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1.
M. E. WESTERMAN V. P. BUONACCORSI J. A. STANNARD L. GALVER C. TAYLOR E. A. LYNN C. A. KIMBRELL R. D. VETTER 《Molecular ecology resources》2005,5(1):74-76
Here we report development and characterization of seven polymorphic loci derived from grass rockfish (Sebastes rastrelliger) genomic DNA phagemid libraries enriched for microsatellite motifs. Within grass rockfish, average allelic diversity was 11.3 alleles per locus and average heterozygosity was 0.73. The seven loci also were surveyed in 10 related species of Sebastes, where allelic diversity ranged from highly polymorphic to monomorphic. Deviations from Hardy–Weinberg were nonsignificant in all but one species/locus combination suggesting low occurrence of null alleles. Significant linkage disequilibrium was detected among three loci, but these events were limited to a single species in each case. 相似文献
2.
TINA KYNDT ANNELIES HAEGEMAN SABINE VAN GLABEKE INGE MAERTENS BART VAN DROOGENBROECK ISABEL ROLDN‐RUIZ GODELIEVE GHEYSEN 《Molecular ecology resources》2005,5(3):590-592
Nine polymorphic microsatellite loci were identified in the tropical plant Vasconcellea ×heilbornii and used to estimate allelic diversity in two populations of southern Ecuador. Allelic richness ranged from two to five alleles, observed heterozygosity ranged from 0.150 to 0.947 and expected heterozygosity ranged from 0.186 to 0.701. Most of these markers also amplified microsatellite loci in two other Vasconcellea species (Vasconcellea stipulata and Vasconcellea cundinamarcensis). Hence, these markers will be useful for population genetic analysis and the evaluation of genetic diversity and gene flow in these species. 相似文献
3.
Weitao Li Xiaolin Liao Nian Xu Yanfu Que Meihua Xiong Bin Zhu Ke Shao 《Zeitschrift fur angewandte Ichthyologie》2019,35(4):1025-1028
Homatula potanini is a genus Paracobitis which belongs to the family Cobitidae, subfamily Nemacheilinae. We first isolated ten novel microsatellite DNA loci using the FIASCO protocol. The number of observed alleles per locus ranged from 5 to 14. The observed heterozygosity and expected heterozygosity ranged from 0.3750 to 0.9375 and 0.4028 to 0.8819 respectively. The polymorphic information content ranged from 0.3515 to 0.8556 (M = 0.6170). No significant linkage disequilibrium was detected among the loci. In the present study, we have identified 10 new markers for H. potanini. These loci should provide sufficient levels in the evaluation of genetic diversity and designing effective conservation programs of H. potanini. 相似文献
4.
Shichao Xing Changwei Shao Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(3):789-791
In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library
of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909
to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result,
13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide
sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy
in Nibea albiflora.
Shichao Xing, Changwei Shao contributed equally to this work. 相似文献
5.
Shi-Chao Xing Gen-Bo Xu Xiao-Lin Liao Guan-Pin Yang Song-Lin Chen 《Conservation Genetics》2009,10(4):1167-1169
In the study, 34 microsatellite loci were isolated from a dinucleotide-enriched genomic library of Japanese Spanish mackerel
(Scomberomorus niphonius). And 12 microsatellite loci were found to be polymorphic between 3 and 8 alleles. The number of observed and expected heterozygosity
per locus in 23 individuals ranged from 0.6087 to 1.0000 and 0.8908 to 0.9773, respectively. Two loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found
between pairs of loci. As a result, 12 microsatellite loci probably located on different chromosome pairs and these polymorphic
microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure
and evaluate the breeding strategy in S. niphonius.
The authors Shi-Chao Xing and Gen-Bo Xu contributed equally. 相似文献
6.
