Fungal Olecranon Bursitis in an Immunocompetent Patient by <Emphasis Type="Italic">Knoxdaviesia dimorphospora</Emphasis> sp. nov.: Case Report and Review

Bursitis is a common medical condition that can occur either with or without infection. We present a case of fungal olecranon bursitis in an immunocompetent individual caused by the new species Knoxdaviesia dimorphospora. It is a dematiaceous filamentous fungus characterized by the production of two different conidia: hyaline and cylindrical, which rise up from phialidic conidiogenous cells located in the upper part of differentiated and unbranched conidiophores, and pale brown and ellipsoidal conidia produced by phialidic conidiogenous cells which are born directly on hyphae. In addition to its morphological peculiarities, the novelty of the fungus was confirmed by sequence analysis of the internal transcribed spacer (ITS) regions and D1/D2 domains of the 28S of the nuclear rRNA gene. The fungal infection was confirmed by cytological examination and repeated cultures. The infection was resolved by surgical debridement and drainage, and the patient presented a complete functional recovery 3 months later. The in vitro antifungal susceptibility to this new human opportunist is provided, terbinafine being the drug with the most potent activity.     

Taxonomy and Phylogeny of Synchaetomella Lunatospora, a New Genus and Species of Synnematous Fungi from Southeast Asia

The anamorphic fungus Synchaetomella lunatospora gen. et sp. nov. is described from a culture isolated from leaf litter in Singapore. The genus is characterized by the production of synnematous conidiomata with brown stipes, phialidic conidiogenesis with terminal and acropleurogenous conidiogenous cells, and falcate, 1-septate conidia, aggregating in white, mucilaginous heads. Its phylogenetic relationships, inferred from the partial nuclear small ribosomal subunit (18S) sequence, are with the coelomycetous genera Sphaerographium, Chaetomella, and Hainesia. However, the order and family affinities of this group are unclear.     

FENESTROCONIDIA CALOPLACAE,A NEW SPORODOCHIAL LICHENICOLOUS FUNGUS WITH CATENATE CONIDIOGENOUS CELLS

The genus Fenestroconidia is described to accommodate the new species Fenestroconidia caloplacae. This lichenicolous fungus produces black sporodochia, has conidiogenous cells arranged in chains, and simple, pale brown conidia with a subhyaline guttule-like zone. It is known only from central Spain (Cuenca province), growing in the apothecia of Caloplaca saxicola s.lat.     

<Emphasis Type="Italic">Cylindrosporella</Emphasis> on betulaceous trees in Japan

Three Cylindrosporella species on the leaves of betulaceous trees – C. carpini, C. coryli, and C. microsperma – were first reported from Japan. The genus Cylindrosporella is sometimes treated as congeneric with Asteroma; however, we considered these to differ based on conidial morphology following the concept of Arx. The genus Cylindrosporella is characterized by one-celled, filiform or fusiform, small conidia that are often curved, have hyaline, thin walls, and are produced from phialidic conidiogenous cells in subcuticular, fiat acervuli. The three species are distinguished from each other on the basis of conidium size. Received: September 27, 2001 / Accepted: November 5, 2001     

Conidial development in selected ambrosial species of the genus <Emphasis Type="Italic">Raffaelea</Emphasis>

Conidiogenesis of the type species and two additional species of the genus Raffaelea Arx & Hennebert are described. In contrast to previous conclusions based on light microscopy that indicate sympodial production of the conidia, we found that conidium development was by annellidic percurrent proliferation in the type species Raffaelea ambrosiae, as well as in Raffaelea arxii and Raffaelea albimanens. Consequently, this mode of conidium development is similar to the conidiogenesis of anamorphs within the Ophiostomatales, but distinguished by sporodochia formed within the genus Raffaelea. These findings reduce the differences between Raffaelea and other anamorphs with annellidic percurrent proliferation of the conidiogenous cells within the Ophiostomatales to different conidiomata and conidiophores, and they support previous reports of the close phylogenetic relationship between Raffaelea and Ophiostoma.     

