重组蛋白药物的生产技术进展

重组蛋白药物是生物药物中的核心产品,主要是通过基因工程菌来生产功能蛋白或其突变体,用于弥补体内蛋白的缺失,从而对疾病的治疗发挥关键作用。近年来,重组蛋白药物在疾病治疗中发挥作用越来越大,相关技术也发展迅速。通过综述重组蛋白药物的中上游生产流程,并重点分析了重组蛋白药物在表达系统、细胞培养、纯化和质量控制等环节的最新技术进展,展示了重组蛋白药物制备的技术提升水平,以期为国内重组蛋白药物的生产提供一定的参考依据。     

我国基因工程药物产业化进程中存在的问题

我国基因工程药物产业化进程中存在的问题丁锡申（中国药品生物制品检定所）我国基因工程药物的研究和开发起步较晚,从“六五”开始至“八五”末期有了较快的发展,尤其在国家“八六三”高技术发展计划的优先支持下,确定了以产品为目标的策略,增力。了资金投入,优选了重卢、目标产品,使基因工程药品的研究和开发进入了一个蓬勃发展的新时期,产业化的进程,形势十分喜人。     

“蛋白质工程”一节教学设计

在"蛋白质工程"一节教学中,以《普通高中生物学课程标准(2017年版)》为依据,采用"科学-技术-社会"的教学策略,由胰岛素的工业合成引入话题,通过资料分析和小组讨论等方式,引导学生形成"蛋白质工程是基因工程的延伸"的核心概念,提高基于已有知识和新信息解决实际问题的能力,关注基因工程在药物生产中的实际应用,认同基因工程和蛋白质工程等生物技术的应用价值和发展前景。     

基因工程综合实训教学改革与创新

为培养学生的岗位职业能力,实现与岗位零对接,基因工程综合实训以工作任务为线索确定教学内容,以产品生产流程为载体设计教学过程,以职业能力为依据改革教学方法,以发展性评价为手段,强化技能形成。实践证明,"基因工程综合实训"教学改革,有利于培养学生的动手能力和实践创新能力,有利于促进以职业能力培养为核心的教育。     

国内外基因工程制药综述

自 1 972年ＤＮＡ重组技术诞生以来 ,生命科学进入了一个崭新的发展时期。以基因工程为核心的现代生物技术已应用到农业、医药、轻工、化工、环境等各个领域。它与微电子技术、新材料和新能源技术一起 ,并列为影响未来国计民生的四大科学技术支柱 ,而利用基因工程技术开发新型药物更是当前最活跃和发展迅猛的领域[1] 。从 1 982年美国Ｌｉｌｌｙ公司首先将重组人胰岛素 (商品名Ｈｕ ｍｕｌｉｎ)投放市场 ,标志着世界第一个基因工程药物的诞生以来 ,基因工程制药作为一个新兴行业得到各国政府的大力支持 ,各国都积极研究和开发各种基因工程药…     

活性污泥性质对基因工程菌吸附影响研究

基因工程菌主要是利用DNA重组技术所得,在DNA重组技术导向下可以达到选育优良菌株的目的并可按照意向对基因进行点缀从而得到特定的药物类菌株即为基因工程菌。基因工程菌可得到特异化产品以并让产品的生产水平得到大幅度提升,正是基于上述特点使其近年来受到愈来愈广泛的关注。在废水强化处理过程中对于基因工程菌而言活性污泥性质对其生存具有密切的关联性。相关研究证实基因工程菌吸附于污泥絮体后可让基因工程菌的生存能力得到大幅度提升。基于上述观点本文分析了活性污泥性质对基因工程菌吸附影响,供以参考。     

化学工业出版社推荐新书

本书是《现代微生物技术丛书》中的一个分册。全书系统论述了药物微生物技术的概念、研究内容和具体的应用技术。在介绍和归纳药物微生物、微生物药物和药物微生物技术的概念和内容的基础上,详细论述了药源微生物药物的筛选技术以及药物发酵合成的生产技术,概述了微生物技术在基因工程药物和疫苗中的应用,对次级代谢产物合成的基因工程改造(次级代谢工程)和药物微生物基因技术等新方法进行了分析和讨论。     

基因工程制药的研究现状与发展前景

简要叙述了近年来基因工程技术在医药工业中的应用进展,主要包括基因工程技术在药物生产、新药开发及药物生产工艺改进的应用.随着基因组和蛋白质组研究的深入,基因工程药物将有更多的机会获得突破性进展.     

