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1.
Background
The wild rodent Calomys callosus is notably resistant to Trypanosoma cruzi infection. In order to better characterize this animal model for experimental infections, we inoculated C. callosus intraperitoneally with Paracoccidioides brasiliensis, a thermally dimorphic fungus that causes a chronic disease with severe granuloma formation in the mouse and humans. The dissemination of P. brasiliensis cells through the lungs, liver, pancreas, and spleen was assessed by histological analysis. 相似文献2.
Benedito Rodrigues da Silva Neto Julhiany de Fátima da Silva Maria José Soares Mendes-Giannini Henrique Leonel Lenzi Célia Maria de Almeida Soares Maristela Pereira 《BMC microbiology》2009,9(1):272
Background
The pathogenic fungus Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM). This is a pulmonary mycosis acquired by inhalation of fungal airborne propagules that can disseminate to several organs and tissues leading to a severe form of the disease. Adhesion and invasion to host cells are essential steps involved in the internalization and dissemination of pathogens. Inside the host, P. brasiliensis may use the glyoxylate cycle for intracellular survival. 相似文献3.
Juliana A Parente Sílvia M Salem-Izacc Jaime M Santana Maristela Pereira Clayton L Borges Alexandre M Bailão Célia MA Soares 《BMC microbiology》2010,10(1):292
Background
Paracoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi. 相似文献4.
Guilherme Augusto Biondo Luciane Alarcão Dias-Melicio Ana Paula Bordon-Graciani Michele Janegitz Acorci-Valério Angela Maria Victoriano Campos Soares 《Mycopathologia》2010,170(2):123-130
Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis, the most prevalent deep mycosis in Latin America. Production of eicosanoids during
fungal infections plays a critical role on fungal biology as well as on host immune response modulation. The purpose of our
study was to assess whether P. brasiliensis strains with different degree of virulence (Pb18, Pb265, Bt79, Pb192) produce prostaglandin Ex (PGEx). Moreover, we asked if P. brasiliensis could use exogenous sources of arachidonic acid (AA), as well as metabolic pathways dependent on cyclooxygenase (COX) enzyme,
as reported for mammalian cells. A possible association between this prostanoid and fungus viability was also assessed. Our
results showed that all strains, independently of their virulence, produce high PGEx levels on 4 h culture that were reduced after 8 h. However, in both culture times, higher prostanoid levels were detected
after supplementation of medium with exogenous AA. Treatment with indomethacin, a COX inhibitor, induced a reduction on PGEx,
as well as in fungus viability. The data provide evidence that P. brasiliensis produces prostaglandin-like molecules by metabolizing either endogenous or exogenous AA. Moreover, the results suggest the
involvement of these mediators on fungal viability. 相似文献
5.
6.
André M Nicola Rosângela V Andrade Alessandra S Dantas Patrícia A Andrade Fabrício BM Arraes Larissa Fernandes Ildinete Silva-Pereira Maria Sueli S Felipe 《BMC microbiology》2008,8(1):158
Background
Paracoccidioides brasiliensis is a dimorphic fungus that causes the most prevalent systemic mycosis in Latin America. The response to heat shock is involved in pathogenesis, as this pathogen switches from mycelium to yeast forms in a temperature dependent fashion that is essential to establish infection. HSP90 is a molecular chaperone that helps in the folding and stabilization of selected polypeptides. HSP90 family members have been shown to present important roles in fungi, especially in the pathogenic species, as an immunodominant antigen and also as a potential antifungal therapeutic target. 相似文献7.
Background
To provide a broad analysis of gene expression changes in developing embryos from a solanaceous species, we produced amplicon-derived microarrays with 7741 ESTs isolated from Solanum chacoense ovules bearing embryos from all developmental stages. Our aims were to: 1) identify genes expressed in a tissue-specific and temporal-specific manner; 2) define clusters of genes showing similar patterns of spatial and temporal expression; and 3) identify stage-specific or transition-specific candidate genes for further functional genomic analyses. 相似文献8.
Mickael?Lafond Alexandra?Tauzin Véronique?Desseaux Estelle?Bonnin El-Hassan?Ajandouz Thierry?Giardina
Background
The filamentous fungus Penicillium funiculosum produces a range of glycoside hydrolases (GH). The XynD gene, encoding the sole P. funiculosum GH10 xylanase described so far, was cloned into the pPICZαA vector and expressed in methylotrophe yeast Pichia pastoris, in order to compare the results obtained with the P. funiculosum GH11 xylanases data. 相似文献9.