小麦雪霉叶枯病菌侵染过程的细胞学研究

采用电镜技术研究了小麦雪霉叶枯病菌(Gerlachia nivalis)侵染过程的细胞学特征。电镜观察发现,分生孢子萌发产生的芽管由孢子细胞壁内层延伸而成;病菌侵入寄主体内后,胞间菌丝先在寄主细胞间扩展,随后胞间菌丝侵入坏死的寄主细胞,形成胞内菌丝;胞间菌丝和胞内菌丝在形态结构上无明显差异。在病菌扩展过程中,寄主细胞发生了一系列的病理变化,并最终坏死消解,寄主细胞的变化可能与病菌分泌的毒素有关。     

Phylogeny and morphology of Hirsutella tunicata sp. nov. (Ophiocordycipitaceae), a novel mite parasite from Peru

A new species of Hirsutella was isolated from unidentified mites on Petri plates inoculated with soil and root fragments collected from asparagus rhizosphere at Virú, Northern Peru. The fungus differs from other Hirsutella species by an envelope surrounding the conidium, conidia dimension and DNA sequences. In PDA cultures, the mycelium produced aerial hyphae with conidiogenous cells mainly at right angles, occasionally showing a secondary conidiophore. The solitary conidia are cymbiform, slightly apiculate, 5.0–6.0 × 3.0–4.0 μm. Phylogenetic analyses with partial rRNA and β-tubulin gene sequences confirmed the fungus as an Hirsutella (Ophiocordycipitaceae). Closest species shown by maximum likelihood and neighbor-joining trees were H. nodulosa and H. aphidis, from which the new species differs for conidium or conidiogenous cells dimensions, lack of synnemata and host type. A recombination event was also detected in the rRNA of the holotype strain, involving Ophiocordyceps sinensis as major parent and O. cochlidiicola as minor parent. A complement, inverted insertion was also found in its rRNA, involving part of the ITS2 and 5.8S regions, flanked by two short nucleotide arrays. Due to conidia dimension and phylogenetic position, the fungus is described as Hirsutella tunicata sp. nov. A review of mononematous Hirsutella species is provided.     

Description ofAvesicladiella gen. nov. (hyphomycetes) for two undescribed leaf litter microfungi

The new hyphomycete genusAvesicladiella with two species,A. britannica sp. nov., type species, andA. microsperma sp. nov., is described and illustrated. Both species are leaf litter microfungi. The genus closely resemblesVesicladiella, Circinotrichum, Gyrothrix, Ceratocladium andVermiculariopsiella, several hyphomycete genera with comparable characters such as superficial hyphal networks; production of setae; absence of conidiophores; lageniform or ampulliform conidiogenous cells; and hyaline aseptate conidia produced in clusters around the apices of conidiogenous cells. The new genus differs from these by its hyaline, septate setae, typical ‘phialidic’ conidiogenous cells, and hyaline, aseptate cylindrical conidia.     

CODONMYCES AND LICHENOSTELLA,TWO NEW GENERA OF LICHENICOLOUS CONIDIAL FUNGI

The new generic names Codonmyces and Lichenostella are introduced for two new species of conidial fungi. Lichenostella hypotrachynae gen. et sp. nov. is a commensal of Hypotrachyna species, and is mainly characterized by forming black sporodochia with setae and, particularly, by its star-like conidia. Codonmyces lecanorae gen. et. sp. nov. occurs on Lecanora valesiaca and differs from the genus Xanthoriicola by its 1-septate conidia and campanulate conidiogenous cells with several conspicuous, flared annellations. Comments on the ecology ofL. valesiaca , and its associated lichens and lichenicolous fungi are also given.     

CONIDIOGENESIS IN CERATOCYSTIS ULMI,CERATOCYSTIS PICEAE,AND GRAPHIUM PENICILLIOIDES

Conidiogenous cells of both the synnematous and mononematous conidiophores in Ceratocystis ulmi and C. piceae develop by sympodial proliferation. Holoblastic conidia are produced on nodules or short denticles from the synnematous conidiogenous cells and on well-defined denticles from the mononematous conidiogenous cells. Graphium penicillioides is characterized by percurrent proliferation of the conidiogenous cells and the conidia are holoblastic and annellidic. A comparison of the type material of G. penicillioides with the lectotype specimen of C. piceae indicates that G. penicillioides is not the conidial state of C. piceae. The method of conidial development in C. ulmi and C. piceae is distinct from that of G. penicillioides, the lectotype species of Graphium; these conidial states are, therefore, placed in a new hyphomycete genus, Pesotum, as the Pesotum state of Ceratocystis ulmi and C. piceae, respectively.     

