毛喉鞘蕊花的化学成分

从毛喉鞘蕊花分离得到两个新二萜醌 :鞘蕊酮S(1) ,鞘蕊酮T(2 )和六个已知成分 :α 雪松醇 ,愈创木酚甘油醚 ,芫花素 ,鞘蕊醇B ,β 谷甾醇 ,豆甾醇。依据光谱和化学方法 ,它们的化学结构已被鉴定     

黄鞘蕊花中两个新的醌式二萜

从黄鞘蕊花中得到２个新的醌式二萜（二聚体）,黄鞘蕊花乙素,黄鞘蕊花丙素和２个已知化合物蒲公英萜醇,齐墩果酸。     

我国毛喉鞘蕊花的发掘与研究进展

本文介绍了哮喘病新药毛喉鞘蕊花的发掘过程,栽培条件下的形态特征和化学成分与印度毛喉鞘蕊花的区别,结果显示,二者并非同种植物,特将云南种定名为小江鞘蕊花。     

鞘蕊花的组织培养

植物名称鞘蕊花(Coleus forskohlii)。材料类别:顶芽、侧芽。培养条件:取鞘蕊花幼嫩的顶芽和侧芽,经自来水冲洗后去叶,用0.1％的升汞灭菌5min,再用无菌水冲洗数次后切成0.5～1.0cm长的带芽茎段,接入诱导培养基上。基本培养基为MS并附加不同浓度的BA,NAA和GA_3(单位mg/L);培养     

毛萼鞘蕊花甲素的结构

从毛萼鞘蕊花(Coleus esquirolii(L(?)vl. )Dunn.)的茎、叶中分离鉴定了一个新的二萜化合物,毛萼鞘蕊花甲素(对映-17-乙酰氧基-贝壳杉烷-16β-醇),和三个已知化合物,对映-贝壳杉烷-16p,17-二醇,齐墩果酸和β-谷甾醇。     

黄鞘蕊花的化学成分

从黄鞘蕊花(Coleus xanthanthus C. Y. Wuet Y. C. Huang)中分得8种成分。其中1个是新的醌式二萜,命名为黄鞘蕊花甲素,其结构为17-methyl(15→16)abieo-16-methoxy-2α-acetoxy-coleon U.7个已知化合物。coleon U(2),sugiol(3),β—谷甾醇,胡萝卜甙,三十三烷醇,三十三烷和二十八烷酸。     

毛喉鞘蕊花的微量成分

从唇形科毛喉鞘蕊花全草的氯仿提取物分离到２个新的微量成分,鞘蕊花戊素和已素。基于光谱分析,鞘蕊花戊素和已 素的化学结构分别鉴定为１α,７β－二乙酰氧基－８,１３－环氧－６β羟基勒丹－１４烯－１１－酮（１）和７β－乙酰氧基－８,１３环氧－６β,α－二羟基勒丹－１４烯－１１－酮（２）。     

毛喉鞘蕊花愈伤组织的诱导及其活性成分的产生

本文简要报道了毛喉鞘蕊花(Coleus jorskohlii)无菌苗愈伤组织的诱导,初步培养及细胞悬浮培养,同时对培养细胞中的有效成分进行了初步分析。     

佛司可林类成分的光谱特征(一)

活性二萜佛司可林和异佛司可林已从毛喉鞘蕊花分离得到。本文详细描述了它们的光谱特征(包括一维和二维的核磁共振谱)。     

丫蕊花属植物花粉和种子微形态特征比较及其分类学意义

采用光学显微镜和扫描电镜,对4种丫蕊花属植物(丫蕊花、小果丫蕊花、云南丫蕊花和高山丫蕊花)的种子微形态特征及表面纹饰等进行观察,并对除云南丫蕊花外的3种丫蕊花属植物花粉形态、大小、表面纹饰等微形态特征进行观察和比较分析,并讨论了花粉及种子微形态特征的进化生物学意义,为丫蕊花属植物物种间的分类学鉴定提供理论依据。结果表明:(1)丫蕊花属植物花粉形态多为长球形,少数为超长球形,赤道面观为椭圆形,极面观为圆形,花粉大小为(22~36.5)μm×(11.5~24)μm,花粉外壁纹饰为刺状颗粒。(2)丫蕊花的花粉较大,极轴明显长于其余种,小果丫蕊花极轴长度最小。(3)花粉外壁刺状颗粒纹饰在4种丫蕊花之间存在差异性特征。(4)丫蕊花属植物种子细梭形,两端具纤维状长尾,种皮表面纹饰为复网纹纹饰。(5)云南丫蕊花的种子最大,种脊显著;小果丫蕊花种子最小。(6)组成种子表面网纹纹饰的网脊、网壁及网眼的表面特征等在各物种间存在一定差异。     

Phloem differentiation in Coleus pith explants

A simple tissue culture protocol was used to examine phloem differentiation. Sieve tube elements differentiated from Coleus blumei Benth. pith parenchyma when pith blocks were explanted on to an agar medium containing sucrose and indole acetic acid. Growth regulator concentration had surprisingly little influence on the production of phloem cells. Increasing the availability of sucrose, however, resulted in an enhancement of phloem differentiation. Evidently phloem and xylem differentiation began at the same time in this system, and the concentration of sucrose determined the relative production of phloem cells.     

