共查询到13条相似文献,搜索用时 125 毫秒
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本文应用悬滴培养、燕麦-琼脂培养等方法及显微成像技术对绒泡菌科Physaraceae 4种黏菌(黄头绒泡菌Physarum flavicomum、淡黄绒泡菌Physarum melleum、垂头绒泡菌Physarum album、针箍菌Physarella oblonga)的个体发育特征进行比较研究,首次实现了垂头绒泡菌在实验室条件下的完整生活循环。4种黏菌孢子萌发均为V-型开裂,但萌发时间有所不同,黄头绒泡菌萌发所需时间最短,仅需5h,而针箍菌萌发所需时间最长,需要2d;针箍菌和淡黄绒泡菌形成的黏变形体均可转变为游动胞,而相同条件下黄头绒泡菌和垂头绒泡菌并未有游动胞形成;4种黏菌原生质团的生长速率不同,在光照刺激下针箍菌、淡黄绒泡菌、黄头绒泡菌和垂头绒泡菌的原生质团分别经过2-3d、2-3d、7d和13d,发育成子实体;完成孢子到孢子的整个生活史,针箍菌和淡黄绒泡菌需要25-30d,黄头绒泡菌和垂头绒泡菌需要40-45d。 相似文献
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Myxomycetes (slime molds) are unique eukaryotic microorganisms with both characteristics of fungi and amoebae. Artificial cultures grown under controlled conditions were used to study the life cycle and morphogenesis. Physarum pusillum was collected from the field. Spores were inoculated and cultured with the hanging drop method. The complete life cycle was observed from spore to spore on agar without adding any solid nutrients or bacteria as food. Life cycle morphological characteristics were described for spore germination, myxamoebae, zygote, plasmodium and sporangia formation. 相似文献
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The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms.
Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications
was demonstrated. Details of spore-to-spore development are described and illustrated. 相似文献
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N. B. Matveeva V. A. Teplov A. R. Nezvetsky T. G. Orlova S. I. Beylina 《Biophysics》2012,57(5):644-650
Possible involvement of autocrine factors into the control of motile behavior via a receptor-mediated mechanism was investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the auto-oscillatory mode of motility. Cyclic adenosine monophosphate (cAMP) and extracellular cAMP-specific phosphodiesterase, its involvement into the control of plasmodium motile behavior was proved by action of its strong inhibitor, were regarded as putative autocrine factors. It was shown that the plasmodium secreted cAMP. When it was introduced into agar support, 0.1–1 mM cAMP induced a delay of the plasmodium spreading and its transition to migration. When locally applied, cAMP at the same concentrations induced the typical for attractant action increase in oscillation frequency and the decrease of ectoplasm elasticity. The ability to exhibit positive chemotaxis in cAMP gradient and the dependence of its realization were shown to depend on the plasmodium state. Chemotaxis test specimens obtained from the migrating plasmodium, unlike those obtained from growing culture, generate alternative fronts which compete effectively with fronts oriented towards the attractant increment. The results obtained support our supposition stated earlier that advance of the Physarum polycephalum plasmodium leading edge is determined by local extracellular cAMP gradients arising from a time delay between secretion and hydrolysis of the nucleotide. 相似文献