卵巢癌中线粒体转录终止因子1的表达及其意义

目的:探讨人线粒体转录终止因子1(MTERF1)在卵巢癌中的表达及其在预后判断中的作用。方法:利用Oncomine数据库对比分析卵巢癌和正常卵巢组织中MTERF1基因表达水平;提取10例配对卵巢癌组织和正常卵巢上皮组织总RNA和蛋白质,real time RT-PCR检测MTERF1 mRNA在各组织中的表达,Western印迹检测MTERF1蛋白在各组织中的表达;利用Kaplan-Meier plotter数据库分析MTERF1表达对卵巢癌患者的预后价值。结果:Oncomine数据库中共收集了8个不同类型的研究结果,荟萃分析显示MTERF1在卵巢癌中表达增高,且差异具有统计学意义(P0.05)。本研究10例卵巢癌及其配对的正常卵巢上皮组织中,有6例卵巢癌中MTERF1 mRNA和蛋白质表达水平增高。Kaplan-Meier plotter数据库分析显示,MTERF1 mRNA表达水平越高,早于2期的卵巢癌患者无进展生存期越短,预后越差(P0.05);但卵巢癌患者的总生存期与MTERF1 mRNA表达水平无相关性(P0.05)。结论:卵巢癌组织中MTERF1 mRNA和蛋白质表达升高;在早期卵巢癌患者中,MTERF1表达水平越高,预后越差,可作为预测卵巢癌患者预后的潜在分子标记物。     

基于Oncomine和TCGA数据挖掘分析MTERF2在胰腺癌中的表达及临床意义

目的:探究人线粒体转录终止因子2(MTERF2)在胰腺癌中的表达及临床意义。方法:检索Oncomine数据库中的MTERF2信息,对其在胰腺癌组织和正常胰腺组织中的表达进行荟萃分析;从美国癌症基因组图谱(TCGA)中下载胰腺癌数据集MTERF2 RNA Seq V2数据及临床病理资料;利用R 2.15.3软件分析MTERF2表达量与临床病理参数的相关性及预后;利用Kaplan-Meier生存函数分析MTERF2表达量和胰腺癌患者预后的关联。结果:Oncomine数据库中共收集了586个不同类型的研究结果,其中关于MTERF2表达有统计学差异的研究有58个,MTERF2表达增高的研究有26个,表达减低的研究有32个。共有9项研究涉及MTERF2在胰腺癌组织和正常组织中的表达,包括226例临床样本,与对照组相比,MTERF2在胰腺癌中低表达(P=7.34×10-6)。TCGA胰腺癌数据集中共收集179例具有临床病理参数的病例及其对应的MTERF2 mRNA表达量。剔除病理参数不详或不完整的病例,利用R 2.15.3软件分析发现,MTERF2 mRNA表达量与胰腺癌患者T分期、M分期、癌症类型及原发部位存在显著相关(均P<0.05),而与患者的年龄、性别、N分期、AJCC病理分期、等级、组织学类型及饮酒史无显著相关(均P>0.05)。Kaplan-Meier生存分析发现,胰腺癌患者MTERF2的表达水平与总生存率(OS)无显著相关性(P>0.05),而与无病生存率(DFS)显著相关(P<0.001)。结论:Oncomine和TCGA数据挖掘显示,人MTERF2在胰腺癌组织中低表达,且MTERF2表达量与胰腺癌患者总生存率无显著相关性,与无病生存率有显著相关性。     

