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1.
用RAPD方法分析水稻光敏核不育基因   总被引:31,自引:0,他引:31  
本文以农垦58S与F2杂交所得的F2分离群体为材料,按其育性分为不育和可育两大群体,分别以混合的可育群体核DNA和不育体核DNA为模板,进行RAPD分析,从检测过的300个引物中发现有2个引物在不育群体和可育群体中扩增出多态性产物。就其中一个引物对杂交亲本和F2个体的RAPD分析进一步证明了这种多态可靠性。转移杂交实验表明这个多态性产物是一种重复顺序。  相似文献   

2.
李传友  伏健民 《遗传学报》1999,26(5):558-562
85EA是通过电子束辐照获得的胞质突变型小麦不育要用RFLP和RAPD技术对85EA及春保持系的线粒体DNA进行了比较研究。RFLP分析表明85EA线粒体基因组中coxⅡ基因的位置结构与保持系发生了变化;RAPD分析中引物OPD-15扩增产物在不育系和保持系间有明显差异,不育系的扩增产物比保持系多1条分子量为0.6kb的特展览 要带,用T-easy vector克隆该不育系特异条带并命名为OPD-  相似文献   

3.
利用分子标记定位农垦58S的光敏核不育基因   总被引:17,自引:0,他引:17  
对农垦58S(Oryzasativasp.japonica)/大黑矮生标记基因系FL2组合组建可育集团和不育集团,并以亲本为对照进行了RFLP、RAPD和双引物RAPD分析,结果第12染色体上的一个单拷贝标记G2140与光敏核不育基因连锁遗传,二者间的遗传图距为14.1cM(centimorgan)。在筛选过的1040个随机单引物和190个双引物中,仅引物OPAU10扩增出与光敏核不育基因连锁的1.5kbDNA片段,回收、克隆该DNA片段并制备探针,将其转换成共显性的RFLP标记并命名为OPAU101500。分离群体连锁分析表明该标记与标记G2140紧密连锁,将农垦58S的一对光敏核不育基因定位于第12染色体上。  相似文献   

4.
鹅膏菌属真菌RAPD分析及亲缘关系的研究   总被引:14,自引:0,他引:14  
本文对采自湖南莽山的26种鹅膏菌属(Amanita)真菌进行了随机扩增多态性DNA(RAPD)分析,40个随机引物中筛选出扩增效果较好的6个引物,每个引物能产生1~10条DNA条带,获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来,通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20-60%之间,平均链锁聚类分析  相似文献   

5.
冬虫夏草的随机扩增多态DNA及其遗传分化   总被引:25,自引:0,他引:25  
陈永久  杨跃雄 《遗传学报》1997,24(5):410-416
本文对来自青藏高原3个区域5个具有代表性地方的13个冬虫夏草样本进行随机扩增多态DNA(RAPD)分析。19个随机引物获得的RAPD谱带清晰并呈现多态,单个引物获得的RAPD片段数在3 ̄10个之间。该19个引物在每个样本中扩增的RAPD片段总数平均约为65个。基于遗传距离分析,受试的13个冬虫夏草样本中,来自同一地方的样本间遗传差异甚微,同一区域不同地方的样本间遗传差异较大,不同区域的样本间遗传差  相似文献   

6.
本文对采自湖南莽山的26种鹅膏菌属(Amanita)真菌进行了随机扩增多态性DNA(RAPD)分析,40个随机引物中筛选出扩增效果较好的6个引物,每个引物能产生1~10条DNA条带,获得的RAPD谱带清晰并呈现多态性OPG15、OPH04两个引物扩增的RAPD谱带能将26种鹅膏菌完全区分开来,通过6个引物的RAPD分析获得的平均相似性系数表明种与种之间的相关系数在20-60%之间,平均链锁聚类分析可将26种鹅膏菌分为二大类,且一些具菌环和苞状菌托的种类聚在一起,与形态分类基本相吻合。  相似文献   

7.
小麦耐盐突变体的RAPD分析   总被引:4,自引:0,他引:4  
以冀麦24和其耐盐的一代8901-17为材料,用RAPD技术对其进行比较分析研究。在的用的215个引物中有51个扩增出多态性,既有量的差异又有质的差异,初步断定此51个引物扩增出的多态性与突变有关,为进一步用BSA方法细筛出与耐盐突变体紧密连锁的RAPD分子标记下打下基础,同时,还分析了影响RAPD扩增结果的因素。  相似文献   

8.
中国紫花苜蓿地方品种随机扩增多态DNA的研究   总被引:16,自引:0,他引:16       下载免费PDF全文
运用RAPD技术,对11个样本紫花苜蓿(Medicago sativa)的遗传多样性进行了研究。计算了11个样本之间的遗传相似度和遗传距离,并进行了聚类分析,探讨了它们之间的亲缘关系。结果如下:1.用43个随机引物对紫花苜蓿的11个样本的核DNA进行了RAPD扩增,共检出440条扩增片段,多态性片段331条,占70.68%。反映了11个样本之间的遗传变异;2.43个随机引物对11个样本扩增出2~1  相似文献   

