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1.
Singh K Ghai M Garg M Chhuneja P Kaur P Schnurbusch T Keller B Dhaliwal HS 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,115(3):301-312
Diploid A genome species of wheat harbour immense variability for biotic stresses and productivity traits, and these could
be transferred efficiently to hexaploid wheat through marker assisted selection, provided the target genes are tagged at diploid
level first. Here we report an integrated molecular linkage map of A genome diploid wheat based on 93 recombinant inbred lines
(RILs) derived from Triticum boeoticum × Triticum monococcum inter sub-specific cross. The parental lines were analysed with 306 simple sequence repeat (SSR) and 194 RFLP markers, including
66 bin mapped ESTs. Out of 306 SSRs tested for polymorphism, 74 (24.2%) did not show amplification (null) in both the parents.
Overall, 171 (73.7%) of the 232 remaining SSR and 98 (50.5%) of the 194 RFLP markers were polymorphic. Both A and D genome
specific SSR markers showed similar transferability to A genome of diploid wheat species. The 176 polymorphic markers, that
were assayed on a set of 93 RILs, yielded 188 polymorphic loci and 177 of these as well as two additional morphological traits
mapped on seven linkage groups with a total map length of 1,262 cM, which is longer than most of the available A genome linkage
maps in diploid and hexaploid wheat. About 58 loci showed distorted segregation with majority of these mapping on chromosome
2Am. With a few exceptions, the position and order of the markers was similar to the ones in other maps of the wheat A genome.
Chromosome 1Am of T. monococcum and T. boeoticum showed a small paracentric inversion relative to the A genome of hexaploid wheat. The described linkage map could be useful
for gene tagging, marker assisted gene introgression from diploid into hexaploid wheat as well as for map based cloning of
genes from diploid A genome species and orthologous genes from hexaploid wheat. 相似文献
2.
SSR-based linkage map with new markers using an intraspecific population of common wheat 总被引:14,自引:0,他引:14
Torada A Koike M Mochida K Ogihara Y 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,112(6):1042-1051
Simple sequence repeats (SSRs) are valuable molecular markers in many plant species. In common wheat (Triticum aestivum L.), which is characteristic of its large genomes and alloploidy, SSRs are one of the most useful markers. To increase SSR
marker sources and construct an SSR-based linkage map of appropriate density, we tried to develop new SSR markers from SSR-enriched
genomic libraries and the public database. SSRs having (GA)n and (GT)n motifs were isolated from enriched libraries, and di-
and tri-nucleotide repeats were mined from expressed sequence tags (ESTs) and DNA sequences of Triticum species in the public database. Of the 1,147 primer pairs designed, 842 primers gave accurate amplification products, and
478 primers showed polymorphism among the nine wheat lines examined. Using a doubled haploid (DH) population from an intraspecific
cross between Kitamoe and Münstertaler (KM), we constructed an SSR-based linkage map that consisted of 464 loci: 185 loci
from genomic libraries, 65 loci from the sequence database including ESTs, 213 loci from the SSR markers already reported,
and 1 locus of morphological marker. Although newly developed SSR loci were distributed throughout all chromosomes, clustering
of them around putative centromeric regions was found on several chromosomes. The total length of the KM map spanned 3,441 cM
and corresponded to approximately 86% genome coverage. The KM map comprised of 23 linkage groups because two gaps of over
50 cM distance remained on chromosome 6A. This is a first report of SSR-based linkage map using single intraspecific population
of common wheat. This mapping result suggests that it becomes possible to construct linkage maps with sufficient genome coverage
using only SSR markers without RFLP markers, even in an intraspecific population of common wheat. Moreover, the new SSR markers
will contribute to the enrichment of molecular marker resources in common wheat. 相似文献
3.
Ulloa M Meredith WR Shappley ZW Kahler AL 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(2-3):200-208
An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 相似文献
4.
Han OK Kaga A Isemura T Wang XW Tomooka N Vaughan DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1278-1287
To make progress in genome analysis of azuki bean (Vigna angularis) a genetic linkage map was constructed from a backcross population of (V. nepalensis x V. angularis) x V.angularis consisting of 187 individuals. A total of 486 markers—205 simple sequence repeats (SSRs), 187 amplified fragment length polymorphisms
(AFLPs) and 94 restriction fragment length polymorphisms (RFLPs) —were mapped onto 11 linkage groups corresponding to the
haploid chromosome number of azuki bean. This map spans a total length of 832.1 cM with an average marker distance of 1.85 cM
and is the most saturated map for a Vigna species to date. In addition, RFLP markers from other legumes facilitated finding several orthologous linkage groups based
on previously published RFLP linkage maps. Most SSR primers that have been developed from SSR-enriched libraries detected
a single locus. The SSR loci identified are distributed throughout the azuki bean genome. This moderately dense linkage map
equipped with many SSR markers will be useful for mapping a range of useful traits such as those related to domestication
and stress resistance. The mapping population will be used to develop advanced backcross lines for high resolution QTL mapping
of these traits.