基于ONCOMINE数据库荟萃分析BRCA1基因在肺癌中的表达及意义

为探讨BRCA1基因在肺癌中的表达及对肺癌患者预后生存意义,本研究利用(BioGPS, Oncomine,Kaplan-Meier Plotter)基因数据库和癌症细胞系百科全书(CCLE),分析BRCA1基因在正常人体组织中的表达以及差异表达情况,并对符合条件的研究进行Meta分析,同时分析BRCA1基因在肺癌组织细胞系中的表达和对肺癌患者预后生存意义。研究发现,BioGPS数据库显示BRCA1在正常肺癌组织中低表达(3.65±0.035);同时Oncomine数据库检索BRCA1基因有453项不同种类结果,35项结果表达有统计学差异意义,其中28项显示表达增高,7项显示表达降低,共有研究样本量229例;并对符合条件的5项研究进行Meta分析,发现BRCA1基因差异表达中位数值排名为34.0,p=2.51×10~(-5);与CCLE检索BRCA1基因在肺癌组织细胞系中高表达的结果一致;Kaplan-Meier Plotter数据库检索结果表明BRCA1表达水平与患者的总生存预后周期相关(p=9.5×10~(-6));并且BRCA1高表达组相对于低表达组的肺癌患者总体生存周期明显降低(p0.05)。因此,通过深入挖掘Oncomine等数据库中BRCA1基因芯片信息,证实BRCA1基因在肺癌组织中呈现高表达状态,且与肺癌患者预后生存周期相关,为临床肺癌的治疗及基因靶向药物的研制提供重要依据。     

基于Oncomine数据库探究COL1A1基因在胰腺癌中的表达及意义

[目的]探究COL1A1基因在胰腺癌中的表达及临床意义。[方法]收集Oncomine和NCBI/GEO Profiles数据库中关于COL1A1的信息,对目前数据库中资料再次进行分析,并对其在胰腺癌组织和正常胰腺组织中的表达进行荟萃分析,使用Kaplan-Meier Plotter在线数据库分析该基因是否与胰腺癌患者的预后相关。[结果]Oncomine数据库中共收集了400项不同类型的研究。其中COL1A1高表达有86项研究,低表达有6项研究。共有8项研究涉及COL1A1在胰腺癌组织与正常组织中的表达,包括209例样本,与正常组织相比,COL1A1在胰腺癌组织中高表达(P<0.05)。NCBI/GEO Profiles数据库分析发现TGF-β处理48 h后COL1A1表达升高。Kaplan-Meier Plotter数据库分析表明COL1A1高、低表达组胰腺癌患者的总体生存率(OS)无统计学差异(HR=1.4,95%CI:0.92~2.12,P=0.11),但高表达组胰腺癌患者的无复发生存(RFS)明显差于低表达组(HR=5.05,95%CI:1.48~17.24,P=0.0044)。[结论]COL1A1基因在胰腺癌组织中的表达明显高于正常组织,低表达患者的RFS延长。可见靶向COL1A1的药物有望改善胰腺癌患者的预后。     

基于Oncomine数据库分析COL5A2基因在胰腺癌中的表达及其临床意义

目的:探讨COL5A2基因在胰腺癌中的表达及临床意义。方法:收集Oncomine和NCBI/GEO Profiles数据库中关于COL5A2的信息,对目前数据库中的资料进行二次分析,并对其在胰腺癌组织和正常胰腺组织中的表达进行荟萃分析,利用Kaplan-Meier Plotter数据库分析COL5A2与胰腺癌患者预后的关系。结果:Oncomine数据库中共收集了417项不同类型的研究,其中COL5A2高表达有75项研究,低表达有3项研究。共有7项研究涉及COL5A2在胰腺癌组织与正常组织中的表达,包括178例样本,与正常组织相比,COL5A2在胰腺癌组织中高表达(P0.05)。NCBI/GEO Profiles数据库分析同样发现COL5A2在胰腺癌组织中高表达(P0.05),与达沙替尼(dasatinib)敏感细胞株相比,达沙替尼耐药细胞株中COL5A2表达升高,进一步分析发现TGF-β处理48 h后COL5A2表达升高。Kaplan-Meier Plotter数据库分析表明COL5A2高、低表达组胰腺癌患者的总体生存率(OS)无统计学差异(HR=1.45,95%CI:0.96～2.18,P=0.077),但高表达组胰腺癌患者的无复发生存率(RFS)明显差于低表达组(HR=4.13,95%CI:1.46～11.72,P=0.0045)。结论:基于Oncomine和NCBI/GEO Profiles数据库分析发现COL5A2在胰腺癌组织高表达,且与胰腺癌患者的无复发生存及达沙替尼耐药性密切相关,有望成为胰腺癌诊断和治疗的重要靶标。     

