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1.
将含有鸡传染性支气管炎病毒 S1 基因c D N A 的重组转移质粒p S X I V V I+ X3 S1 . Holte 和p S X I V V I+ X3/4 S1 . Holte 分别与粉纹夜蛾核型多角体病毒 Tn N P V S V I- G D N A( O C C- ,gal+ ) 共转染草地夜蛾( Sf9) 细胞,经空斑纯化得到重组病毒 Tn N P V( X3) S1 . Holte O C C+ 和 Tn N P V( X3/4) S1 . Holte O C C+ 。将重组毒株分别感染 Tn5 B1 细胞,并进行 S D S P A G E 与 Westernblot 检测。结果表明, Tn N P V( X3/4) S1 . Holte O C C+ 在感染的细胞中高效表达了 S1 蛋白, S D S P A G E 凝胶薄层色谱分析结果显示,感染病毒后72 h S1 蛋白的表达量占细胞内总蛋白量的35 .8 % ,而 Tn N P V( X3) S1 . Holte O C C+ 感染的细胞内检测不出 S1 蛋白。经分析认为这一差异主要来自 S1 基因翻译起始位点及其附近的周围环境。  相似文献   

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硫代反义寡核苷酸在细胞培养内抗甲型流感病毒活性   总被引:5,自引:0,他引:5  
陈忠斌  王升启 《病毒学报》1998,14(3):193-198
为了研究抗流感病毒特异性反义核酸药物,针对A型流感病毒基因组3'和5'端保守序列,设计并合成了4条硫代寡核苷酸(ODN):3'端反义ODN(IV3^#)与3'端正义ODN(IV3S),5'端反义ODN(IV4^#)与5'端正交ODN(IV4S)。以流感病毒血凝滴度和致细胞病变作用为指标,测定了ODNs在MDCK细胞中对A型流感病毒A/京防/86-1(H1N1)复制的影响。结果表明,与流感病毒基因组  相似文献   

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紫外线-B辐射对植物的影响研究进展   总被引:5,自引:0,他引:5  
紫外线B辐射对植物的影响研究进展①侯扶江(甘肃草原生态研究所,兰州730020)贲桂英(中国科学院西北高原生物研究所,西宁810001)ADVANCESINTHESTUDYONEFFECTSOFULTRAVIOLETBRADIATIONONPLA...  相似文献   

4.
以鸡传染性法氏囊病病毒内蒙古毒株(IBDV-NM)dsRNA为模板,用RT-PCR法扩增其主要寄主保护抗原VP2基因的全长cDNA,克隆于pUC19的XbaI/KpnI位点,进行了全序列分析。序列比较发现IBDV-NM毒株VP2基因与已报道的其它7个IBDVCJ801bkf、Cu1、PBG98、52/70、002—73、STC及VariantE毒株之间高度同源,其核苷酸序列的同源率为91.6%~96.2%,推测的氨基酸序列的同源率为96.2%~98.6%。IBDV-NM毒株VP2高变异区的第一个亲水区氨基酸序列与CJ801bkf、Cu1、PBG98、52/70、STC、002—73比较,有一个氨基酸差异,第二个亲水区氨基酸序列与上述6个毒株完全相同。而与VariantE比较,两个亲水区内各有两个氨基酸差异。此外,IBDV-NM毒株VP2具有强毒株所特有的7肽保守区:SWSASGS。这些结果表明,IBDV-NM毒株为标准血清Ⅰ型IBDV强毒株。  相似文献   

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陈训  冯芳 《云南植物研究》1997,19(4):449-450
贵州两种一变种缬草属植物染色体研究陈训1巫华美1刘朝辉1冯芳2(1贵州省生物研究所,贵阳550009)(2贵州省植物园,贵阳550001)ASTUDYONCHROMOSOMEOFTWOSPECIESANDONEVARIETIESOFVALERIANA...  相似文献   

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黄土高原地区提取植被信息方法的研究   总被引:16,自引:0,他引:16  
研究评价了适于黄土高原地区植被信息提取的最佳植被指数和方法。该地区分布有落叶阔叶林、草原和荒漠,植被类型丰富多样。然而土壤背景对植被信息提取有较大影响。经对比分析,修正后的土壤调节植被指数(MSAVI), 不仅能增强植被信号,并能大大减小土壤背景的影响,同时又能宏观地反映该地区植被类型的分布状况。它是黄土高原地区目前提取植被信息较好的植被指数。标准化差值植被指数(NDVI)、土壤调节植被指数(SAVI)和垂直植被指数(PVI)分别适用于高密度、中等密度和稀少植被地区植被信息的提取和监测。各种植被指数多时象累加产生的图像能较好地提取植被信息  相似文献   

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中国河南株丁型肝炎病毒全基因组的cDNA克隆和序列分析   总被引:3,自引:0,他引:3  
从我国河南-抗丁型肝炎病毒抗原(anti-HDAg)及丁型肝炎病毒(HDv)RNA双阳性的HBsAg携带者血清中提取RNA,采用人工合成的引物进行逆转录和聚合酶链反应(PCR),获得了贯穿HDV全基因组的6个相互重叠的cDNA片段。经双脱氧末端终止法进行核苷酸序列分析,得到了长度为1674bp的我国人河南株HDVcDNA全序列。计算机分析表明,该株与我国台湾株(HDVIA型)、美国-1株(HDVIB型)、日本-1株(HDVⅡ型)和秘鲁-1株(HDVⅢ型)的核苷酸同源性分别为的94.3%、86.8%、75.4%和66.3%,氨基酸序列的同源性分别为89.7%、85.1%、71.9%和64.6%,并在核苷酸和推导的HDAg氨基酸序列中分别发现了5个和2个集中保守的区域。这些区域均与HDV的某些重要功能密切相关。  相似文献   

8.
短尾拟鲿分类地位的探讨   总被引:3,自引:0,他引:3  
短尾拟鲿分类地位的探讨张耀光,王德寿(西南师范大学生物学系重庆630715)关键词短尾拟鲿,骨学特征,分类地位STUDIESONTHETAXONOMICALPOSITIONOFPSEUDOBAGRUSBREVICAUDATUS(BAGRIDAE,SI...  相似文献   

9.
用瞬时表达分析等方法,证明牛泡沫病毒( B S V)3026 中国毒株能在体外激活牛免疫缺陷病毒( B I V) 基因表达, B S V3026 编码的反式激活因子 Borf1 行使这种激活作用。缺失突变分析表明, Borf1 在 B I V L T R 上靶序列位于- 410/ - 115( + 1 为转录起始位点) 区域,但其中的 N Fκ B 位点( - 367/ - 319) 与这种激活作用无关,包括转录起点下游( R U5 区) 在内的- 115/ + 204 区域也与这种激活作用无关。该结果对研究 B I V 致病机理及防治 A I D S 有重要意义。  相似文献   

10.
AVHRR NDVI与气候因子的相关分析   总被引:83,自引:3,他引:80  
李本纲  陶澍 《生态学报》2000,20(5):898-902
对中国160个气象站10a的连续AVHRR NDVI数据、气象观测数据进行相关分析,并结合植被覆盖类型资料深入探讨了AVHRR NDVI/气温和AVHRR NDVI/降水相关系数的地区差异及其随植被类型变化规律。研究结果表明,对中国的大部分地区,气温对植被的影响超过降水。就自然植被而言,其对降水的敏感性趋势为草本植被大于灌木植被,灌木植被大于乔木植被。就农作物而言,降水影响取决于耕作制度、作物种类  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

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Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

20.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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