅡ)和相对电子传递速率(rETR)分别显著下降22.22%、22.66%,处理30d时分别显著上升12.5%、18.12%;处理20d时光化学猝灭系数(qp)显著下降14.29%,非光化学猝灭系数(NPQ)则显著上升38.54%,表明结缕草叶片的光反应机构受到了伤害,而光保护能力得到了提高。与对照相比,Cd2+处理下超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性先升高后降低,而过氧化物酶(POD)活性先降低后升高;脯氨酸、可溶性糖含量升高,处理30d时可溶性糖含量显著上升63.35%;处理前20d时可溶性蛋白的含量高于对照,处理30d时却低于对照。随着Cd2+处理时间的增加,结缕草地下部Cd含量逐渐升高;地上部Cd含量先升高后降低,差异显著;处理20d时地上部镉含量最高,转运系数最高,达到45.84%;处理10d时镉在结缕草地上部的分布比例小于地下部,而处理20d、30d时地上部镉的分布比例占优势,表明结缕草具备修复植物的潜质。镉在结缕草地下部水溶态所占比例最大,其次是氯化钠和醋酸提取态;与地下部不同,结缕草地上部醋酸提取态镉的含量要高于氯化钠提取态。综上,结缕草对Cd2+具有一定的耐受性。  相似文献   

5.
对两种多孔菌科大型真菌槐栓菌(Trametes robiniophila)和木蹄层孔菌(Fomes fomentarius)子实体生物吸附Cd2+的影响因素(包括吸附剂用量、初始pH、吸附时间、初始Cd2+浓度)和吸附特性进行分析。结果表明,槐栓菌和木蹄层孔菌对低浓度的Cd2+(10 mg/L)吸附的最适pH为6;Cd2+的去除率随吸附剂用量和吸附时间的增加而增大,槐栓菌和木蹄层孔菌均在吸附剂用量为2g/L时达到吸附平衡,槐栓菌在吸附时间为30 min时达到吸附平衡,而木蹄层孔菌在吸附时间为60 min时达到吸附平衡;槐栓菌和木蹄层孔菌对10 mg/L Cd2+的最大去除率分别为98%和94%。Langmuir等温吸附平衡模型比Freundlich等温吸附平衡模型能更好的拟合两种大型真菌对Cd2+的吸附过程;槐栓菌和木蹄层孔菌对10 mg/L Cd2+的最大吸附量分别为17.40 mg/g和8.91 mg/g。对实验数据进行动力学模型拟合可知,两种大型真菌对Cd2+的生物吸附过程均符合准二阶动力学模型。槐栓菌和木蹄层孔菌生物吸附低浓度Cd2+的化学反应机理可能为离子交换。  相似文献   

6.
张征田  夏敏  彭宇  杜瑞卿 《昆虫学报》2009,52(9):994-999
为了探明重金属含量对拟水狼蛛Pirata subpiraticus生物学特性的影响, 本研究于2008年7月在河南南阳地区5种不同生境下, 共采集50份土壤样本和150头拟水狼蛛样本, 采用原子吸收光谱法测定了5种不同生境下雌拟水狼蛛体内重金属含量, 并测定了雌蛛头胸甲宽、体重、卵袋重量、卵数目和卵体积等生物学指标。结果表明:不同样点拟水狼蛛重金属含量与土壤重金属含量显著正相关, 4个重金属污染区与宝天曼自然保护区(Baotianman, BTM)拟水狼蛛体内Cd、Cu和Zn的含量差异极显著(P<0.001), 桐柏铜矿(Tongbai Tongkuang, TBTK)和南阳油田(Nanyang Youtian, NYYT) 的Pb含量与BTM的差异极显著(P<0.001), 主成分分析表明Cd、Cu和Zn是反应重金属污染的主要指标。在重金属胁迫下, 4个重金属污染区与保护区的拟水狼蛛头胸甲宽、雌体重量、卵袋重量、卵数目和卵体积差异均达到极显著(P<0.001)或显著水平(P<0.05), 主成分分析表明背胸甲宽、卵袋重量和卵体积是重要生物学指标。 拟水狼蛛的背胸甲宽、卵袋重量和卵的体积与重金属含量显著相关, 重金属含量高的地方拟水狼蛛背胸甲宽较小, 雌性体重、卵袋重量和卵数目较小, 卵的体积大, 因此说明拟水狼蛛处在逆环境中时, 动物为保持总的繁殖力不变的前提下, 后代个体的大小与数目间满足某种平衡, 即达到协同进化的目的, 拟水狼蛛可以作为重金属污染的监测指示生物。  相似文献   

