鼠类对山杏(Prunus armeniaca)种子扩散及存活作用研究

虽然有关鼠类搬运森林种子的证据已很清楚,但这些被移走种子的存活情况却知之甚少.提出了一个新的标记和跟踪种子的方法--标签法,即将种子拴一带有编码的细长金属片,研究了北京东灵山地区山杏(Prunus armeniaca)种子的扩散距离和存活率.于1998年6月19～20日,7月3日和10月23日共在24个样点释放1440粒山杏种子.几乎所有释放的种子在10d内被鼠类取走.夏天释放的种子比秋天释放的种子消失的速度快.大多数种子的扩散距离在20m以内,小于鼠类的活动距离.鼠类吃掉种子的速度很快,但当种子变得稀少时,种子存活率有所提高.山杏种子6、7月份的每日存活率小于其它月份的每日存活率.     

鼠类对山杏种子存活和萌发的影响

山杏（Prunus armeniaca)是广布于北京山区阳坡的矮林或灌丛,易生存在土壤贫脊、干旱、或严重退化的山坡、裸地,对于保护生态环境,减少水土流失及促进山区经济发展均具有重要作用。有关鼠类在山杏更新中作用的研究较少,本研究主要是确定影响山杏更新的鼠类种类、鼠类对山杏种子的取食压力、人工埋藏种子及植被因素对种子存活和萌发的影响。于1997年10月3－4日,采用夹捕法同时调查取食花生和山杏种子的鼠类种类及种群密度。于1997年9月24－25日,通过在地表放置山桔种子,调查在鼠类取食下,地表种子消失的速度。于1997年10月20日,作种子埋藏实验,调查种子存活及萌发情况。于1997年10月26日,进行扣网实验,研究网内、外山杏种子存活及萌发情况。结果表明：大林姬鼠（Apodemus speciosus)、社鼠（Rattus confucianus),黑线姬鼠（Apodemus agrarius)为取食山杏种子的主要鼠种种类;与取食花生相比,社鼠更偏爱山杏种子,大林姬鼠对山杏种子喜食性略低,黑线姬鼠对二者喜食程度相近;山杏种子放置地表后1、10、20、30d的消失率分别为13.06%,64.46%,90.70%,96.69%;扣网实验说明,山杏在地表的萌发率极低,生长也慢;而埋入土层内可明显提高萌发率和生长率（若除去鼠类的取食,萌发率还要高）;人工埋藏实验表明,将山杏种子埋入土层5cm后,能够有效地减少鼠类对种子的取食;通过分析山杏种子萌发与植被的关系,发现山杏易在开阔、阳光充分的草丛中存活和生长,而不易在阴闭的灌丛下存活和生长。     

鼠类对山杏和辽东栎种子的贮藏

在北京市东灵山地区,选择灌丛生境,人工释放山杏和辽东栎种子,对两种种子在释放处被鼠类就地取食、存留动态、种子被搬运和贮藏的距离、位置、状态、微生境等进行了分析比较。采用活捕饲喂和咬痕比较的方法,对野外取食山杏和辽东栎种子的鼠类进行了鉴定。结果表明：1)取食山杏和辽东栎种子的鼠类主要是大林姬鼠和岩松鼠;2)山杏种子被鼠类搬运的平均距离大于辽东栎种子,两种种子大多数被搬运至21m之内,在9m之内最为集中;3)山杏种子的中位存留时间较辽东栎种子长;4)在释放处,鼠类对辽东栎种子的就地取食强度(54．83％)大于对山杏种子的取食(0．17％);5)在搬运之后,大部分山杏种子被埋藏起来,仅有3粒被取食;而辽东栎种子大部分被取食;6)鼠类趋向于把种子搬运到灌丛下方或灌丛边缘进行贮藏或取食。山杏种子在贮藏点的数量为1—3粒,而辽东栎种子在贮藏点的数量均为1粒。     

