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1.
Betelvine (Piper betle L., family Piperaceae) is an important, traditional and widely cultivated crop of India. The cultivators and consumers recognize
more than 100 cultivars (landraces) based on regional and organoleptic considerations, while in terms of phytochemical constituents
only five groups have been identified for all the landraces. Since betelvine is an obligate vegetatively propagated species,
genomic changes, if any, may have become ‘fixed’ in the landraces. We carried out random amplified polymorphic DNA (RAPD)
analysis in several landraces considered in four groups, namely, ‘Kapoori’, ‘Bangla’, ‘Sanchi’ and ‘Others’ in order to ascertain their genetic diversity. On the basis of the data from eleven RAPD primers, we distinguished genetic
variation within and among the four groups of landraces. The results indicate the’Kapoori’ group is the most diverse. The neighbour joining (NJ) tree after a bootstrap (500 replicate) test of robustness clearly shows
the four groups to be well separated. Interestingly, all known male or female betelvine landraces have separated in the NJ
tree indicating an apparent gender-based distinction among the betelvines. 相似文献
2.
V. R. Bommineni P. P. Jauhar T. S. Peterson R. N. Chibbar A. B. Almouslem 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):757-763
The objective of this study was to detect the presence of alien chromatin in intergeneric hybrids of durum wheat (Triticum turgidum, 2n=4x=28; AABB genomes) with the perennial grass Thinopyrum junceiforme (2n=4x=28; J1J1J2J2) using RAPD markers. The first step was to identify amplification of species-specific DNA markers in the parental grass species
and durum wheat cultivars. Initially, the genomic DNA of five grass species (Thinopyrum junceiforme, Th. bessarabicum, Lophopyrum elongatum, Leymus karataviensis and Elytrigia pycnantha) and selected durum cultivars (‘Langdon’, ‘Durox’, ‘Lloyd’, ‘Monroe’, and ‘Medora’) was screened with 40 oligonucleotide
primers (nano-mers). Three oligonucleotides that amplified DNA fragments specific to a grass species or to a durum cultivar
were identified. Primer PR21 amplified DNA fragments specific to each of the five durum cultivars, and primers PR22 and PR23
amplified fragments specific to each of the grass species. Intergeneric hybrids between the durum cultivars ‘Langdon’, ‘Lloyd’
and ‘Durox’ and Th. junceiforme, and their backcross (BC) progeny were screened with all 40 primers. Six primers amplified parent-specific DNA fragments
in the F1 hybrids and their BC1 progeny. Three primers, PR22, PR23 and PR41, that amplified Th. junceiforme DNA fragments in both F1 and BC1 were further analyzed. The presence of an amplified 1.7-kb Th. junceiforme DNA fragment in the F1 hybrids and BC1 progeny was confirmed using Southern analysis by hybridization with both Th. junceiforme genomic DNA and Th. junceiforme DNA amplified with primer PR41. With the exception of line BC1F2 no. 5, five selfed progeny of BC1 and a BC2 of line 3 (BC1F2 no. 3בLloyd’) from a cross of ‘Lloyd’×Th. junceiforme showed the presence of the 1.7-kb DNA fragment. All selfed BC1 and BC2 lines retained the 600-bp fragment that was confirmed after hybridization with Th. junceiforme DNA amplified with primer PR22. Other experiments using RFLP markers also showed the presence of up to seven Th. junceiforme DNA fragments in the F1 hybrids and their BC progeny after hybridization with Th. junceiforme DNA amplified with primer PR41. These studies show the usefulness of molecular markers in detecting alien chromatin/DNA fragments
in intergeneric hybrids with durum wheat.
Received: 21 November 1996 / Accepted: 21 March 1997 相似文献
3.
