极小种群野生植物峨眉含笑的种群结构与数量动态

该研究以极小种群野生植物——峨眉含笑(Michelia wilsonii)为对象,采用样地调查法对雅安市周公山峨眉含笑野生种群进行群落调查,依据空间代替时间理论及匀滑技术,将种群动态变化情况量化,编制种群静态生命表,绘制存活曲线、死亡率曲线、消失率曲线和生存函数曲线分析其年龄结构现状,并用时间序列预测模型对其未来的数量动态进行预测,以揭示峨眉含笑的生存潜力和濒危机制,为该种群的恢复与重建提供依据。结果表明：(1)研究区内峨眉含笑种群的数量变化动态指数V_pi和V_pi′均大于0,年龄结构呈倒J型,存活曲线趋于Deevey Ⅱ型,种群结构属于增长型,但种群的演替过程存在波动性,幼苗的数量丰富但向幼树的发展受阻,幼树向中、壮树阶段的补充也有所减少,种群存在衰退风险。(2)峨眉含笑野生种群的死亡率和消失率均在第Ⅰ龄级、第Ⅶ~Ⅷ龄级和第Ⅹ龄级出现高峰,而在第Ⅱ~Ⅵ龄级阶段比较稳定。(3)随龄级增加,峨眉含笑种群的生存率持续下降,累积死亡率持续上升,危险率在第Ⅰ龄级时最高(1.308),死亡密度在第Ⅶ~Ⅷ龄级降至最低,在整个龄级阶段中对应龄级的危险率均高于生存率,种群存在断代衰退的风险。(4)时间序列预测分析表明,经历未来2、4、6、8个龄级时间后,中、壮树阶段个体数量有减少的趋势。研究认为,峨眉含笑野生种群具有前期锐减、中期稳定、后期衰退的特点。     

长白山北坡天然次生林典型建群种的种群结构及动态特征

分析长白山林区典型天然次生林的建群种种群结构及动态特征,揭示关键种群的生存现状和发展趋势,以期为研究区的天然林保护与修复提供基础资料和理论依据。基于24块1 hm²的固定样地数据,通过编制种群静态生命表,拟合并绘制种群存活曲线,运用生存分析、种群数量化分析和时间序列分析,定量研究2种典型天然次生林4个建群种的种群结构与动态特征。结果显示,4个种群的存活曲线总体均趋于Deevey-Ⅱ型,但所属亚型有所区别。臭冷杉（Abies nephrolepis）种群死亡率波动较大,在不同龄级出现了多次死亡高峰;鱼鳞云杉（Picea jezoensis var. microsperma）和蒙古栎（Quercus mongolica）种群死亡率随龄级增大逐步递增;红松（Pinus koraiensis）种群在各龄级上的死亡率均较高。数量化动态分析表明,4个种群均属于增长型,增长潜力为红松 > 臭冷杉 > 鱼鳞云杉 > 蒙古栎;4个种群受外界干扰的敏感程度均较高,其中红松种群受干扰的概率最大。时间序列预测表明,臭冷杉和红松种群个体数量在未来2、4、6、8个龄级后均呈现不同幅度的增加趋势,增长势态稳定。鱼鳞云杉和蒙古栎种群在幼、中龄级表现出衰退迹象。结论表明,臭冷杉和红松种群的自然更新较好,增长潜力较大,但同时受外界干扰的敏感程度也较高。鱼鳞云杉和蒙古栎种群的自然更新不足,增长潜力小,群落存在偏离稳定状态的风险。建议严格保护臭冷杉和红松种群的生境,适度开展疏伐抚育;改善鱼鳞云杉和蒙古栎种群空间格局,及时实施人工促进天然更新,促进群落进展演替。     

海南铜鼓岭鸭脚木种群动态特征研究

鸭脚木(Schefflera octophylla)是海南文昌铜鼓岭国家级自然保护区内滨海森林的优势种,也是海南其他地区热带森林常见伴生种。为深入了解该区滨海森林内鸭脚木种群的生存现状、更新机制以及未来发展的动态变化特点,该研究通过对海南热带滨海森林2.56 hm²样地中鸭脚木种群的调查,以径级结构代替龄级结构,编制鸭脚木种群静态生命表,并结合种群动态量化指数、生存函数、时间序列预测模型等方法定量分析鸭脚木种群结构和数量动态变化。结果表明:(1)研究区域内共记录鸭脚木数量2 814株,按照径级大小共划分为12个龄级,龄级结构呈倒J字型,属于趋向稳定型种群。(2)该区鸭脚木的存活曲线趋于Deevey-Ⅱ型,种群各径级的死亡率相接近。(3)鸭脚木种群的量化指数显示,V_pi=30.685>0,V_pi''=0.236>0,说明该种群现处增长阶段并且相对稳定。(4)据时间序列模型预测鸭脚木种群在未来的3、6、9 a内各龄级的种群个体数量整体呈现增加的趋势。综上分析认为,该区生境有利于鸭脚木种群的生长且该种群形成了良好的生存策略,其幼龄个体较多且后备资源丰富,能较好地补充各龄级个体自然死亡造成的损失,对森林的天然更新起到一定促进作用     