济源太行山区鼠类对三种林木种子的扩散和贮藏

对取食种子的动物而言,种子的选择、扩散以及随后的处理是一个复杂的过程.为了解济源太行山区鼠类对不同种林木种子的选择和扩散策略的差异,于2011年9月10日至11月8日,选取山杏、桃和栓皮栎3种林木种子,将种子标记后,释放于次生林中,以5d间隔,调查并记录种子命运.结果表明:(1)大林姬鼠和岩松鼠是该地区主要的种子取食者和扩散者;(2)3种种子的扩散速率明显不同,栓皮栎种子扩散速率最快(中位存留时间8.6d),其次为山杏种子(中位存留时间20.9 d),桃种子扩散速率最慢(中位存留时间37.5 d);(3)鼠类倾向于取食栓皮栎种子(55.0％),埋藏山杏种子(62.0％),但忽略桃种子(原地存留率99.0％);(4)88.6％的山杏和78.8％的栓皮栎种子被贮藏在灌丛下方、树干基部周围和石块旁边等生境中,而仅有4.3％的山杏和9.1％的栓皮栎种子被贮藏于裸地中;(5)鼠类将山杏种子搬运到更远(3.4±2.1 m,mean±SE,n=63)处贮藏;而栓皮栎种子的搬运距离则相对较近(2.5±2.4 m,n=57).结果显示:鼠类对不同种植物种子具有明显的取食、贮藏偏好和不同的贮藏策略.     

山杏的种子雨及鼠类的捕食作用

山杏(Prunus armeniaca)是一种灌木状小乔木,在北京山区较为贫瘠、干旱的阳坡地带广泛分布。于2002年7月和2003年7～9月,采用种子收集筐估计了山杏种子的产量、种子雨的动态变化过程;在2003年的研究中,把收集筐中所获得的种子转入扣网之中以排除鸟类的影响,并设置了地表对照样方,调查和比较了扣网内和对照样方中、扣网和对照样方之间山杏种子被取食、搬离的动态变化及其差异性。研究表明,山杏种子的下落高峰发生于7月下旬至8月上旬,败育种子的下落早于完好种子且数量极少;在扣网内和对照样方中,被取食和被搬运的种子数量之间存在显著性差异,在扣网和对照样方之间．被取食和被搬运的种子数量的差异均未达到显著性水平;鼠类是种子雨期间取食山杏种子的主要动物．鸟类的作用可以忽略。     

外果皮厚度和种子大小对五种栎属橡子扩散的影响(英文)

动物对种子的扩散和贮藏是一个复杂的生态学过程,常常受到种子特征的影响。有关种子特征如何影响动物对种子扩散,许多研究结果并非完全一致。我们于2009年9月在黑龙江东方红林场野外和围栏内释放五种栎属橡子(Quercus mongolica,Q.serrata var.brevipetiolata,Q.aliena,Q.variabilis和Q.liaotungensis),研究种子特征对鼠类(Apodemus peninsulae,Clethrionomys rufocanus和Tamias sibiricus)扩散和埋藏橡子的影响。野外释放结果表明:橡子大小和外果皮厚度显著影响鼠类对橡子的扩散和埋藏。鼠类偏向扩散和埋藏种皮厚的大橡子,种皮薄的小橡子则多被原地取食。种皮厚的大橡子扩散距离显著高于种皮薄的小橡子。然而,只有外果皮的厚度显著影响围栏内花鼠对橡子的扩散和埋藏,橡子大小并非主要的影响因素。种子特征影响种子扩散的效应可能在种群和群落水平上存在差异。     