Zabihollah Zamani Ali Shahi-Gharahlar Reza Fatahi Naser Bouzari 《Plant Systematics and Evolution》2012,298(2):499-509
Subgenus Cerasus species are useful genetic resources for cherry breeding programs. A total of 17 morphological traits together with 19 random
amplified polymorphic DNA (RAPD) primers were used to study 39 accessions including 34 wild Cerasus subgenus genotypes belonging to Prunus avium L., P. cerasus L., P. mahaleb L., P. microcarpa Boiss., P. incana Pall., and P. brachypetala Boiss. species, along with an unknown wild Cerasus sample, two advanced cherry cultivars (‘Lambert’ and ‘Bulgar’), and two rootstocks (‘Colt’ and ‘Gisela 6’). Genotypes were
separated into different groups according to their species and collection sites using cluster analysis performed by Ward’s
clustering method based on morphological data. Nineteen RAPD primers from 60 screened produced 304 polymorphic reproducible
bands (98.15% polymorphism). According to the similarity matrix, the lowest similarity was obtained between P. avium and P. microcarpa samples. A dendrogram was prepared by the unweighted pair-group method with arithmetic average (UPGMA), and the accessions
were separated according to their species and geographic origin. In both morphological and molecular results, the advanced
cultivars and rootstocks were separated from wild genotypes, and the unknown genotype was grouped with P. mahaleb accessions. Grouping by morphological characteristics was compared with the results of RAPD analysis, with no significant
correlations between morphological and molecular data being found. This is the first report of molecular (RAPD) genetic diversity
study in wild Cerasus subgenus genotypes from Iran, and the results demonstrate the high potential of RAPD analysis for discrimination of Cerasus subgenus genotypes. 相似文献
4.
Otakar Rop Jiri Mlcek Tunde Jurikova Magdalena Valsikova 《Central European Journal of Biology》2012,7(4):672-679
At present, Cape gooseberry (Physalis peruviana) fruit is one of the less used raw materials of plant origin, which can be used for human nutrition. This fruit, as well
as alimentary products made of it, were used by healers in folk medicine in the distant past. The aim of this study was to
monitor and evaluate the antioxidant capacity of fresh fruit of three Cape gooseberry cultivars ‘Giant’, ‘Golden berry’ and
‘Inka’. Antioxidant capacity was also tested, on the basis of the scavenging effect of reactive oxygen species (ROS) and lipid
peroxidation of methanolic extracts made of fresh fruit. These results were further extended and supplemented with determinates
of the vitamin C and total phenolic contents. These analyses were made for three consecutive years. The highest values of
antioxidant capacity were observed in the ‘Inka’ cultivar (9.31 grams of ascorbic acid equivalents kg−1 of fresh mass). In this cultivar, the obtained results were corroborated also in ROS and the contents of vitamin C and total
phenolics. Due to a high antioxidant capacity of this fruit species, the results presented should increase its popularity
above all as a promising raw material, which can be used for human nutrition. 相似文献
5.
Márta Molnár-Láng András Cseh Éva Szakács István Molnár 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(8):1535-1545
The main objective of the present work was to develop a wheat genotype containing both the recessive crossability alleles
(kr1kr1kr2kr2), allowing high crossability between 6x wheat and diploid rye, and the 1BL.1RS wheat/rye translocation chromosome. This wheat
genotype could be used as a recipient partner in wheat–rye crosses for the efficient introduction of new allelic variation
into 1RS in translocation wheats. After crossing the wheat cultivars ‘Mv Magdaléna’ and ‘Mv Béres’, which carry the 1BL.1RS
translocation involving the 1RS chromosome arm from ‘Petkus’, with the line ‘Mv9 kr1’, 117 F2 plants were analysed for crossability, ten of which had higher than 50% seed set with rye and thus presumably carried the
kr1kr1kr2kr2 alleles. Four of the ten plants contained the 1BL.1RS translocation in the disomic condition as detected by genomic in situ
hybridization (GISH). The wheat × rye F1 hybrids produced between these lines and the rye cultivar ‘Kriszta’ were analysed in meiosis using GISH. 1BL.1RS/1R chromosome
pairing was detected in 62.4% of the pollen mother cells. The use of fluorescent in situ hybridization (FISH) with the repetitive
DNA probes pSc119.2, Afa family and pTa71 allowed the 1R and 1BL.1RS chromosomes to be identified. The presence of the 1RS
arm from ‘Kriszta’ besides that of ‘Petkus’ was demonstrated in the F1 hybrids using the rye SSR markers RMS13 and SCM9. In four of the 22 BC1 progenies analysed, only ‘Kriszta’-specific bands were observed with these markers, though the presence of the 1BL.1RS translocation
was detected using GISH. It can be concluded that recombination occurred between the ‘Petkus’ and ‘Kriszta’ 1RS chromosome
arms in the translocated chromosome in these plants. 相似文献
6.