9.
以随机扩增多态DNA技术(RAPD)分析了奥利亚罗非鱼和尼罗罗非鱼两个养殖群体的群体内及群体间遗传关系,并探讨了该技术在种群鉴定中的应用。RAPD引物筛选结果表明,所测试的20个随机引物中(Table 1),除一个引物未扩增出任何片段外,其余19个引物均扩增出1~11个大小不等的片段,长度大部分在500~3000bp之间,共扩增出220个片段,平均每个引物产生5.5个片段。两群体间共有片段70条,大部分引物的扩增产物具有种间多态性,种群间相似系数为0.727。以筛选的引物对两种群不同个体(Fig.1,Table2)及种群混合样品(Fig.2,Table3)进行RAPD分析。结果表明,不同引物在扩增图谱上表现很大差异。奥利亚罗非鱼不同个体间表现为一致的扩增图谱,种内相似系数达1000,显示了其种群内遗传变异的缺乏;尼罗罗非鱼种内相似系数为0.827,个体间存在不同程度的多态性;两个种群间的相似系数分别为0.767和0.742,表明种间有较高的同源性,遗传距离为0.235,略低于国外的报道、此外,两个养殖群体间的扩增图谱比较也暗示了遗传渐渗现象的存在。实验表明,RAPD标记可以作为一种可靠的遗传标记,用于不同鱼  相似文献   

10.
应用随机扩增多态DNA(Random amplified polymorphic DNAs,RAPD)技术,对普通小麦细胞质雄性不育系(A)及其保持系(B)线粒体基因组(mitochondrial genome)的指纹图谱进行了分析。共使用引物41个,其中20个引物得到了扩增产物,部分引物扩增结果表现多态性。说明线粒体DNA(mitochondrial DAN,mt,DNA)序列在不育系和保持系之  相似文献   

11.
RAPD和ISSR标记对水稻化感种质资源遗传多态性的分析   总被引:23,自引:1,他引:22  
运用RAPD和ISSR技术分析水稻化感种质资源的遗传多态性。从供试材料中筛选到具有多态性的RAPD引物12条,ISSR引物7条。RAPD引物共扩增到85条清晰的多态性条带,多态性条带比率为69.4%。ISSR引物共扩增到34条清晰的多态性条带,多态性条带比率为53.0%。对两种标记结果进行UPGMA聚类分析,结果极其类似,呈极显著的正相关(r=0.74)。聚类结果表明,地理位置相近的品种聚为一类。部分具有较强化感作用潜力的水稻品种亲缘关系很近,表明控制其化感作用性状的基因可能是等位的相同基因。而部分化感作用潜力差异显著的水稻品种聚为一类,这是由于人类在长期高产品种的定向选择过程中,水稻化感作用性状不被注意而丢失,遗传基础日益狭窄的原因。  相似文献   

12.
The technique of random amplified polymorphic DNA (RAPD) offers a broad range of applications in the investigation of plant genomes. A promising prospect is the use of RAPD products as genetic markers. We have investigated a possible organellar source of fragments in RAPD patterns of total DNA. Two nearly-isogenic lines of cytoplasmic male-sterile and male-fertile sugar beet (Beta vulgaris L.) were subjected to RAPD analysis with six different primers. Total, nuclear, mitochondrial (mt), and chloroplast (cp), DNA from each line were investigated. Reproducible DNA fingerprints could be obtained from both organellar DNAs. Differences in band patterns of mtDNA between cytoplasmic male-sterile and -fertile lines were observed with five out of six primers, whereas different cpDNA patterns were generated by one of the primers. Consequently, the RAPD technique can be used to discriminate between different cytoplasms. Clear evidence is provided for the organellar origin of fragments in genomic (total DNA) RAPD patterns. The consequences of these results for the interpretation of RAPD analyses are discussed.  相似文献   

13.
利用RAPD对稻蝗属昆虫亲缘关系的研究   总被引:19,自引:4,他引:15  
通过20个随机引物的PCR扩增,得到了日本主要稻蝗的随机扩增多态性DNA(RAPD)图谱,根据扩增结果,计算了种间相似系数和遗传距离,建立了UPGMA系统树。结果表明,分布没有重叠、种间容易交配、能产生杂种的中华稻蝗台湾亚种与小翅稻蝗的亲缘关系最近;分布重叠的日本稻蝗与中华稻蝗台湾亚种、日本稻蝗和小翅稻蝗的亲缘关系较近。小稻蝗与其它3种稻蝗的亲缘关系较远。  相似文献   