种子产量对鼠类扩散栓皮栎坚果的影响

为了解林木种子产量对鼠类种子扩散行为的影响,于2008年和2009年,在国有济源市愚公林场调查了栓皮栎的种子雨;在每年的种子雨结束后,选择次生林生境,研究了鼠类对人工释放栓皮栎坚果的扩散,旨在探讨种子产量与种子扩散间的关系。结果表明:1)取食栓皮栎坚果的鼠类主要有大林姬鼠、社鼠和岩松鼠,2008年的鼠类捕获率(2%)低于2009年(10%),其差异并未达到显著性水平;2)栓皮栎的种子雨构成和产量存在年际差异,2008年以完好种子为主且产量高于2009年,而2009年以败育种子为主;3)2008年人工释放栓皮栎坚果的中位存留时间显著高于2009年;4)2008年鼠类对栓皮栎坚果的平均扩散距离显著低于2009年,且两年的搬运距离几乎都集中在9m以内;5)2008年鼠类对栓皮栎坚果的埋藏比例显著高于2009年。结果提示,在种子高产年份,可能有较多的种子逃脱动物的取食,从而增加种子萌发和幼苗建成的机会,最终促进植物的更新。     

森林鼠类对秦岭南坡3种壳斗科植物种子扩散的影响

种子作为森林鼠类的主要食物来源,它们的扩散和更新很大程度上依赖于鼠类的传播。在鼠类扩散种子的过程中,种子特征和食物相对丰富度是影响鼠类对种子进行何种选择策略的重要因素。2011—2012年的8—12月,采用塑料片标记法在秦岭南坡的佛坪国家级自然保护区内调查了森林鼠类对同域分布的3种壳斗科植物(锐齿槲栎Quercus aliena var.acuteserrata、栓皮栎Q.variabilis和短柄枹栎Q.serrata var.brevipetiolata)种子的扩散差异。结果表明:(1)鼠类倾向于贮藏营养价值较大的栓皮栎种子,并且其贮藏距离也最远(2011:1.52 m,2012:4.03 m),3种种子在食物相对丰富度较低年份被贮藏的距离均较远。(2)在食物相对丰富度较高的年份(2011),种子的消耗速率较慢,在种子释放10 d后种子释放点仍有67.33%的种子,贮藏量较高,至实验结束仍有29.67%的种子被贮藏。在食物相对丰富度较低的年份(2012),种子消耗速率较快,在种子释放后10 d内所有种子均被取食或搬离种子释放点,贮藏量较低,至实验结束仅有3.83%的种子仍被贮藏。(3)虽然栓皮栎种子的贮藏量最大,被贮藏后的存留量也最大,但其在实验地的分布却较小,说明种子扩散仅是植物分布与存活的第一步。以上结果表明,鼠类倾向于贮藏营养价值高的种子。在食物相对丰富度较高的年份会更多的贮藏种子,但种子被贮藏的距离较近,在食物相对丰富度较低的年份会更多的取食种子。     

胡桃楸种子大小对鼠类分散贮藏行为的影响——基于无线电标记技术

种子大小是影响鼠类对种子扩散和分散贮藏的一个重要因素,但有关种子大小对分散贮藏影响的研究结果尚存在一定争议。2012年9月,在小兴安岭南麓带岭区东方红林场利用微型无线电标记技术研究鼠类对大、小胡桃楸种子的多次分散贮藏,进一步探讨种子大小对种子扩散的影响。结果表明:1)胡桃楸种子大小并不影响鼠类对坚果的选择及分散贮藏偏好。胡桃楸大、小种子被扩散的比例分别为100%和95.6%,鼠类对两类种子的埋藏率也十分接近(χ2=0.045,df=1,P=0.831);2)鼠类对胡桃楸大、小种子的扩散次数无显著差异(F=2.710,df=1,P=0.103),大种子被鼠类扩散的平均次数为1.67±0.10,小种子为1.91±0.10。3)胡桃楸大种子的初次、次级扩散距离均显著高于小种子,种子大小也显著影响扩散的净距离和总距离,大种子比小种子扩散的较远。基于无线电标记技术,研究结果准确评价了种子大小对胡桃楸种子扩散距离的影响,进一步支持了大种子扩散较远的假说。     

季节变化对锐齿栎种子扩散的影响

季节变化是影响鼠类扩散植物种子的一个主要环境因素.2008年9-11月(秋季)和2009年3-4月(春季)分别在秦岭南坡的佛坪国家级自然保护区内调查了森林鼠类对锐齿栎种子的扩散,并与种子大小年假说的预测进行了比较分析.结果表明:春季锐齿栎种子的扩散速度比秋季快,平均扩散距离(取食距离和贮藏距离)也比秋季远,该结果与种子...     

