共查询到20条相似文献,搜索用时 15 毫秒
1.
Prashali Bansal Johannes Madlung Kristina Schaaf Boris Macek Fulvia Bono 《Molecular & cellular proteomics : MCP》2020,19(9):1485-1502
Highlights
- •Label-free and dimethyl labeling MS analysis of 6 RBPs from Drosophila ovaries.
- •Functionally related RBPs show overlapping proteomes.
- •Selective co-purification of splicing factors and translational regulators.
- •Validation of 26 novel interactions by co-immunoprecipitation.
2.
《Molecular & cellular proteomics : MCP》2020,19(3):456-466
Highlights
- •Urinary proteomes of patients with recurrent UTI, renal scarring, and VUR.
- •80 proteins differentially expressed, compared to healthy controls.
- •62 proteins may be indicative of susceptibility for UTI.
- •Altered acute phase response, extracellular matrix and carbohydrate metabolism.
3.
《Molecular & cellular proteomics : MCP》2020,19(11):1910-1920
Highlights
- •PRMT5 glutathionylation is increased in aged mice or under oxidative stress.
- •Deglutathionylation of PRMT5 is catalyzed by glutaredoxin-1.
- •PRMT5 glutathionylation decreases its methyltransferase activity.
- •PRMT5 glutathionylation results in G2/M arrest and inhibits cell proliferation.
4.
Tirsa L. E. van Westering Henrik J. Johansson Britt Hanson Anna M. L. Coenen-Stass Yulia Lomonosova Jun Tanihata Norio Motohashi Toshifumi Yokota Shin'ichi Takeda Janne Lehti Matthew J. A. Wood Samir EL Andaloussi Yoshitsugu Aoki Thomas C. Roberts 《Molecular & cellular proteomics : MCP》2020,19(12):2047-2068
Highlights
- •Proteomics analysis was performed in two murine models of Duchenne muscular dystrophy (mdx and mdx52) at three ages (8, 16 and 80 weeks) and compared with wild-type controls.
- •High-resolution isoelectric focusing liquid chromatography-tandem mass spectrometry enabled the quantification of 4974 proteins in all samples.
- •This study has revealed protein signatures of dystrophin deficiency and the progression of dystrophic pathology.
- •In contrast, the proteomes of the mdx and mdx52 mice were highly similar.
- •Pathway analysis revealed crosstalk between inflammatory, metabolic and muscle growth processes in dystrophic muscle.
5.
《Molecular & cellular proteomics : MCP》2020,19(6):928-943
Highlights
- •EGFR-TKI molecular response profiling covering 10138 proteins and 13486 mRNAs.
- •EGFR-TKI combination therapy screen using a library of 528 compounds.
- •Several new candidate EGFR-TKI escape mechanisms and combination therapy targets.
- •Combined targeting of the oncogene BCL6 and EGFR results in synergy in NSCLC cells.
6.
《Molecular & cellular proteomics : MCP》2020,19(7):1209-1219
Highlights
- •In depth performance assessment of leading tools for differential protein abundance.
- •Novel fast modular framework MSqRobSum for robust protein summarization and inference.
- •MsqRobSum outperforms leading protein summarization-based tools.
- •MSqRobSum is on par with top-performing peptide based tool MSqRob.
7.
《Molecular & cellular proteomics : MCP》2020,19(1):181-197
Highlights
- •Provision of data generated on the basis of a gold standard spike-in sample set.
- •Choice of spectral library has great impact on identification and quantification.
- •DIA is superior to DDA in quantification reproducibility, specificity and accuracy.
- •DIA outperforms DDA in the quantification of low protein amounts.
- •Quantification on peptide level is generally preferable.
8.
《Molecular & cellular proteomics : MCP》2020,19(4):574-588
Highlights
- •SILAC-based protein quantification of OA hBMSCs undergoing chondrogenesis.
- •Spatially-resolved metabolomics by MSI of hBMSCs in chondrogenic differentiation.
- •Differential metabolic pathways involved in OA compared to control hBMSCs.
- •UDP-glucuronic acid/UDP-GlcNAc synthesis is decreased in chondrogenic OA hBMSCs.
9.
《Molecular & cellular proteomics : MCP》2020,19(6):1005-1016
Highlights
- •Brain membrane protein extraction.
- •Protein prenylation.
- •Prenyl peptide capture and characterization by LC-MS/MS.
- •HCD and EThcD peptide fragmentation.
10.
Nataly Mancette Rijensky Netta R. Blondheim Shraga Eilon Barnea Nir Peled Eli Rosenbaum Aron Popovtzer Solomon M. Stemmer Alejandro Livoff Mark Shlapobersky Neta Moskovits Dafna Perry Eitan Rubin Itzhak Haviv Arie Admon 《Molecular & cellular proteomics : MCP》2020,19(8):1360-1374
Highlights
- •Sufficient tumor tissues are often unavailable large HLA peptidome discovery.