O.K. Han, A. Kaga, T. Isemura have contributed equally to this paper. 相似文献
5.
Amine Zraidi Gertraud Stift Martin Pachner Abdolali Shojaeiyan Li Gong Tamas Lelley 《Molecular breeding : new strategies in plant improvement》2007,20(4):375-388
Using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSR),
and morphological traits, the first genetic maps for Cucurbita pepo (2n=2x=40) were constructed and compared. The two mapping populations consisted of 92 F2 individuals each. One map was developed from a cross between an oil-seed pumpkin breeding line and a zucchini accession,
into which genes for resistance to Zucchini Yellow Mosaic Virus (ZYMV) from a related species, C. moschata, had been introgressed. The other map was developed from a cross between an oil-seed pumpkin and a crookneck variety. A total
of 332 and 323 markers were mapped in the two populations. Markers were distributed in each map over 21 linkage groups and
covered an average of 2,200 cM of the C. pepo genome. The two maps had 62 loci in common, which enabled identification of 14 homologous linkage groups. Polyacrylamide
gel analyses allowed detection of a high number of markers suitable for mapping, 10% of which were co-dominant RAPD loci.
In the Pumpkin-Zucchini population, bulked segregant analysis (BSA) identified seven markers less than 7 cM distant from the
locus n, affecting lignification of the seed coat. One of these markers, linked to the recessive hull-less allele (AW11-420), was
also found in the Pumpkin-Crookneck population, 4 cM from n. In the Pumpkin-Zucchini population, 24 RAPD markers, previously introduced into C. pepo from C. moschata, were mapped in two linkage groups (13 and 11 markers in LGpz1 and LGpz2, respectively), together with two sequence characterized
amplified region (SCAR) markers linked to genes for resistance to ZYMV. 相似文献
6.
Linkages between restriction fragment length,isozyme, and morphological markers in lentil 总被引:1,自引:0,他引:1
M. J. Havey F. J. Muehlbauer 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1989,77(3):395-401
Summary A genetic linkage map of lentil comprising 333 centimorgans (cM) was constructed from 20 restriction fragment length, 8 isozyme, and 6 morphological markers segregating in a single interspecific cross (Lens culinaris × L. orientalis). Because the genotypes at marker loci were determined for about 66 F2 plants, linkages are only reported for estimates of recombination less than 30 cM. Probes for identification of restriction fragment length polymorphisms (RFLPs) were isolated from a cDNA and EcoRI and PstI partial genomic libraries of lentil. The cDNA library gave the highest frequency of relatively low-copy-number probes. The cDNAs were about twice as efficient, relative to random genomic fragments, in RFLP detection per probe. Nine markers showed significant deviations from the expected F2 ratios and tended to show a predominance of alleles from the cultigen. Assuming a genome size of 10 Morgans, 50% of the lentil genome could be linked within 10 cM of the 34 markers and the map is of sufficient size to attempt mapping of quantitative trait loci. 相似文献
7.
An integrated DArT-SSR linkage map of durum wheat 总被引:2,自引:0,他引:2
Paola Mantovani Marco Maccaferri Maria Corinna Sanguineti Roberto Tuberosa Ilaria Catizone Peter Wenzl Brent Thomson Jason Carling Eric Huttner Enzo DeAmbrogio Andrzej Kilian 《Molecular breeding : new strategies in plant improvement》2008,22(4):629-648
Genetic mapping in durum wheat (Triticum durum Desf.) is constrained by its large genome and allopolyploid nature. We developed a Diversity Arrays Technology (DArT) platform
for durum wheat to enable efficient and cost-effective mapping and molecular breeding applications. Genomic representations
from 56 durum accessions were used to assemble a DArT genotyping microarray. Microsatellite (SSR) and DArT markers were mapped
on a durum wheat recombinant inbred population (176 lines). The integrated DArT-SSR map included 554 loci (162 SSRs and 392
DArT markers) and spanned 2022 cM (5 cM/marker on average). The DArT markers from durum wheat were positioned in respect to
anchor SSRs and hexaploid wheat DArT markers. DArT markers compared favourably to SSRs to evaluate genetic relationships among
the durum panel, with 1315 DArT polymorphisms found across the accessions. Combining DArT and SSR platforms provides an efficient
and rapid method of generating linkage maps in durum wheat. 相似文献
8.
