HIV－1反式激活因子（Tat）作用机理的研究进展

ＨＩＶ－１反式激活因子（Ｔａｔ）作用机理的研究进展梁臣,耿运琪（南开大学生命科学学院,天津３０００７１）关键词ＨＩＶ－１,Ｔａｔ,ＴＡＲ人免疫缺陷病毒－１（ＨＩＶ－１）属反转录病毒科,慢病毒亚科,为艾滋病的重要病原体。该病毒可长期潜伏于人体内,但在某...     

反式激活HIV—1LTR的人疱疹病毒6型（HHV—6）基因片段的初步研究

艾滋病（ＡＩＤＳ）主要是由人免疫缺陷病毒（ＨＩＶ）侵入人体后,破坏人的免疫系统造成的。许多流行病学研究已证明,疱疹病毒与ＨＩＶ的共感染可以导致对ＨＩＶ－１启动子的激活,并加速细胞的病理性反应〔１〕,从而加大个体对ＨＩＶ感染的敏感和加快疾病的进程。人疱...     

从HIV－Ⅰ中新发现编码含硒蛋白的基因探讨艾滋病化疗新方法

从ＨＩＶ－Ⅰ中新发现编码含硒蛋白的基因探讨艾滋病化疗新方法刘琼,徐辉碧（华中理工大学化学系,武汉４３００７４）关键词　艾滋病,人类免疫缺陷病毒Ⅰ型,硒艾滋病是一种目前尚无有效防治方法的疾病。它是由人类免疫缺陷病毒（ＨＩＶ）感染,并在宿主细胞内大量复制...     

广东两株人免疫缺陷病毒的分离和鉴定

从我省两名经性途径感染艾滋病的病人中采血,分离外周血单核细胞（ＰＢＭＣｓ）,将其与正常人ＰＢＭＣｓ共培养分离人免疫缺陷病毒（ＨＩＶ）,３周后检测上清ＨＩＶ－１ｐ２４抗原（ＥＬＩＳＡ法）超过阈值。将共培养第四周细胞和上清分别感染Ｈ９细胞（Ｔ细胞淋巴瘤传代细胞）,一周后检测ＨＩＶ－１ ｐ２４怕,证明有病毒生长。用ＨＩＶ－１ ＥＮＶ基因引物的套式聚合酶链反应（Ｎｅｓｔｅｄ ＰＣＲ）证实,两株新分离的病毒     

人免疫缺陷病毒HIV-1 gag和env基因片段在大肠杆菌中的表达

人免疫缺陷病毒ＨＩＶ－１ｇａｇ和ｅｎｖ基因片段在大肠杆菌中的表达祁自柏,谷金莲,李河民（中国药品生物制品检定所,北京１０００５０）由于引起艾滋病的ＨＩＶ－１病毒的多变性,使得艾滋病的预防和治疗遇到很大困难。我们从一株用艾滋病人外周血淋巴细胞感染的细胞...     

HIV－1感染初始阶段的分子机制

ＨＩＶ－１感染初始阶段的分子机制项秉懿（第四军医大学分子生物学研究所,西安７１００３２）关键词艾滋病,人类免疫缺陷病毒－Ｉ型,包膜糖蛋白ｇｐ１２０,ＣＤ４受体,感染ＴｈｅＭｏｌｅｃｕｌａｒＭｅｃｈａｎｉｓｍｏｆＨＩＶ－１ＩｎｆｅｃｔｉｏｎｉｎｔｈｅＩ...     

人类免疫缺陷病毒细胞辅助受体的研究进展

人类免疫缺陷病毒细胞辅助受体的研究进展谭伟赵翠萍综述李德富审校（中国药品生物制品检定所,北京１０００５０）分类号Ｒ５１１前言人类免疫缺陷病毒（Ｈｕｍａｎｉｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙｖｉｒｕｓ,ＨＩＶ）可以在人类引起艾滋病（ＡＩＤＳ）。经过十几年的...     

人类免疫缺陷病毒Ⅱ型跨膜蛋白在大肠杆菌中的表达

人类免疫缺陷病毒Ⅱ型（ＨＩＶ－２）的跨膜蛋白ｇｐ３６,是从外膜蛋白前体中裂解的,它在诱导病毒与细胞膜的融合和合胞体的形成中起着重要的作用〔１〕。感染ＨＩＶ－１或ＨＩＶ－２的患者,可产生针对病毒结构蛋白（包括包膜表面糖蛋白和跨膜蛋白）的体液免疫〔２－４...     

香豆素类化合物--抗HIV天然药物

艾滋病（ＡＩＤＳ）是由人类免疫缺陷病毒（ＨＩＶ）引起的一种传染性疾病。目前主要用于临床治疗的药物,均属核苷酸类药物,以ＨＩＶ病毒的逆转录酶作为靶点。这些药物虽然对ＨＩＶ病毒反转录有明显的抑制作用,但选择性较差,对人体细胞的毒性大,有严重的副作用［７］...     

人免疫缺陷病毒Ⅰ型膜糖蛋白gp160人单链抗体

人获得性免疫缺陷综合症（ＡｃｑｕｉｒｅｄＩｍｍｕｎｏｄｅｆｉｃｉｅｎｃｙＳｙｎｄｒｏｍｅ,简称ＡＩＤＳ）主要是由人免疫缺陷病毒Ⅰ型（ＨＩＶ１）引起的严重危害人类健康的传染病。ＨＩＶ研究的最新进展揭示了膜糖蛋白ｇｐ１２０与其受体和中和抗体复合物晶体结...     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).