Leal F Loureiro J Rodriguez E Pais MS Santos C Pinto-Carnide O 《Plant cell reports》2006,25(9):978-985
Flow cytometry was employed to determine the ploidy level of Vitis vinifera L. somatic embryo-derived plants obtained from anther culture. Only one among the 41 analysed plants (2.4%) presented somaclonal variation (tetraploidy); the other plants were diploid. No significant differences (P≤0.05) were detected between diploid and parental field plants. No haploid or aneuploid plants were observed. The nuclear DNA content of nine V. vinifera cultivars was also estimated using flow cytometry. A non-significant variation was found among the cultivars, with DNA content ranging from 1.17 pg/2C (cv. ‘Tinta Barroca’ and ‘Viosinho’) to 1.26 pg/2C (cv. ‘Cabernet Sauvignon’). These results and previous studies on other Vitis species suggest that Vitis genome is stable with regard to nuclear DNA content. 相似文献
7.
Pâmela Fávero Francisco de Assis Alves Mourão Filho Liliane Cristina Libório Stipp Beatriz Madalena Januzzi Mendes 《Acta Physiologiae Plantarum》2012,34(2):471-477
The difficulty in adult tissue genetic transformation in woody species is still an obstacle to be overcome, including in most
sweet orange cultivars of the Brazilian citrus industry. This work reports that, after in vitro culture adjustments, transgenic
adventitious buds of ‘Hamlin’, ‘Pêra’, and ‘Valencia’ sweet oranges (Citrus sinensis L. Osbeck) were recovered using adult material as explant source, in genetic transformation experiments via Agrobacterium tumefaciens. The transgenic buds were identified by the GUS histochemical analysis and confirmed by PCR analysis, which indicated the
presence of an amplified fragment of 817 bp corresponding to the uidA gene sequence. The efficiencies of genetic transformation for ‘Hamlin’, ‘Pêra’, and ‘Valencia’ sweet orange cultivars were
2.5, 1.4, and 3.7%, respectively. Media supplemented with auxins and cytokinins during co-culture, and media with high concentrations
of cytokinins (3 mg L−1) during transgenic selection led to the transformation and, consequently, the regeneration of adequate number of adventitious
buds for the three cultivars. The use of sonication during the explant disinfection was not effective to reduce endophytic
contamination and reduced transformation efficiency. 相似文献
8.
This study analyzed genetic differences of 19 cultivars selected from somaclonal variants of Syngonium podophyllum Schott along with their parents as well as seven additional Syngonium species and six other aroids using amplified fragment length polymorphism (AFLP) markers generated by 12 primer sets. Among the 19 somaclonal cultivars, ‘Pink Allusion’ was selected from ‘White Butterfly’. Tissue culture of ‘Pink Allusion’ through organogenesis resulted in the development of 13 additional cultivars. Self-pollination of ‘Pink Allusion’ obtained a cultivar, ‘Regina Red Allusion’, and tissue culture propagation of ‘Regina Red Allusion’ led to the release of five other cultivars. The 12 primer sets generated a total of 1,583 scorable fragments from all accessions, of which 1,284 were polymorphic (81.9%). The percentages of polymorphic fragments within ‘White Butterfly’ and ‘Regina Red Allusion’ groups, however, were only 1.2% and 0.4%, respectively. Jaccard's similarity coefficients among somaclonal cultivars derived from ‘White Butterfly’ and ‘Regina Red Allusion’, on average, were 0.98 and 0.99, respectively. Seven out of the 15 cultivars from the ‘White Butterfly’ group and three out of six from the ‘Regina Red Allusion’ group were clearly distinguished by AFLP analysis as unique fragments were associated with respective cultivars. The unsuccessful attempt to distinguish the remaining eight cultivars from the ‘White Butterfly’ group and three from the ‘Regina Red Allusion’ group was not attributed to experimental errors or the number of primer sets used; rather it is hypothesized to be caused by DNA methylation and/or some rare mutations. This study also calls for increased genetic diversity of cultivated Syngonium as they are largely derived from somaclonal variants. 相似文献
9.