Phaeomoniella zymoides and Phaeomoniella pinifoliorum spp. nov., new acid-tolerant epiphytic fungi isolated from pine needles in Korea

Two new epiphytes of anamorphic ascomycetes, Phaeomoniella zymoides sp. nov. and Phaeomoniella pinifoliorum sp. nov., were isolated from the needle surface of Pinus densiflora in Korea. The new taxa were characterized by acid-tolerant, slow, partially yeast-like growth and extensive production of emerging cells on convex wrinkled mycelial colonies. Phaeomoniella zymoides produced mycelium with large numbers of intercalary and lateral or terminal vesicles or swollen cells. Large conidiogenous cells had a swollen base and appeared to be phialidic, and many phialoconidia also were produced from lateral hyphal apertures. Maturing colonies of Ph. zymoides were made up of dark green to blackish areas and produced a Phoma-like synanamorph. Primary conidia became elongate mother cells giving rise to polar or lateral secondary conidia. Phaeomoniella pinifoliorum was characterized by reduced, swollen, phialide-like cells, lateral production of conidia from hyphae and terminal or subterminal, or less commonly lateral, secondary production of conidia from yeast-like primary conidia. When ITS and 28S rDNA sequences were compared and analyzed with those of best matching GenBank taxa, the Phaeomoniella group consisted of three lineages, "zymoides", "pinifoliorum" and "chlamydospora" clades, which again showed a complete sister relationship to Moristroma quercinum ined.     

The genus <Emphasis Type="Italic">Fusariella</Emphasis>

The genus Fusariella, typified by F. atrovirens, is characterised by semi- to macronematous, mononematous conidiophores, with cylindrical, subulate or lageniform phialidic conidiogenous cells that produce catenate, septate, curved to straight, subhyaline to brown conidia. During a survey of hyaline-spored hyphomycetes from karst areas in Thailand, we collected a new species of Fusariella with curved conidia and introduce it in this paper as Fusariella curvata sp. nov. In addition, all hitherto described species of Fusariella are reviewed. The result of phylogenetic analyses, based on combined SSU, LSU, TEF and RPB2 sequence data, indicates that the genus belongs in the family Bionectriaceae (Hypocreales, Sordariomycetes).     

<Emphasis Type="Italic">Sativumoides</Emphasis> and <Emphasis Type="Italic">Cladosporiopsis</Emphasis>, two new genera of hyphomycetes from China

Sativumoides punicae gen. et sp. nov. and Cladosporiopsis ovata gen. et sp. nov. are described and illustrated. Sativumoides is characterized by macronematous, individual, percurrently extending conidiophores, integrated, monoblastic, apical, lageniform conidiogenous cells, and single, apical, pale brown to brown, garlic-bulb-shaped dictyosporous conidia with predominantly longitudinal septa. Cladosporiopsis is unique in possessing pigmented, apiosporous conidia seceding schizolytically from integrated, monoblastic, determinate conidiogenous cells; the conidia, arising from the sides or ends of ramoconidia, form short, acropetal chains. Sativumoides is compared with similar anamorphic genera including Triposporium, Iyengarina, Acrodictys, Actinocladium, Piricaudiopsis, Cheiroidea, Stephanoma, Domingoella, Acrogenospora and Shrungabeeja. The distinctions between Cladosporiopsis and similar genera including Cladosporium, Ochrocladosporium, Rachicladosporium, Rhizacladosporium, Toxicocladosporium, Ramularia, Bispora, Lylea and Devriesia are also discussed. Keys to these two novel genera and their morphologically similar genera are provided.     

Kabatiella bupleuri sp. nov. (Dothideales), a pleomorphic epiphyte and endophyte of the Mediterranean plant Bupleurum gibraltarium (Apiaceae)

Bupleurum gibraltarium is an evergreen shrub endemic to southern Spain and northern Algeria and Morocco. We have collected and cultured an undescribed Kabatiella species that is consistently associated with the flower rachises and leaves of B. gibraltarium in the province of Granada. The fungus forms melanized acervuli on overwintered flower rachises. It also can be isolated from yeast-like conidial masses that emerge from senescing leaves and from surface-disinfected healthy leaves. Like other Kabatiella species, the fungus forms blastic falcate to lunate conidia simultaneously from the apex of conidiogenous cells in acervuli. In culture, melanized single-septate conidia form blastically from the vegetative hyphae that accumulate in yeast-like masses. These conidia germinate by budding to form secondary yeast-like cells or directly as hyphae. In culture, the fungus behaves like, and could be confused with, Aureobasidium and Hormonema species. We describe the growth of this species in agar media and its phylogenetic position based on the analyses of nuclear ribosomal RNA gene sequences. This new species is a sister species of the morphologically similar clover pathogen, K. caulivora.     

