贵州东部地区茶园土壤甲螨种类调查研究（甲螨亚目）

报道了贵州东部地区茶园土壤甲螨104种,分属42科.以奥甲螨科、单翼甲螨科、盲甲螨科、菌甲螨科、上罗甲螨科等为茶园土壤甲螨优势类群,其中新小奥甲螨OppieUa nova,卵角单翼甲螨Rostrozetes ovulum,棒菌甲螨Scheloribates latipes,长刺表珠甲螨Epidameus longispinosus,异毛裂甲螨 Meristacarus heterotrichus,棘洼甲螨Camisia spinifer,缓三盲甲螨Trimalconothrus tardus,双毛角甲螨Ceratoppia bipillis,南方珠足甲螨Belba sasakawail,叶小盾珠甲螨Sactobelbella frondosa,尼兰奥甲螨Oppia neerlandia等为茶园土壤甲螨类动物常见种类.     

洼甲螨科的分类研究进展(蜱螨亚纲:甲螨目:懒甲螨总科)

洼甲螨科隶属于蜱螨亚纲甲螨目懒甲螨总科,是低等甲螨中进化相对较高级,介于低等甲螨和高等甲螨之间的过渡类群,广泛分布于世界各大动物地理区,目前全世界有3属5亚属79种5亚种,国内现知2属16种.综述了洼甲螨科该科的分类沿革及分类研究进展;概述了洼甲螨科各属(亚属)的地理分布,指出该科目前所知分布较多的是古北界、新北界、东洋界;对中国洼甲螨科已知种进行了区系总结,目前青藏区和蒙新区各仅知1种,主要原因是缺乏深入研究;编制了洼甲螨科3个属及5个亚属和中国已知2属16种的分类检索表.     

中国贵州懒甲螨属(蜱螨亚纲,甲螨目,懒甲螨科)及一新种记述

记述中国贵州懒甲螨科Nothridae懒甲螨属Nothrus 5种。编制了分种检索表,其中包括1新种贵阳懒甲螨Nothrus guiyangensis sp.nov.。新种与山地懒甲螨N.monticola Hammer,1961和湿地懒甲螨N.meakanensis近似,与山地懒甲螨的区别在于:新种后背板毛c1长度是c2的1.3倍,后背板刚毛h1不着生于瘤突上,梁毛的形状和后背板毛的形状相似,都是叶状。而后者毛c2长度是c1的一半,毛h1着生于瘤突上,叶毛呈刚毛状,而后背板毛呈叶状。与湿地懒甲螨的区别在于,h1不达于后背板的末端,吻毛无透明的角质鞘,且长度只有两吻毛着生基部间距的1/3,而湿地懒甲螨的h1超过后背板的末缘,吻毛具有透明的角质鞘,且长度只比两吻毛着生基部间距略短。所有标本均保存于贵州大学昆虫研究所标本馆。     

中国暗色甲螨属(甲螨亚目,尖棱甲螨科)二新纪录种记述(英文)

记述了采自黑龙江省和辽宁省的尖棱甲螨科中国2新纪录种:梭暗色甲螨Fuscozetes fuscipes Koch,1844和塔暗色甲螨Fuscozetes tatricus Seniczak,1993,并对这2个种进行了详细的绘图和描述,同时编制了该属中国已知种检索表。     

贵州梵净山自然保护区土壤甲螨群落的季节动态

2001年7月至2002年4月在不同的季节,对梵净山自然保护区属级水平上土壤甲螨结构的季节变化进行了调查.结果显示,土壤甲螨多度的四季变化为春季＞冬季＞秋季＞夏季.土壤甲螨多样性的四季变化为秋季＞夏季＞冬季＞春季.不同的季节土壤甲螨群落组成不同,但小奥甲螨属、小盾珠甲螨属、木单翼甲螨属3个属在每个季节均为绝对优势属.土壤甲螨主要分布在表层,但从夏季到冬季,土壤甲螨从表层向深层迁徙.     

中国西藏甲螨新种和新纪录种记述(蜱螨亚纲:甲螨亚目)

报道西藏甲螨4新种和6新纪录种,标本采于西藏东南色季拉山,海拔3640－4800m,新种为：粒小隅甲螨Microtegeus granulatus Wang et Shen,sp.nov.,西藏真翅背甲螨Eupterotegaeus xizangensis Wang et Solhoy,sp.nov.,头新裸甲螨Neogymnobates capitatus Wang et Solhoy,sp.nov.,山溯甲螨Birsteinius monticolus Wang et Shen,sp.nov.,新纪录种为：纽氏平懒甲螨 Platynothrus nomatai Aoki,塞氏矮汉甲螨Nanhermannia sellnicki Forsslund,杜氏小赫甲螨Hermanniella dubinini Sitnikova,多齿刀肋甲螨Cultroribula dentata Willmann,曲波甲螨Unduloribates undulatus(Berlese),毛暗色甲螨Fuscozetes setosus(C.L.koch)。本文报道的波甲螨属,溯甲螨属,新裸甲螨属和暗色甲螨属为中国首次发现。     