本期导读

医药生物技术是生物技术首先取得突破、实现产业。化的技术领域,各种生物技“术产品被广泛应用于医疗、制药等行业,医药生物技术。已成为高技术产业发展的核心动力之一。近年来,我国医药生物技术研发和产业化取得了长足的进展。在基因工程药物和疫苗、单克隆抗体药物的研制。     

《药物微生物技术》

本书是《现代微生物技术丛书》中的一个分册．全书系统论述了药物微生物技术的概念、研究内容和具体的应用技术．在介绍和归纳药物微生物、微生物药物和药物微生物技术的概念和内容的基础上,详细论述了药源微生物和微生物药物的筛选技术以及药物发酵合成的优化和生产技术,概述了微生物技术在基因工程药物和疫苗中的应用,对次级代谢产物合成的基因工程改造(次级代谢工程)和药物微生物基因组技术等新方法进行了分析和讨论．本书在顾及药物微生物技术传统内容的同时,将更多的篇幅用于介绍和归纳各种新的技术方法和思维策略,     

Peer teaching and project planning

While project work is now widespread in higher education due consideration has not been given to the development of the intellectual skills on which such work depends. As an aid to better project work peer teaching has advantages in both cost and effectiveness. However, to be effective, students must be able to call on a procedure for analysing and representing clearly the structure of the situation with which they are concerned. This can be achieved by using a simplified version of Forrester dynamic systems models. Examples are described of some typical situations in which this approach has been successfully applied.     

The journey from NADPH-cytochrome P450 oxidoreductase to nitric oxide synthases

This mini-review will reflect the perspective of its author on two fields of research, which have merged as the result of the insights of investigators whose work has influenced both areas immeasurably. It cannot be overlooked, however, that the research activities of many during a period of over five decades have produced the chemical and biological bases for the exciting discoveries now encompassing the cytochromes P450 and their redox partners, and the three isoforms of nitric oxide synthase as they function in their respective biological milieux. Following the remarkable discovery that, indeed, molecular oxygen can be adducted to organic molecules by enzymatic systems and that such processes require a supply of reducing equivalents, it is the purpose of this review to provide a chart, with some of its detours, of the road that followed in the pursuit of interesting biological phenomena involving these two major oxygenation systems. It is not intended to be a balanced review and apologies must be offered in advance to those whose contributions may be overlooked or simply were not directly germane to the development of the author's journey.     

Biosensor technology: technology push versus market pull

Biosensor technology is based on a specific biological recognition element in combination with a transducer for signal processing. Since its inception, biosensors have been expected to play a significant analytical role in medicine, agriculture, food safety, homeland security, environmental and industrial monitoring. However, the commercialization of biosensor technology has significantly lagged behind the research output as reflected by a plethora of publications and patenting activities. The rationale behind the slow and limited technology transfer could be attributed to cost considerations and some key technical barriers. Analytical chemistry has changed considerably, driven by automation, miniaturization, and system integration with high throughput for multiple tasks. Such requirements pose a great challenge in biosensor technology which is often designed to detect one single or a few target analytes. Successful biosensors must be versatile to support interchangeable biorecognition elements, and in addition miniaturization must be feasible to allow automation for parallel sensing with ease of operation at a competitive cost. A significant upfront investment in research and development is a prerequisite in the commercialization of biosensors. The progress in such endeavors is incremental with limited success, thus, the market entry for a new venture is very difficult unless a niche product can be developed with a considerable market volume.     

Research ethics: the case with the tule (kuna), Urabbá, Colombia

An ethical analysis was performed during the development of a research project titled "Culture, nourishment and malaria in the Kuna aboriginals of Urabá, Colombia." This project had 2 objectives; (1) to investigate the prevalence of malaria and its relationship with hypovitaminosis A and nutritional status, and (2) to understand the community's conceptions and practices towards malaria. The current paper summarizes reflections concerning the application of ethical principles and moral values during the course of the malaria research project. Two intercultural relationships were clearly defined: the culture of the Tule (Kuna) Indians, with a traditional medical system, and the culture of the research group, focused on the biomedical model. The rationale and the discussion proposed during the writing of the project proposal was presented as well as the dilemmas that developed during the project within the communities. These problems required rapid decision-making in situations for which the scientists had not been adequately prepared. A need was indicated for application of ethical principles to permeate the analysis throughout the research process in a horizontal manner, such that the human dignity of the participants and the ethnicity of the community are preserved above the purposes of the project. In the research process, mediation between the ethics of conviction and the ethics of consequences must take precedence, with both outcomes tied to an informed consent in place during the process. The establishment of national and local research ethics committees was indicated as necessary to preserve the lifestyle and culture of the ethnic groups. These values were seen as endangered given the trading pressures and health policies in a globalized world.     