基于数据挖掘分析ONECUT2基因在胃癌的表达及与幽门螺杆菌感染的相关性

目的分析ONECUT2基因在胃癌中的表达及其与幽门螺杆菌(Helicobacter pylori,H.pylori)感染的相关性。方法 (1)利用Oncomine数据库、TCGA数据库分析ONECUT2基因在胃癌中的表达,R软件包进行基因的共聚类微阵列数据分析。(2)利用Kaplan-Meier Plotter数据库和GEPIA数据库分析ONECUT2差异表达与胃癌患者预后的关系。(3)利用生物信息学功能注释数据平台DAVID对胃癌中与ONECUT2基因表达正相关的前200个基因进行功能富集分析。(4)利用GEO数据库中的数据分析H.pylori感染与ONECUT2表达的相关性。结果 (1)Oncomine数据库中的数据在胃癌、膀胱癌等8种肿瘤中共有14项研究显示ONECUT2基因在肿瘤组织中的高表达;其中胃癌相关的有两项(t=6.064,P0.000 1;t=4.335,P0.000 1);TCGA数据库的数据也证实胃癌中ONECUT2高表达(t=6.680,P0.001)。(2)胃癌中ONECUT2与EHF、FUT6、TNFRSF11A、RSAEF、EPCAM等20个基因有较高的共表达。(3)GO分析发现胃癌中ONECUT2富集在分子结构活性等相关的基因功能上;而KEGG富集分析发现ONECUT2富集于紧密连接功能等6个通路。(4)Kaplan-Meier Plotter数据库中207676-at芯片结果显示ONECUT2高表达组患者远期生存率下降(P0.001);GEPIA数据库中的分析结果同样显示ONECUT2高表达组患者其总体生存率(P0.05)和无病生存率(P0.01)下降。GEO数据库中GSE74577(P=0.005)、GSE70394(P=0.034)和GSE25146(P=0.036)三个数据集均显示H.pylori感染AGS和GES-1细胞系后ONECUT2表达上调。结论 ONECUT2在胃癌中高表达,且与胃癌一类致癌原H.pylori感染相关。ONECUT2高表达组患者远期生存率下降,表明其与胃癌发生发展密切相关,可能成为胃癌治疗的新靶点。     

基于数据挖掘分析MTERF2基因在子宫内膜癌的表达及意义

目的:基于数据挖掘分析线粒体转录终止因子2(MTERF2)基因在子宫内膜癌及正常子宫内膜组织中的表达情况,并进一步探讨MTERF2对子宫内膜癌患者预后的影响。方法:利用Oncomine数据库分析MTERF2基因DNA拷贝数在子宫内膜癌组织中的变化;利用cBioportal分析MTERF2基因在子宫内膜癌组织中的突变情况;通过基因表达谱动态分析(GEPIA)探讨人体正常组织及其相应的肿瘤组织中MTERF2基因的表达差异;经MethHC分析子宫内膜癌和正常子宫内膜组织中MTERF2基因DNA启动子区甲基化水平的差异;利用OncoLnc对MTERF2基因的表达水平与子宫内膜癌患者生存率做Kaplan-Meier生存分析和Log-Rank检验;在String-DB数据库中探索MTERF2基因在线粒体DNA表达调控过程中关系密切的相关基因。结果:与正常子宫内膜组织相比,子宫内膜癌组织中MTERF2基因DNA拷贝数无明显变化(P0.05),在mRNA水平呈显著低表达(P0.05),DNA启动子区甲基化水平显著降低(P0.005);MTERF2基因的表达水平与子宫内膜癌患者的总体生存时间无显著相关性(Log-Rank P0.05);TFB1M、TFB2M、POLMT、MTERFD1、MTERFD2、PTCD1、SSBP1、COA3、HDGFRP3等基因与MTERF2基因关系密切,可能存在相互作用。结论:大样本数据挖掘能迅速获取子宫内膜癌组织中MTERF2基因表达的相关信息,为深入探究MTERF2基因在子宫内膜癌发生发展中的作用机制及其预后价值奠定基础。     

