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1.
Genetic mapping and QTL analysis of fiber-related traits in cotton (<Emphasis Type="Italic">Gossypium</Emphasis>) 总被引:19,自引:0,他引:19
Mei M Syed NH Gao W Thaxton PM Smith CW Stelly DM Chen ZJ 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2004,108(2):280-291
Cotton, the leading natural fiber crop, is largely produced by two primary cultivated allotetraploid species known as Upland or American cotton (Gossypium hirsutum L.) and Pima or Egyptian cotton (G. barbadense L.). The allotetraploid species diverged from each other and from their diploid progenitors (A or D genome) through selection and domestication after polyploidization. To analyze cotton AD genomes and dissect agronomic traits, we have developed a genetic map in an F2 population derived from interspecific hybrids between G. hirsutum L. cv. Acala-44 and G. barbadense L. cv. Pima S-7. A total of 392 genetic loci, including 333 amplified fragment length polymorphisms (AFLPs), 47 simple sequence repeats (SSRs), and 12 restriction fragment length polymorphisms (RFLPs), were mapped in 42 linkage groups, which span 3,287 cM and cover approximately 70% of the genome. Using chromosomal aneuploid interspecific hybrids and a set of 29 RFLP and SSR framework markers, we assigned 19 linkage groups involving 223 loci to 12 chromosomes. Comparing four pairs of homoeologous chromosomes, we found that with one exception linkage distances in the A-subgenome chromosomes were larger than those in their D-subgenome homoeologues, reflecting higher recombination frequencies and/or larger chromosomes in the A subgenome. Segregation distortion was observed in 30 out of 392 loci mapped in cotton. Moreover, approximately 29% of the RFLPs behaved as dominant loci, which may result from rapid genomic changes. The cotton genetic map was used for quantitative trait loci (QTL) analysis using composite interval mapping and permutation tests. We detected seven QTLs for six fiber-related traits; five of these were distributed among A-subgenome chromosomes, the genome donor of fiber traits. The detection of QTLs in both the A subgenome in this study and the D subgenome in a previous study suggests that fiber-related traits are controlled by the genes in homoeologous genomes, which are subjected to selection and domestication. Some chromosomes contain clusters of QTLs and presumably contribute to the large amount of phenotypic variation that is present for fiber-related traits.Communicated by J. Dvorak 相似文献
2.
A genetic linkage map of durum wheat 总被引:20,自引:6,他引:14
A. Blanco M. P. Bellomo A. Cenci C. De Giovanni R. D’Ovidio E. Iacono B. Laddomada M. A. Pagnotta E. Porceddu A. Sciancalepore R. Simeone O. A. Tanzarella 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):721-728
A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from
a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR
(polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the
Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total
of 213 loci were mapped at a LOD≥3 on all 14 chromosomes of the A and B genomes. The total length of the map is 1352 cM and
the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD≥3. A fraction (18.6%)
of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were
found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using
several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving
chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful
tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for
studies of genome organisation in small grain cereal species.
Received: 5 January 1998 / Accepted: 31 March 1998 相似文献
3.
Guohua Yang Sansi Tu Shaoqing Li Lingling Feng Jin Kong Hui Li Yangsheng Li 《Frontiers of Biology in China》2008,3(4):443-448
A population of 117 doubled haploid (DH) lines derived from the cross of Zhaiyeqing 8 (indica)× Jingxi 17 (japonica) was employed to map quantitative trait loci (QTL) underlying four physiological traits related to chlorophyll contents of
the flag leaf. There were significantly positive correlations among chlorophyll a, chlorophyll b and chlorophyll a + b content.
Chlorophyll a/b ratio was significantly negatively correlated with chlorophyll b content. These four traits were normally
distributed with transgressive segregation, suggesting that they were controlled by multiple minor genes. A total of 11 QTLs
were detected for the four traits and they lay on six chromosomes. Each of them explained 9.2%–19.6% of the phenotypic variations,
respectively. Of these, two QTLs controlling chlorophyll a content were mapped on chromosomes 2 and 5; four QTLs underlying
chlorophyll b content were mapped on chromosomes 2, 3, 5 and 9; three QTLs underlying chlorophyll a + b amount were mapped
on chromosomes 3, 5 and 9; two QTLs underlying chlorophyll a/b ratio were mapped on chromosomes 6 and 11. The intrinsic relationship
among the four traits and the practical implication in rice breeding are discussed.
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Translated from Journal of Wuhan University (Science Edition), 2006, 52(6): 751–756 [译自: 武汉大学学报(理学版)] 相似文献
4.