中国南方珠甲螨属种类纪要（蜱螨亚纲：甲螨亚目：珠甲螨科）

本文记述我国南方土壤甲螨珠甲螨属Ｄａｍａｅｕｓ Ｋｏｃｈ种类共９种,其中包括５新种：矩刺珠甲螨Ｄ．ｓｐｉｎｉｇｅｒ ｓｐ．ｎｏｖ．,短毛洙甲螨Ｄ．ｂｒｅｖｉｓｅｔｕｓ ｓｐ．ｎｏｖ．,姚氏珠甲螨Ｄ．ｙａｏｉ ｓｐ．ｎｏｖ．,巨突珠甲螨Ｄ．ｅｘｓｅｒｔｕｓ ｓｐ．ｎｏｖ．,鞭毛珠甲螨Ｄ．ｆｌａｇｅｌｌａｔｕｓ ｓｐ．ｎｏｖ．;１个中国新纪录盔珠甲螨Ｄ．ａｒｍａｔｕｓ（Ａｏｋｉ）;２项新组合：将ａｒｍ     

中国珠足甲螨属一新种及珠足甲螨属和孔珠足甲螨属的新纪录（蜱螨亚纲：甲螨亚目：珠甲螨科）

本文记述中国珠足甲螨属ＢｅｌｂａＶｏｎＨｅｙｄｅｎ的一新种角珠足甲螨Ｂ．ｃｏｒｎｕｔａｓｐ．ｎｏｖ,珠足甲螨属和孔珠足甲螨属中国６个新纪录种：南方珠足甲螨Ｂ．ａｓａｋａｗａｉＥｎａｍｉ、俄罗斯珠足甲螨Ｂ．Ｂｕｌａｎｏｖａ－Ｚａｃｈｖａｔｋｉｎａ,疣珠足甲螨Ｂ．ｖｅｒｒｕｃｏｓａＢ．－Ｚ．,塞氏珠足甲螨Ｂ．ｓｅｌｌｎｉｃｋｉＢ．－Ｚ．,棒珠足甲螨Ｂ．ｃｏｒｙｎｏｐｕｓ（Ｈｅｒｍａｎｎ）和刺孔珠足甲螨Ｐｏｒｏｂｅｌｂａｓｐｉｎｏｓａ（Ｓｅｌｌｎｉｃｋ）,并对上述部分种类作了补充描述。     

中国丽甲螨属新种和新纪录种(蜱螨亚纲:甲螨亚目:丽甲蟎科)

本文记述了丽甲螨科Liacaridae丽甲螨属LiacarusMichael,1898的四新种:多叉丽甲螨L.polychothomussp.nov.,宽突丽甲螨L.latiusculussp.nov.,峨眉丽甲螨L.emeiensissp.nov.,北丽甲螨L.borealissp.nov.,并报道一个中国新纪录种直角丽甲螨L.orthogoniosAoki,1959。本文对新种进行了形态特征描述并与近似种作了比较鉴别。     

我国裂头甲螨属2新种和1新纪录种记述（蜱螨亚纲：甲螨亚目：耳头甲螨科）

报道了发现于我国的裂头甲螨属Fissicepheus 2新种:中国裂头甲螨,新种F.chinensis sp.nov.,喙裂头甲螨,新种F.ornithorrhynchus sp.nov.,描述了它们的形态特征并与近似种作了比较鉴别。此外,还记述了我国1新纪录种——温和裂头甲螨F.milis Aoki,1970.并对于我国产4种裂头甲螨进行了种的检索。     

Truffle trouble: what happened to the Tuberales?

