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利用发光二极管(LED)作为光源,以冷百荧光灯光作为对照,研究不同光质[红光637 nm、绿光529 nm、蓝光453 nm、白光(400700) nm]对念珠藻葛仙米生长和生理生化特性的影响。结果表明:在培养前期,红光促进藻蓝蛋白合成,而藻红蛋白合成受抑制;蓝光和绿光则促进藻蓝蛋白合成。在培养后期,红光处理有利于叶绿素a和类胡萝卜素积累,其含量分别达到干重的1.33%和0.24%;绿光、白光和冷白荧光培养物的相应色素的含量均约占1.0%和0.16%;蓝光培养物的相应色素含量分别仅为0.45%和0.11%。红光培养物的氨基酸含量达干重的23.1%,是对照的1.58倍。除蓝光外其他光质对还原糖的含量影响无显著差异。在培养过程中LED白光和冷白荧光培养物的平均相对生长速率分别约为其他色光培养物的1.3和1.5倍。 相似文献
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光质对湛江等鞭金藻生长和脂肪酸组成的影响 总被引:1,自引:0,他引:1
在气升式光生物反应器中研究不同光质光影响湛江等鞭金藻的生长。结果表明,藻细胞密度的大小顺序为:红光〉白光+红光、白光〉白光+蓝光+红光〉白光+蓝光〉蓝光。蓝光下多不饱和脂肪酸百分含量最高,占总脂肪酸的50.01%。白光下总单不饱和脂肪酸和总饱和脂肪酸含量最高,占总脂肪酸的24.19%和27.46%。多不饱和脂肪酸中C18:4。.3含量最高,占总脂肪酸的20.3%-23.3%,最高值出现在蓝光下;其次为C22:6n-3(DHA),占总脂肪酸的10.2%-12.3%,在蓝光和白光+蓝光中较高;而C18:2n-6和C18:3n-3均以红光下的为最高,分别达3.11%和8.04%。 相似文献
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[背景]环境因子和营养因子对微藻的生长和生化组成都有显著的影响,其中光强和氮浓度是最重要的两个条件。[目的]研究不同光强和初始氮浓度对丝状绿藻-双星藻(Zygnema sp.)生长及生化组成的影响。[方法]采用改良的BBM培养基,设置了两组光强[100μmol/(m2·s)和300μmol/(m2·s)]和6种初始氮浓度(3、6、9、12、15和18 mmol/L)在柱状光生物反应器中对双星藻进行培养。[结果]在高光强条件下[300μmol/(m2·s)],12 mmol/L初始氮浓度最有利于双星藻生物质的积累,其最高生物量可以达到6.60 g/L,而初始低氮浓度(3 mmol/L)则促进了油脂和脂肪酸的积累,油脂最高含量占干重的32.13%,且脂肪酸组成主要包括棕榈酸(C16:0)、油酸(C18:1)、亚油酸(C18:2)和亚麻酸(C18:3),其中油酸含量最高达到总脂肪酸含量的55.01%;在低光强条件下[100μmol/(m 相似文献
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本文讨论了将光质应用于一品红(Euphorbia pulcherrima)大规模细胞繁殖及形态发生调控的可行性,并测定了一些形态及生化指标作为这种调控的参数。 以一品红试管苗的叶片作为外植体,置于MS培养基中并分别培养于白(W)、红(R)、黄(Y)、蓝(B)、绿(G)色光及黑暗(D)下,一个月后进行测定。蓝光和黄光明显促进出苗。而愈伤组织形成的情况则正相反,红光利于愈伤组织的形成,黄光效果最差。过氧化物酶、过氧化氢酶活力及蛋白质、RNA、碳水化合物的含量与愈伤组织形成有较为一致的关系,但与分化不直接相关。 实验中还测定了叶绿素a、叶绿素b及类胡萝卜素的含量。白光与蓝光均能促进叶绿素及类胡萝卜素的合成,但蓝光中chl b/chl a的比率较高。此外,本文尚涉及暗处理中的晶质化现象(vitrification)及其成因。 相似文献
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光质对油菜幼苗生长及抗氧化酶活性的影响 总被引:1,自引:0,他引:1
用40 μE·m-2·s-1的白光和红光对油菜幼苗照光处理9 d,结果表明,红光促进油菜幼苗的生长,使其抗坏血酸过氧化物酶(APX)活性明显升高,过氧化物酶(POD)活性下降,但对过氧化氢酶(CAT)活性无明显影响。 相似文献
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利用发光二极管(LED)作为光源,以冷百荧光灯光作为对照,研究不同光质[红光637 nm、绿光529 nm、蓝光453 nm、白光(400—700)nm]对念珠藻葛仙米生长和生理生化特性的影响。结果表明:在培养前期,红光促进藻蓝蛋白合成,而藻红蛋白合成受抑制;蓝光和绿光则促进藻蓝蛋白合成。在培养后期,红光处理有利于叶绿素a和类胡萝卜素积累,其含量分别达到干重的1.33%和0.24%;绿光、白光和冷白荧光培养物的相应色素的含量均约占1.0%和0.16%;蓝光培养物的相应色素含量分别仅为0.45%和0.11%。红光培养物的氨基酸含量达干重的23.1%,是对照的1.58倍。除蓝光外其他光质对还原糖的含量影响无显著差异。在培养过程中LED白光和冷白荧光培养物的平均相对生长速率分别约为其他色光培养物的1.3和1.5倍。 相似文献
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光质对水稻幼苗生长及生理特性的影响 总被引:7,自引:0,他引:7
以荧光灯为对照,采用发光二极管(light emitting diode,LED)精确调制不同光谱能量分布,研究了光质对‘武运粳7号’和‘抗优63’两种水稻幼苗生长及生理特性的影响.结果表明:光质对两个品种水稻幼苗生长有显著影响且存在差异.蓝光显著抑制幼苗株高,提高‘武运粳7号’叶片的可溶性蛋白含量及两个品种水稻五叶期幼苗的壮苗指数;红光显著提高三叶期幼苗的茎基直径、壮苗指数以及五叶期叶片的可溶性糖和淀粉含量;红蓝组合光显著提高三叶期幼苗的根数、茎基直径、壮苗指数、根系活力和可溶性糖含量,以及五叶期幼苗的鲜、干质量、壮苗指数、叶片可溶性糖和蔗糖含量;黄光可在幼苗生长初期明显增加株高,提高叶片色素含量.总体上,红蓝组合光有利于培育水稻壮苗. 相似文献
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E. Aidar S.M.F. Gianesella-Galvo T.C.S. Sigaud C.S. Asano T.H. Liang K.R.V. Rezende M.K. Oishi F.J. Aranha G.M. Milani M.A.L. Sandes 《Journal of experimental marine biology and ecology》1994,180(2):175-187