长白山濒危植物牛皮杜鹃(Rhododendron chrysanthum)种群数量特征与动态分析

牛皮杜鹃为国家Ⅲ级重点保护植物,种群数量有限,处于濒危状态。本文运用种群静态生命表、存活曲线、个体数量动态预测等方法分析了长白山高山苔原带不同海拔牛皮杜鹃种群数量特征及动态发展趋势。结果表明:1)牛皮杜鹃种群大小级结构呈纺锤形,属衰退型种群;2)随海拔升高,种群增长潜力逐渐减小,非完全随机干扰下,种群增长潜力有限,低海拔的Vpi″接近于0;3)种群存活曲线趋于DeeveyⅢ型,高海拔出现较高的死亡率和致死力,种群退化明显;4)在经历1、2、4个龄级后,Ⅳ龄级个体数量会不同程度增加,种群在生长后期个体数量得到补充;5)牛皮杜鹃种群数量主要靠自身匍匐生根形成分株来维持。整个种群向大龄级个体集中,高海拔面临较强的环境胁迫,会比中低海拔更早进入衰退期。导致其濒危的可能原因是牛皮杜鹃的生物学特性及人为干扰。     

极小种群野生植物细果秤锤树种群结构与动态特征

细果秤锤树(Sinojackia microcarpa)是中国特有极小种群植物之一,属国家Ⅱ级重点保护植物。为揭示该种群的生存潜力与濒危机制,为种群保护提供依据,采用样地调查法在浙江省建德市细果秤锤树自然集中分布区进行野外调查,以空间代替时间研究了细果秤锤树种群的结构特征,绘制了种群静态生命表及种群存活曲线,并应用种群动态和时间序列量化预测种群未来发展态势。结果表明:(1)细果秤锤树的径级、高度和冠幅结构均表明该种群龄级结构总体呈衰退型,种群幼年树淘汰率较高,中年树到老年树过渡阶段适应力增强,生长情况较前期稳定。(2)生活曲线属Deevey-Ⅲ型,早期死亡率高,生存分析揭示细果秤锤树种群具有前期锐减的动态特征。(3)时间序列分析预测在未来2、4、6、8个龄级后,细果秤锤树的年龄结构存在幼年个体数下降,中、老年树占优势的衰退风险。(4)种群动态指数表明该种群结构总体波动大,抗外界干扰能力较弱,环境对个体的选择作用较强。为促进种群自然更新,应加强就地保护措施,降低人为干扰对个体生存环境的威胁,提高幼苗至幼树转化率,实现种群恢复。     

黄山常绿阔叶林优势种甜槠的种群动态与点格局分析

该研究以黄山森林植物多样性监测的3个大样地(钓桥庵、云谷寺、望瀑楼)内的优势种——甜槠(Castanopsiseyrei)种群为对象,分别从年龄结构、静态生命表、存活曲线、种群增长模型、生存分析和点格局分析等方面对甜槠种群的数量动态与空间分布格局进行了研究。结果表明：(1)研究区3个样地的甜槠均呈现种群幼苗众多,幼树储备丰富,且各样地个体数均随着龄级的增加逐渐减少,说明研究区甜槠种群均处于增长阶段,更新能力强,属于稳定型种群。(2)钓桥庵和望瀑楼样地的甜槠种群存活曲线均趋近于DeeveyⅡ型,两样地内甜槠各龄级均具有相近的死亡率,出现的3个死亡高峰分别在幼年期、近成熟前期和老龄期。(3)云谷寺样地的甜槠种群存活曲线趋近于DeeveyⅢ型,其前期死亡率较高,中期和后期死亡率比较低而且稳定。(4) 黄山森林植物中甜槠种群在小尺度呈显著的聚集性,随着尺度的增加则表现为随机分布。     

