大乳头水螅在中国的首次发现

淡水水螅有效物种已记述36种。史新柏等(1987)对哈尔滨的强壮水螅Hydra robustaIto,1947作过报道;范学铭报道了中国水螅4个新种,即哈尔滨水螅Hydra harbinensisFan、漠河水螅Hydra mohensisFan,1999、大庆水螅Hydra daqin-gensisFan,2000和北京水螅Hydra beijingensisFan,2003,     

水螅

水螅是淡水中习見的一类腔腸动物,属于水螅綱(Hydrozoa),螅形目(Hydroida),裸芽亚目(Gymnoblasta),水螅科(Hydroidae)。本科包括:水螅属(Hydra)、柄水螅属(Pelmatohydra)、绿水螅属(Chlorohydra)和原水螅属(Protohydra)等。前二属分布較为普遍。     

五种水螅基因组DNA的RAPD多态性研究

利用RAPD技术对水螅科2属5种水螅进行了基因组DNA多态性研究.80个随机引物筛选出18个引物,共扩增出303个清晰稳定的DNA片段,分子量在0.3～3.0kb之间.以UPGMA聚类法构建树状图,阐明水螅属种的分类关系:大庆水螅Hydra daqingensis、北京水螅H.beijingensis和强壮水螅H.robusta 3种分成两组并聚在一起;不同属的漠河水螅H.mohensis和绿水螅Chlorohydra sp.聚为一支;由此2属5种水螅分为两个支系.     

异种嫁接的水螅畸形体初步观察

摘要：把强壮水螅（Hydra robusta）和普通水螅（H.vulgaris）除头后进行异种切口嫁接实验,共获得3例畸形体,对其进行280 d的培养,获得78只畸形子代。追踪观察发现：①畸形体及其无性生殖子代均具有2种钩刺丝囊（强壮水媳钩刺丝囊和普通水螅钩刺丝囊）;②其身体伸展时最长达52 mm;③无性出芽生殖的平均...     

雌雄同体的水螅其精子为什么不能和自身的卵细胞结合,而要与其他水螅的卵细胞结合呢?

答:淡水水螅在世界上已发现4属,即原水螅属、水螅属,柄水螅属和绿水螅属。水螅属46种,柄水螅属4种,绿水螅属2种,原水螅属1种。原水螅属的水螅不具触手,且为雌雄异体,其有性生殖方式为异体受精,这属在我国尚未发现。其余3个属的水螅均为雌雄同体,也就是说同一个水螅体上既有精巢又有卵巢。但由于同一水螅体上的精巢中所产生的精子和卵巢中所产生和卵细胞并不同时成熟,所以精子无法与本身的卵细胞结合成受精卵。然而对同一水域环境中的不同个体来说,情况就不同了,在某一些     

水螅和淡水水母

水螅水螅是生活在淡水里的腔肠动物,在我国各地都可以采到。在教学上是很重要的,是很好的实验材料。在我国最常见的是雌雄异体的柄水螅,即Pelmatohydra oligactus Schutze。雌雄同体的水螅Hydra vulgaris Pallas在华东各地可以采到。另一种与绿藻共生的绿水螅Chlorohydra viridissima Schutze,在昆明曾发现过。除了以上三种,国内可能还有其他的种,目前还没有人做过系统的调查。     

中国水螅属一新种(水螅纲,水螅科)

报道采自广东省肇庆市的淡水水螅1新种,多形水螅Hydra polymorphus sp.nov.,研究标本均来自1只水螅的单系繁殖群体,测量数据经生物统计学处理。新种的钩刺丝囊外形及内部刺丝盘旋均有多种形态,故新种名以此命名。所有研究标本保存于深圳大学生命科学学院。     

中国水螅属一新种(螅形目,水螅科)

记述水螅科水螅属1新种：哈尔滨水螅Hydra harbinensis sp.nov.。本种与其它已报告的雌雄同体水螅有明显区别,只与美洲的海曼水螅较为相似,但也有不同,其与海曼水螅的区别为：生活于静水,能直立并可横向附着生活,实验室培养用储放的自来水可正常生活及生殖。粘刺丝囊小型。胚鞘的鞘膜表面有网格,每根圆锥形的棘刺发自每一网格中央,棘刺无结节,不分叉。精巢数目为2～6个,卵为4～6个。模式标本保存于哈尔滨师范大学生命与环境科学学院生物学系无脊椎动物研究室。     

淡水水螅对环境因子的要求与适应

水螅是淡水生活的低等动物 ,是动物学教学和科研不可缺少的实验材料。作者通过对实验室内常年培养的4种水螅 (漠河水螅、太阳水螅、大庆水螅、哈尔滨强壮水螅 )的观察 ,浅淡水螅对生活环境的要求与适应特点。1　水质　水螅对其生活环境的水质要求比较严格 ,培养用水的清洁度对水螅的生存有直接的影响。用储放 1周以上的自来水或用充氧泵充氧 8h以上的自来水为培养用水 ( p H在 7.4～ 8.0之间 ) ,4种水螅都能正常生活。投喂食物后 ,需及时清除食物残体及排遗物 (不超过2 4 h为宜 )。清除如不及时 ,霉菌污染水质 ,造成水中乏氧 ,水螅的生存则…     