Polyphasic characterization of Plectosphaerella oligotrophica,a new oligotrophic species from China

Oligotrophic fungi can grow on carbon-free media. Most of the currently known oligotrophic fungi were reported from soil. Plectosphaerella oligotrophica sp. nov. was recently isolated from soil in China using a low carbon medium. The new species is morphologically characterized by pale yellow colonies on PDA, phialidic conidiogenous cells, and hyaline, ellipsoidal and 0–1-septate conidia. Phylogenetic analysis based on internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequences confirmed the placement of P. oligotrophica as a new species in the genus Plectosphaerella. The new species is compared to the morphologically and phylogenetically closely related species. Beta-tubulin fragments from the new species were also sequenced and deposited in GenBank. A key to the currently accepted species of the genus is provided.     

Fusarium kyushuense sp. nov. from Japan

Four trichothecene-producing strains originally isolated from diseased wheat and a vinyl plate in Kyushu and Shikoku, Japan are described and illustrated as a new species,Fusarium kyushuense. This species does not produce chalamydospores, which is the key morphological character which distinguishes it fromF. sporotrichioides with which it has been mistaken.Fusarium kyushuense is also differentiated from another morphologically similar species,F. arthrosporioides, by absence of sclerotia and by differences in conidiogenesis of obovate, conidia. InF. arthrosporioides conidia are partly holoblastic from the aerial conidiophores and mostly phialidic from the sporodochial conidiophores, while inF. kyushuense conidia are mostly holoblastic and only produced from aerial conidiophores.     

分离自蜻蜒虫草的拟青霉属一新种

本文描述了拟青霉属(Paecilomyces)一新种—蜻蜓拟青霉(P. odonatae),该菌分离自蜻蜓虫草(Cordyceps odonatae),与其它种的典型区别特征是它具有拟青霉型产孢结构,产椭圆形链状排列的分生孢子和枝顶孢霉型产孢结构,产柱状粘成团排列的分生孢子.     

Three new species and a new record of Diplococcium from plant debris in Spain

Diplococcium dimorphosporum sp. nov., D. racemosum sp. nov., D. singulare sp. nov. and D. pulneyense Subram. & Sekar collected from plant debris in natural areas of Spain are described and illustrated. The first species is characterized principally by the production of branched conidiophores and short chains of conidia. Diplococcium singulare has unbranched conidiophores, and conidia produced usually at the tip of conidiophores and from lateral spherical conidiogenous cells. In addition, both species develop a Selenosporella synanamorph with narrow falcate conidia. Diplococcium racemosum produces branched, verrucose conidiophores, and verrucose conidia in long branched chains. Diplococcium pulneyense is the second record, being described for first time on the natural substratum and re-described in pure culture. A key to currently accepted species of Diplococcium is provided.     

Minimelanolocus bicolorata sp. nov., Paradendryphiopsis elegans sp. nov. and Corynesporella bannaense sp. nov. from southern China

Minimelanolocus bicolorata sp. nov., Paradendryphiopsis elegans sp. nov. and Corynesporella bannaense sp. nov., are described and illustrated. Minimelanolocus bicolorata is unique in possessing the schizolytic conidial secession and solitary, acropleurogenous, holoblastic conidia that are ellipsoidal, 3-euseptate, 30–35 × 7.5–9.5 μm, verruculose, pale brown, with an appendage at each end. Paradendryphiopsis elegans is characterized by monoblastic conidiogenous cells producing short chains of conidia that are 13–30.5 × 4–6.5 μm, 2–3-euseptate and fusiform to obclavate. Corynesporella bannaense is distinguished by terminal and integrated or discrete, monotretic conidiogenous cells that arise as lateral branches from the tip of the conidiophore and solitary conidia that are 12–16-distoseptate, smooth, obclavate, thick-walled, pale brown, and 100–140 × 10–14 μm. Keys to species of Minimelanolocus, Paradendryphiopsis and Corynesporella are provided.     

Production ofbeauveria bassiana [Deuteromycotina. Hyphomycetes] sympoduloconidia in submerged culture

Submerged conidiation of the entomogenous HyphomyceteBeauveria bassiana is reported. Conidiogenous cells produce sympoduloconidia on conidiogenous cells in liquid shaker culture; hyphal bodies and mycelium fragments are also produced. The morphology of these fungal structures is discussed and illustrated. Several simple liquid media are tested for the production of conidia and hyphal bodies. Maximum yields of conidia (170×10⁶ conidia/ml) are produced in a medium consisting of sucrose (2%) — yeast extract (0.5%) and basal salts, and maximum yields of hyphal bodies (740×10⁶ hyphal bodies/ml) in a sucrose (2.5%) — yeast extract (2.5%) medium.