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彩绒革盖菌漆酶产酶条件研究 总被引:8,自引:0,他引:8
本文研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响;结果表明,淀粉作碳源、干酷素作氮源有利于漆酶的分泌,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用;pH在3.0-8.0的范围内对漆酶的分泌影响差别不大,培养温度,接种量、通气量对漆酶的分泌有较大影响。 相似文献
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培养条件对毛栓菌漆酶分泌的影响 总被引:2,自引:0,他引:2
研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响;结果表明,麦草粉作碳源、(NH_4)_2SO_4作氮源有利于漆酶的分泌,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用;pH在3.0~8.0的范围内对漆酶的分泌影响差别不大,培养温度、接种量、通气量对漆酶的分泌有较大影响。 相似文献
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本文研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响;结果表明,淀粉作碳源、干酪素作氮源有利于漆酶的分泌,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用;pH在3.0~8.0的范围内对漆酶的分泌影响差别不大,培养温度、接种量、通气量对漆酶的分泌有较大影响。 相似文献
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研究了碳源、氮源、愈创木酚、香兰素及培养条件对漆酶分泌的影响 ;结果表明 ,麦草粉作碳源、(NH4 ) 2 SO4 作氮源有利于漆酶的分泌 ,适宜浓度的愈创木酚和香兰素等对漆酶的产生有一定的作用 ;pH在 3 0 - 8 0的范围内对漆酶的分泌影响差别不大 ,培养温度、接种量、通气量对漆酶的分泌有较大影响。漆酶最适pH值为 4 0 ,最适反应温度为 30℃ ,K+ 、Zn2 + 、Cu2 + 离子可激活漆酶 ;而Ag+ 、Fe3+ 、Cl- 离子可抑制漆酶活性。漆酶的Km值为 1 81× 10 - 3mol L。 相似文献
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高酶活菌株的筛选及漆酶特性 总被引:5,自引:0,他引:5
通过Bavendamn氏反应和液体发酵实验筛选出漆酶高产菌株 ,并对其产酶条件和酶活性进行了研究。结果表明 71株实验真菌中有 64株Bavendamn氏反应呈阳性 ,且阳性菌株都具有漆酶活性 ;不同菌株产酶培养基最适碳源、氮源不同 ,采绒革盖菌以淀粉为碳源、干酪素为氮源 ,毛栓菌以麦草粉为碳源、硫酸铵为氮源 ,有利于酶的分泌 ;不同来源漆酶性质不尽相同 ,采绒革盖菌漆酶最适酶解温度为 2 5℃ ,最适酶解pH值为4.6,毛栓菌则分别为 3 0℃和 pH 4.0 ;K+ ,Zn2 + 等对 2种漆酶均有激活作用 ,Ag+ 则能明显抑制漆酶活性。 相似文献
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采用单因子相互比较法研究了不同碳素和氮素对彩绒革盖菌胞外漆酶,愈创木酚氧化酶,多酚氧化酶,锰过氧化物酶等木素降解酶分泌的影响,结果淀粉作碳源,干酪素作氮源有利于漆酶的分泌,麦芽粉作碳源,酵母膏作氮源有利于愈创木酚氧化酶和多酚氧化酶的分泌,淀粉作碳源,玉米粉作氮源有利于锰过氧化物酶的分泌。 相似文献
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蜜环菌胞外漆酶的合成、纯化及性质研究 总被引:9,自引:0,他引:9
研究了蜜环菌胞外漆酶合成条件和酶学性质。实验表明,培养基初始pH5.5、培养温度25℃有利于菌株产酶;与麦芽糖、山梨糖和半乳糖相比,纤维二糖和棉子糖作为碳源时漆酶产量更高;有机氮源比无机氮源有利于漆酶合成。泥炭提取液可显著诱导漆酶生成,当其含量为50%时,菌株漆酶最高产量是对照组的7倍。在蜜环菌发酵上清液中检测到3个漆酶同功酶组分,其主要活性(约占75%)组份漆酶A经 (NH4)2SO4沉淀、制备级PAGE电泳和阴离子交换柱层析被分离纯化至电泳均一,SDSPAGE法测得酶亚基分子量59kD,凝胶过滤色谱法测定活性酶分子量58kD。纯化的漆酶A等电点pI为4.0,氧化愈创木酚的最适反应pH为5.6,最适温度为60℃,在60℃和65℃时半衰期分别为45min和36.8min,在pH5.2~7.2范围内稳定性较好。100mmol/L Cl-对该酶有显著抑制作用,1mmol/L SO2-4 对漆酶有激活作用,1mmol/L NaN3可完全抑制酶活性,10 mmol/L EDTA对漆酶活没有明显影响,1mmol/L Cu2+对漆酶有激活作用。以愈创木酚为底物时,测得酶的Km=1.026mmol/L,Vmax=5μmol/(min·mg);以ABTS为底物时,测得其Km=0.22mmol/L,Vmax=69μmol/(min·mg)。 相似文献
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从实验室保存的7株真菌筛选到1株能高效降解甲苯的菌株H1,基于形态特征、ITS序列系统学分析,将H1菌株鉴定为毛栓菌(Trametes hirsuta)。利用正交设计实验方法研究了温度、pH值、甲苯浓度和吐温80浓度对H1菌株降解甲苯的影响,研究得出该菌株降解甲苯的最适条件为30℃、pH 5.0、甲苯浓度300mg/L、吐温80浓度0.05%,在该条件下H1对甲苯的最大降解率为85.3%,降解率比未优化之前有了显著提高。比较了H1菌株在3种培养基产生漆酶的能力,H1在土豆葡萄糖培养基产酶能力最强,在第7天达到酶活高峰16 500 U/L。H1在甲苯为唯一碳源的培养基中,漆酶酶活最低,培养7 d时漆酶酶活为589 U/L。 相似文献
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以白腐真菌模式菌株黄孢原毛平革菌Phanerochaete chrysosporium为研究对象,探讨培养条件、重金属和芳香族化合物对产漆酶的影响,并进一步研究漆酶对刚果红的降解效果。结果表明,P. chrysosporium产漆酶最适培养条件:葡萄糖为碳源,蛋白胨为氮源,碳氮比为90。培养8d后,漆酶酶活为911.1U/L;Mn2+严格地控制着P.chrysosporium产漆酶,而Cu2+对其影响不大,在添加4.0mmol/L Mn2+时,漆酶酶活为2 001.7U/L;芳香族化合物中藜芦醇(veratryl alcohol,VA)、4-香豆酸、香草醛和香草酸对菌体产漆酶能力均有促进作用,最高可提升至1 459.1U/L,而咖啡酸对菌体产漆酶稍有抑制。100U/L漆酶粗酶液可降解40mg/L刚果红,降解率为24.0%;而当介体物质VA存在时,该降解效率可提升至87.7%。 相似文献
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The effects of the carbon and nitrogen sources, initial pH and incubation temperature on laccase production by the endophytic fungus Monotospora sp. were evaluated. The optimal temperature and initial pH for laccase production by Monotospora sp. in submerged culture were found to be 30 degrees C and 8.5, respectively. Maltose (2 g l(-1)) and ammonium tartrate (10 g l(-1)) were the most suitable carbon and nitrogen source for laccase production. Under optimal culture medium, the maximum laccase activity was determined to be 13.55 U ml(-1), which was approximately four times higher than that in basal medium. This is the first report on laccase production by an endophytic fungus. 相似文献
