三种鹿茸片中氨基酸质量分数的比较

通过对东北梅花鹿茸片、新西兰鹿茸片以及东北梅花鹿茸骨片中氨基酸质量分数的比较测定,结果表明:东北梅花鹿茸片中的氨基酸质量分数高于其余两种茸片中的氨基酸质量分数,从而为合理选购和使用鹿茸片提供科学理论依据。     

二杠鹿茸与三杈鹿茸中氨基酸含量的比较

通过对二杠鹿茸与三杈鹿茸中氨基酸的测定 ,结果表明 :三杈鹿茸中的氨基酸含量高于二杠鹿茸中氨基酸含量 ,从而为合理利用二杠鹿茸、三杈鹿茸提供科学理论依据。     

梅花鹿鹿茸不同产品中氨基酸含量的比较

通过对二杠鹿茸、三杈鹿茸、鹿茸片、鹿茸血、鹿角、鹿角盘等鹿茸产品中氨基酸含量的测定研究,结果表明:鹿茸血中氨基酸含量最高,鹿茸片中的氨基酸含量次之,三杈鹿茸中的氨基酸含量高于二杠鹿茸中氨基酸含量,鹿花盘中的含量高于鹿角中的氨基酸含量,从而为鹿茸这一动物性中药材资源的功能评价、药理作用提供科学理论依据。     

带血与排血梅花鹿茸骨片游离氨基酸含量的比较分析

选用6对带血与排血梅花鹿茸骨片对其游离氨基酸的含量进行了对比测定,结果表明含有7种必需氨基酸,必需氨基酸所占比例约30%,必需与非必需游离氨基酸之比约0.4。对两者进行方差分析,两种样品游离氨基酸总量和必需游离氨基酸总量差异不显著(P(0.05)。     

提高鹿茸产量的初步试验

为了探索提高鹿茸产量的方法,我们在1973年4—7月做了初步试验,收到一定效果。现将试验结果介绍如下。 试验方法 选择体质、体型和产茸性能相差不大(见表1)的3岁梅花公鹿24头,将其中长势较好的12头,分成     

梅花鹿茸胶原酶解物对去势大鼠骨质疏松症防治作用的实验研究(英文)

观察梅花鹿茸胶原酶解物(CSDV)对去势大鼠骨质疏松症防治作用的实验研究。通过对模型组、给药组和假手术组在形态计量学指标,生物力学指标和血清生化指标的评价,考察鹿茸胶原酶解物的作用。结果表明CSDV能够明显提高去势骨质疏松症大鼠骨密度,调节血清碱性磷酸酶水平和骨钙素水平。说明鹿茸胶原酶解物在防治去势大鼠骨质疏松症方面具有明显作用。     

鲜鹿茸酶降解过程的研究

研究了胰蛋白酶、Alcalase 碱性蛋白酶、木瓜蛋白酶对鲜鹿茸的降解过程,确定了优化降解工艺条件,具有一定的理论意义和实践价值。确定了Alcalase 碱性蛋白酶的降解效率最高,通过单因素实验确定了降解过程中底物浓度、酶解温度、pH值和酶解时间为影响鲜鹿茸降解率的主要因素。正交试验确定最佳的酶解条件为:底物浓度0.08 g/ml、酶解温度65 ℃、pH 9.0、酶解时间6.0 h。在此条件下,鲜鹿茸降解率高达92.6%,氨基酸产品收率达12.1%。     

鹿茸神经再生的研究进展

在鹿茸的生长期,鹿茸对痛觉和不连续的触摸非常敏感,空间位置感也非常强,说明鹿茸含有丰富的神经系统。鹿茸作为能够完全再生的哺乳动物器官,其皮肤、血管、神经等组织成分也随之完全再生。弄清鹿茸神经再生的调控机制,将为临床上的神经损伤修复提供基础数据或有效途径。该文对鹿茸神经的结构、发生以及再生过程、再生机制等进行综述,旨在为神经损伤后的有效修复提供参考和指导。     

鹿茸再生相关蛋白研究进展

鹿茸是迄今为止发现的唯一能够周期性再生的哺乳动物器官,它的生长速度极快却没有发生癌变。而蛋白质是生命活动的体现者,是生命活动的功能执行者,鹿茸相关蛋白的研究是揭开鹿茸再生秘密的重要途径。综述了鹿茸再生相关蛋白的筛选、血管生成相关蛋白、软骨形成相关蛋白、神经再生相关蛋白以及其他与鹿茸再生相关蛋白的研究进展,旨在为从蛋白质水平上揭示鹿茸再生过程提供基础资料。     

鹿茸

指雄鹿未骨化而带茸毛的幼角。所有鹿的角每年都要脱落一次,再长更大、更粗壮的新角,80天左右成熟,每年七八月份收茸,若不及时砍茸,会老化成鹿角,药用价值降低,成熟后会自动脱落下来。鹿茸刚生出时很软,外面包着一层皮,长着许多小茸毛:里面是软骨和许多毛细血管。鹿茸中含有磷脂、糖脂、胶脂、     

White-Tailed Deer Antler Research: A Critique of Design and Analysis Methodology

ABSTRACT Debate within the popular and technical literature regarding predictability of antler size at maturity based on 1.5-year antler size in white-tailed deer (Odocoileus virginianus) has led to confusion and uncertainty within constituent groups. Koerth and Kroll (2008) provided measures of age-related antler development using recaptures of known-age males from 12 deer populations in southern Texas. Several design and analysis issues reduce the scope and validity of their conclusion that amount of growth in the first set of antlers was a poor predictor of antler growth at maturity. Although unstated, the statistical hypothesis they tested did not coincide with their specific conclusions. Using a simulation, we show that their methods were susceptible to measurement bias. Their results are applicable only to populations with similar culling and management programs. Additionally, we provide recommendations for future research projects that evaluate predictability of antler size at maturity based on antler size at younger ages.     