中国水螅属一新种(螅形目,水螅科)

记述采自北京地区水螅属1新种：北京水螅Hydra beijingensis sp．nov．。新种的主要鉴别特征是出现体部与柄的分化;钩刺丝囊内刺丝缠绕具有2种形态：大部分钩刺丝囊(占2／3左右)内顶端基部刺丝呈4～5圈横向弹簧状缠绕,而占所观察刺丝囊总数1／3左右的囊内刺丝为不规则缠绕和纵向缠绕。标本保存在哈尔滨师范大学生命与环境科学学院生物学系无脊椎动物研究室。     

中国绿水螅分子系统发生地位的初步探讨

目的:初步探讨中国绿水螅(Hydra sinensis)分子系统发生地位以及水螅属内部各类群系统发生关系。方法:采用酚-氯仿法提取中国绿水螅总DNA,扩增线粒体COI和16S r RNA基因片段并进行DNA序列测定,再利用Clustal及MEGA等生物信息学分析软件进行系统发生分析。结果:在本研究重建的所有系统发生树中,中国绿水螅始终与绿水螅Hydra viridissima的不同种群一起构成绿水螅单系群。同时,棕色水螅群的单系性被基于COI基因的NJ树以及基于16S r RNA基因的NJ树和ML树支持,唯独基于COI基因的ML树不支持棕色水螅群的单系发生。在基于COI基因的ML树中纤弱水螅族在系统树的基部独立为一支系,而绿水螅群和其他棕色水螅群水螅一起组成另一支系,提示纤弱水螅族水螅的系统发生地位值得进一步探讨。值得注意的是,根据本文的结果,棕色水螅群内3族的划分仍然有一定疑问。基于COI基因的NJ树和ML树支持普通水螅族、寡水螅族和纤弱水螅族各自族内的单系发生,但16S r RNA基因的NJ树和ML树中仅普通水螅族水螅聚为单系群,而寡水螅族和纤弱水螅族水螅各自并非单系发生。结论:把水螅属划分为绿水螅群及棕色水螅群有一定的合理性,但棕色水螅群内寡水螅族、普通水螅族和纤弱水螅族3族的划分还有待商榷。     

光周期对中国水螅种群增长、无性出芽生殖及抗氧化酶活力的影响

单细胞真核绿藻在中国水螅(Hydra sinensis)内胚层皮肌细胞中共生是有较高科研价值的特殊生物学现象。水螅宿主细胞为共生藻提供CO2、氮源及矿物质,而共生藻通过光合作用可能为宿主提供碳水化合物等有机物营养,因此水螅与共生藻间代谢流是以共生藻光合作用为中心,但基于代谢流二者间的互作机制目前尚未阐明。水螅通过营养积累进行出芽生殖,从母体脱落的芽体数量间接反映水螅营养积累的相对量。而光暴露时长能影响共生绿藻光合作用,如果共生藻的确能向水螅细胞转移光合作用产物,那光暴露时长应该能间接影响水螅的营养积累、从而进一步影响水螅无性出芽生殖。为证实该假说,本研究应用种群累积培养法,观察了光周期对中国水螅种群增长、无性出芽生殖及抗氧化酶(SOD和CAT)活力的影响。结果显示,光周期对中国水螅种群增长具有明显的影响。培养15 d后,所有实验组水螅的种群密度均为正增长,其中8L∶16D(在一个24h周期内光暴露8 h、黑暗16 h)实验组种群密度最大、而0L∶24D(持续黑暗)实验组种群密度最小。另外,随着光暴露时长的增加,中国水螅SOD及CAT活力整体均呈下降趋势。结果表明,从光周期对中国水螅无性出芽生殖及两种抗氧化酶活力的影响来看,中国水螅对光周期的生理学响应较为敏感,这个现象可能源于共生绿藻能通过向宿主细胞转移光合作用产物的方式为水螅提供营养补充。     

普通水螅(无鞘螅目,水螅科)中国一新纪录种

对采自中国广东省珠江流域(114°E,23°N)的一种淡水水螅进行连续两年的培养与观察,并扩增了其线粒体细胞色素氧化酶亚基Ⅰ(COⅠ)的基因序列.通过形态学分析及CO Ⅰ基因序列的鉴定确定其为普通水螅Hydra vulgaris Pallas,1766,为中国一新纪录种.     