- •Using patient derived xenograft (PDX) tumors can overcome this limitation.
- •The large PDX HLA peptidomes expand significantly those of the original biopsies.
- •The HLA peptidomes of the PDX tumors included many tumor antigens.
11.
《Molecular & cellular proteomics : MCP》2020,19(4):690-700
Highlights
- •Two molecular groups in anal squamous carcinoma according proteomic profile.
- •Differences in possible targeted processes such as metabolism or immune response.
- •Different percentage of tumor lymphocyte infiltration.
- •Difference in the frequency of ATM variants, related to PPAR inhibitors.
12.
《Molecular & cellular proteomics : MCP》2020,19(6):1058-1069
Highlights
- •Highly parallelizable 4D feature detection in ion mobility enhanced shotgun proteomics.
- •Multidimensional non-linear mass, retention time and ion mobility recalibration.
- •Collision cross section aware matching between runs.
- •Label-free quantification of ion mobility MS data.
13.
《Molecular & cellular proteomics : MCP》2020,19(6):916-927
Highlights
- •Summarize the development of functional protein microarray.
- •Application of functional proteome microarray in basic research.
- •Application of functional proteome microarray in translational research.
- •Fabrication of functional membrane protein array using virion display method.
14.
《Molecular & cellular proteomics : MCP》2020,19(1):114-127
Highlights
- •Quantitative proteomics and machine learning to study plasma biomarkers in HCM.
- •Six peptides are increased in plasma of LVH+ HCM compared to controls.
- •Peptide biomarkers correlate with imaging markers of phenotype severity.
- •Peptide biomarkers correlate with the estimated sudden cardiac death risk.
15.
《Molecular & cellular proteomics : MCP》2020,19(3):478-489
Highlights
- •Comprehensive molecular profiling of cutaneous and cerebellar metastasis variants.
- •Identification of differentially regulated metastasis-associated molecules.
- •Evidence for individually distinct patterns of metastasis-associated molecules.
- •Highlighting the evident need for establishing meta-analyses strategies.
16.
Yi-Han Lin Maryann P. Platt Haiyan Fu Yuan Gui Yanlin Wang Norberto Gonzalez-Juarbe Dong Zhou Yanbao Yu 《Molecular & cellular proteomics : MCP》2020,19(12):2030-2047
Highlights
- •Cecal Ligation Puncture (CLP) mouse model to study sepsis-induced kidney disease.
- •Quantitative global proteome and phosphoproteome profiling of mouse kidneys.
- •Highly significant candidate markers for onset and progression of AKI to CKD.
- •Mechanistic insights into sepsis-associated kidney injuries.
17.
《Molecular & cellular proteomics : MCP》2020,19(5):744-756
Highlights
- •Signaling networks can be highly heterogeneous across cells in a tissue.
- •Various technologies allow analyzing signaling networks at single-cell resolution.
- •The advantages and limitations of each single-cell approach are summarized.
- •Confounding factors in single-cell signaling network analysis are discussed.
18.
Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons
Charlotte A. G. H. van Gelder Renske Penning Tim S. Veth Lisa A. E. Catsburg Casper C. Hoogenraad Harold D. MacGillavry Maarten Altelaar 《Molecular & cellular proteomics : MCP》2020,19(12):1952-1968
Highlights
- •Integrated phosphoproteomics and analyses of newly synthesized proteins in neurons.
- •Resource of temporal mGluR-induced signaling pathways upon DHPG stimulation.
- •Validation of PKC, MAPK1, CAMKIIa, and CDK2 in mGluR-activation and signaling.
- •Validation of Intersectin-1 in DHPG-induced AMPAR internalization.
19.
《Molecular & cellular proteomics : MCP》2020,19(4):624-639
Highlights
- •Used affinity-enrichable, isotopically coded, and MS-cleavable crosslinker.
- •Targeted acquisition strategy based on isotopic-coding described and evaluated.
- •Novel data analysis pipeline developed provides improved crosslink identification.
- •Large dataset reveals hundreds of mitochondrial protein-protein interactions.
20.
《Molecular & cellular proteomics : MCP》2020,19(2):308-325
Highlights
- •Multi-omics analysis on mode of action of novel antimalarial, JPC-3210
- •JPC-3210 has rapid parasite killing kinetics.
- •Metabolomics and peptidomics demonstrated JPC-3210 inhibits hemoglobin digestion.
- •Proteomics demonstrated JPC-3210 enriches for translation regulation proteins.