幽门螺杆菌感染与胃癌相关基因的筛选及预后分析

目的通过分析幽门螺杆菌感染胃黏膜组织和胃癌细胞系后的差异基因变化,并在癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库和肿瘤基因芯片(Oncomine)数据库进行验证,探究幽门螺杆菌导致胃癌发生、发展的分子机制。方法分析基因表达汇编(Gene Expression Omnibus,GEO)数据库幽门螺杆菌感染相关芯片集GSE5081与GSE70394,绘制维恩图查找幽门螺杆菌感染后共同上调的差异基因。对共同上调的差异基因进行功能富集分析。通过TCGA和Oncomine数据库验证差异基因在胃癌中的表达。利用Kaplan-Meier Plotter数据库和GEPIA数据库分析差异基因表达高低与胃癌患者预后是否存在相关性。结果通过差异基因筛选和维恩分析,两个芯片集共有21个共同上调差异基因。GO分析发现共同上调差异基因主要富集在对细菌来源分子的反应、趋化因子CXCR受体结合、中性粒细胞趋化作用等相关的基因功能上;KEGG通路主要富集在癌症通路、TNF信号通路、趋化因子信号通路等。STRING以及PPI数据库分析发现21个基因中PRDM1、IL10、NRP1、BIRC3、GNG13、CXCL1、CXCL2、CXCL3、CXCL8基因存在有网络关系,属于关键枢纽基因。通过TCGA和Oncomine数据库筛选及验证,发现在胃癌组织中NRP1、CXCL1、CXCL8基因明显上调,结果差异有统计学意义(TCGA数据库中,三者P值均小于0.05,Oncomine数据库中,NRP1:t=4.607,P0.01;CXCL1:t=5.854,P0.01;CXCL8:t=5.316,P0.01)。在Kaplan-Meier Plotter数据库(210615-at芯片:P0.01;210510-s-at芯片:P0.01;212298-at芯片:P0.01)以及GEPIA数据库(P0.01)两个数据库中,NRP1的高表达均与胃癌的预后负相关。结论不同的数据库均显示NRP1、CXCL1、CXCL8三个基因与幽门螺杆菌感染相关,同时在胃癌中高表达,并且NRP1的高表达与胃癌的不良预后相关,这些结果为进一步探究幽门螺杆菌导致胃癌发生、发展的分子机制提供了重要基础。     

APRO家族基因在胃癌中的表达及预后意义

目的:基于大数据挖掘分析BTG/Tob抗增殖蛋白家族(anti-proliferativeprotein family,APRO)基因在胃癌组织的表达及其对胃癌患者预后的影响。方法:采用Oncomine数据库分析APRO家族6个成员在胃癌组织中的m RNA表达情况,通过Kaplan-Meier Plotter数据库进行胃癌患者总生存期的分析。结果:相比正常胃组织,BTG2在胃癌组织中呈低表达;BTG3在肠型胃癌组织中呈高表达,而在总体胃癌组织中呈低表达。BTG3低表达的患者总生存期较短;对5-氟尿嘧啶辅助化疗的胃癌患者,低表达BTG2的预后较差。结论:BTG2、BTG3的m RNA表达在胃癌和正常胃组织中有明显差异。BTG3低表达的胃癌患者预后较差;BTG2可能参与调节胃癌患者5-氟尿嘧啶治疗的敏感性。     

六种藓类植物茎的比较解剖学研究

通过对6种藓类植物,即褶叶青藓(Brachythecium salebrosum(Web.et Mohr.)B.S.G.)、湿地匐灯藓(Plagiomnium acutum(Lindb.)Kop.)、侧枝匐灯藓(Plagiomnium maximoviczii(Lindb.)Kop.)、大凤尾藓(Fissidensnobilis Griff.)、大羽藓(Thuidium cymbifolium(Doz.et Molk.)B.S.G.)和大灰藓(Hypnum plumaeforme Wils.)嫩茎和老茎的石蜡切片和显微观察发现,同一藓类植株的嫩茎和老茎,茎结构稳定,不同种藓类植物茎横切面具有不同特征.植物体茎横切面形状、表层细胞的层数、细胞大小和细胞壁厚薄、皮层细胞大小和形状、中轴的有无以及比例等特征可以作为藓类植物的分科分类依据之一.     

