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1.
Guidong Miao Changwei Shao Hongyu Ma Xiaolin Liao Songlin Chen 《Conservation Genetics》2009,10(3):631-633
Starry flounder (Platichthys stellatus) is a rare fish species in China. Here, we reported 12 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of starry flounder (P. stellatus). The number of alleles, observed and expected heterozygosity per locus in 30 individuals ranged from two to six, from 0.2500
to 1.0000 and from 0.4512 to 0.7667, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species amplification
of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in P. stellatus.
Guidong Miao and Changwei Shao have contributed equally. 相似文献
2.
Yong-Sheng Tian Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Song-Lin Chen 《Conservation Genetics》2009,10(4):1041-1043
Stone flounder (Kareius bicoloratus) is a rare fish species in China. Here, we reported 11 polymorphic microsatellite loci isolated from a repeat-enriched genomic
library of stone flounder. The number of alleles, observed and expected heterozygosity per locus in 32 individuals ranged
from 3 to 6, from 0.1613 to 0.8667 and from 0.1549 to 0.7932, respectively. Two loci significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction and no significant linkage disequilibrium between pairs of loci was found. Cross-species
amplification of these microsatellite loci in additional three fish species was performed. These polymorphic microsatellite
loci would be useful for investigating genetic population structure and construction of genetic linkage map in Kareius bicoloratus.
Yong-Sheng Tian and Gui-Dong Miao are contributed equally. 相似文献
3.
Gui-Dong Miao Chang-Wei Shao Xiao-Lin Liao Hong-Yu Ma Yong-Sheng Tian Song-Lin Chen 《Conservation Genetics》2009,10(3):701-703
Barfin flounder (Verasper moseri) is a rare fish species in the world. Here, we reported 10 polymorphic microsatellite loci isolated from a dinucleotide-enriched
genomic library of barfin flounder (Verasper moseri). The number of alleles, observed, and expected heterozygosity per locus in a test population ranged from 2 to 6, from 0.3333
to 1.0000, and from 0.4866 to 0.7774, respectively. One locus significantly deviated from Hardy–Weinberg equilibrium after
Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification
of these microsatellite loci in additional five fish species was performed. These polymorphic microsatellite loci would be
useful for investigating genetic population structure and construction of genetic linkage map in Verasper moseri.
Gui-Dong Miao and Chang-Wei Shao Contributed equally to this work. 相似文献
4.
Seven-band grouper (Epinephelus septemfasciatus) is a commercially important fishery species. Sixty-six microsatellite loci were isolated from a dinucleotide-enriched genomic
library of E. septemfasciatus. Twelve of these loci were polymorphic in a test population with alleles per locus ranging from two to five, and observed
and expected heterozygosities per locus from 0.04 to 1.00 and from 0.28 to 0.76, respectively. Three loci significantly deviated
from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci after Bonferroni
correction. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of E. septemfasciatus and other related species.
Lili Zhao and Changwei Shao have contributed equally. 相似文献
5.
Song-Lin Chen Lili Zhao Chang-Wei Shao Xiaolin Liao Yong-Sheng Tian 《Conservation Genetics》2009,10(4):1101-1103
Black sea bass (Centropristis striata) is an economically important serranid species. A number of 39 microsatellite loci were isolated from two enriched genomic
library of C. striata. Eleven of these loci were polymorphic in a test population with alleles per locus ranging from 3 to 8, and observed and
expected heterozygosities per locus from 0.26 to 1.00 and from 0.61 to 0.84, respectively. Four loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species.
These polymorphic microsatellite loci would be useful for investigating genetic resource of C. striata and other related species. 相似文献
6.
Lili Zhao Changwei Shao Xiaolin Liao Hongyu Ma Xuechun Zhu Songlin Chen 《Conservation Genetics》2009,10(3):743-745
Yellow grouper (Epinephelus awoara) is a commercially important marine fish species. A dinucleotide-enriched genomic library of E. awoara was constructed using the method of FIASCO. Twelve loci were polymorphic in a test population with alleles per locus ranging
from two to eight, and observed and expected heterozygosities per locus from 0.13 to 1.00 and from 0.20 to 0.86, respectively.
Five loci significantly deviated from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found between
pairs of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related
species. These polymorphic microsatellite loci would be useful for investigating genetic diversity of E. awoara and related species.
L. Zhao and C. Shao have contributed equally to this work. 相似文献
7.
Song-Lin Chen Shi-Chao Xing Gen-Bo Xu Xiaolin Liao Jing-Feng Yang 《Conservation Genetics》2009,10(5):1469-1471
Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library
of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs
of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis. 相似文献
8.
J. A. Galarza S. Roques J. Carreras-Carbonell E. Macpherson G. F. Turner C. Rico 《Conservation Genetics》2007,8(5):1251-1253
Eight polymorphic microsatellite loci were isolated and characterized for the Cardinal fish (Apogon imberbis), a coastal-reef fish endemic to the Mediterranean Sea. Characterization of 30 Cardinal fish individuals form the western
Mediterranean showed moderate to high allelic diversity ranging from 6 to 19 alleles per locus. Two loci showed significant
departures from Hardy-Weinberg equilibrium presumably due to null alleles. No evidence of linkage disequilibrium was found
for any locus pairwise comparasions. This microsatellite set could be useful for any basic population genetic studies of this
species. 相似文献
9.
Isolation and characterization of polymorphic microsatellite loci from fat greenling (Hexagrammos otakii) 总被引:2,自引:0,他引:2
Song-Lin Chen Gui-Dong Miao Chang-Wei Shao Yong-Sheng Tian Xiaolin Liao 《Conservation Genetics》2009,10(5):1429-1431
Fat greenling (Hexagrammos otakii) is an economocally important marine fish species. Thirtyfive microsatellite loci were isolated from two enriched genomic
library of Hexagrammos otakii. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and
expected heterozygosities per locus from 0.2581 to 1.0000 and from 0.2892 to 0.7726, respectively. Four loci significantly
deviated from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between
pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Hexagrammos otakii. 相似文献
10.
Shi-Chao Xing Gen-Bo Xu Xiao-Lin Liao Guan-Pin Yang Song-Lin Chen 《Conservation Genetics》2009,10(4):1167-1169
In the study, 34 microsatellite loci were isolated from a dinucleotide-enriched genomic library of Japanese Spanish mackerel
(Scomberomorus niphonius). And 12 microsatellite loci were found to be polymorphic between 3 and 8 alleles. The number of observed and expected heterozygosity
per locus in 23 individuals ranged from 0.6087 to 1.0000 and 0.8908 to 0.9773, respectively. Two loci significantly deviated
from Hardy–Weinberg equilibrium after Bonferroni correction analysis and there was no significant linkage disequilibrium found
between pairs of loci. As a result, 12 microsatellite loci probably located on different chromosome pairs and these polymorphic
microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure
and evaluate the breeding strategy in S. niphonius.
The authors Shi-Chao Xing and Gen-Bo Xu contributed equally. 相似文献