共查询到19条相似文献,搜索用时 31 毫秒
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本文研究了水分胁迫下益智植株过氧化物酶活性、叶绿素含量、脯氨酸含量和总水量的与抗旱性的相关。结果表明,益智受干旱时不同部位过氧化物酶活性与脯氨酸含量变化呈波动状升高,叶绿素含量与总水量有不同程度降低的趋势。 相似文献
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以小麦双单倍体(DH)群体(旱选10号×鲁麦14)的150个株系及其亲本为材料,对不同处理条件下的胚芽鞘长、幼苗株高和幼苗生物学产量进行遗传分析,以探讨胚芽鞘长、幼苗株高及幼苗生物学产量的遗传基础及其相关性.结果表明:(1)随着播种深度的增加,胚芽鞘长、幼苗株高显著增加,16.1%PEG-6000处理条件下的幼苗株高显著低于对照,干旱胁迫条件下的幼苗生物学产量显著低于正常供水对照;(2)胚芽鞘长、幼苗株高、幼苗生物学产量的遗传力分别为92.5%、90.3%和78.1%;(3)胚芽鞘长、幼苗株高及幼苗生物学产量均受多基因控制,决定各性状的基因估计数目分别为7、8和14对;(4)根据对性状偏度和峰度系数的估算结果,控制这3个性状的多基因之间均有互补作用;胚芽鞘长与幼苗株高、生物学产量及成熟期株高之间呈显著正相关. 相似文献
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果胶裂解酶是果胶酶的重要成员之一,能够通过B-消除作用,催化降解(1—4)-α-D-聚半乳糖醛酸。本研究首次鉴定出禾谷镰孢茵中的一个果胶裂解酶PelA,并在体外检测了其生化特性。PelA基因(FGSG_02386)的cDNA被克隆并进行了原核表达,同时构建了单基因敲除突变体。将重组质粒pET-28a(+)-PelA转化蛋白表达菌株大肠杆菌BL21表达目的蛋白,纯化后的蛋白具有果胶裂解酶活性。酶活性测定结果显示,该酶的活性受到外界环境因素如温度、钙离子浓度和pH条件的影响,其最适反应条件为50℃,CaCl:浓度0-5mmol·L^-1,pH8-5。在侵染小麦胚芽鞘时PelA单基因突变体的毒力较野生型菌株并没有出现明显变化。 相似文献
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Grald Perbal Dominique Driss-Ecole 《Biology of the cell / under the auspices of the European Cell Biology Organization》1988,62(3):275-280
Tritiated auxin applied by an agar block on the wheat coleoptile tip for 2 hr was covalently fixed to adjacent protein by treatment with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (DCC). The density of labelled auxin in the nucleus, the cell wall, the cytoplasm, and the vacuole was determined by autoradiography. Localization of tritiated auxin was studied at high resolution at the tonoplast and the plasmalemma lining the transverse (distal and proximal) and the longitudinal walls. The radioactivity along the tonoplast was always less than along the plasma membrane. The distribution of 3H-auxin was different across the longitudinal and transverse regions of the plasmalemma. The labelling was distributed asymmetrically on the longitudinal plasma membrane with a peak observed on the external surface. Tritiated auxin was distributed more symmetrically on the distal and the proximal plasma membranes. Our results are in agreement with the hypothesis that there are 2 different specific binding sites on the plasmalemma. The ratio of auxin present at the proximal and distal regions of the plasmalemma was 1.28. 相似文献
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Ultrastructure of wheat coleoptile mitochondria at short-term anoxia and post-anoxia 总被引:3,自引:0,他引:3