7.
为了明确重金属Cd2+对拟水狼蛛Pirata subpiraticus体内金属硫蛋白(metallothionein, MT)含量及其生长发育的影响, 在室内条件下用5个不同浓度(0, 10, 20, 40和80 μg/g)的Cd2+培养的黑腹果蝇Drosophila melanogaster饲喂从5种不同生境下(S1, S2, S3, S4和S5)采集的拟水狼蛛性成熟雌蛛产卵孵化的幼蛛,待幼蛛性成熟后取所得雌蛛成蛛采用原子吸收光谱法测定了Cd2+ 诱导下拟水狼蛛体内金属硫蛋白含量及其存活率和生长率。结果表明: 食物中过量的Cd2+能够通过食物链进行传递并在拟水狼蛛体内积累, 积累量随黑腹果蝇培养基中Cd2+浓度的增加而增加,存在显著的浓度-效应关系。不同浓度的Cd2+能够诱导拟水狼蛛体内MT不同的表达,表达量与Cd2+浓度显著正相关(P<0.05)。当浓度低于20 μg/g时,污染点(S1, S2, S3和S4)拟水狼蛛体内MT表达量显著高于参照组S5(P<0.05); 当高于20 μg/g时, 所有样点拟水狼蛛体内MT表达量差异不显著(P>0.05)。拟水狼蛛存活率和成长率随着Cd2+浓度的升高呈下降趋势。据此认为,金属硫蛋白可能是蜘蛛耐受重金属污染的重要机制,与重金属具有一定浓度-效应关系。  相似文献   

8.
利用噬菌体随机十二肽库和亲和层析技术对重金属Cd进行亲和筛选,共获得两条Cd结合肽序列。将展示有Cd2+结合肽的噬菌体单克隆扩增物对不同重金属离子(Cd2+、Cr2+、Cu2+、Co2+、Zn2+、Ni2+)螯合的树脂进行亲和测定,结果表明Cu2+、Co2+、Zn2+、Ni2+<  相似文献   

9.
[目的] 探究镉吸附细菌是否能够高效固定土壤有效镉(Cd),为土壤有效Cd的微生物固定提供理论依据。[方法] 利用含Cd2+牛肉膏蛋白胨液体培养基对细菌进行Cd的耐受性测试筛选出镉抗性强的菌株;通过16S rRNA基因相似性及系统进化分析鉴定耐镉细菌,将菌细胞加入含CdCl2溶液中进行Cd2+吸附效率测定;通过土培模拟实验,测定土壤pH、碱解氮、有效磷、速效钾、有机质、CEC、有效Cd及微生物数量来分析镉吸附细菌对镉污染土壤的影响。[结果] 从德阳鱼腥草根际土壤中分离获得的57株细菌对Cd2+表现出不同程度的抗性,并从中筛选出3株耐Cd优势细菌普罗威登斯菌属(Providencia)DY8、芽孢杆菌属(Bacillus)DY3和芽孢杆菌属(Bacillus)DY1-4。其对溶液中的Cd2+表现出较好的吸附作用,吸附效率随着Cd2+浓度升高而降低。DY8、DY3、DY1-4能使镉污染土壤中有效Cd含量分别降低72.11%、68.55%、62.32%,同时显著提高镉污染土壤中碱解氮、有效磷的含量。[结论] Cd污染农田土壤中含有丰富的耐Cd微生物资源,Cd吸附细菌能降低土壤中有效Cd的含量,且能有效改善土壤养分条件。  相似文献   