Genetic mapping of new cotton fiber loci using EST-derived microsatellites in an interspecific recombinant inbred line cotton population 总被引:11,自引:0,他引:11
Park YH Alabady MS Ulloa M Sickler B Wilkins TA Yu J Stelly DM Kohel RJ el-Shihy OM Cantrell RG 《Molecular genetics and genomics : MGG》2005,274(4):428-441
There is an immediate need for a high-density genetic map of cotton anchored with fiber genes to facilitate marker-assisted
selection (MAS) for improved fiber traits. With this goal in mind, genetic mapping with a new set of microsatellite markers
[comprising both simple (SSR) and complex (CSR) sequence repeat markers] was performed on 183 recombinant inbred lines (RILs)
developed from the progeny of the interspecific cross Gossypium hirsutum L. cv. TM1 × Gossypium barbadense L. Pima 3-79. Microsatellite markers were developed using 1557 ESTs-containing SSRs (≥10 bp) and 5794 EST-containing CSRs
(≥12 bp) obtained from ~14,000 consensus sequences derived from fiber ESTs generated from the cultivated diploid species Gossypium arboreum L. cv AKA8401. From a total of 1232 EST-derived SSR (MUSS) and CSR (MUCS) primer-pairs, 1019 (83%) successfully amplified
PCR products from a survey panel of six Gossypium species; 202 (19.8%) were polymorphic between the G. hirsutum L. and G. barbadense L. parents of the interspecific mapping population. Among these polymorphic markers, only 86 (42.6%) showed significant sequence
homology to annotated genes with known function. The chromosomal locations of 36 microsatellites were associated with 14 chromosomes
and/or 13 chromosome arms of the cotton genome by hypoaneuploid deficiency analysis, enabling us to assign genetic linkage
groups (LG) to specific chromosomes. The resulting genetic map consists of 193 loci, including 121 new fiber loci not previously
mapped. These fiber loci were mapped to 19 chromosomes and 11 LG spanning 1277 cM, providing approximately 27% genome coverage.
Preliminary quantitative trait loci analysis suggested that chromosomes 2, 3, 15, and 18 may harbor genes for traits related
to fiber quality. These new PCR-based microsatellite markers derived from cotton fiber ESTs will facilitate the development
of a high-resolution integrated genetic map of cotton for structural and functional study of fiber genes and MAS of genes
that enhance fiber quality.
Electronic Supplementary Material Supplementary material is available for this article at
Names are necessary to report factually on available data, however, the USDA neither guarantees nor warrants the standard
of products or service, and the use of the name by the USDA implies no approval of the products or service to the exclusion
of others that may also be suitable. 相似文献
5.
Zhang Z Rong J Waghmare VN Chee PW May OL Wright RJ Gannaway JR Paterson AH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(7):1075-1088
Seventeen backcross-self families from crosses between two Gossypium hirsutum recurrent parent lines (CA3084, CA3093) and G. tomentosum were used to identify quantitative trait loci (QTLs) controlling fiber quality traits. A total of 28 QTLs for fiber quality
traits were identified (P < 0.001), including four for fiber elongation, eight for fiber fineness, four for fiber length, four for fiber strength,
six for fiber uniformity, one for boll weight, and one for boll number. Three statistically significant marker–trait associations
for lint yield were found in a single environment, but need further validation. Two-way analysis of variance revealed one
locus with significant genotype × family interaction (P < 0.001) for fiber strength and a second locus with significant genotype × environment interaction (P < 0.001) in the CA3084 background, and two loci with significant genotype × background interaction (P < 0.001) for the 28 common markers segregating in both of the two recurrent backgrounds. Co-location of many QTLs for fiber
quality traits partially explained correlations among these traits. Some G. tomentosum alleles were associated with multiple favorable effects, offering the possibility of rapid genetic gain by introgression.
Many G. tomentosum alleles were recalcitrant to homozygosity, suggesting that they might be most effectively deployed in hybrid cottons. DNA
markers linked to G. tomentosum QTLs identified in the present study promise to assist breeders in transferring and maintaining valuable traits from this
exotic source during Upland cotton cultivar development. This study also adds further evidence to prior studies indicating
that the majority of genetic variation associated with fiber quality in tetraploid cotton traces to the D-subgenome from a
diploid ancestor that does not produce spinnable fiber. 相似文献
6.
The objectives of this study were to understand the genetic basis of morphological variation observed in the genus Citrus and its relatives and to identify genomic regions associated with certain morphological traits using genetic linkage mapping
and quantitative trait loci (QTLs) analysis with random amplified polymorphic DNA (RAPD) markers. First, a genetic linkage
map was constructed with RAPD markers obtained by screening 98 progeny plants from a {Citrus grandis × [C. paradisi × Poncirus trifoliata]} × {[(C. paradisi × P. trifoliata) × C. reticulata] × [(C. paradisi × Poncirus trifoliata) × C. sinensis]} intergeneric cross. The map contains 69 RAPD markers distributed into nine linkage groups. Then, 17 different morphological
traits, including six tree and two leaf characters of 98 progeny plants and six floral and three fruit characters of about
half of the same progeny plants were evaluated for 2 years and statistically analyzed for variation. Statistical analysis
of individual traits indicated that trunk diameter and growth, tree height, canopy width, tree vigor and growth, leaf length
and width, petal and anther numbers, petal length and width, length of pistil and style, fruit length and diameter, and fruit
segment number showed normal or close to normal distribution, suggesting that these traits may be inherited quantitatively.