P. Hernández G. Dorado P. Prieto M. J. Giménez M. C. Ramírez D. A. Laurie J. W. Snape A. Martín 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(8):1259-1264
The first genetic map of the wild South Ameri- can barley species Hordeum chilense is presented. The map, based on an F2 population of 114 plants, contains 123 markers, including 82 RAPDs, 13 SSRs, 16 RFLPs, four SCARs, two seed storage proteins
and two STS markers. The map spans 694 cM with an average distance of 5.7 cM between markers. Six additional SSRs and seven
additional SCARs which were not polymorphic were assigned to chromosomes using wheat/H. chilense addition lines. Polymorphisms were revealed by 50% of the RAPD amplifications, 13% of wheat and barley SSR primers, and 78%
of the Gramineae RFLP anchor probes. The utility of SSR and RFLP probes from other Gramineae species shows the usefulness
of a comparative approach as a source of markers and for aligning the genetic map of H. chilense with other species. This also indicates that the overall structure of the H. chilense linkage groups is probably similar to that of the B and D genomes of wheat and the H genome of barley. Applications of the
map for tritordeum and wheat breeding are discussed.
Received: 20 August 2000 / Accepted: 22 September 2000 相似文献
9.
D. Crouzillat E. Lerceteau V. Petiard J. Morera H. Rodriguez D. Walker W. Phillips C. Ronning R. Schnell J. Osei P. Fritz 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(1-2):205-214
A genetic linkage map of Theobroma cacao (cocoa) has been constructed from 131 backcross trees derived from a cross between a single tree of the variety Catongo and an F1 tree from the cross of Catongo by Pound 12. The map comprises 138 markers: 104 RAPD loci, 32 RFLP loci and two morphologic loci. Ten linkage groups were found which cover 1068 centimorgans (cM). Only six (4%) molecular-marker loci show a significant deviation from the expected 11 segregation ratio.The average distance between two adjacent markers is 8.3 cM. The final genome-size estimates based on two-point linkage data ranged from 1078 to 1112 cM for the cocoa genome. This backcross progeny segregates for two apparently single gene loci controlling (1) anthocyanidin synthesis (Anth) in seeds, leaves and flowers and (2) self-compatibility (Autoc). The Anth locus was found to be 25 cM from Autoc and two molecular markers co-segregate with Anth. The genetic linkage map was used to localize QTLs for early flowering, trunk diameter, jorquette height and ovule number in the BC1 generation using both single-point ANOVA and interval mapping. A minimum number of 2–4 QTLs (P<0.01) involved in the genetic expression of the traits studied was detected. Coincident map locations of a QTL for jorquette height and trunk diameter suggests the possibility of pleiotropic effects in cocoa for these traits. The combined estimated effects of the different mapped QTLs explained between 11.2% and 25.8% of the phenotypic variance observed in the BC1 population. 相似文献
10.
The primary genetic linkage maps of Fenneropenaeus
chinensis (Osbeck) were constructed by using the “two-way pseudo-testcross” strategy with RAPD and SSR markers. Parents and F1 progeny
were used as segregating populations. Sixty-one RAPD primers and 20 pairs of SSR primers were screened from 460 RAPD primers
and 44 pairs of SSR primers. These primers were used to analyze the parents and 82 progeny of the mapping family. About 146
primers (128 RAPDs, 18 microsatellites) in the female and 127 primers (109 RAPDs, 18 microsatellites) in the male were segregating
markers. The female linkage map included eight linkage groups, nine triplets and 14 doublets, spanning 1,173 cM with the average
marker density of 11.28 cM, and the observed coverage was 59.36%. The male linkage map included 10 linkage groups, 12 triplets
and seven doublets, spanning 1,144.6 cM with the average marker density of 12.05 cM, and the observed coverage was 62.01%.
The construction of the F. chinensis genetic linkage maps here opened a new prospect for marker-assisted selection program, comparative genomics and quantitative
trait loci (QTL) gene location and cloning. 相似文献
11.
Manli Li Nana Yuyama Mariko Hirata Yunwen Wang Jianguo Han Hongwei Cai 《Molecular breeding : new strategies in plant improvement》2010,26(3):467-476
Japanese lawngrass (Zoysia japonica) and Manila grass (Z. matrella) are the two most important and commonly used Zoysia species. A consensus based SSR linkage map was developed for the genus by combining maps from each species. This used previously
constructed maps for two Z. japonica populations and a new map from Z. matrella. The new SSR linkage map for Z. matrella was based on 86 F2 individuals and contained 213 loci and covered a map distance of 1,351.2 cM in 32 linkage groups. Comparison of the three
linkage maps constructed from populations with different genetic backgrounds indicated that most markers exhibited a consensus
order, although some intervals or regions displayed discrepancy in marker orders or positions. The integrated map comprises
507 loci with a mean interval of 4.1 cM, covering a map distance of 2,066.6 cM in 22 linkage groups. The SSR-based map will
allow marker-assisted selection and be useful for the mapping and cloning of economically important genes or quantitative
trait loci. 相似文献
12.
Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny 总被引:4,自引:0,他引:4
N. Foisset R. Delourme P. Barret N. Hubert B. S. Landry M. Renard 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1996,93(7):1017-1025
We have undertaken the construction of a Brassica napus genetic map with isozyme (4%), RFLP (26.5%) and RAPD (68%) markers on a 152 lines of a doubled-haploid population. The map covers 1765 cM and comprises 254 markers including three PCR-specific markers and a morphological marker. They are assembled into 19 linkage groups, covering approximatively 71% of the rapeseed genome. Thirty five percent of the studied markers did not segregate according to the expected Mendelian ratio and tended to cluster in eight specific linkage groups. In this paper, the structure of the genetic map is described and the existence of non-Mendelian segregations in linkage analysis as well as the origins of the observed distortions, are discussed. The mapped RFLP loci corresponded to the cDNAs already used to construct B. napus maps. The first results of intraspecific comparative mapping are presented. 相似文献
13.
Chaitieng B Kaga A Tomooka N Isemura T Kuroda Y Vaughan DA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2006,113(7):1261-1269
The Asian Vigna group of grain legumes consists of six domesticated species, among them black gram is widely grown in South Asia and to a lesser extent in Southeast Asia. We report the first genetic linkage map of black gram [Vigna mungo (L.) Hepper], constructed using a BC1F1 population consisting of 180 individuals. The BC1F1 population was analyzed in 61 SSR primer pairs, 56 RFLP probes, 27 AFLP loci and 1 morphological marker. About 148 marker loci could be assigned to the 11 linkage groups, which correspond to the haploid chromosome number of black gram. The linkage groups cover a total of 783 cM of the black gram genome. The number of markers per linkage group ranges from 6 to 23. The average distance between adjacent markers varied from 3.5 to 9.3 cM. The results of comparative genome mapping between black gram and azuki bean show that the linkage order of markers is highly conserved. However, inversions, insertions, deletions/duplications and a translocation were detected between the black gram and azuki bean linkage maps. The marker order on parts of linkage groups 1, 2 and 5 is reversed between the two species. One region on black gram linkage group 10 appears to correspond to part of azuki bean linkage group 1. The present study suggests that the azuki bean SSR markers can be widely used for Asian Vigna species and the black gram genetic linkage map will assist in improvement of this crop.Electronic Supplementary Material Supplementary material is available in the online version of this article at and is accessible for authorized users.The first three authors contributed equally to this research 相似文献
14.
Watanabe T Fujita H Yamasaki K Seki S Taniguchi N 《Marine biotechnology (New York, N.Y.)》2004,6(4):327-334
A mapping referential family (F1) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map. 相似文献
15.
Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population 总被引:26,自引:8,他引:18
J. M. Costa A. Corey P. M. Hayes C. Jobet A. Kleinhofs A. Kopisch-Obusch S. F. Kramer D. Kudrna M. Li O. Riera-Lizarazu K. Sato P. Szucs T. Toojinda M.I. Vales R. I. Wolfe 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):415-424
A phenotypically polymorphic barley (Hordeum vulgare L.) mapping population was developed using morphological marker stocks as parents. Ninety-four doubled-haploid lines were
derived for genetic mapping from an F1 using the Hordeum bulbosum system. A linkage map was constructed using 12 morphological markers, 87 restriction fragment length polymorphism (RFLP),
five random amplified polymorphic DNA (RAPD), one sequence-tagged site (STS), one intron fragment length polymorphism (IFLP),
33 simple sequence repeat (SSR), and 586 amplified fragment length polymorphism (AFLP) markers. The genetic map spanned 1,387
cM with an average density of one marker every 1.9 cM. AFLP markers tended to cluster on centromeric regions and were more
abundant on chromosome 1 (7H). RAPD markers showed a high level of segregation distortion, 54% compared with the 26% observed
for AFLP markers, 27% for SSR markers, and 18% for RFLP markers. Three major regions of segregation distortion, based on RFLP
and morphological markers, were located on chromosomes 2 (2H), 3 (3H), and 7 (5H). Segregation distortion may indicate that
preferential gametic selection occurred during the development of the doubled-haploid lines. This may be due to the extreme
phenotypes determined by alleles at morphological trait loci of the dominant and recessive parental stocks. Several molecular
markers were found to be closely linked to morphological loci. The linkage map reported herein will be useful in integrating
data on quantitative traits with morphological variants and should aid in map-based cloning of genes controlling morphological
traits.