Expression and regulation of Angiopoietins and their receptor Tie-2 in sika deer antler

The cartilage vascularization and chondrocyte survival are essential for endochondral ossification which occurs in the process of antler growth. Angiopoietins (Ang) is a family of major angiogenic growth factors and involved in regulating the vascularization. However, the expression and regulation of Angs in the antler are still unknown. The aim of this study is to localize the expression of Ang-1, Ang-2 and their receptor Tie-2 in sika deer antler using in situ hybridization and focused on analyzing the regulation of testosterone, estrogen, all-trans-retinoic acid (ATRA) and 9cRA on their expression in antler chondrocytes. The results showed that Ang-1, Ang-2 and Tie-2 were highly expressed in antler chondrocytes. Administration of testosterone to antler chondrocytes led to a notable increase in the expression of Ang-1 and Tie-2, and a reduction in the expression of Ang-2. The similar result was also observed after estrogen treatment. In contrast, ATRA and 9cRA could inhibit the expression of Ang-1 in antler chondrocytes and heighten the expression of Ang-2. Simultaneously, ATRA could downregulate the expression of Tie-2 in antler chondrocytes at 12 and 24?h, while 9cRA upregulate the expression of Tie-2 at 3 and 6?h. Collectively, Ang-1, Ang-2 and Tie-2 are expressed in antler chondrocytes and their expression can be affected by testosterone, estrogen, ATRA and 9cRA.     

鹿茸硫酸软骨素蛋白聚糖的分离纯化研究

报道了用DEAE-纤维素(DE-23)离子交换柱层析从鹿茸二杠中分离、纯化及鉴定硫酸软骨素的方法.首先用适量蒸馏水浸泡鹿茸二杠并将其捣碎,离心取沉淀用盐酸胍浸提,浸提液对尿素液透析后经DEAE-纤维素(DE-23)离子交换柱层析,吸附大量的硫酸软骨素;再用含盐尿素溶液梯度洗脱、分离后,经软骨素酶消化及琼脂糖凝胶电泳, 与硫酸软骨素标准品比较,证实得到的物质为纯的硫酸软骨素蛋白聚糖,其得率约为48.77%.该方法使硫酸软骨素分离纯化一步完成,大大简化了纯化步骤.     

Juvenile-to-Adult Antler Development in White-Tailed Deer in South Texas

Abstract: Past studies using penned deer provide conflicting results on the age when reliable predictions about antler growth potential in white-tailed deer (Odocoileus virginianus) can be made. We captured wild whitetail males via aerial net gun on 12 ranches in 5 counties in south Texas, USA, from 1999 to 2007 to determine if a reliable juvenile-to-adult relationship in antler development existed. We individually marked and released captured animals at the trap site after we took antler and body measurements. We recaptured marked animals as possible in subsequent years or until we obtained final measurements after legal harvest. Amount of growth in the first set of antlers in whitetail males was a poor predictor of antler growth at maturity. By 4.5 years of age there were no differences (P > 0.05) in antler measurements regardless of the amount of development of the first set of antlers at 1.5 years. We concluded culling of yearling males based on number of antler points would have little positive effect on overall antler quality in future years.     

Origination of antlerogenesis

Antlers are unique appendages. They are shed and rebuilt at intervals, and are synapomorphic for all living Cervidae (except for the Chinese water deer, Hydropotes inermis , in which they have presumably been lost). The antlerogenic process is controlled by a complex interaction of fluctuating levels of several hormones, most importantly testosterone. The oldest antler remains are recorded from the early Miocene; these have been interpreted as non‐deciduous appendages because of supposed permanent skin coverage and the lack of a burr (a well‐developed osseous protuberance around the base of the antler, which is always present in extant cervids). The aim of this study is to test the hypothesis that antler shedding was possible in these early Miocene cervids. Antlers of all extant and eight Miocene cervid genera, including burr‐less antler fragments of the earliest cervids Procervulus , Ligeromeryx , and Lagomeryx were studied. An extensive comparative morphological analysis of external features of the antler, and of the abscission area and the base of the antler in particular, was undertaken. The results indicate that a regular, porous, and rugose abscission surface at the proximal end of the antler indicates antler shedding in both living and fossil cervids. The antler shedding mechanism must therefore have already been present in all early/mid Miocene cervid genera included in this study. On this basis, it is suggested that the presence of a burr is not prerequisite in order to shed antlers, that the presence of perpetual antlers has not yet been verified, and that the process of shedding and regeneration developed with the first appearance of these organs. This insight is particularly important for the systematic classification of early Miocene species as Cervidae, because the absence of the antler shedding and rebuilding mechanism would exclude them from the taxon Cervidae and from any relationship with extant cervids. J. Morphol. 278:182–202, 2017. © 2016 Wiley Periodicals,Inc.     

梅花鹿生长激素基因单核苷酸多态与产茸量性状的相关性

以梅花鹿的生长激素基因(GH)作为候选基因, 分析该基因对梅花鹿产茸量性状的影响。以吉林农业大学鹿场提供的梅花鹿为实验群体, 采用单链构象多态性(PCR-SSCP)和DNA测序的方法检测了GH基因单核苷酸多态性(SNPs), 针对该群体的特点建立合适的统计分析模型, 并进行了GH基因多态性与产茸量的相关分析。结果表明, GH基因对梅花鹿的产茸量有一定影响。G→A突变产生的3种基因型间的第五锯产茸量存在一定的差异(P<0.2), BB基因型个体在第五锯的产茸量与AA基因型个体之间有一定的差异(P<0.2)。