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1.
麦角固醇产生菌Torullopsis famata优化发酵条件的研究   总被引:11,自引:0,他引:11  
从12株产麦角固醇的酶母菌株中,筛选出一株麦角固醇产量较高的酵母菌(无名球拟酵母Torullopsis famata),并对其产麦角固醇的条件进行了研究,结果表明:麦角固醇发酵的最佳条件为以蔗糖及复合氨源(黄豆粉+NaNO3)为C、N源,温度28℃,pH5.5,装量250ml三角瓶装20ml,时间96h。麦角固醇的含量可达细胞干重的1.80%,平均产量可达74mg/100ml。  相似文献   

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目的:通过发酵条件优化,提高海洋Cellulophagasp.QY201的l-卡拉胶酶产量。方法:采用正交试验分别对发酵条件、培养基配方进行优化。结果:该菌株最适培养基配方为(w/v):3%NaCl、0.5%MgSO4·7H2O、0.02%CaCl2、0.01%KCl、0.002%FeSO4、0.25%CaSein、0.15%Na2HPO4、0.2%NaNO3、0.25%L-卡拉胶。最佳培养条件为:培养基体积为70ml/250ml三角瓶,接种量为1%,25℃,90r/min培养36h。优化后酶活最高可达2.64U/ml,较优化前提高了22倍。结论:QY201最佳发酵条件的建立,为ι-卡拉胶酶的大规模生产创造了条件。  相似文献   

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对1株产抑菌物质的海洋真菌Caldariomyces fumago S-5的培养条件的优化进行研究,探讨该菌株的发酵产抑菌物质的性能。通过抗菌谱实验、单因素及正交实验设计研究了该菌株所产抗菌物质的押菌特征和最适发酵条件。结果表明,S-5菌株发酵液对几种G^+和G^-致病细菌具有快速的生长抑制作用,而对真菌没有明显抗菌性。发酵条件优化结果表明:菌株在葡萄糖4%、(NH4)2SO4 0.2%、KCl 0.2%、K2HPO40.2%、Fe2SO4·7H2O 0.002%、MgSO4-2H2O 0.1%、20%马铃薯浸出液的培养基中,控制发酵温度25℃,pH值5.0,接种量1-2cm。菌苔/100mL培养液,摇瓶转速240r/min条件下培养120h,发酵液中抑菌效价最高。实验结果可为该海洋微生物资源的开发利用提供依据。  相似文献   

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目的对产类细菌素嗜酸乳杆菌P302的发酵条件进行研究。方法采用琼脂扩散法测定发酵上清液对大肠埃希菌O139的抑菌活性。结果通过正交试验确定P302产类细菌素的最佳培养基组分为牛肉膏4%、胰蛋白胨0.2%、酵母粉0.8%、碳酸钙0.4%和蔗糖1%,0.4%复合维生素,0.3%Tween-80有利于类细菌素的产生。发酵工艺条件为最适初始pH7.0,最适温度37℃,最佳接种量0.3%,种龄14h,厌氧培养22h。结论通过优化发酵条件和发酵工艺,类细菌素的产量提高了35%。  相似文献   

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对产竹红菌素的菌株SUPER-H168的ITS-5.8S rDNA进行了序列测定和进化树聚类分析,表明其属于竹黄属;对该菌株固态发酵产竹红菌素的条件进行了初步优化,得到的初步优化条件为玉米渣25g,麸皮5g,葡萄糖1.5g,NaNO3 0.15g,ZnSO4·7H2O 0.03g,起始含水量为50%,起始pH为7.0,培养温度为30℃。在该条件下发酵18d,竹红菌素的产量为9.37mg/g干基。  相似文献   

6.
固态发酵生产腺苷酸脱氨酶   总被引:1,自引:0,他引:1  
对多株曲霉产腺苷酸脱氨酶的性能进行了比较,发现米曲霉3.800(Aspergillus oryzae)产酶水平较高。该菌株固态发酵产酶的适宜培养基为:以麸皮为主原料,蔗糖2%,鱼粉2%,(NH4)2SO4 0.1%,柠檬酸钠0.2%,MgSO4 0.05%,吐温-80 0.1%,含水量50%。最佳的培养条件为:250mL三角瓶装20g培养基,在28-30℃培养60h。在优化条件下,培养物酶活可达到1543.48u/g鲜曲。  相似文献   

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发酵戊糖产酒精酵母菌株的选育   总被引:9,自引:0,他引:9  
介绍了一种新的发酵戊糖产酒酵母菌种筛选方法并利用该方法筛选出一株性能优良的发酵戊糖酵母蓖株Z8,该菌株性能测试结果为:产酒精能力相当于发酵戊糖理论产量的60.0%,耐酒精能力14%(V/V),在温度高达42℃的条件下仍能正常发酵,初步鉴定该菌株为管囊酵母(Pachysolen tannophilus)。  相似文献   

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以细脚拟青霉RCEF0969菌株的抗菌活性为指标对该菌株的液体发酵条件进行优化得到最佳培养基为:4%白砂糖、0.5%黄豆粉、0.5%蛋白胨、0,006%MnSO4·H2O、0.05%MgSO4·7H2O、2%麦芽汁。并且得到最佳发酵工艺条件为:斜面培养4d的种子作为摇瓶种子,以10%的接种量接种于装液量为2/5的三角瓶中,培养温度为28℃,摇瓶转速为160r/min,培养时间为6d。  相似文献   

9.
蜡状芽孢杆菌S1发酵条件的研究   总被引:4,自引:0,他引:4  
对一种新近分离的蜡状芽孢杆菌(Bacillus Cereus)菌株S1发酵产新型抗真菌多肽APS的发酵培养基组成(碳,氮源)和工艺条件(发酵温度,初始,PH,通气方式和通气量)进行了摸索,通过单因互实验和正交优化实验,确定了S1发酵培养基的组成。麸皮5%,玉米粉5%,尿素0.2%,或NH4NO31.5%,酵母浸亮1.5%,葡萄糖6%,NaCl0.1%;最适发酵培养温度为28度;最适发酵培养初始Ph为7.4或6.8,。在优化条件下,效价最高为8-10mg/ml,S1生长的最适温度和初始PH为30度和7.0,通气对S1发酵具有显著的影响。  相似文献   

10.
产氨短杆菌(Brevibacterium ammoniagenes)B1-15-15,为野生型,需生物素,对Mn++不敏感菌株,它具有“分段合成”核苷酸的能力,将外源核酸碱基转变为相应的核苷酸类物质。在30℃摇床培养3天后,加入0.3—0.5%的次黄嘌呤继续培养2天,可产生5'-IMP12一16克/升。发酵的适宜条件为:培养基分别灭菌前的pH,糖镁液为5.7(自然pH),磷氮液为7.0;发酵过程中补加尿素控制pH6.7—7.0,尿素总量0.7%左右;玉米浆用量3—4%;Mn++ 50一100微克/升。在30℃培养2天,补加0.4%尿素和0.4%次黄嘌呤,提高温度至37℃培养,可缩短发酵周期至2.5天,5'-IMP产量为9.54克/升。  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

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正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

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