10.
真水狼蛛的抗逆力研究   总被引:1,自引:1,他引:0  
彭宇  胡萃 《蛛形学报》1999,8(2):72-75
研究不同龄期真水狼蛛的抗逆力,并以3龄幼蛛为对象,研究了温度和食物对抗逆力的影响。结果表明:真水狼蛛的耐旱力很差,在无水的情况下,无论有无食物,均在2d之内死亡。其耐饥力随着龄期的增长而增加;雌蛛的耐饥力大于雄蛛。在23~35℃温度范围内,3龄幼蛛的耐饥力与温度呈明显的负相关关系。喂混合食物时,3龄幼蛛的耐饥力最强。温度和食物对耐旱力的影响不大。  相似文献   

11.
张征田  庞振凌  夏敏  梁子安  杜瑞卿  彭宇 《生态学报》2011,31(16):4579-4585
为探讨镉(Cd)对机体抗氧化功能及金属硫蛋白(MT)的影响,在室内分别用不添加Cd2 和添加浓度为20 mgkg-1 Cd2 培养基培养的黑腹果蝇来饲喂4种不同生境下(S1,S2,S3和S4)拟水狼蛛,于饲喂5d、10d和20d后,分别测定其体内MT和丙二醛 (MDA)的含量及超抗氧化酶(GST、SOD和CAT)活性。结果表明:1,不同生境拟水狼蛛用不添加Cd2 培养基培养的黑腹果蝇饲喂后,不添加Cd2 对照组拟水狼蛛镉的积累量和MT含量无显著变化,但均显著低于添加Cd2 污染组。添加Cd2 污染组拟水狼蛛镉的积累量和MT含量都显著高于对照组,且均随着饲喂时间的延长而显著升高,具有明显的时间–效应关系(p<0.05)。2,在饲喂5d和10d后,不添加Cd饲喂的拟水狼蛛MDA含量和抗氧化酶系差异都不显著。添加Cd2 污染组(S1,S2和S3)MDA含量显著高于对照组(S4),MDA含量与饲喂时间呈显著正相关(p<0.05);GST、SOD和CAT等抗氧化酶活性污染组显著低于对照组,与饲喂时间呈显著负相关(p<0.05);饲喂20d后,污染组MDA含量和抗氧化酶(SOD和CAT)活性均与对照组无显著差异,但GST活性差异显著。  相似文献   

12.
Cadmium uptake, tissue localization and structural changes induced at cellular level are essential to understand Cd tolerance in plants. In this study we have exposed plants of Pteris vittata to different concentrations of CdCl2 (0, 30, 60, 100 μM) to evaluate the tolerance of the fern to cadmium. Cadmium content determination and its histochemical localization showed that P. vittata not only takes up, but also transports and accumulates cadmium in the aboveground tissues, delocalizing it mainly in the less bioactive tissues of the frond, the trichomes and the scales. Cadmium tolerance in P. vittata was strictly related to morphogenic response induced by the metal itself in the root system. Adaptive response regarded changes of the root apex size, the developmental pattern of root hairs, the differentiation of xylem elements and endodermal suberin lamellae. All the considered parameters suggest that, in our experimental conditions, 60 μM of Cd may represent the highest concentration that P. vittata can tolerate; indeed this Cd level even improves the absorbance features of the root and allows good transport and accumulation of the metal in the fronds. The results of this study can provide useful information for phytoremediation strategies of soils contaminated by Cd, exploiting the established ability of P. vittata to transport, delocalize in the aboveground biomass and accumulate polluting metals.  相似文献   