Volker Wissemann Friederike Gallenmüller Christiane Ritz Tina Steinbrecher Thomas Speck 《Trees - Structure and Function》2006,20(3):340-347
Within Rosa section Caninae (dogroses) two different growth types exist, arching (L-type) and erect (D-type). Due to the specific mode of chromosome allocation during meiosis, character inheritance in dogroses is maternally skewed. In this paper we try to assess the mode of inheritance of the ‘growth form’ in reciprocal hybrids of R. canina L. and R. rubiginosa L. to predict possible ecological niches and radiation processes of hybridogenic offspring. A quantitative analysis of mechanical properties indicates that neither an expected parentally skewed nor an intermediate habit was expressed by the hybrids, but a dominant L-type as in R. canina, regardless of the parental relationship (pollen-parent or seed-parent). Our results implicate two possible scenarios to be further tested: (a) R. canina acts as a species with a dominant allele for the growth form character. If so, this would be the first evidence of a Mendelian character inheritance in the dogroses. (b) ‘Growth form’ in the dogroses is not a Mendelian character, but a syndrom which realises as an emergent functional property with underlying phenotypic structural differences in stem and wood anatomy.The first two authors contributed equally to the study. 相似文献
10.
S. Jayasankar Richard E. Litz Raymond J. Schnell Andres-Cruz Hernandez 《In vitro cellular & developmental biology. Plant》1998,34(2):112-116
Summary Genomic DNA isolated from embryogenic cultures of two mango cultivars, ‘Hindi’ and ‘Carabao,’ that had been selected for resistance
to the culture filtrate ofColletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD).In vitro selection caused changes in RAPD markers in the selected embryogenic cultures with respect to the unchallenged control cultures
and the stock plants. The differences involved both the absence and the presence of additional RAPD markers in the resistant
lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either
cultivar and DNA from the leaves of parent trees confirmed that the changes were not due to prolonged maintenance in liquid
cultures. 相似文献
11.
Monica M. Miazzi Hajaj R. Hajjeh Francesco Faretra 《In vitro cellular & developmental biology. Plant》2010,46(4):363-367
Powdery mildew, caused by the obligate biotrophic ascomycete Erysiphe necator, is one of the most destructive grapevine diseases worldwide. Cultivars of Vitis vinifera L, for wine and table grape production, are all susceptible to E. necator, whose attacks result in severe epidemics under the warm and dry conditions of the Mediterranean basin. The aim of the present
study was to compare the susceptibility of different grapevine cultivars to E. necator by an in vitro assay for assessing the potentiality of this method in breeding programs for resistance to the pathogen. Leaves of 12 grapevine
cultivars were spot-inoculated in vitro with about 10 conidia from five different isolates of E. necator, using colony growth and conidiation 3 wk post-inoculation as indicators of susceptibility to the disease. A remarkable difference
was observed between highly susceptible cultivars like ‘Baresana’, ‘Malvasia’, ‘Bianca’, and ‘Italia’, and the less susceptible
‘Alphonse Lavallée’ and ‘Ohanez’, in accordance with their behavior in the field. No statistically significant differences
were found in the virulence of E. necator isolates. 相似文献
12.
Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers 总被引:4,自引:0,他引:4
Y. Hayano-Saito T. Tsuji K. Fujii K. Saito M. Iwasaki A. Saito 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1044-1049
We used graphical genotyping and linkage analyses with molecular markers to determine the chromosomal location of the rice
stripe disease resistance gene, Stv-b
i
. The stripe resistance gene from the indica rice (Oryza sativa) cv ‘Modan’ was introgressed into several Japanese rice varieties. We found 4 RFLP markers in ‘Modan’, five susceptible parental
rice varieties (‘Norin No. 8’, ‘Sachihikari’, ‘Kanto No. 98’, ‘Hokuriku No.103’ and ‘Koganebare’) and four resistant progeny
varieties (‘St. No. 1’, ‘Aichi No. 6’, ‘Aoisora’ and ‘Asanohikari’). Graphical genotyping of the resistant progeny revealed
a chromosomal segment ascribable to ‘Modan’ and associated with stripe resistance. The chromosomal segment from ‘Modan’ was
located at 35.85 cM on chromosome 11. Linkage analysis using 120 F2 individuals from a cross between ‘Koshihikari’ (susceptible) and ‘Asanohikari’ (resistant) revealed another 8 RFLP markers
in the same chromosome. We performed a bioassay for rice stripe resistance in F3 lines of the F2 individuals using infective small brown planthoppers and identified an 1.8-cM segment harboring the rice stripe disease resistance
gene, Stv-b
i
, between XNpb220 and XNpb257/ XNpb254. Furthermore, Stv-b
i
was linked by 0.0 cM to a RFLP marker, ST10, which was developed on the basis of the results of RAPD analysis. These DNA
markers near the Stv-b
i
locus may be useful in marker-assisted selection and map-based cloning of the Stv-b
i
gene.