High forskolin production in hairy roots of Coleus forskohlii

Hairy roots of Coleus forskohlii were induced by infection with the Agrobacterium rhizogenes MAFF 03-01724 strain. Growth and forskolin production of two hairy root clones cultured in various liquid media were examined. Hairy root clone B9 grew well in woody plant liquid medium and showed a high forskolin yield (ca. 1.3 mg/ 100 ml flask) after 5 weeks of culture. The time course of growth and forskolin production of the clone B9 cultured in woody plant liquid medium was also examined. Rapid growth started at week 2 and continued until week 5. The highest forskolin yield (ca. 1.6 mg/100 ml flask) was obtained at week 5. Productivity was much higher than that previously reported. Received: 19 June 1997 / Revision received: 6 October 1997 / Accepted: 18 October 1997     

毛喉鞘蕊花的化学成分研究

本文报导了,从产于滇东北的毛喉鞘蕊花(Coleus forskohlii Brig)的根茎中分离到四个二萜化合物,经光谱分析鉴定,其结构分别为1α,6β,7α-triacetyl-coleonol—B(1),I-acetyl—forskolin(2),coleonol-B(3),和deacetyl-forskolin(4)。化合物(1)和(2)是首次从该植物中分离到。     

毛喉鞘蕊花提取物对大鼠哮喘模型炎症介质的影响及机制研究

为探讨毛喉鞘蕊花提取物(Coleus Forskohlii extract,CFE)对哮喘的治疗作用及机制,采用卵清蛋白(ovalbumin,OVA)结合佐剂氢氧化铝建立大鼠哮喘模型,SPF级SD雄性大鼠60只,随机分为5组,即空白组、模型组、地塞米松组、CFE高剂量组(12.8g/kg)和CFE低剂量组(6.4g/kg),分别用生理盐水、地塞米松及CFE每天灌胃1次,连续14天,灌胃容积为1mL/100g。通过HE染色观察肺组织病理学形态学变化;ELISA法测定大鼠血清(serum)及肺泡灌洗液(BALF)中IFN-γ、IL-4和IL-17A含量变化;Western blot检测MAPK通路相关蛋白的表达变化。HE结果显示,与模型组相比,CFE高、低剂量组能显著减轻OVA刺激后大鼠肺组织病理性损伤,气道轮廓清晰,上皮细胞脱落明显减少;ELISA结果显示,与模型组相比,CFE高、低剂量组大鼠血清及BALF中IL-4、IL-17A的含量明显下降,IFN-γ的含量明显上升(P<0.05或P<0.01);WB结果显示,CFE高剂量组可显著降低大鼠肺组织p-ERK、p-JNK及p-p38蛋白表达(P<0.05或P<0.01)。综上,毛喉鞘蕊花提取物可有效缓解OVA诱导的大鼠哮喘气道炎症反应,其机制与调节MAPK信号传导通路有关。     

Effect of arbuscular mycorrhizal fungi and Pseudomonas fluorescens on root-rot and wilt,growth and yield of Coleus forskohlii

Root-rot and wilt caused by Fusarium chlamydosporum affects the cultivation of Coleus forskohlii, a medicinal plant grown for its roots, which contain a pharmaceutically important compound called forskolin. In this study, management of this disease under low and high inoculum levels was assessed with four arbuscular mycorrhizal (AM) fungi and a strain of Pseudomonas fluorescens. The AM fungus Glomus fasciculatum and P. fluorescens were the most effective treatments that reduced the severity of root-rot and wilt of C. forskohlii by 56–65% and 61–66%, respectively, under lower and higher levels of pathogen F. chlamydosporum. G. fasciculatum increased the dry shoot and root weight by 108–241% and 92–204%, respectively, while in plants treated with P. fluorescens, an increase of 97–223% and 97–172% in dry shoot and root weight, respectively, was observed. Although P. fluorescens was effective, it gave higher root yields only under lower inoculum level of the pathogen. G. fasciculatum performed equally well under both lower and higher inoculum levels. Increase in yields with both the biocontrol agents was accompanied by increase in P uptake (230–303%) and in K uptake (270–335%). The forskolin content of the roots was significantly increased (14–21%) by G. fasciculatum, P. fluorescens or G. mosseae under lower inoculum level of pathogen.