An overview of truffles (now considered to belong in the Pezizales, but formerly treated in the Tuberales) is presented, including a discussion on morphological and biological traits characterizing this form group. Accepted genera are listed and discussed according to a system based on molecular results combined with morphological characters. Phylogenetic analyses of LSU rDNA sequences from 55 hypogeous and 139 epigeous taxa of Pezizales were performed to examine their relationships. Parsimony, ML, and Bayesian analyses of these sequences indicate that the truffles studied represent at least 15 independent lineages within the Pezizales. Sequences from hypogeous representatives referred to the following families and genera were analysed: Discinaceae–Morchellaceae (Fischerula, Hydnotrya, Leucangium), Helvellaceae (Balsamia and Barssia), Pezizaceae (Amylascus, Cazia, Eremiomyces, Hydnotryopsis, Kaliharituber, Mattirolomyces, Pachyphloeus, Peziza, Ruhlandiella, Stephensia, Terfezia, and Tirmania), Pyronemataceae (Genea, Geopora, Paurocotylis, and Stephensia) and Tuberaceae (Choiromyces, Dingleya, Labyrinthomyces, Reddellomyces, and Tuber). The different types of hypogeous ascomata were found within most major evolutionary lines often nesting close to apothecial species. Although the Pezizaceae traditionally have been defined mainly on the presence of amyloid reactions of the ascus wall several truffles appear to have lost this character. The value of the number of nuclei in mature ascospores as a delimiting family character is evaluated and found to be more variable than generally assumed.     

Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology

The family Sordariaceae incorporates a number of fungi that are excellent model organisms for various biological, biochemical, ecological, genetic and evolutionary studies. To determine the evolutionary relationships within this group and their respective phylogenetic placements, multiple-gene sequences (partial nuclear 28S ribosomal DNA, nuclear ITS ribosomal DNA and partial nuclear β-tubulin) were analysed using maximum parsimony and Bayesian analyses. Analyses of different gene datasets were performed individually and then combined to generate phylogenies. We report that Sordariaceae, with the exclusion Apodus and Diplogelasinospora, is a monophyletic group. Apodus and Diplogelasinospora are related to Lasiosphaeriaceae. Multiple gene analyses suggest that the spore sheath is not a phylogenetically significant character to segregate Asordaria from Sordaria. Smooth-spored Sordaria species (including so-called Asordaria species) constitute a natural group. Asordaria is therefore congeneric with Sordaria. Anixiella species nested among Gelasinospora species, providing further evidence that non-ostiolate ascomata have evolved from ostiolate ascomata on several independent occasions. This study agrees with previous studies that show heterothallic Neurospora species to be monophyletic, but that homothallic ones may have a multiple origins. Although Gelasinospora and Neurospora are closely related and not resolved as monophyletic groups, there is insufficient evidence to place currently accepted Gelasinospora and Neurospora species into the same genus.     

Genomic characterization of self-incompatibility ribonucleases (S-RNases) in European pear cultivars and development of PCR detection for 20 alleles

Sexual self-incompatibility in European pear (Pyrus communis L.) is controlled by a single locus (S-locus) encoding a polymorphic stylar ribonuclease (S-RNase) that is responsible for the female function in pollen–pistil recognition. In this study, genomic DNA sequences corresponding to five new S-RNase alleles (named S ₂₀ , S ₂₁ , S ₂₂ , S ₂₃ , and S ₂₄ ) and to S _m were characterized in European pear cultivars. Re-sequencing S _q from ‘General Le Clerc’ showed this S-RNase to encode the same protein as S ₁₂ . Based on these findings, a polymerase chain reaction (PCR)-based method was developed for the molecular typing of cultivars bearing 20 S-RNases (S ₁ –S ₁₄ , S _m , and S ₂₀ –S ₂₄ ) using consensus and allele-specific primers. Genomic PCR with consensus primers amplified product sizes characteristic of the S-RNases S ₁ , S ₂ , S ₄ , S ₁₀ , S ₁₃ , and S ₂₀ . However, the allele groups S ₃ /S ₁₂ , S ₆ /S ₈ /S ₁₁ /S ₂₂ and S ₅ /S ₇ /S ₉ /S ₁₄ /S _m /S ₂₁ /S ₂₃ /S ₂₄ amplified PCR products of similar size. To discriminate between alleles within these groups, primers to specifically amplify each S-RNase were developed. Application of this approach in 19 cultivars with published S-alleles allowed re-evaluation of one of the alleles of ‘Passe Crassane,’ ‘Conference,’ and ‘Condo.’ Finally, this method was used to assign S-genotypes to 37 cultivars. Test crosses confirmed molecular results. Electronic supplementary material  The online version of this article (doi:) contains supplementary material, which is available to authorized users.     