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Summary The white rot fungus Coriolus versicolor MTCC 138 has been identified as an excellent producer of the industrially important enzyme laccase. The basal medium containing glucose gave laccase activity of 155 U/ml. Screening of different media components and their effects on laccase production by Coriolus versicolor was studied using one factor at a time and L9 (34) orthogonal array method. A two-fold increase (305 U/ml) in laccase production was observed using a combination of glucose and starch as carbon source and yeast extract as nitrogen source. This activity is very high as compared to most of the reported strains. Hence this strain was explored for enhancement in laccase. The formation of extracellular laccase could be considerably stimulated by the addition of copper in the optimized medium. Addition of 1 mM copper enhanced laccase activity to 460 U/ml. Laccase production was further enhanced using different aromatic inducers. Among different inducers used 2,5-xylidine was found to be the best, and gave maximum laccase activity of 820 U/ml with 1 mM concentration. Thus, this strain could be an efficient and attractive source for laccase production. 相似文献
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Churapa Teerapatsakul Roberto Parra Christopher Bucke Lerluck Chitradon 《World journal of microbiology & biotechnology》2007,23(11):1519-1527
To engineer the production of laccase by Ganoderma sp. KU-Alk4, a newly isolated white-rot fungus, a seven-level Box−Behnken factorial design was employed to optimize the culture
medium composition. A mathematical model was developed to show the effect of each medium component and their interactions
on the production of laccase activity in submerged fermentation. The model estimated the optimal concentrations of glycerol,
yeast extract and veratryl alcohol as 40, 0.22 g/l and 0.85 mM, respectively, with the medium pH of 6.0. These predicted conditions
were verified by validation experiments. The optimized medium gave laccase activity of 240 U/ml, which is 12 times higher
than that produced in non-optimized medium. Thus, this statistical approach enabled rapid identification and integration of
key medium parameters for Ganoderma sp. KU-Alk4, resulted the high laccase production. 相似文献
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粗毛栓菌(Trametes hirsuta) D2固态发酵山核桃蒲壳产漆酶的营养条件研究 总被引:1,自引:0,他引:1
【目的】为提高漆酶产量,降低生产成本,以山核桃蒲壳作为基质,对粗毛栓菌D2固态发酵产漆酶的营养条件进行研究。【方法】对不同碳源、氮源、碳氮比、蒲壳含量对漆酶产量的影响进行分析。【结果】山核桃蒲壳是粗毛栓菌生长的良好载体,能够促进漆酶的合成。粗毛栓菌D2漆酶固态发酵培养基干物质组成为:山核桃蒲壳40%(质量比),玉米粉24%(质量比),菜籽饼粉36%(质量比)。发酵6 d时,漆酶活性为126.8 U/g干基。【结论】粗毛栓菌固态发酵山核桃蒲壳产漆酶具有效率高,生产成本低的优点,具有潜在的工业化应用前景。 相似文献
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Fomes sclerodermeus produces manganese peroxidase (MnP) and laccase as part of its ligninolytic system. A Doehlert experimental design was applied in order to find the optimum conditions for MnP and laccase production. The factors studied were Cu2+, Mn2+ and asparagine. The present model and data analysis allowed us not only to define optimal media for production of both laccase and MnP, but also to show the combined effects between the factors. MnP was strongly influenced by Mn2+, which acts as an inducer. Under these conditions Cu2+ negatively affected MnP activity. At 13 days of growth 0.75 U ml–1 were produced in the optimized culture medium supplemented with 1 mM MnSO4 and 4 g l–1 asparagine. The laccase titer under optimized conditions reached maximum values at 16 days of growth: 13.5 U ml–1 in the presence of 0.2 mM CuSO4, 0.4 mM MnSO4 and 6 g l–1 asparagine. Mn2+ promoted production of both enzymes. There were important interactions among the nutrients evaluated, the most significant being those between Cu2+ and asparagine. 相似文献