濒危植物澜沧黄杉种群结构及动态特征

澜沧黄杉(Pseudotsuga forrestiiCraib)为中国特有珍稀濒危植物,野外种群个体数量少,分布范围狭窄,研究其种群结构及动态特征可以量化种群未来发展趋势,并为科学合理保护该物种提供依据。通过对云南省迪庆州德钦县不同群落类型下澜沧黄杉种群的调查及数据收集,编制澜沧黄杉静态生命表,绘制存活曲线和生存分析函数曲线,并采用时间序列模型对种群的数量动态进行预测,以揭示不同群落环境下澜沧黄杉的种群结构和动态特征。结果表明:德钦县澜沧黄杉种群主要分布在海拔2200-3300m的区域,栖息地群落类型为澜沧黄杉林、冷杉林与云南松林,三种群落澜沧黄杉种群均属增长型,数量变化动态指数V_pi和V_pi''都大于0,但对外界干扰比较敏感,存活曲线趋于Deevey-Ⅱ型,表明种群各龄级的死亡率基本接近。不同群落类型下种群大小依次为澜沧黄杉林 ＞ 冷杉林 ＞ 云南松林,结合种群动态分析表明,澜沧黄杉林更适宜种群的生存;生存分析和时间序列预测表明幼龄个体的缺乏、死亡率高,是导致种群野外更新困难的主要原因。野外调查发现澜沧黄杉天然种群受到人类砍伐、放牧、开荒等因素的影响,严重影响了种群的生存,因此未来在加大科学研究力度进一步探究该物种濒危影响因素的同时,还要加大保护宣传力度,并扩大建立保护区以实现对该物种的多方位保护。     

甘南高山林线岷江冷杉—杜鹃种群结构与动态

高山林线是一种典型的生态交错带,是对气候反映最敏感的地区之一。甘肃南部高山林线区域主要以原始岷江冷杉种群和杜鹃种群为优势种,通过对岷江冷杉和杜鹃种群建立静态生命表,绘制存活曲线描述其结构特征,利用种群数量动态预测时间序列分析定量研究未来的发展趋势。结果显示:(1)岷江冷杉种群幼苗比较丰富,能很好的维持种群个体的自疏死亡,存活曲线呈Deevey-Ⅲ型;杜鹃种群幼苗缺乏,存活曲线趋向于Deevey-Ⅰ型;死亡曲线和危险率曲线都随着龄级的增加而增加,杜鹃种群的死亡率在各个龄级一直大于岷江冷杉种群,危险率在Ⅱ龄级以后杜鹃种群也始终大于岷江冷杉种群。(2)岷江冷杉种群结构动态变化指数Vpi大于修正后的种群结构动态变化指数V′pi且大于0,而杜鹃种群结构动态变化指数Vpi小于修正后的种群结构动态变化指数V′pi且小于0,则岷江冷杉种群属于增长型,杜鹃种群属于衰退型,岷江冷杉、杜鹃随机干扰极大值分别为0.027、0.011,说明二者对外界干扰均比较敏感。(3)杜鹃种群时间序列预测为前期幼苗比较缺乏,中期稳定,后期衰退的动态特征,而岷江冷杉种群表现出各龄级时间变化较小,幼苗个体数较多,种群为稳定增长型,岷江冷杉更能适应甘肃南部高山林线区域当前环境。     

准噶尔盆地东南缘梭梭种群结构与动态研究

通过样地调查和数据统计分析,对准噶尔盆地东南缘梭梭种群现状绘制径级结构图,编制生命表、分析存活曲线、死亡率曲线和寿命期望等参数,并运用时间序列模型预测种群数量动态.结果表明:种群整体结构属于增长型,但梭梭种群幼苗个体少,Ⅰ、Ⅱ龄级个体数目少于Ⅲ龄级的个体数目.存活曲线基本接近DeeveyⅢ型,种群偏离典型存活曲线的程度与幼苗缺乏有关;环境因素和人为干扰的影响,梭梭在Ⅲ～V龄级死亡率较高,种群在第Ⅳ级出现死亡高峰,且只有少量幼年个体能进入成年阶段,个体平均生存能力的期望在第Ⅳ级最大;时间序列分析表明,在未来第Ⅱ、Ⅳ、Ⅵ、Ⅷ龄级的个体会呈现老龄个体增加的趋势,种群稳定性长期维持困难.适当的人工辅助恢复和现有植株及生境的保护是保持梭梭种群自然更新和种群恢复的关键.     