含水量对种子贮藏寿命的影响

建立以收集种子为主体的基因库乃是当今保护植物种质资源最为普遍且可靠易行的方式,在世界库存约 61 0 0 0 0 0份种质资源中,近 90 %是以种子形式保存于约 1 30 0个基因库中。低温贮藏仍是目前基因库中种子种质保存的主要方法。种子含水量和贮藏温度是影响种子在贮藏期间生活力和活力保持的关键因素。传统的经验认为控制温度比控制水分来得安全有效,因而趋向于向低温或超低温的贮藏方向发展。国际植物遗传资源研究所（ＩＰＧＲＩ）曾推荐 5%～ 6%的含水量和 - 1 8～- 2 0℃低温作为各国长期保存种子的理想条件。目前,世界各国都把更多的…     

Seed survival of the tropical tree Cryptocarya aschersoniana (Lauraceae): Consequences of habitat disturbance

Seed survival in soil could be strongly influenced by habitat characteristics, but little is known about the behaviour of seeds sensitive to desiccation in seed banks installed in natural or disturbed habitats. Cryptocarya aschersoniana seeds disperse at the end of the rainy season but do not germinate immediately; thus, they may form seed banks in soil. This study evaluated the behaviour of C. aschersoniana seed banks induced in the natural environment of the species and in a disturbed area. Recently harvested C. aschersoniana germination units were characterized according to their water content, germination and viability. In 2011 and 2012, seed banks were established by burying samples of seeds in the understory of a semi‐deciduous forest. In 2012, samples were also buried in a disturbed area. The seed banks were sampled at certain time intervals, and the samples were characterized as described above. Precipitation and air temperature data were collected. As a result, seeds in the seed bank established in the natural environment form a transient seed bank and showed the same behaviour in both years studied. A germination peak was observed starting 210 days after burial (coinciding with the onset of the rainy season) and reached germination percentages higher than 80% at the end of the experiment for both years. Seed mortality did not exceeded 28% in the natural environment. However, in the disturbed environment, the seeds lost their viability more rapidly, with 90% of the seeds becoming unviable 240 days after burial. Germinated seeds in the disturbed environment (maximum 21%) were not able to establish seedlings. These results underscore the importance of maintaining a natural, undisturbed forest for the conservation of this species.     

不同种子预处理对10种沙拐枣植物萌发的影响

为了确定沙拐枣植物种子的萌发特性及最优播前预处理方法,在实验室条件下,对10种沙拐枣植物的种子进行了磨砺、硫酸和热水浸泡、冷藏、种子浸出液处理,然后进行发芽实验研究。萌发实验的结果表明,10种沙拐枣植物对于不同的种子预处理,均表现出相似的萌发反应。磨砺、硫酸浸泡和冷藏处理对种子萌发有明显地促进作用。与对照相比,种子浸出液处理对种子的发芽率、发芽速度均具有明显地抑制作用,并能增强种子的休眠。冷藏处理具有打破有活力的种子休眠、促进种子萌发的作用,但它与热水浸泡处理一样,对有活力种子表现出一定的致死作用。沙拐枣植物的萌发模式在不同种子预处理问均表现出明显的差异性。机械磨擦和硫酸处理能够促进种子的萌发率及发芽势。泡果沙拐枣(Callingonum junceum)在本项实验中表现出很强的萌发能力。     

The ecophysiology of seed persistence: a mechanistic view of the journey to germination or demise