Genetic analysis of constitutive stable DNA replication in rnh mutants of Escherichia coli K12

Summary Escherichia coli rnh mutants deficient in ribonuclease H (RNase H) are capable of DNA replication in the absence of protein synthesis. This constitutive stable DNA replication (SDR) is dependent upon the recA ⁺ gene product. The requirement of SDR for recA ⁺ can be suppressed by rin mutations (for recA⁺-independent), or by lexA(Def) mutations which inactivate the LexA repressor. Thus, there are at least three genetically distinct types of SDR in rnh mutants: recA ⁺-dependent SDR seen in rnh ^- rin⁺ lexA⁺ strains, recA ⁺-independent in rnh ^- rin^- lexA⁺, and recA ⁺-independent in rnh ^- rin⁺ lexA(Def). The expression of SDR in rin ^- and lexA(Def) mutants demonstrated a requirement for RNA synthesis and for the absence of RNase H. The suppression of the recA ⁺ requirement by rin mutations was shown to depend on some new function of the recF ⁺ gene product. In contrast, the suppression by lexA-(Def) mutations was not dependent on recF ⁺. The lexA3 mutation inhibited recA ⁺-dependent SDR via reducing the amount of recA ⁺ activity available, and was suppressed by the recAo254 mutation. The SDR in rnh ^- rin^- cells was also inhibited by the lexA3 mutation, but the inhibition was not reversed by the recAo254 mutation, indicating a requirement for some other lexA ⁺-regulated gene product in the recA ⁺-independent SDR process. A model is presented for the regulation of the expression of these three types of SDR by the products of the lexA ⁺, rin⁺ and recF ⁺ genes.     

苏云金芽胞杆菌幕虫亚种晶胞粘连现象与晶体蛋白基因cry26Aa所在质粒有关

苏云金芽胞杆菌幕虫亚种T02菌株的伴胞晶体在芽胞外壁内侧形成,呈现晶胞粘连的现象。在此菌株中克隆了cry26Aa和cry28Aa两个基因,并对晶胞粘连现象与质粒的相关性做了系统研究。通过消除幕虫亚种T02菌株的质粒,得到了仅消除cry26Aa所在质粒的菌株BMB1151和无质粒的菌株BMB1152。通过穿梭载体将cry26Aa和cry28Aa两个基因分别和同时转化无质粒突变株BMB1152并表达,形成的晶体与芽胞独立存在不能粘连,表明在幕虫亚种染色体背景下仅仅cry的表达不能形成晶胞粘连现象,从而推断晶胞粘连现象可能与幕虫亚种两个基因所在的质粒有关;进一步的研究发现将cry26Aa在仅消除cry26Aa所在质粒的突变株BMB1151中表达,形成的晶体与芽胞也分别独立存在不能粘连,从而进一步推断幕虫亚种晶胞粘连现象与cry26Aa所在质粒有关。     

Molecular identification of Taenia spp. in wolves (Canis lupus), brown bears (Ursus arctos) and cervids from North Europe and Alaska

Taenia tapeworms of Finnish and Swedish wolves (Canis lupus) and Finnish brown bears (Ursus arctos), and muscle cysticerci of Svalbard reindeer (Rangifer tarandus platyrhynchus), Alaskan Grant's caribou (Rangifer tarandus granti) and Alaskan moose (Alces americanus) were identified on the basis of the nucleotide sequence of a 396 bp region of the mitochondrial cytochrome c oxidase subunit 1 gene. Two species were found from wolves: Taenia hydatigena and Taenia krabbei. The cysticerci of reindeer, caribou and one moose also represented T. krabbei. Most of the cysticercal specimens from Alaskan moose, however, belonged to an unknown T. krabbei-like species, which had been reported previously from Eurasian elks (Alces alces) from Finland. Strobilate stages from two bears belonged to this species as well. The present results suggest that this novel Taenia sp. has a Holarctic distribution and uses Alces spp. as intermediate and ursids as final hosts.     

Phylogenetic relationships of graminicolous downy mildews based on cox2 sequence data

Graminicolous downy mildews (GDM) are an understudied, yet economically important, group of plant pathogens, which are one of the major constraints to poaceous crops in the tropics and subtropics. Here we present a first molecular phylogeny based on cox2 sequences comprising all genera of the GDM currently accepted, with both lasting (Graminivora, Poakatesthia, and Viennotia) and evanescent (Peronosclerospora, Sclerophthora, and Sclerospora) sporangiophores. In addition, all other downy mildew genera currently accepted, as well as a representative sample of other oomycete taxa, have been included. It was shown that all genera of the GDM have had a long, independent evolutionary history, and that the delineation between Peronosclerospora and Sclerospora is correct. Sclerophthora was found to be a particularly divergent taxon nested within a paraphyletic Phytophthora, but without support. The results confirm that the placement of Peronosclerospora and Sclerospora in the Saprolegniomycetidae is incorrect. Sclerophthora is not closely related to Pachymetra of the family Verrucalvaceae, and also does not belong to the Saprolegniomycetidae, but shows close affinities to the Peronosporaceae. In addition, all GDM are interspersed throughout the Peronosporaceae s lat., suggesting that a separate family for the Sclerosporaceae might not be justified.