Quantitative data from the morphological traits were analyzed to detect markers and putative QTLs associated with these traits
using interval mapping method. QTL analysis revealed 18 putative QTLs of LOD > 3.0 associated with 13 of the morphological
traits analyzed. The putative QTLs were distributed in several different linkage groups, and QTLs associated with similar
traits were mostly mapped to the same LG or similar locations in the linkage group, indicating that the same genomic region
is involved in the inheritance of some of the morphological traits. 相似文献
7.
8.
QTL mapping in A-genome diploid Asiatic cotton and their congruence analysis with AD-genome tetraploid cotton in genus Gossypium 总被引:1,自引:0,他引:1
Xuexia Ma Yezhang Ding Baoliang Zhou Wangzhen Guo Yanhui Lv Xiefei Zhu Tianzhen Zhang 《遗传学报》2008,35(12):751-762
Asiatic cotton(Gossypium arboreum L.) is an Old World cultivated cotton species.The sinense race was planted extensively in China.Due to the advances in spinning technology during the last century,the species was replaced by the New World allotetraploid cotton G.hirsutum L.Gossypium arboreum is still grown in India and Pakistan and also used as an elite in current cotton breeding programs.In addition,G.arboreum serves as a model for genomic research in Gossypium.In the present study,we generated an A-genome diploid cotton intraspecific genetic map including 264 SSR loci with three morphological markers mapped to 1 3 linkage groups.The map spans 2,508.71 cM with an average distance of 9.4 cM between adjacent loci.A population containing 1 76 F2:3 families was used to perform quantitative trait loci(QTL)mapping for 17 phenotypes using Multiple QTL Model(MQM)of MapQTL ver 5.0.Overall,108 QTLs were detected on 13 chromosomes.Thirty-one QTLs for yield and its components were detected in the F2 population.Forty-one QTLs for yield and its components were detected in the F2:3 families with a total of 43 QTLs for fiber qualities.Two QTLs for seed cotton weight/plant and lint index and three QTLs for seed index were consistently detected both in F2 and F2:3.Most QTLs for fiber qualities and yields were located at the same interval or neighboring intervals.These results indicated that the negative correlation between fiber qualities and yield traits may result from either pleiotropic effect of one gene or linkage effects of multiple closely linked genes. 相似文献
9.
Avishag Levi Andrew H. Paterson Vered Barak Dan Yakir Baohua Wang Peng W. Chee Yehoshua Saranga 《Molecular breeding : new strategies in plant improvement》2009,23(2):179-195
Quantitative trait loci (QTLs) for yield and drought related physiological traits, osmotic potential (OP), carbon isotope
ratio (δ13C, an indicator of water use efficiency), and leaf chlorophyll content (Chl), were exchanged via marker-assisted selection
(MAS) between elite cultivars of the two cotton species Gossypium barbadense cv. F-177 and G. hirsutum cv. Siv’on. The resulting near isogenic lines (NILs) were examined in two field trials, each with two irrigation regimes,
in order to (1) evaluate the potential to improve cotton drought resistance by MAS and (2) test the role of physiological
traits in plant productivity. NILs introgressed with QTLs for high yield rarely exhibited an advantage in yield relative to
the recipient parent, whereas a considerable number of NILs exhibited the expected phenotype in terms of lower OP (5 out of
9), higher δ13C (4 out of 6) or high Chl (2 out of 3). Several NILs exhibited considerable modifications in non-targeted traits including
leaf morphology, stomatal conductance and specific leaf weight (SLW). In G. barbadense genotypes, yield was correlated negatively with δ13C and OP and positively with stomatal conductance, SLW and Chl, whereas in G. hirsutum yield was negatively correlated with δ13C, SLW and Chl. This dissimilarity suggests that each of the respective species has evolved different mechanisms underlying
plant productivity. We conclude that the improvement of drought related traits in cotton NILs may lead to improved drought
resistance via MAS, but that conventional breeding may be necessary to combine the introduced QTL(s) with high yield potential. 相似文献
10.
Zheng X Wu JG Lou XY Xu HM Shi CH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,116(3):335-342
Investigations to identify quantitative trait loci (QTLs) governing cooking quality traits including amylose content, gel
consistency and gelatinization temperature (expressed by the alkali spread value) were conducted using a set of 241 RIL populations
derived from an elite hybrid cross of “Zhenshan 97” × “Minghui 63” and their reciprocal backcrosses BC1F1 and BC2F1 populations in two environments. QTLs and QTL × environment interactions were analyzed by using the genetic model with endosperm
and maternal effects and environmental interaction effects on quantitative traits of seed in cereal crops. The results suggested
that a total of seven QTLs were associated with cooking quality of rice, which were subsequently mapped to chromosomes 1,
4 and 6. Six of these QTLs were also found to have environmental interaction effects. 相似文献