Seed persistence is the survival of seeds in the environment once they have reached maturity. Seed persistence allows a species, population or genotype to survive long after the death of parent plants, thus distributing genetic diversity through time. The ability to predict seed persistence accurately is critical to inform long‐term weed management and flora rehabilitation programs, as well as to allow a greater understanding of plant community dynamics. Indeed, each of the 420000 seed‐bearing plant species has a unique set of seed characteristics that determine its propensity to develop a persistent soil seed bank. The duration of seed persistence varies among species and populations, and depends on the physical and physiological characteristics of seeds and how they are affected by the biotic and abiotic environment. An integrated understanding of the ecophysiological mechanisms of seed persistence is essential if we are to improve our ability to predict how long seeds can survive in soils, both now and under future climatic conditions. In this review we present an holistic overview of the seed, species, climate, soil, and other site factors that contribute mechanistically to seed persistence, incorporating physiological, biochemical and ecological perspectives. We focus on current knowledge of the seed and species traits that influence seed longevity under ex situ controlled storage conditions, and explore how this inherent longevity is moderated by changeable biotic and abiotic conditions in situ, both before and after seeds are dispersed. We argue that the persistence of a given seed population in any environment depends on its resistance to exiting the seed bank via germination or death, and on its exposure to environmental conditions that are conducive to those fates. By synthesising knowledge of how the environment affects seeds to determine when and how they leave the soil seed bank into a resistance–exposure model, we provide a new framework for developing experimental and modelling approaches to predict how long seeds will persist in a range of environments.     

壳斗科三种植物种子大小对昆虫寄生及种子存活率的影响

种子内的寄生昆虫可以严重影响种子的发育、损害种子活力。种子足余策略理论认为大种子有利于抵御和适应昆虫寄生取食,但动物最优觅食理论推测,大种子更易遭受昆虫寄生。为对这两种对立观点进行验证,本实验以青冈、苦槠和麻栎各2个种群的种子为材料,对昆虫寄生与完好种子间的体积和萌发率进行比较,并对寄生种子萌发率与种子体积的关系进行了分析。结果显示:(1)在6个种群的种子中,只有松阳麻栎和青冈种群的寄生种子体积大于完好种子,其余4个种群的寄生种子体积小于完好种子,但这种差异不显著;(2)所有寄生种子的整体萌发率(18%)显著低于完好种子(45.66%)(P<0.001),在不同种群内,寄生种子的萌发率也分别显著低于完好种子。(3)比较同种植物体积差异显著的寄生种子的萌发率发现,大种子总比小种子具有更高的萌发率,但差异不显著;在不同植物的寄生种子间比较时,体积最大的麻栎种子萌发率显著高于体积较小的青冈和苦槠种子。研究结果表明,象虫在种子上产卵时对大种子没有选择偏好,在昆虫寄生取食严重损害种子活力的压力下,大种子比小种子具有更强的耐受力。     

A comparative study of seed yield parameters in Arabidopsis thaliana mutants and transgenics

Because seed yield is the major factor determining the commercial success of grain crop cultivars, there is a large interest to obtain more understanding of the genetic factors underlying this trait. Despite many studies, mainly in the model plant Arabidopsis thaliana, have reported transgenes and mutants with effects on seed number and/or seed size, knowledge about seed yield parameters remains fragmented. This study investigated the effect of 46 genes, either in gain- and/or loss-of-function situations, with a total of 64 Arabidopsis lines being examined for seed phenotypes such as seed size, seed number per silique, number of inflorescences, number of branches on the main inflorescence and number of siliques. Sixteen of the 46 genes, examined in 14 Arabidopsis lines, were reported earlier to directly affect in seed size and/or seed number or to indirectly affect seed yield by their involvement in biomass production. Other genes involved in vegetative growth, flower or inflorescence development or cell division were hypothesized to potentially affect the final seed size and seed number. Analysis of this comprehensive data set shows that of the 14 lines previously described to be affected in seed size or seed number, only nine showed a comparable effect. Overall, this study provides the community with a useful resource for identifying genes with effects on seed yield and candidate genes underlying seed QTL. In addition, this study highlights the need for more thorough analysis of genes affecting seed yield.     