Background
Paracoccidioides brasiliensis is a thermo-dimorphic fungus that causes paracoccidiodomycosis (PCM). Glycoprotein gp43 is the fungal main diagnostic antigen, which can also protect against murine PCM and interact with extracellular matrix proteins. It is structurally related to glucanases, however not active, and whose expression varies considerably. We have presently studied polymorphisms in the PbGP43 flanking regions to help understand such variations. 相似文献10.
Julhiany de Fatima da Silva Haroldo César de Oliveira Caroline Maria Marcos Rosangela Aparecida Moraes da Silva Tania Alves da Costa Vera Lucia García Calich Ana Marisa Fusco Almeida Maria José Soares Mendes-Giannini 《PloS one》2013,8(4)
Paracoccidoides brasiliensis adhesion to lung epithelial cells is considered an essential event for the establishment of infection and different proteins participate in this process. One of these proteins is a 30 kDa adhesin, pI 4.9 that was described as a laminin ligand in previous studies, and it was more highly expressed in more virulent P. brasiliensis isolates. This protein may contribute to the virulence of this important fungal pathogen. Using Edman degradation and mass spectrometry analysis, this 30 kDa adhesin was identified as a 14-3-3 protein. These proteins are a conserved group of small acidic proteins involved in a variety of processes in eukaryotic organisms. However, the exact function of these proteins in some processes remains unknown. Thus, the goal of the present study was to characterize the role of this protein during the interaction between the fungus and its host. To achieve this goal, we cloned, expressed the 14-3-3 protein in a heterologous system and determined its subcellular localization in in vitro and in vivo infection models. Immunocytochemical analysis revealed the ubiquitous distribution of this protein in the yeast form of P. brasiliensis, with some concentration in the cytoplasm. Additionally, this 14-3-3 protein was also present in P. brasiliensis cells at the sites of infection in C57BL/6 mice intratracheally infected with P. brasiliensis yeast cells for 72 h (acute infections) and 30 days (chronic infection). An apparent increase in the levels of the 14-3-3 protein in the cell wall of the fungus was also noted during the interaction between P. brasiliensis and A549 cells, suggesting that this protein may be involved in host-parasite interactions, since inhibition assays with the protein and this antibody decreased P. brasiliensis adhesion to A549 epithelial cells. Our data may lead to a better understanding of P. brasiliensis interactions with host tissues and paracoccidioidomycosis pathogenesis. 相似文献
11.
Roxane M Barthélémy Anne Chenuil Samuel Blanquart Jean-Paul Casanova Eric Faure 《BMC evolutionary biology》2007,7(1):146
Background
Chaetognaths, or arrow worms, are small marine, bilaterally symmetrical metazoans. The objective of this study was to analyse ribosomal protein (RP) coding sequences from a published collection of expressed sequence tags (ESTs) from a chaetognath (Spadella cephaloptera) and to use them in phylogenetic studies. 相似文献12.
Gioconda San-Blas Felipe San-Blas Françoise Sorais Belisario Moreno José Ruiz-Herrera 《Archives of microbiology》1996,166(6):411-413
Putrescine and spermidine were the only polyamines found inParacoccidioides brasiliensis, a dimorphic fungus pathogenic for humans. Free polyamines (putrescine>spermidine) increased during the first 24 h of yeast growth, with a second peak at 42 h, and also during the first 12 h of mycelium-to-yeast transition (spermidine>putrescine). Conjugated and bound polyamines were also quantified. 1,4-Diamino-2-butanone decreased free putrescine and spermidine accumulation by inhibiting the activity of ornithine decarboxylase. The increase in free polyamines corresponds to bud emergence in yeast growth and to the mycelium-to-yeast transition ofP. brasiliensis.Abbreviations
DAB
1,4-Diamino-2-butanone
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Y
Yeasts
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M
Mycelia
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ODC
Ornithine decarboxylase 相似文献
13.
Gisela Ramos Terçarioli Eduardo Bagagli Gabriela Martins Reis Raquel Cordeiro Theodoro Sandra De Moraes Gimenes Bosco Severino Assis da Graça Macoris Virgínia Bodelão Richini-Pereira 《BMC microbiology》2007,7(1):92
Background
Paracoccidioides brasiliensis ecology is not completely understood, although several pieces of evidence point to the soil as its most probable habitat. The present study aimed to investigate the fungal growth, conidia production and molecular pathogen detection in different soil conditions. 相似文献14.