Received: 23 August 2000 / Accepted: 15 December 2000 相似文献
16.
Construction of an RFLP linkage map for cultivated sunflower 总被引:5,自引:0,他引:5
C. C. Jan B. A. Vick J. F. Miller A. L. Kahler E. T. Butler III. 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):15-22
An RFLP linkage map was constructed for cultivated sunflower Helianthus annuus L., based on 271 loci detected by 232 cDNA probes. Ninety-three F2 plants of a cross between inbred lines RHA 271 and HA 234 were used as the mapping population. These genetic markers plus
a fertility restoration gene, Rf
1, defined 20 linkage groups, covering 1164 cM of the sunflower genome. Of the 71 loci 202 had codominant genotypic segregation,
with the rest showing dominant segregation. Thirty-two of the 232 probes gave multiple locus segregation. There were 39 clusters
of tightly linked markers with 0 cM distance among loci. This map has an average marker-to-marker distance of 4.6 cM, with
11 markerless regions exceeding 20 cM.
Received: 17 June 1997 / Accepted: 19 June 1997 相似文献
17.
M. J. Truco C. F. Quiros 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1994,89(5):590-598
We constructed a genetic map on Brassica nigra based on a segregating population of 83 F2 individuals. Three different types of molecular markers were used to build the map including isozymes, restriction fragment length polymorphisms (RFLP), and random amplified polymorphic DNA (RAPD). The final map contained 124 markers distributed in 11 linkage groups. The map covered a total distance of 677 cM with the markers distributed within a mean distance of 5.5cM. Of the sequences found in the B. nigra map, 40% were duplicated and organized into three different types of arrangements. They were either scattered throughout the genome, organized in tandem, or organized in blocks of duplicated loci conserved in more than 1 linkage group. 相似文献
18.
Isobe S Klimenko I Ivashuta S Gau M Kozlov NN 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2003,108(1):105-112
We constructed a genetic linkage map of red clover (Trifolium pratense L., 2n=2x=14) using RFLP markers from cDNA probes of a backcrossed mapping population, and investigated the transferability of the markers to other red clover germplasm. The map contains 157 RFLP markers and one morphological marker on seven linkage groups. The total map distance was 535.7 cM and the average distance between two markers was 3.4 cM. All of the cDNA probes of the map were hybridized to the fragments of genomic DNA from 12 plants derived from three varieties, and 87% of the cDNA probes detected polymorphic bands that corresponded to those of mapping parents. This result indicated that RFLP markers on the present map were transferable to the genome analysis of other red clover germplasm. This is the first report to construct a linkage map of Trifolium species; it should provide fundamental and useful genetic information relevant to the breeding of red clover and genus Trifolium.Communicated by H.C. Becker 相似文献
19.
Zhang Y Sledge MK Bouton JH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2007,114(8):1367-1378
Allotetraploid white clover (Trifolium repens L.), a cool-season perennial legume used extensively as forage for livestock, is an important target for marker-assisted
breeding. A genetic linkage map of white clover was constructed using simple sequence repeat (SSR) markers based on sequences
from several Trifolieae species, including white clover, red clover (T. pratense L.), Medicago truncatula (Gaertn.) and soybean (Glycine max L.). An F1 population consisting of 179 individuals, from a cross between two highly heterozygous genotypes, GA43 and Southern Regional
Virus Resistant, was used for genetic mapping. A total of 1,571 SSR markers were screened for amplification and polymorphism
using DNA from two parents and 14 F1s of the mapping population. The map consists of 415 loci amplified from 343 SSR primer pairs, including 83 from white clover,
181 from red clover, 77 from M. truncatula, and two from soybean. Linkage groups for all eight homoeologous chromosome pairs of allotetraploid white clover were detected.
Map length was estimated at 1,877 cM with 87% genome coverage. Map density was approximately 5 cM per locus. Segregation distortion
was detected in six segments of the genome (homoeologous groups A1, A2, B1, B2, C1, and D1). A comparison of map locations
of markers originating from white clover, red clover, and alfalfa (M. sativa L.) revealed putative macro-colinearity between the three Trifolieae species. This map can be used to link quantitative trait
loci with SSR markers, and accelerate the improvement of white clover by marker-assisted selection and breeding.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
20.