The effects of light quality on growth, biochemical composition and photosynthetic production in Cyclotella caspia Grunow and Tetraselmis gracilis (Kylin) Butcher were evaluated under controlled laboratory conditions. Cyclotella caspia had the highest values for maximum growth rate in blue-green light, whereas T. gracilis grew faster in red light. The highest cellular contents of chlorophylls [a, (c1 + c2)] and carotenoids of C. caspia were found respectively in red and blue-green light, while protein content did not change in response to spectral quality. Tetraselmis gracilis cells were more stimulated to synthesize pigments and protein when incubated in white light. For both species, pigment ratios showed intermediate values in white regime. The maximum values for photosynthetic rates were obtained in blue-green and red regimes in C. caspia and in red light in T. gracilis. The chromatic adaptive mechanisms shown for both species are compared and discussed in light of recent works presented for different phytoplankters, with emphasis on ecophysiological responses obtained in distinct spectral regimes. 相似文献
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Gassan Hodaifa Ma Eugenia Martínez Sebastián Sánchez 《Engineering in Life Science》2010,10(3):257-264
Scenedesmus obliquus can help to reduce the environmental impact of industrial olive mill wastewater from olive oil extraction in the three‐phase system. This work examines the effect of temperature changes (288–308 K) on algal growth, culture medium, and biochemical composition of S. obliquus. The maximum specific growth rate of 0.024 h?1 occurred at an optimal temperature of 302.7 K. The apparent activation energies of cell growth and cell death were determined as 61.8 and 142.8 kJ/mol, respectively. At the end of culture the percentages of pigments, proteins, and carbohydrates were greater at the two ends of the temperature range studied, as it was also observed for the maximum elimination of biochemical oxygen demand (BOD5). The mono‐ and polyunsaturated fatty acid content of the biomass was greater at the lowest temperature used (288 K). 相似文献
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Summary The existence of two photoreceptors regulating chloroplast orientation was found in the centric diatomPleurosira laevis. Chloroplasts migrate through the transvacuolar cytoplasmic strands according to the light conditions. Weak white light of less than 46 mol/m2 · s (10 W/m2) induces chloroplast movement to the cortical cytoplasm, which is located next to the plasma membrane (dispersion), while intense white light of more than 92 mol/m2 · s (20 W/m2) induces chloroplast movement towards the nucleus, which is situated in the center of the cell (assemblage). Chloroplast dispersion was maintained as long as the cells were irradiated with weak white light. Conversely, chloroplast assemblage under intense white light was transient and the chloroplasts were released from assemblage after 15 min. Action spectra determined with the Okazaki Large Spectrograph revealed that the weak white light receptor and the intense white light receptor are characterized by 540 nm and 450 nm optima, respectively. 相似文献
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Growth and pigment composition in the red alga <Emphasis Type="Italic">Halymenia floresii</Emphasis> cultured under different light qualities 总被引:1,自引:0,他引:1
José Luis Godínez-Ortega Pauli Snoeijs Daniel Robledo Yolanda Freile-Pelegrín Marianne Pedersén 《Journal of applied phycology》2008,20(3):253-260
Halymenia floresii is an edible species consumed in some Asian markets. In the Yucatan peninsula coast of Mexico, H. floresii dominates rocky substrata between 3 and 40 m where it grows up to 50 cm high. After analyzing the seasonal pattern of pigment
content on H. floresii, we evaluate if and how the spectral composition of light affects growth and pigment dynamics under laboratory cultivation.