Abstract. Mitochondrial ultrastructure in the cells of coleoptiles of 4 d seedlings was investigated under conditions of a 1.5, 3, and 36 h anoxia and with subsequent transfer of the seedlings, after a 1.5 h anoxia, from the anaerobic into an aerobic medium. Even with short-term anoxia (1.5 h) destructive changes take place in the ultrastructure of mitochondria, which are reversible not only following the transfer of these seedlings from the anaerobic into aerobic conditions, but also with their continued maintenance under strict anoxia. Irreversible changes in the ultrastructure of mitochondria were seen only with a more prologed (36 h and longer) anoxia. The observed phenomena are discussed from the viewpoint of energy provision of the seedling cells in anoxia and post-anoxia. 相似文献
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激光—核辐射诱变小麦后代相关遗传力的分析 总被引:1,自引:0,他引:1
本试验采用He-Ne和N2激光辐照“汉原小麦”等四个材料的干种子,采用随机区组设计,重复三次,利用生物统计学和数量遗传学的方法,从个水平上对激光-核辐射小麦L2代抽穗期等19个性状的相关遗传力分析表明:利用株高间接选择结实小穗数、旗叶长、小穗密度和有效分蘖数。 相似文献
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外源IAA 处理可以显著增加小麦胚芽鞘细胞NAD 激酶的催化活性,钙离子可以增强IAA 的作用效果,而钙离子通道抑制剂LaCl3 则起强烈的抑制作用,但在存在钙离子的条件下,这种抑制作用可以被钙离子载体A23187 消除;钙调蛋白能够在离体条件下激活经过DEAE 纤维素柱纯化的小麦胚芽鞘NAD激酶,经过IAA 处理的胚芽鞘细胞中能够刺激NAD 激酶活性的钙调蛋白含量明显增加,IAA 的这一作用受LaCl3 的抑制。上述结果表明Ca2+ /CaM 复合物介导了生长素对小麦胚芽鞘细胞NAD 激酶活性的促进作用。 相似文献
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渗透胁迫对小麦胚芽鞘内多胺的种类、形态和含量的影响 总被引:6,自引:0,他引:6
用高压液相色谱法研究了豫麦18(抗旱性较强)和扬麦9号(抗旱性较弱)小麦胚芽鞘中三种不同形态的多胺(polyamine,PA):游离态多胺(PA)、高氯酸可溶性结合态多胺(ps结合态PA)和高氯酸不溶性结合态多胺(PIS结合态PA)与渗透胁迫的关系。结果发现:渗透胁迫2d,豫麦18胚芽鞘中的游离态Spd和游离态Spm的含量明显上升,而扬麦9号的游离态Put的上升明显。S-腺苷蛋氨酸脱羧酶(S—AMDC)的抑制剂——甲基乙二醛-双(鸟嘌呤腙)(MGBG)处理豫麦18,明显抑制了渗透胁迫诱导的游离态Spd和游离态Spm的增加,并且加重了渗透胁迫伤害,外源Spd处理扬麦9号明显促进了渗透胁迫诱导的游离态Spd和游离态Spm的增加,并且减缓了渗透胁迫的伤害。渗透胁迫下,豫麦18胚芽鞘中的PS结合态PA和PIS结合态PA的上升幅度都明显大于扬麦9号。菲咯啉(o—Phen)抑制渗透胁迫下PIS结合态PA的合成并加重了渗透胁迫对胚芽鞘的伤害。这些结果表明:小麦胚芽鞘中的游离态Spd、游离态Spm、PS结合态PA和PIS结合态PA的升高有利于增强渗透胁迫抗性。 相似文献
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Structural features of noncellulosic β-d-glucans of Zea mays, Hordeum vulgare, Triticum vulgare, Secale cereale, and Sorghum bicolor were compared. Treatment of cell walls derived from these species with specific Bacillus subtilis or Rhizopus glucanases yields virtually identical profiles upon Bio-Gel P-2 fractionation of the liberated oligosaccharides. The two predominant reaction products, a trisaccharide and tetrasaccharide, were identified as 3-O-β-cellobiosyl-d-glucose and 3-O-β-cellotriosyl-d-glucose respectively by virtue of the specificity of these enzymes and by paper chromatography and electrophoresis. The similarity of the reaction product profiles indicates a rather regular repeating sequence in all β-d-glucans examined. The ratios of 3-O-β-cellobiosyl-d-glucose to 3-O-β-cellotriosyl-d-glucose indicates that 30.4–30.9% of the β-glucosyl linkages in the intact molecule are 1 → 3. The yields of wall glucan as estimated from the quantity of oligosaccharides released, range from 41 μg/mg wall in Hordeum to 97 μg/mg wall from Sorghum. 相似文献