13.
为了探明重金属在拟水狼蛛Pirata subpiraticus体内的分布及对其体内抗氧化酶活性的影响,本研究于2009年6月在河南南阳地区5种不同生境下,共采集50份土壤样本和300头拟水狼蛛样本,采用原子吸收光谱法测定了5种不同生境下雄雌拟水狼蛛体内重金属的分布、GSH含量以及GST,CAT和SOD的活性。结果表明:5个采集样点(S1, S2, S3, S4和S5) 拟水狼蛛体内重金属(Cd,Pb,Cu和Zn)的含量差异显著(P<0.05)。同一地点拟水狼蛛体内重金属(Cd,Pb,Cu和Zn)的含量不同身体部位差异显著(P<0.05),头胸部>足部>腹部,同一地点雄性拟水狼蛛重金属含量显著高于雌性(P<0.05)。在重金属胁迫下,重金属含量高(S1,S2,S3和S4)的拟水狼蛛体内GSH 含量显著高于参照组(S5),雄性GSH 含量高于雌性(P<0.05)。对于不同身体部位,GSH 含量差异显著,头胸部GSH 含量最高,其次为足部,腹部含量最低;GSH 含量与重金属含量显著正相关(r2=0.9854,P<0.05)。对于GST,CAT和SOD,重金属含量高则酶活性低,雄性酶活性显著低于雌性,不同身体部位酶活差异不显著;GST,CAT和SOD酶活性与重金属(Cd和Pb或者Pb)含量显著负相关。因此检测拟水狼蛛不同身体部位的酶活性变化就可知环境中重金属的污染程度,拟水狼蛛可以作为重金属污染的重要监测指示生物。  相似文献   

14.
Ganga is the largest riverine system of India with a fragile ecosystem. Its prone to anthropogenic disturbances because of its cultural, economic and environmental values. The contamination of river Ganga by heavy metals (HM) is due to biotic (anthropogenic sources) and abiotic (pesticides, fertilizers) sources that poses a devastating health hazard to human, plant and edible fish life. The chemical analysis with the help of atomic absorption spectrometer performed on its water samples demonstrated the accumulation of heavy metals such as Arsenic (As), Lead (Pb), Cadmium (Cd), Iron (Fe), Zinc (Zn). Moreover, the spectrophotometric analysis indicated clearly the accumulation of heavy metals in order of occurrence (Fe > As > Cd > Zn > Pb) in liver and (Zn > Fe > As > Cd > Pb) in kidney of edible fish Channa punctatus. The present study has be used to sensitively monitor the extent of heavy metals pollution in the biotic aqua life of river Ramganga system and its suggested that the bioaccumulation of heavy metal in Channa punctatus has reached above permissible limits for human consumption, indicating potential health risks. Necessary biological steps should be taken to handle such food pollution and prevent the environmental risk and food chain disruption.  相似文献   

15.
The assimilation of trace metals from food can be the main route of metal uptake into aquatic invertebrates. The assimilation efficiencies of zinc and cadmium from muscle tissue of a cephalopod mollusc (40% to 70% Zn, 64% to 83% Cd) and from a macrophytic alga (50% to 69% Zn, 39% to 50% Cd) were measured in juvenile penaeid prawns Penaeus indicus. Assimilated Zn and Cd were retained mostly in the hepatopancreas of the prawns, some metal being excreted (Zn ke 0.10 and 0.11 and Cd ke 0.004 and 0.009, from cephalopod muscle and alga, respectively). There were no significant differences between kes (efflux rate constants) of one metal from either diet. Given the high trace metal assimilation efficiencies measured here, it is highly probable that metal assimilation from food plays an important role in Zn and Cd accumulation in the body of P. indicus, particularly in estuarine stages of the life cycle, for estuaries are particularly prone to metal pollution and are likely to offer the prawns abundant metal-rich diets such as detrital material derived from local macrophytes.  相似文献   