Xuchu Wang Minjing Shi Xiuli Lu Ruifeng Ma Chenggong Wu Anping Guo Ming Peng Weimin Tian 《Proteome science》2010,8(1):35
Background
Proteomic analysis of laticifer latex in Hevea brasiliensis has been received more significant attentions. However, the sticky and viscous characteristic of rubber latex as cytoplasm of laticifer cells and the complication of laticifer latex membrane systems has made it challenge to isolate high-quality proteins for 2-DE and MS. 相似文献15.
Luis González-Candelas Santiago Alamar Paloma Sánchez-Torres Lorenzo Zacarías Jose F Marcos 《BMC plant biology》2010,10(1):194
Background
Postharvest losses of citrus fruit due to green mold decay, caused by the fungus Penicillium digitaum, have a considerable economic impact. However, little is known about the molecular processes underlying the response of citrus fruit to P. digitatum. 相似文献16.
Monteiro da Silva JL Andreotti PF Benard G Soares CP Miranda ET Mendes-Giannini MJ 《Antonie van Leeuwenhoek》2007,92(1):129-135
Paracoccidioidomycosis is caused by Paracoccidioides brasiliensis, which although not formally considered an intracellular pathogen, can be internalized by epithelial cells in vitro and in
vivo. The mechanisms used by P. brasiliensis to adhere to and invade non-professional phagocytes have not been identified. The signal-transduction networks, involving
protein tyrosine kinase (PTK) and protein phosphatase activities, can modulate crucial events during fungal infections. In
this study, the involvement of PTK has been investigated in P. brasiliensis adherence and invasion in mammalian epithelial cells. A significant inhibition of the fungal invasion occurred after the
pre-treatment of the epithelial cells with genistein, a specific tyrosine kinase inhibitor, indicating that the tyrosine kinase
pathway is involved in P. brasiliensis internalization. In contrast, when the fungus was treated, a slight (not significant) inhibition of PTK was observed, suggesting
that PTK might not be the fungus’ transduction signal pathway during the invasion process of epithelial cells. An intense
PTK immunofluorescence labeling was observed in the periphery of the P. brasiliensis infected cells, little PTK labeling was found in both uninfected cells and yeast cells, at later infection times (8 and 24 h).
Moreover, when the epithelial cells were treated with genistein and infected with P. brasiliensis, no labeling was observed, suggesting the importance of the PTK in the infectious process. These results suggest that PTK
pathway participates in the transduction signal during the initial events of the adhesion and invasion processes of P. brasiliensis to mammalian epithelial cells. 相似文献
17.
Nikku L Raju Belaghihalli N Gnanesh Pazhamala Lekha Balaji Jayashree Suresh Pande Pavana J Hiremath Munishamappa Byregowda Nagendra K Singh Rajeev K Varshney 《BMC plant biology》2010,10(1):45
Background
Pigeonpea (Cajanus cajan (L.) Millsp) is one of the major grain legume crops of the tropics and subtropics, but biotic stresses [Fusarium wilt (FW), sterility mosaic disease (SMD), etc.] are serious challenges for sustainable crop production. Modern genomic tools such as molecular markers and candidate genes associated with resistance to these stresses offer the possibility of facilitating pigeonpea breeding for improving biotic stress resistance. Availability of limited genomic resources, however, is a serious bottleneck to undertake molecular breeding in pigeonpea to develop superior genotypes with enhanced resistance to above mentioned biotic stresses. With an objective of enhancing genomic resources in pigeonpea, this study reports generation and analysis of comprehensive resource of FW- and SMD- responsive expressed sequence tags (ESTs). 相似文献18.
Baozhu Guo Xiaoping Chen Phat Dang Brian T Scully Xuanqiang Liang C Corley Holbrook Jiujiang Yu Albert K Culbreath 《BMC developmental biology》2008,8(1):12
Background
Peanut (Arachis hypogaea L.) is an important crop economically and nutritionally, and is one of the most susceptible host crops to colonization of Aspergillus parasiticus and subsequent aflatoxin contamination. Knowledge from molecular genetic studies could help to devise strategies in alleviating this problem; however, few peanut DNA sequences are available in the public database. In order to understand the molecular basis of host resistance to aflatoxin contamination, a large-scale project was conducted to generate expressed sequence tags (ESTs) from developing seeds to identify resistance-related genes involved in defense response against Aspergillus infection and subsequent aflatoxin contamination. 相似文献19.
20.
Liz Valle-Aviles Shirley Valentin-Berrios Ricardo R Gonzalez-Mendez Nuri Rodriguez-del Valle 《BMC microbiology》2007,7(1):107