Lack of adequate seed supply is a major bottleneck for effective ecosystem restoration in Chile: friendly amendment to Bannister et al. (2018)

We argue that the need for a quality seed supply chain is a major bottleneck for the restoration of Chile's native ecosystems, thus supplementing the list of bottlenecks proposed by Bannister et al. in 2018. Specifically, there is a need for defining seed transfer zones, developing standards and capacities for properly collecting and storing seeds, reducing information gaps on seed physiology and longevity, and implementing an efficient seed supply chain with certification of seed origin and quality. Without such capacities, countries are unlikely to meet their restoration commitments. Although we focus on bottlenecks in Chile, the issues we raise are relevant to other countries and thus the global agenda for ecological restoration.     

Seed dynamics of resprouting shrubs in grassy woodlands: Seed rain,predators and seed loss constrain recruitment potential

Abstract Measuring the fate of seeds between seed production and seedling establishment is critical in understanding mechanisms of recruitment limitation of plants. We examined seed fates to better understand the recruitment dynamics of four resprouting shrubs from two families (Fabaceae and Epacridaceae) in temperate grassy woodlands. We tested whether: (i) pre‐dispersal seed predation affected seed rain; (ii) post‐dispersal seed predation limited seed bank accumulation; (iii) the size of the seed bank was related to seed size; and (iv) viable seeds accumulated in the soil after seed rain. There was a distinct difference in seed production per plant between plant families with the legumes producing significantly more seeds per individual than the epacrids. Seed viability ranged from 43% to 81% and all viable had seed or fruit coat dormancy broken by heat or scarification. Pre‐dispersal predation by Lepidopteran larvae removed a large proportion of seed from the legume seed rain but not the epacrids. Four species of ants (Notoncus ectatomoides, Pheidole sp., Rhytidoponera tasmaniensis and Iridomyrmex purpureus) were major post‐dispersal seed removers. Overall, a greater percentage of Hardenbergia (38%) and Pultenaea (59%) seeds were removed than the fleshy fruits of Lissanthe (14%) or Melichrus (0%). Seed bank sizes were small (<15 seeds m^?2) relative to the seed rain and no significant accumulation of seed in the soil was detected. Lack of accumulation was attributed to seed predation as seed decay was considered unlikely and no seed germination was observed in our study sites. Our study suggests that seed predation is a key factor contributing to seed‐limited recruitment in grassy woodland shrubs by reducing the number of seeds stored in the soil.     

Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions

Background and Aims

Despite differences in physiology between dry and relative moist seeds, seed ageing tests most often use a temperature and seed moisture level that are higher than during dry storage used in commercial practice and gene banks. This study aimed to test whether seed ageing under dry conditions can be accelerated by storing under high-pressure oxygen.

Methods

Dry barley (Hordeum vulgare), cabbage (Brassica oleracea), lettuce (Lactuca sativa) and soybean (Glycine max) seeds were stored between 2 and 7 weeks in steel tanks under 18 MPa partial pressure of oxygen. Storage under high-pressure nitrogen gas or under ambient air pressure served as controls. The method was compared with storage at 45 °C after equilibration at 85 % relative humidity and long-term storage at the laboratory bench. Germination behaviour, seedling morphology and tocopherol levels were assessed.

Key Results

The ageing of the dry seeds was indeed accelerated by storing under high-pressure oxygen. The morphological ageing symptoms of the stored seeds resembled those observed after ageing under long-term dry storage conditions. Barley appeared more tolerant of this storage treatment compared with lettuce and soybean. Less-mature harvested cabbage seeds were more sensitive, as was the case for primed compared with non-primed lettuce seeds. Under high-pressure oxygen storage the tocopherol levels of dry seeds decreased, in a linear way with the decline in seed germination, but remained unchanged in seeds deteriorated during storage at 45 °C after equilibration at 85 % RH.

Conclusions

Seed storage under high-pressure oxygen offers a novel and relatively fast method to study the physiology and biochemistry of seed ageing at different seed moisture levels and temperatures, including those that are representative of the dry storage conditions as used in gene banks and commercial practice.