14.
Neutral and pathogenicity markers were used to analyse the population structure of Magnaporthe grisea rice isolates from the north‐western Himalayan region of India. Random amplified polymorphic DNA (RAPD)‐based DNA fingerprinting of 48 rice isolates of M. grisea with five primers (OPA‐04, OPA‐10, OPA‐13, OPJ‐06 and OPJ‐19) showed a total of 65 RAPD bands, of which 54 were polymorphic. Cluster analysis of 48 rice isolates of M. grisea on the basis of these 65 RAPD bands revealed the presence of high genotypic diversity and continuous DNA fingerprint variation in the pathogen population. No correlation was observed between RAPD patterns and virulence characteristics of the pathogen. The observed population structure contrasted with presumed clonal reproductive behaviour of the pathogen and indicated the possibility of ongoing genetic recombination in the pathogen population. Analysis of the virulence organization of five RAPD groups (RG1–RG5) using 20 rice genotypes comprising at least 15 resistance genes revealed that no combination of resistance genes would confer resistance against all RAPD fingerprint groups present in the M. grisea rice population. The possible implications of the observed population structure of M. grisea for blast resistance breeding have been discussed.  相似文献   

15.
16.
BT型细胞质雄性不育水稻及其三系的线粒体DNA研究   总被引:2,自引:0,他引:2  
用RAPD技术对BT型水稻胞质雄性不育系秀A及其保持系秀B、恢复系湘晴以及杂种F1代的线粒体DNA进行了比较分析。结果表明不育系与其保持系间存在显著差异;不育系与其F1之间mtDNA也存在差异。在引物OPJ-08的扩增产物中,秀A扩增出一条分子量为800bp的多态性片段,在引物OPK-10的扩增产物中,杂种F1扩增出一条分子量为900bp的片段。把这两片段回收、克隆并制备探针,OPJ-08800的Southern杂交结果显示不育系与其F1杂交图谱存在多态性;OPK-10900的Suthern杂交结果显示不育系与其保持系同存在差异。推测这两片段与育性可能有一定的联系。  相似文献   

17.
Thirty Italian isolates of the phytopathogenic fungus Ascochyta rabiei (Pass.) Labr., the causal organism of Ascochyta blight on chickpea (Cicer arietinum L.), were analysed by a random oligonucleotide primer dependent polymerase chain, reaction (PCR) technique called random amplified polymorphic DNA analysis (RAPD) using three decamer primers. In previous investigations these isolates had been differentiated in six pathogenic groups. RAPD results were summarized in an analysis using the program PAUP. With each of the primers several amplification products were observed which were common to all isolates. The results of the RAPD analyses also showed that all isolates could be identified by a unique RAPD pattern. No correlation between RAPD patterns and the division of the isolates in pathogenic groups could be established. The application of the RAPD technique for cataloguing isolates and to obtain specific genetic markers for all isolates of the species Ascochyta rabiei is discussed.  相似文献   

18.
RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis , up to 10 strain types could be determined when two 10-mer primers were used. RAPD patterns, which were found in eight further strains of Bacillus spp., clearly differed from those of B. licheniformis. Thus RAPD technique proved to be a promising tool for characterization of Bacillus spp.  相似文献   

19.
Genetic variation between samples of Oryza sativa from 19 localities in Bangladesh and Bhutan was assessed using two PCR-based molecular marker systems: RAPD (random amplification of polymorphic DNA) and ISSR-PCR (inter-simple sequence repeat polymerase chain reaction). Employing RAPD, a set of 14 decanucleotides of arbitrary sequence directed the amplification of 94 reproducible marker bands, 47 (50%) of which were polymorphic. In addition, a set of 9 ISSR primers were used to direct amplification of 71 PCR products, 40 (56%) of which were polymorphic. Multivariate analyses of the two PCR-based molecular marker data sets provided evidence that the patterns of variation correspond with the classification described by Glaszmann [9] using isozyme analysis. Subtle differences in the relationships revealed between rice groups using the two types of PCR-based marker led to investigations of their map positions using an intraspecific doubled haploid mapping population. The observation that the chromosomal locations of markers can influence diversity assessments is presented and the significance of this is discussed.  相似文献   

20.
红莲型杂交稻(红莲2号)及其骨干亲本的RAPD分析与鉴定   总被引:4,自引:0,他引:4  
利用RAPD技术,从248个随机寡核苷酸引物(10-mer)中筛出18个引物对红莲型杂交稻组合红莲2号及其亲本(T-07A、T-07B、YD6-05),另6个红莲型胞质不育系的骨干恢复和汕优63及其亲本共14份水稻材料进行分析。共检测到173个多态性标记。聚类分析结果表明:不育系与保持系间因核背景相似,遗传差异很小;杂种(F1)的基因型更倾向于恢复系;恢复系与保持系间遗传距离的相对较大,但各恢复系之间的遗传距离较小。利用这些标记能有效地地区交组合中不育系,保持系、恢复系和杂种(F1)。  相似文献   

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