Taxonomy of the European Hydra (Gnidaria: Hydrozoa): a re-examination of its history with emphasis on the species H. vulgaris Pallas, H. attenuata Pallas and H. circumcincta Schulze

The name Hydra attenuata Pallas is currently applied to the wrong animal. The common brown polyp, which is widely called H. attenuata, was described by Pallas (1766) as Hydra vulgaris. The name H. attenuata Pallas originally referred to an uncommon pale polyp, currently known as H. circumcincta Schulze. The history of this confusion is analysed here. The taxonomy of hydra was in disarray during the 18th and 19th centuries, and was clarified in 1917 with the monograph of Schulze. But Schulze misapplied the name for the common hydra, H. vulgaris, to an unusual form and thus was led to assign the name of a rare hydra, H. attenuata, to the common type. Schulze redescribed the rare, pale hydra that Pallas had named H. attenuata as H. circumcincta. The correct name of the common European brown, stalkless hydra is thus H. vulgaris Pallas, 1766. The name H. attenuata has priority for the uncommon pale hydra, but because of disuse of this application of the name, the pale hydra should be recognized by the current, generally accepted binominal H. circumcincta Schulze, 1914.     

Genome sizes and chromosomes in the basal metazoan Hydra

Hydras belong to one of the earliest eumetazoan animal groups, but to date very little is known about their genome sizes, gene numbers, and chromosomes. Here we provide genome size estimates and corresponding karyotypes for five Hydra species. Nuclear DNA contents were assessed by slide-based Feulgen microphotometry. Hydra oligactis possesses the largest genome of 1450 Mbp, followed by similar 1 C capacities in H. carnea (1350 Mbp), H. vulgaris (1250 Mpb) and H. circumcincta (1150 Mbp). The smallest genome of 380 Mbp was determined in H. viridissima. While the number of chromosomes is identical in all five Hydra species (2n = 30), the size of the chromosomes is strictly correlated to the size of the genome, with H. viridissima having conspicuously small chromosomes. The taxonomic and evolutionary significance of the C-value and chromosomal size variation in this ancient group of metazoans as well as its impact on genomic organization and forthcoming genome projects are discussed.     

Germline stem cells and sex determination in Hydra

The sex of germline stem cells (GSCs) in Hydra is determined in a cell-autonomous manner. In gonochoristic species like Hydra magnipapillata or H. oligactis, where the sexes are separate, male polyps have sperm-restricted stem cells (SpSCs), while females have egg-restricted stem cells (EgSCs). These GSCs self-renew in a polyp, and are usually transmitted to a new bud from a parental polyp during asexual reproduction. But if these GSCs are lost during subsequent budding or regeneration events, new ones are generated from multipotent stem cells (MPSCs). MPSCs are the somatic stem cells in Hydra that ordinarily differentiate into nerve cells, nematocytes (stinging cells in cnidarians), and gland cells. By means of such a backup system, sexual reproduction is guaranteed for every polyp. Interestingly, Hydra polyps occasionally undergo sex-reversal. This implies that each polyp can produce either type of GSCs, i.e. Hydra are genetically hermaphroditic. Nevertheless a polyp possesses only one type of GSCs at a time. We propose a plausible model for sex-reversal in Hydra. We also discuss so-called germline specific genes, which are expressed in both GSCs and MPSCs, and some future plans to investigate Hydra GSCs.     

Taxonomy of the European Hydra (Cnidaria: Hydrozoa): a re-examination of its history with emphasis on the species H. vulgaris Pallas,H. attenuata Pallas and H. circumcincta Schulze

The name Hydra attenuata Pallas is currently applied to the wrong animal. The common brown polyp, which is widely called H. attenuata, was described by Pallas (1766) as Hydra vulgaris. The name H. attenuata Pallas originally referred to an uncommon pale polyp, currently known as H. circumcincta Schulze. The history of this confusion is analysed here. The taxonomy of hydra was in disarray during the 18th and 19th centuries, and was clarified in 1917 with the monograph of Schulze. But Schulze misapplied the name for the common hydra, H. vulgaris, to an unusual form and thus was led to assign the name of a rare hydra, H. attenuata, to the common type. Schulze redescribed the rare, pale hydra that Pallas had named H. attenuata as H. circumcincta. The correct name of the common European brown, stalkless hydra is thus H. vulgaris Pallas, 1766. The name H. attenuata has priority for the uncommon pale hydra, but because of disuse of this application of the name, the pale hydra should be recognized by the current, generally accepted binominal H. circumcincta Schulze, 1914.     

Identification and characterization of the epithelial polarity receptor ”Frizzled” in Hydra vulgaris

The Wnt signaling pathway plays an important role in the specification of cell patterning during development in many species. Here we report the isolation and characterization of a putative Wnt receptor, Frizzled, in Hydra vulgaris. Analysis of the amino acid sequence of Frizzled in hydra reveals that this receptor contains many strong sequence similarities to other known Frizzled receptors. Hydra divergence is estimated to have occurred about one billion years ago; thus comparison of the Frizzled sequence of hydra with that of other species is likely to provide important information on the structure and function of those highly conserved regions. Northern and Southern blotting reveal that the Frizzled receptor in hydra has a 2.34-kb message size, and that it is encoded by a single gene. In situ hybridization using hydra frizzled as a probe reveals that the receptor message is restricted to the endoderm in adult hydra. This distribution supports the hypothesis that the Frizzled receptor is functioning in a pathway that controls cell differentiation in hydra. Received: 24 September 1999 / Accepted: 7 December 1999