基于生命表的大兴安岭兴安杜鹃(Rhododendron dauricum)种群数量特征及动态分析

兴安杜鹃(Rhododendron dauricum)是大兴安岭森林群落灌木层的优势种,其种群统计研究尚属空白。以大兴安岭呼中区不同海拔和林型的兴安杜鹃种群为对象,编制了静态生命表,计算生存分析函数,采用时间序列分析种群数量动态。结果表明,低海拔梯度(600m)的落叶松林中,兴安杜鹃幼龄级(Ⅰ-Ⅱ)个体较多,占67.31%,且幼龄级死亡率和消失率较低,存活率较高,存活曲线为Deevey Ⅰ型曲线,为增长型种群。种群的衰退期转折点在Ⅱ-Ⅲ龄级,未来种群数量呈增长趋势。中低海拔梯度(600-900m)的白桦林中,中龄级(Ⅱ-Ⅲ)个体数量较多,占45%以上,中龄级死亡率和消失率较低,存活率较高,存活曲线为Deevey Ⅰ型曲线,为稳定型种群。种群的衰退期转折点在Ⅲ-Ⅴ龄级之间,由于种群密度制约,未来种群数量逐渐趋于衰退。高海拔梯度(1200m)的落叶松林中,种群个体总数最少。其中,Ⅰ龄级个体数量最多,占33.52%,但死亡率和消失率较高,存活率较低,Ⅱ-Ⅴ龄级死亡率和消失率较低,存活率较高,存活曲线为Deevey Ⅲ型曲线,为增长型种群,但增长趋势较弱。种群的衰退期转折点在Ⅴ龄级,未来种群数量呈增长趋势,但由于环境限制,种群数量更趋于稳定。可以认为,大兴安岭兴安杜鹃种群数量整体稳定。海拔和林型是影响兴安杜鹃种群数量的重要因素,海拔为600-900m,郁闭度为45%-50%的白桦林为兴安杜鹃的适宜生境,成为灌木层中的主要优势种。     

The pollination ecology of Pedicularis rex subsp. lipkyana and P. rex subsp. rex (Orobanchaceae) from Sichuan, southwestern China

Pedicularis is one of few genera for which pollination ecology has been studied extensively. Although over half of the species of Pedicularis are found in the mountains of southwestern China, pollination ecology has been studied there on a few species only. The present paper reports pollination ecology of Pedicularis rex subsp. lipskyana and P. rex subsp. rex from Sichuan, southwestern China. The two subspecies are nectariferous, with sugar content 28% in P. rex lipskyana and 22% in P. rex, respectively. The flowers of the two subspecies are adapted to pollination by bumblebee workers. Bumblebees are the primary and effective pollinators although honeybees were also observed pollinating P. rex subsp. lipskyana at one studied population. Usually bumblebees entered the corolla tube from the right side in an upright or a nearly half-inverted position to imbibe nectar located at the base of the corolla tube. The stigma usually contacts the side region of the thorax and abdomen or occasionally directly contacted pollen loads, leading to pollination of flowers. Much less commonly, bumblebees foraged on flowers of P. rex subsp. rex in an inverted position for pollen, which pollinated flowers sternotribically. We suggest that pollination mechanism is closely associated with floral morphology. Some important differences were found from a previous study on P. rex in floral morphology and the primary pollination mechanism.     

云南10种杜鹃属植物的叶片覆被物特征及其系统学意义

采用扫描电子显微镜对云南10种杜鹃属植物叶片毛被与鳞片特征进行了研究,并结合已报道的中国45种杜鹃属植物毛被与鳞片特征进行了聚类分析,探讨叶表皮毛被与鳞片在该属植物中的系统学意义。结果表明:(1)常绿杜鹃亚属的8种植物叶背面具毛被却无鳞片结构,杜鹃花亚属的2种植物叶背面均具鳞片结构。(2)基于叶表面毛被与鳞片特征对55种杜鹃属植物进行聚类分析显示,有鳞片杜鹃与无鳞片杜鹃分别聚为2类,聚类结果与杜鹃属植物在亚属水平上的传统分类基本一致,表明叶表面毛被与鳞片特征在杜鹃属植物各亚属的分类中具有较好的参考价值。(3)从亚组水平看,55种杜鹃属植物的聚类结果对云锦杜鹃亚组、有鳞大花亚组、露珠杜鹃亚组具有较好的分类学界定,但在三花杜鹃亚组、高山杜鹃亚组、大理杜鹃亚组、大叶杜鹃亚组、树形杜鹃亚组存在明显的交叉现象,表明叶表皮覆被物特征不足以作为这些亚组在分类处理上的关键性状。(4)该研究聚类结果表明,糙叶杜鹃与其他有鳞杜鹃之间亲缘关系较近,支持糙叶杜鹃归并到杜鹃花亚属。     