Received: 26 September 1997 / Accepted: 4 November 1997 相似文献
13.
W. E. Van de Weg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(8):1092-1096
Phytophthora fragariae var. fragariae is the causal agent of red stele (red core) root rot in strawberry (Fragaria spp.). The inheritance of resistance to one isolate of this fungus was studied in 12 segregating populations of F.×ananassa derived from crosses between four resistant cultivars (‘Climax’, ‘Redgauntlet’, ‘Siletz’, and ‘Sparkle’) and three susceptible
cultivars (‘Blakemore’, ‘Glasa’, and ‘Senga’ Sengana’). The analysis clearly supports the hypothesis of a single segregating
dominant resistance gene. It is proposed that this gene be designated Rpf2.
Received 12 November 1996 / Accepted: 22 November 1996 相似文献
14.
During vegetative period 2004–2005 powdery mildew (Erysiphe graminis DC. f. sp. hordei Em. Marchal) field resistance of spring barley cultivars was investigated at the Lithuanian Institute of Agriculture. The
spring barley genotypes tested were Lithuania-registered cultivars, cultivars from genetic resources collection, and the new
cultivars used for initial breeding. In total, 23 resistance genes were present in the 84 cultivars studied. Among mono-genes
only mlo and 1-B-53 showed very high resistance. Slight powdery mildew necroses (up to 3 scores) formed on cultivars possessing these genes.
The maximal powdery mildew (PM) severity reached a score of 8.5 and the area under disease progress curve (AUDPC) a value
of 1216.8. The cultivars ‘Primus’, ‘Astoria’, ‘Power’, ‘Harrington’ and ‘Scarlett’ were the most resistant among the non mlo cultivars. Severity of PM on ‘Primus’ reached a score of 3.5 (3.0 of PM necrosis) in average, the other cultivars were diseased
from 4.5 (3.0) to 5.0 (2.0). The AUDPC values for these cultivars except ‘Scarlett’ were the lowest (85.0–145.3) among the
other cultivars. The highest contrast in development of the other leaf diseases was between highly resistant and susceptible
to PM cultivar groups. The fast development of PM depressed development of the other diseases 4.7 times. 相似文献
15.
Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex
materials in a vacuum. It is widely applied to the discrimination of closely related microbial strains. Leaf samples from
eight cultivars (‘Apricot Delight’, ‘Cooler Grape’, ‘Cooler Peppermint’, ‘Equator Grape’, ‘Equator Rose’, ‘Equator White’,
‘Equator White Eye’, and ‘Little Bright Eye’) of Catharanthus roseus were subjected to PyMS for spectral fingerprinting. Discriminant analysis (DA) of PyMS data enabled us to assign these cultivars
to discrete clusters. A hierarchical dendrogram based on DA provided a possible relationship among them that was in general
agreement with a previously reported classification of the cultivars based on DNA fingerprints. Furthermore, those belonging
to the same ‘series’ were grouped into a single cluster, which previously could not be achieved through similar approaches
based on Fourier transform infrared spectroscopy or 1H NMR data. Overall results suggest that chemical differences (i.e., in pyrolysate composition) among cultivars, as detected
by mass spectrometry, reflect their genetic variation. 相似文献
16.
P. Barre M. Layssac A. D’Hont J. Louarn A. Charrier S. Hamon M. Noirot 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(2):301-305
F1 hybrids were obtained between two coffee species with the same chromosome number (2n=22) but with different nuclear DNA contents
[C. pseudozanguebariae (PSE) 2C=1.13 pg and C. liberica var ‘dewevrei’ (DEW) 2C=1.42 pg]. G2 hybrids were obtained by open-pollination of the F1 hybrids. Genomic in situ hybridisation (GISH) and flow cytometry were used on six F1 hybrids and seven G2 hybrids to determine their parental chromosomic contribution and their nuclear DNA content (qDNA), respectively.