The caloric returns to food collecting: Disruption and change among the Batak of the Philippine tropical forest

The changing economy of a group of tropical forest hunter-gatherers is examined. The Batak of the Philippines today earn many calories by participating in an external market system; once they would have earned these calories within the traditional subsistence system. Gathering and selling Manila copal to purchase rice has partially displaced digging wild yams as the major source of subsistence. Data show that this change has reduced the productive efficiency, of human labor. The Batak must work longer and perform more difficult tasks to obtain the same number of calories by collecting copal as they can obtain by digging wild yams, even as they must today work longer hours anyway to earn necessary cash to satisfy new, nonfood consumer wants. The growing dietary importance of rice is only one aspect of changing food consumption patterns that may be having an unfavorable impact on Batak nutrition.An Arizona State University Faculty Research Grant supported research among the Batak between 1 July and 6 August 1975. A short summary of the research results discussed here appears inBorneo Research Bulletin, April 1976.     

A "how to" guide for developing a publishable Scholarship of Teaching project

In order for the efforts of scholarly teaching to be recognized, the work must become public and presented to peers for review. Scholarship of Teaching is not only improving instruction and learning but also methodically assessing whether specific teaching interventions have had the desired effect. In this paper, the author presents a step-by-step guide for how to develop a Scholarship of Teaching project that is well thought out and worthy of publication. Factors to consider before performing such pedagogical research include developing a clear research hypothesis for the classroom, reviewing the background literature, obtaining Institutional Review Board (Human Subjects) approval, and determining which methods of assessment may be used. This "how to" guide discusses how to handle all of these factors and prepare the data for publication and introduces the reader to references related to the Scholarship of Teaching and learning as well as educational research and theory.     

地市级大型公立医院临床研究型发展模式可行性研究

地市级大型公立医院选择临床研究型发展模式是国家政策的引导、区域医疗的需求以及医疗市场竞争和医疗机构自身发展的需要。确立“临床研究型医院”战略、建设“临床研究型医院”管理体系、学科人才、创新平台和文化氛围是“临床研究型医院”的主要发展路径。同时,建设“临床研究型医院”必须妥善处理好临床医疗与科研教学、规模扩张与内涵提升、自主创新与引进吸收的关系。     

Knowledge sharing and collaboration in translational research, and the DC-THERA Directory

Biomedical research relies increasingly on large collections of data sets and knowledge whose generation, representation and analysis often require large collaborative and interdisciplinary efforts. This dimension of 'big data' research calls for the development of computational tools to manage such a vast amount of data, as well as tools that can improve communication and access to information from collaborating researchers and from the wider community. Whenever research projects have a defined temporal scope, an additional issue of data management arises, namely how the knowledge generated within the project can be made available beyond its boundaries and life-time. DC-THERA is a European 'Network of Excellence' (NoE) that spawned a very large collaborative and interdisciplinary research community, focusing on the development of novel immunotherapies derived from fundamental research in dendritic cell immunobiology. In this article we introduce the DC-THERA Directory, which is an information system designed to support knowledge management for this research community and beyond. We present how the use of metadata and Semantic Web technologies can effectively help to organize the knowledge generated by modern collaborative research, how these technologies can enable effective data management solutions during and beyond the project lifecycle, and how resources such as the DC-THERA Directory fit into the larger context of e-science.     

Assuring the quality, safety, and efficacy of DNA vaccines

Scientists in academia whose research is aimed at the development of a novel vaccine or approach to vaccination may not always be fully aware of the regulatory process by which a candidate vaccine becomes a licensed product. It is useful for such scientists to be aware of these processes as the development of a novel vaccine could be problematic owing to the starting material often being developed in a research laboratory under ill-defined conditions. This paper examines the regulatory process with respect to the development of a DNA vaccine. DNA vaccines present unusual safety considerations that must be addressed during preclinical safety studies, including adverse immunopathology, genotoxicity through integration into a vaccinees chromosomes, and the potential for the formation of anti-DNA antibodies.     

Frontiers in Outreach and Education: The Florida Red Tide Experience

To enhance information sharing and garner increased support from the public for scientific research, funding agencies now typically require that research groups receiving support convey their work to stakeholders. The National Institute of Environmental Health Sciences-(NIEHS) funded Aerosolized Florida Red Tide P01 research group (Florida Red Tide Research Group) has employed a variety of outreach strategies to meet this requirement. Messages developed from this project began a decade ago and have evolved from basic print material (fliers and posters) to an interactive website, to the use of video and social networking technologies, such as Facebook and Twitter. The group was able to track dissemination of these information products; however, evaluation of their effectiveness presented much larger challenges. The primary lesson learned by the Florida Red Tide Research Group is that the best ways to reach specific stakeholders is to develop unique products or services to address specific stakeholders needs, such as the Beach Conditions Reporting System. Based on the experience of the Group, the most productive messaging products result when scientific community engages potential stakeholders and outreach experts during the very initial phases of a project.