Presence of Rhizobium Etli bv . Phaseoli and Rhizobium Gallicum bv . Gallicum in Egyptian Soils

Seven bean rhizobial strains EBRI 2, 3, 21, 24, 26, 27 and 29 identified as Rhizobium etli, and EBRI 32 identified as Rhizobium gallicum, isolated from Egyptian soils and which nodulated Phaseolus vulgaris efficiently, were subjected to hybridization with a nifH probe in order to estimate the copy number of this gene. Seven strains (EBRI 2, 3, 21, 24, 26, 27 and 29) which were only able to nodulate Phaseolus vulgaris, contained three copies of the nifH gene, consistent with their identification as Rhizobium etli bv. phaseoli. Only one strain (EBRI 32) which nodulated both Phaseolus vulgaris and Leucaena leucocephala, had one copy of nifH gene. This confirmed the classification of this strain as Rhizobium gallicum bv. gallicum.     

Effects of MULTIFOLIATE-PINNA, AFILA, TENDRIL-LESS and UNIFOLIATA genes on leafblade architecture in Pisum sativum

In order to dissect the genetic regulation of leafblade morphogenesis, 16 genotypes of pea, constructed by combining the wild-type and mutant alleles of MFP, AF, TL and UNI genes, were quantitatively phenotyped. The morphological features of the three domains of leafblades of four genotypes, unknown earlier, were described. All the genotypes were found to differ in leafblade morphology. It was evident that MFP and TL functions acted as repressor of pinna ramification, in the distal domain. These functions, with and without interaction with UNI, also repressed the ramification of proximal pinnae in the absence of AF function. The expression of MFP and TL required UNI function. AF function was found to control leafblade architecture multifariously. The earlier identified role of AF as a repressor of UNI in the proximal domain was confirmed. Negative control of AF on the UNI-dependent pinna ramification in the distal domain was revealed. It was found that AF establishes a boundary between proximal and distal domains and activates formation of leaflet pinnae in the proximal domain.     

Characterisation of cell wall polysaccharides, arabinogalactans-proteins (AGPs) and phenolics of Cola nitida, Cola acuminata and Garcinia kola seeds

Many Cola plant species are endemic to West and Central Africa. Cola acuminata and Cola nitida are used as masticatory when fresh, while the dried nuts are used for beverages and pharmaceutical purposes in Europe and North America. Garcinia kola seeds, that serve as a substitute for the true kola nuts, are used in African traditional medicine for the treatment of various diseases, including colic, headache and liver cirrhosis. Seeds extracts of G. kola are also known for their anti-inflammatory, antimicrobial and antiviral properties. To gain information on the chemical properties of the kolas, we have isolated and analyzed cell wall polysaccharides, arabinogalactan-proteins and phenolic substances from the seeds of the three kola species. The sugar composition of cell wall material of C. acuminata, C. nitida and G. kola revealed that Gal (up to 30%), Ara, GalA and Glc as the predominant monosaccharides, representing approximately 90% by mol of the total hydrolysable sugar present in this material. In Ammonium oxalate cell wall fraction, GalA was found to be the major sugar present in all kola species. In the alkali-soluble fraction, there were significant differences in the level of Glc and Gal. The level of Glc was high in C. acuminata and C. nitida while the level of Gal and Xyl were high in C. nitida and G. cola. Isolation and quantification of arabinogalactan-proteins demonstrate that G. kola seeds contained four to eight times more of these proteoglycans than the seeds of the other two species. Finally, analysis of soluble phenolic substances shows that caffeine and catechin were largely represented in C. acumina and C. nitida seeds, with caffeine accounting for 50% of all soluble phenolics. These findings indicate that the three Kola seeds are highly enriched in pectins and proteoglycans and that C. acuminata and C. nitida can be used as a possible source of caffeine and catechin.     