Zhen Shen Bo Wang Fawen Hu Hongzhan Liu Hongbin Guan Fengrong Zheng 《Zeitschrift fur angewandte Ichthyologie》2020,36(1):55-61
In this study, we isolated 20 novel microsatellites loci associated with the growth of Hexagrammos otakii (Fat greenling) by using 2b-RAD sequencing method. The characteristics of 20 microsatellite loci were amplified in 105 H. otakii individuals which came from four different groups and tested by Capillary electrophoresis. The number of allele of the 20 microsatellite loci ranged from 8 to 26 with an average of 19.95. PIC value ranged from 0.1659 to 0.9227 with an average of 0.7555. Observed and expected heterozygosity varied from 0.0667 to 0.8571 and from 0.1710 to 0.9314, respectively. Highly polymorphic characteristics were observed in each microsatellite loci of the study except HO6. Further research showed that 10 microsatellite loci had transferability in Hexagrammos agrammus. In this study, high polymorphism and genetic diversity using the 15 polymorphic microsatellite loci suggest that they are suitable for investigating the fine-scale population structure, genetic relationship and further evaluating the artificial reproduction strategy of H. otakii. 相似文献
7.
Hong-Li Tian Xiao-Qing Chen Jian-Xiu Wang Jian-Hua Xue Jun Wen Grant Mitchell Shi-Liang Zhou 《Conservation Genetics》2008,9(5):1385-1388
This paper reports the development of microsatellite primers for Nelumbo nucifera Gaerten. By screening genomic libraries enriched with 10 kinds of probes, Seventeen polymorphic loci were isolated and primers
were designed. Polymorphism of these 17 loci was assessed in 24 individuals. All the 17 loci are polymorphic and the number
of alleles ranged from two to seven. Observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.9176 and
from 0.2837 to 0.7917 respectively. These microsatellite loci should be useful for studying the genetic diversity of N. nucifera. 相似文献
8.
JÜRG ENKERLI ROLAND K
LLIKER SIEGFRIED KELLER FRANCO WIDMER 《Molecular ecology resources》2005,5(2):384-386
Fourteen polymorphic microsatellite markers were isolated from the entomopathogenic fungus, Metarhizium anisopliae, based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 34 isolates sampled from across Switzerland. The number of detected alleles in 14 loci ranged from two to eight and expected heterozygosity from 0.265 to 0.808. Because of the high expected heterozygosity, the 14 microsatellite loci are very useful for ecological studies and analysis of population diversity, and to identifying, monitoring, and tracking M. anisopliae strains applied as biological control agents. 相似文献
9.
The ark shell Scapharca subcrenata is an important fishery resource, but has been suffering from severe population decline due to the deterioration of the coastal
environment and over-exploitation. To investigate the genetic variation and structure among populations of S. subcrenata, 14 polymorphic microsatellite loci were developed. The number of alleles per polymorphic locus ranged from 11 to 29 and
the observed and expected heterozygosity varied from 0.400 to 0.923 and from 0.705 to 0.965, respectively. These microsatellites
will help advance the investigation of the genetic population structure and genetic diversity in this species. 相似文献
10.
Shui Ming Zhang Chang Jie Xu Zhong Shan Gao Kun Song Chen Guo Yun Wang 《Conservation Genetics》2009,10(5):1605-1607
Chinese bayberry (Myrica rubra Sieb. & Zucc.) is one of the most important fruit tree crops in southern China. This paper reports the development of microsatellite
primers for Chinese bayberry. By screening the expressed sequence tag database and other sources, 11 polymorphic loci were
generated and primers were designed. Polymorphism of these 11 loci was assessed in 32 cultivars. All the 11 loci are polymorphic
and the number of alleles (A) ranged from 3 to 12, observed heterozygosity (H
O) and expected heterozygosity (H
E) ranged from 0.1250 to 0.9667 and 0.2359 to 0.8790, respectively, polymorphism information content ranged from 0.2285 to
0.8516. These microsatellite loci should be useful in the studies of genetic diversity of Myrica rubra and will provide useful implications for resource conservation. 相似文献
11.
The present study reports isolation and characterization of 11 polymorphic microsatellite markers for Nycticorax nycticorax. A total of 82 alleles were detected with an average of 7.5 alleles per locus. The observed heterozygosity (H
O) ranged from 0.25 to 1.00, and the expected heterozygosity (H
E) ranged from 0.51 to 0.88. Analyses revealed no evidence for Linkage disequilibrium between any two loci, and only one locus
was significantly deviated from HWE with the estimation of exact P values by the Markov chain method (P < 0.001). The 11 loci were successfully amplified in 11 other Ardeidae species. These results demonstrate these markers can
be used for the study of intra- and inter-specific genetic diversity. 相似文献
12.