Unialgal cultures were exposed to white, blue, red and green light in a 3-week experiment. Green light resulted in the highest
algal growth rates. Synthesis of chlorophyll a, α-carotene and lutein, but not of β-carotene, was induced by white or green light. Phycocyanin synthesis was stimulated by blue light and phycoerythrin synthesis
by blue or red light. Light quality treatments may be used to manipulate pigment composition in Halymenia floresii cultures. 相似文献
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不同氮素水平对产油尖状栅藻生长及光合生理的影响 总被引:1,自引:0,他引:1
在300μmol photons·m-2·s-1光照下,以不同初始氮素营养条件的改良BG-11培养基为基础培养基,在Ø6 cm的玻璃柱状光生物反应器通气(加富1.5% CO2)培养绿藻尖状栅藻(Scenedesmus acuminatus),分析探讨藻细胞的生长及光合生理与氮素营养的关系。结果表明,不同氮素实验组藻细胞的最大生物量有差异,氮浓度为6mmol/L实验组的生物量最高,为5.19g/L。与初始氮浓度18 mmol/L相比,较低的氮浓度9 mmol/L、和 6 mmol/L 在培养前期对尖状栅藻的生长具有明显的促进作用。藻细胞叶绿素a、b及总类胡萝卜素含量与培养液的氮素营养水平呈正相关。低氮条件有利于总脂积累,总脂含量和单位体积总脂产率显著高于全氮组(P<0.05),3.6 mmol/L实验组的总脂含量最高,为干重的54%,比全氮组高17%(P<0.05)。培养后期随着藻细胞总脂的积累,总碳水化合物和总蛋白含量明显下降(P<0.05)。PSⅡ最大光能转化效率(Fv/Fm)、实际光能转化效率(Yield)以及相对电子传递效率(ETR)均随氮素限制而显著下降(P<0.05),光合速率在不同生长阶段呈先上升后下降的趋势,呼吸速率在培养周期内缓慢上升,说明藻的生长、油脂的积累与细胞光合生理状况以及氮素营养水平直接相关。 相似文献
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Lizelotte Leeflang 《Plant Ecology》1999,141(1-2):59-65
An experiment was set up to test whether stoloniferous plants are able to avoid neighbours by means of asymmetric growth in response to low red:far-red (R:FR) ratios in light that is reflected by neighbouring plants. Neighbours were simulated by green mirrors that were placed on the north-side of the plants while spectrally neutral mirrors served as a control. Six stoloniferous species (Trifolium repens L., Hydrocotyle vulgaris L., Lysimachia nummularia L., Glechoma hederacea L., Lysimachia nemorum L. and Lamium galeobdolon L.) were included in the experiment. Measurements were made on branching frequency, timing of branching, stolon growth rate, internode length, growth direction and biomass distribution. Although differences between treatments were found in some of these parameters, they were too small to lead to the avoidance of the simulated neighbours. Only L. galeobdolon produced lower biomass in response to the green-mirror treatment compared to the control one. 相似文献
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In order to understand the molecular mechanism of growth regulation in phytoplankton and to develop novel growth-status indicators, a subtraction cDNA library was constructed by using the mRNA extracted from Skeletonema costatum in the rapid-growth stage (RG stage), and three RG-stage-related cDNA fragments, RG#14, RG#25, and RG#42, were obtained. According to the results of sequence analysis, RG#42 belonged to the MCM2-7 protein family, and the other two fragments, RG#14 and RG#25, were novel molecules. Under continuous illumination, these RG-stage-related mRNA expression levels increased from 100- (RG#14 and RG#42) to 1,000-fold (RG#25) with increasing growth rate. Furthermore, under a diel rhythm of light (light-dark = 12:12 h), the daily mean mRNA abundances of RG#14 and RG#25 in the exponential phase also differed from those in the late-stationary phase. However, such differences between these growth phases were not observed in the mRNA levels of RG#42 and PCNA. This study not only provided a new way to investigate the regulatory mechanisms of cell growth but also offered a possibility of employing these gene fragments as indicators to monitor the growth status of phytoplankton in the marine environment. 相似文献