Evolutionary relationships among aquatic anamorphs and teleomorphs: Lemonniera, Margaritispora, and Goniopila

The hypothesis that similar conidial morphologies in aquatic hyphomycetes are a result of convergent evolution was tested using molecular sequence data. Cladistic analyses were performed on partial sequences of 28S rDNA of seven species of Lemonniera, one species of Margaritispora and one species of Goniopila. Lemonniera has tetraradiate conidia with long arms, whereas Margaritispora and Goniopila have typically globose (isodiametric) conidia, with short conical protuberances in a stellate or quadrangular arrangement. Lemonniera and Margaritispora have phialidic conidiogenesis and both produce dark, minute sclerotia in culture whereas Goniopila has holoblastic conidiogenesis and does not produce sclerotia in culture. Goniopila produces a microconidial phialidic synanamorph in culture. All three genera have schizolytic conidial secession. Molecular analyses demonstrate that Lemonniera species are placed in two distinct clades: one within Leotiomycetes; the other within Pleosporales, Dothideomycetes. Margaritispora is placed with Lemonniera species within Leotiomycetes. Goniopila and Lemonniera pseudofloscula are placed within Dothideomycetes. No morphological character was entirely congruent with the molecular derived phylogeny. This suggests that for the group of species studied, conidial shape is not a reliable indicator of phylogeny but more likely the result of convergent evolution in response to the aquatic environment.     

Hydroxylation of steroids by Fusarium oxysporum, Exophiala jeanselmei and Ceratocystis paradoxa

The potential of Fusarium oxysporum var. cubense UAMH 9013 to perform steroid biotransformations was reinvestigated using single phase and pulse feed conditions. The following natural steroids served as substrates: dehydroepiandrosterone (1), pregnenolone (2), testosterone (3), progesterone (4), cortisone (5), prednisone (6), estrone (7) and sarsasapogenin (8). The results showed the possible presence of C-7 and C-15 hydroxylase enzymes. This hypothesis was explored using three synthetic androstanes: androstane-3,17-dione (9), androsta-4,6-diene-3,17-dione (10) and 3α,5α-cycloandrost-6-en-17-one (11). These fermentations of non-natural steroids showed that C-7 hydroxylation was as a result of that position being allylic. The evidence also pointed towards the presence of a C-15 hydroxylase enzyme.The eleven steroids were also fed to Exophialajeanselmei var. lecanii-corni UAMH 8783. The results showed that the fungus appears to have very active 5α and 14α-hydroxylase enzymes, and is also capable of carrying out allylic oxidations.Ceratocystis paradoxa UAMH 8784 was grown in the presence of the above-mentioned steroids. The results showed that monooxygenases which effect allylic hydroxylation and Baeyer–Villiger rearrangement were active. However, redox reactions predominated.     

Genome Analysis inNeurospora crassa;Cloning of Four Lociarginine-1(arg-1),methionine-6(met-6),unknown-7(un-7), andribosome production-1(rip-1) and Associated Chromosome Walking

We have cloned fourNeurospora crassagenes by complementation analysis. Cloned genes include thearginine-1(arg-1),methionine-6(met-6),unknown-7(un-7), andribosome production-1(rip-1) loci. Chromosome walks were initiated in ordered cosmid libraries from the cloned loci. A total of about 700 kb of theNeurosporagenome is covered in these walks.     

甘菊ClHSP70与ClHSP90基因的克隆及表达分析

该研究以甘菊(Chrysanthemum lavandulifolium)为实验材料,通过RT-PCR方法从甘菊转录组数据中分离出热激蛋白合成相关基因,命名为ClHSP70和ClHSP90。序列分析表明,ClHSP70基因ORF全长为2 559bp,编码852个氨基酸,蛋白功能区预测表明含有典型的HSP70蛋白NBD和SBD保守结构域;ClHSP90基因ORF全长为2 094bp,编码697个氨基酸,含有HATPase结构域和HSP90保守结构域。生物信息学分析表明,甘菊ClHSP70与大豆(Glycine max)和烟草(Nicotiana tomentosiformis)HSP70蛋白有较高的一致性,ClHSP90基因编码的氨基酸序列与紫茎泽兰(Ageratina adenophora)HSP90高度相似;实时荧光定量表达分析表明,在42℃处理不同时间,甘菊叶片中ClHSP70和ClHSP90基因表达均在0.5h时显著增加,1h达到最大值,2h后缓慢下降;不同组织表达分析表明,甘菊在42℃处理1h后,ClHSP70在成熟叶中的表达量显著高于嫩叶和根等其他组织;ClHSP90在成熟茎中的表达量最高。研究说明,ClHSP70和ClHSP90基因具有热激蛋白特征,参与了甘菊热胁迫应答过程,该研究结果为以后深入研究其基因功能奠定了基础。