In this study we characterized 10 polymorphic microsatellite markers for the land snail Cylindrus obtusus, an endemism of the Austrian Alps with a distribution in isolated populations above approximately 1,600 m. The microsatellite
loci were analyzed in 44 individuals from two populations. Number of alleles per locus ranged between two and eight. Observed
heterozygosity ranged between 0.00 and 1.00, and expected heterozygosity between 0.09 and 0.72. No significant linkage disequilibrium
was found between pairs of loci. One of the sampled populations (Dachstein) showed no deviation from Hardy–Weinberg equilibrium
and no presence of null alleles, whereas the other one (Schneeberg) did. These diverging results probably reflect differences
in population structure rather than characteristics of the microsatellite loci and underline the usefulness of these markers
for studying genetic diversity, population structure and differentiation in C. obtusus. 相似文献
13.
Mary M. Peacock Veronica S. Kirchoff Susan J. Merideth 《Molecular ecology resources》2002,2(3):360-362
Nine polymorphic microsatellite loci were developed for the North American pika (Ochotona princeps) from di‐ and tetranucleotide repeat‐enriched genomic libraries. Polymorphism was assessed for 165 individuals from eight geographical locations in the western United States. All loci were polymorphic. The number of alleles per locus ranged from three to 14, with observed heterozygosity between 0.189 and 0.822. All loci were in Hardy–Weinberg equilibrium (< 0.05). Regional differences were evident with unique alleles at multiple loci in six of eight populations. 相似文献
14.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
15.
A total of 45 microsatellite loci from yellow perch, Perca flavescens, were isolated and characterized. Among the 45 microsatellite loci, 32 had more than two alleles. A wild population of P. flavescens (n = 48) was used to examine the allele range of the microsatellite loci. Mendelian inheritance of alleles was confirmed by examining the amplified products in pair‐mated families. The number of alleles for the 32 polymorphic loci varied from two to 16, and observed heterozygosity ranged between 0.024 (YP79) and 0.979 (YP60). Cross‐species polymorphic amplification in four other Percidae species was successful for 22 loci. 相似文献
16.
Eleven polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Akebia trifoliate ssp. australis. The number of allele per locus ranged from 3 to 14. The observed heterozygosity and expected heterozygosity at population
level were 0.196–1.000 and 0.522–0.902, respectively. In addition, this set of microsatellites produced robust cross-species
amplification in other two related taxa, suggesting these microsatellite markers should provide a useful tool for genetic
and conservation studies of the Akebia species. 相似文献
17.
Marta Vila Neus Marí-Mena Shen-Horn Yen Carlos Lopez-Vaamonde 《Conservation Genetics》2010,11(3):1151-1154
Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals
from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from
5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was
0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium
at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from
this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this
highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna. 相似文献
18.
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged
from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested
for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified. 相似文献
19.
We report the development of 15 new microsatellite markers for Emys orbicularis and Emys trinacris. A survey of 20 individuals showed that all loci are highly polymorphic with 3–14 alleles per locus. Additionally, 22 Glyptemys muhlenbergii primers were checked for cross-species amplification, with 14 being amplified successfully and polymorphic (2–14 alleles).
A set of eight markers was selected and combined into two multiplex PCRs. Levels of genetic diversity were assessed in 648
individuals covering the complete distribution area. The number of alleles ranged from 13 to 24 and observed heterozygosity
varied between 0.515 and 0.852. 相似文献
20.
Yong-Sheng Tian Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(4):1041-1043
Stone flounder (Kareius bicoloratus) is a rare fish species in China. Here, we reported 11 polymorphic microsatellite loci isolated from a repeat-enriched genomic
library of stone flounder. The number of alleles, observed and expected heterozygosity per locus in 32 individuals ranged
from 3 to 6, from 0.1613 to 0.8667 and from 0.1549 to 0.7932, respectively. Two loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species
amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite
loci would be useful for investigating genetic population structure and construction of genetic linkage map in Kareius bicoloratus.
Yong-Sheng Tian and Gui-Dong Miao are contributed equally. 相似文献