GISH efficiently identified chromosomes from both species. F1 hybrids had a qDNA intermediate between that of the parental species and contained the expected 11 chromosomes from each
species. There was a linear relationship between the number of PSE chromosomes and the nuclear DNA content, which indicates
that flow cytometry can be used to give a rough estimate of the parental chromosomic contribution in G2 hybrids.
Received: 1 August 1997/Accepted: 25 August 1997 相似文献
17.
Experiments were conducted to quantify parasitism of Colorado potato beetle,Leptinotarsa decemlineata (Say), by the egg parasitoid,Edovum puttleri Grissell, on 3 different cultivars of eggplant,Solanum melongena L. Levels of parasitism were higher (P<0.05) on ‘Black Pride’ than on other cultivars. The percentage of egg masses that
were parasitized was 1.2-fold higher (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’. The number of eggs per
mass that were parasitized was 1.3- and 1.4- fold greater (P<0.05) on ‘Black Pride’ than on ‘Harris Special’ and ‘White’,
respectively. The percentage of eggs that were parasitized per mass and percentage of emerged adult parasitoids did not differ
(P>0.05) among cultivars; between 2.1- to 2.6- fold more females than males emerged from eggs on all cultivars during the
growing season.Edovum puttleri suppressed the 2nd generation ofL. decemlineata on ‘Black Pride’ and ‘Harris Special’, but did not suppress populations on ‘White’.
相似文献
18.
19.
G. P. Gill C. F. Harvey R. C. Gardner L. G. Fraser 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(3):439-445
Two sex-linked random amplified polymorphic DNA (RAPD) markers identified from Actinidia chinensis were converted into sequence-characterised amplified regions (SCARs) for the large-scale screening of Actinidia breeding populations. Initial SCAR primers converted one RAPD (SmX) into a dominant marker, but the other (SmY), which was
potentially more useful because of its linkage to the male determining ‘Y’ locus, failed to retain polymorphism. This difficulty
was overcome by cloning and sequencing the alternate ‘allele’ from female plants, and then designing ‘allele’-specific primers
that utilised nucleotide differences between the sexes. Using a quick squash-blot method of DNA extraction, the SCAR primers
were tested in 120 A. chinensis plants to determine their gender. The system is now in use for large-scale screening of seedling populations in the Actinidia breeding programme. The sex-linked SCAR primers also functioned with plants from some other geographically separate accessions
of A. chinensis and with plants in the closely related polyploid species A. deliciosa, but did not amplify a sex-linked band in more distantly related species of Actinidia.
Received: 27 December 1997 / Accepted: 5 March 1998 相似文献
20.
The purpose of this paper is to investigate the differential responses of flower opening to ethylene in two cut rose cultivars,
‘Samantha’, whose opening process is promoted, and ‘Kardinal’, whose opening process is inhibited by ethylene. Ethylene production
and 1-aminocyclopropane-1-carboxylate (ACC) synthase and oxidase activities were determined first. After ethylene treatment,
ethylene production, ACC synthase (ACS) and ACC oxidase (ACO) activities in petals increased and peaked at the earlier stage
(stage 3) in ‘Samantha’, and they were much more dramatically enhanced and peaked at the later stage (stage 4) in ‘Kardinal’
than control during vasing. cDNA fragments of three Rh-ACSs and one Rh-ACO genes were cloned and designated as Rh-ACS1, Rh-ACS2, Rh-ACS3 and Rh-ACO1 respectively. Northern blotting analysis revealed that, among three genes of ACS, ethylene-induced expression patterns of
Rh-ACS3 gene corresponded to ACS activity and ethylene production in both cultivars. A more dramatic accumulation of Rh-ACS3 mRNA was induced by ethylene in ‘Kardinal’ than that of ‘Samantha’. As an ethylene action inhibitor, STS at concentration
of 0.2 mmol/L generally inhibited the expression of Rh-ACSs and Rh-ACO in both cultivars, although it induced the expression of Rh-ACS3 transiently in ‘Kardinal’. Our results suggests that ‘Kardinal’ is more sensitive to ethylene than ‘Samantha’; and the changes
of Rh-ACS3 expression caused by ethylene might be related to the acceleration of flower opening in ‘Samantha’ and the inhibition in
‘Kardinal’. Additional results indicated that three Rh-ACSs genes were differentially associated with flower opening and senescence as well as wounding. 相似文献