生物化学“2463”课堂教学设计与实践

生物化学是生命科学领域学科专业重要基础课之一,也是"教"与"学"的两难课程之一。基于国内外教学理论和农学专业课程多年的实践教学经验,构建了生物化学课程的"2463"教学模式:在师生双主体的前提下,在教学内容、教学方法、教学手段和教学评价四个层面开展了六个目标方向的实践探索,即教学内容的前沿化、生活化和人文化,教学方式的多元化,教学手段的信息化和教学评价的过程化,而且每个目标方向至少进行三个路径的实践探索。该教学模式经过五轮的教学实践,取得了良好的教学效果。     

生物化学教学方法的几点体会

生物化学是生命科学的领头学科,有大量的知识点需要记忆,也是生命科学领域一门难教难学的课程之一。在教学中采取联想记忆、总结记忆、问题式等多种教学方法,增强学生学习兴趣和学习主动性,是提高教学效果的有效途径。     

食品科学与工程专业生物化学教学体会——从应用型人才培养角度出发

生物化学是生命科学、食品科学、医学等多个领域相关专业的基础课。笔者从食品科学与工程专业应用型人才培养的角度出发,对人才培养目标、教学内容、教学方法与手段和教师的"爱与激情"等几个方面进行了探讨,以期更好地指导、促进生物化学的教学工作。     

生物化学教学与课程建设的体会

生物化学是生命科学学科中一门重要基础课程,对该专业本科生夯实基础、学好专业、未来发展起着关键作用.基于长期教学及教学改革的实践,对生物化学课程从教学内容、教学形式、教学方法和手段的完善与更新进行了探索,并提出了一些具体建议,旨在为该课程教学质量的提高提供参考.     

《生物分离技术》课程教革的体会

生物分离技术是在生物学、细胞学、微生物学、生物化学、化学、化学工程、物理化学、机械和电子等学科的有关技术的基础上发展起来的 ,是生物工程专业的必修课程 ,因此该课程的教学直接影响了学生的专业素质 ,作者多年从事该课程的教学与科研 ,深刻体会到激发学生的学习兴趣 ,丰富教学内容 ,改革教学方法 ,改进教学手段 ,强调学生能力的培养 ,加强理论与实践相结合 ,是教好这门课程的关键 ,下面从几个方面总结十几年的教革体会。1　培养和激发学生的学习兴趣常言道兴趣是最好的教师。培养学生对课程的学习兴趣 ,是学生能否学好这门课程的关…     

研究生高级生物化学课程教学改革的实践与思考

高级生物化学是食品科学与工程等相关专业研究生教学过程中的一门重要的专业基础课。针对目前研究生高级生物化学课程教学中存在的问题,本文在课程教学知识体系、教学方法与手段、考核评价方式等方面进行了教学改革的实践探索,以期为研究生高级生物化学教学实践和改革提供借鉴。     

中医院校《生物化学》教学方法改革与实践的体会

由于学科特点、学生生源的化学基础及中医药大学对西医课程的教学定位等因素的影响,生物化学课程的教与学一直面临挑战。为切实提高生物化学的有效教学,我们在生物化学教学方法上进行了一些探索、改革与实践,收到了良好的效果,与大家一起交流和分享。     

基于CiteSpace生物化学实验教学动态与热点可视化分析

生物化学是生物学和医学学科非常重要的基础课程,是进入21世纪以来发展最为迅速和最具活力的学科之一.生物化学理论教学极具抽象性,所以其实验教学是理解相关理论与掌握实际技能的重要环节.在生物化学实验教学过程中,及时掌握新的教学理念,新的教学方法,新的教学热点,紧跟教学发展趋势一直是教师们关注的重点问题.本文以中国学术期刊网...     

《汉英生物化学词汇》评介

生物化学是生物科学的一门重要基础学科。生物化学重点研究生物大分子(核酸、蛋白质、酶等)的组成、结构、功能、代谢、调控及能量转换等。随着科学的迅速发展,生物化学与生物科学的其他分支学科发生了广泛的互相渗透与交叉。特别是生物化学技术逐渐成为一项通用手段,被生物科学各分支学科广泛采用。生物化学知识对于从事生物科学研究、教学及与生物     

《生物化学》教学方法发展概况

《生物化学》是一门十分重要却难以掌握的基础课程。在教学实践中,高校教师一直在探索并应用各种教学方法来提高教学质量。传统的教学方法有讲授法、多媒体结合板书法、直观式教学法、案例式教学法等。近年来被不断探索和尝试的新兴教学方法和教学形式有PBL教学法、微课、翻转课堂、Sandwich教学法、Seminar式教学法等。本文将对以上教学方法及其在《生物化学》课程中的应用加以综述。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.