16.
Heavy metal pollution of agricultural soils is one of the most severe ecological problems in the world. Prosopis juliflora, a phreatophytic tree species, grows well in heavy metal laden industrial sites and is known to accumulate heavy metals. Heavy Metal ATPases (HMAs) are ATP driven heavy metal pumps that translocate heavy metals across biological membranes thus helping the plant in heavy metal tolerance and phytoremediation. In the present study we have isolated and characterized a novel 28.9 kDa heavy metal ATPase peptide (PjHMT) from P. juliflora which shows high similarity to the C-terminal region of P1B ATPase HMA1. It also shows the absence of the invariant signature sequence DKTGT, and the metal binding CPX motif but the presence of conserved regions like MVGEGINDAPAL (ATP binding consensus sequence), HEGGTLLVCLNS (metal binding domain) and MLTGD, GEGIND and HEGG motifs which play important roles in metal transport or ATP binding. PjHMT, was found to be upregulated under cadmium and zinc stress. Heterologous expression of PjHMT in yeast showed a higher accumulation and tolerance of heavy metals in yeast. Further, transgenic tobacco plants constitutively expressing PjHMT also showed increased accumulation and tolerance to cadmium. Thus, this study suggests that the transport peptide from P. juliflora may have an important role in Cd uptake and thus in phytoremediation.  相似文献   

17.
18.
A limiting factor in land application of sewage sludge is the resultant heavy metal accumulation in soils followed by biomagnification in the food chain, posing a potential hazard to animal and human health. In view of this fact, pot experiments were conducted to evaluate the effect of digested sludge application to soil on phytotoxicity of heavy metals such as Cd, Cr, Ni, and Pb to radish (Raphanus sativus L.) plants. Increasing sludge levels resulted in increased levels of DTPA-extractable heavy metals in the soil. Cadmium was the dominant metal extracted by DTPA followed by Ni, Pb, and Cr. The extractability of metals by DTPA tended to decrease from the first to the second crop. Dry matter yield of radish increased significantly as a function of increasing sludge treatments. Soil application of sludge raised the concentration of one or more heavy metals in plants. Shoots contained higher concentrations of Cd, Cr, and Ni than the roots of radish plants. Shoot concentrations of Cd, Cr, Ni, and Pb were within the tolerance levels of this crop at all rates of sludge application. Shoot as well as root concentration of Cd was above 0.5 mg kg?1, considered toxic for human and animal consumption. The levels of DTPA-extractable Cd and Ni were less correlated while those of Cr and Pb were more correlated with their respective shoot and root contents. The results emphasize that accumulation of potentially toxic heavy metals in soil and their build-up in vegetable crops should not be ignored when sludge is applied as an amendment to land.  相似文献   

19.
成都平原北部水稻土重金属含量状况及其潜在生态风险评价   总被引:13,自引:0,他引:13  
秦鱼生  喻华  冯文强  王正银  涂仕华 《生态学报》2013,33(19):6335-6344
为了解成都平原水稻土重金属含量状况和潜在的生态风险,选取成都平原北部水稻土典型区域为研究对象,采集了158个表层土壤样品,分析了土壤中pH值和Cd、Cu、As、Hg、Pb、Cr、Ni 7种重金属元素含量,以20世纪80年代测定的成都平原土壤重金属元素背景值为评价标准,采用Hakanson潜在生态危害指数法对研究区域的重金属潜在生态风险进行了评价。结果表明:研究区域水稻土Cd、Hg、Ni、Cu、Pb、Cr和As平均含量分别为0.709、0.187、32.08、34.12、31.52、82.13 mg/kg和7.25 mg/kg;Cd、Ni、Cu和Hg 4种重金属超过《土壤环境质量标准》(GB15618-1995) Ⅱ级标准值样本比例分别为87.34%、8.23%、3.80%和3.80%,Cd含量超标严重。7种重金属元素变异系数幅度为18.35%-49.03%,由大到小依次为Cd、Hg、Cu、As、Ni、Cr、Pb。75.32%的样本达到中度或较强重金属潜在生态风险,区域整体表现为中度潜在生态风险(RI平均值为198.65),Cd和Hg为高生态风险元素,对潜在生态风险贡献率分别为62.27%和20.78%,As、Pb、Cu、Ni、Cr为低生态风险元素;风险概率图显示城区周边和绵远河沿线的潜在生态风险等级较高。因此,成都平原水稻土农业生产中应采取一定的措施防控农产品Cd和Hg污染。  相似文献   

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