Photoregulation of seed germination of wild-type and of an aurea-mutant of tomato

Seed germination of an aurea mutant of tomato ( Lycopersicon esculentum Mill.) is promoted by continuous irradiation with red, far-red or long-wavelength far-red (758 nm) light as well as by cyclic irradiations (5 min red or 5 min far-red/25 min darkness). Far-red light applied immediately after each red does not change the germination behaviour. Seed germination of the isogenic wild-type, cv. UC-105, is promoted by continuous and cyclic red light while it is inhibited by continuous and cyclic far-red light and by continious 758 nm irradiation. Far-red irradiation reverses almost completely the promoting effect of red light. The promoting effect (in the aurea mutant) and the inhibitory effect (in the wild-type) of continuous far-red light do not show photon fluence rate dependency above 20 nmol m⁻² s⁻¹. It is concluded that phytochrome controls tomato seed germination throgh low energy responses in both the wild type and the au mutant. The promoting effect of continuous and cyclic far-red light in the au mutant can be attributed to a greater sensitivity to P_fr.     

Cause of Senescence of Nine Sorts of Flowers

The levels of endogenous phytohormones and respiratory rate in nine sorts of flowers such as Cymbidium faberi Rolfe, Nopalxochia ackermannii Kunth and others were investigated both at full bloom and senescence and meanwhile the effect of exogenous phytohormones on prolonging the blossoms and promoting ethylene production were tested. There is a high content of endogenous ethylene in all the long-lived flowere, about 3–16 folds higer than the short-lived ones. There is a high level of ABA at full blooming flowers of short-lived flowers, in which there is no or only some cytokinins in it, but the ratio of CTK (6BA+zeatin)/ABA is smaller(l.7). The endogenous ABA reached a much higher level at senescence in all nine sorts of flowers, so it is reasonable to consider that it is ABA which plays an important role of regulation in controlling flower's senescence. There is a much higher level of GA3 and zeatin in the long-lived flowers which is not demonstrated in the shortlived ones. The respiratory rate is one of the factors controtling the longevity of flowers, but it does not play a decided role. Application of 6BA and zeatin prolongs distinctly orchid’s longevity, however exogenous IAA through the promotive action on ethylene production, evidently extends the longevity of the flowers of the Nopalxochia ackermannii Kunth.     

Interconnection of parameters of regulation system of lipid peroxidation and of morphophysiological parameters of mouse liver

A complex analysis of seasonal fluctuations of the mean group parameters of the system of regulation of lipid peroxidation has been performed in liver of Balb/c mice. Association of lipid characteristics and morphophysiological parameters is studied in the Balb/c mouse liver. An inter-connection is revealed between the liver index and the amount of lysoforms of phospholipids, the scale and character of the interconnection differing essentially depending on proportion of phos-phatidylcholine in mouse liver phospholipids.     

龙胆科药用植物化学成分的研究现状

龙胆科植物在我国的分布范围很广,且多数为药用植物,其多数种属的药用植物,至今其化学成分尚未被系统研究。综述了目前龙胆科药用植物的化学成分的研究现状及一般提取方法,对近年来发现的环烯醚萜及裂环烯醚萜类化合物进行了总结,为本科药用植物的更深入研究提供了参考。     

Physiological characteristics of various species of strains of carboxydobacteria

Seven strains of aerobic carbon monoxide-oxidizing bacteria (carboxydebacteria) when growing on CO as sole source of carbon and energy had doubling times which ranged from 12–42 h. The activity profiles obtained after discontinuous sucrose density gradient centrifugation indicated that the CO-oxidizing enzymes are soluble and the hydrogenases are membrane-bound in all strains examined. The CO-oxidizing enzymes of Pseudomonas carboxydohydrogena, Pseudomonas carboxydoflava, Comamonas compransoris, and the so far unidentified strains OM2, OM3, and OM4 had a molecular weight of 230,000; that of Achromobacter carboxydus amounted to 170,000. The molecular weights of the CO-oxidizing and H₂-oxidizing enzymes turned out to be identical. The cell sonicates were shown to catalyze the oxidation of both CO and H₂ with methylene blue, thionine, phenazine methosulfate, toluylene blue, dichlorophenolindophenol, cytochrome c or ferricyanide as electron acceptors. Methyl viologen, benzyl viologen, FAD⁺, FMN⁺, and NAD(P)⁺ were not reduced. The spectrum of electron acceptors was identical for all strains tested. Neither free formate, hydrogen nor oxygen gas were involved in the CO-oxidation reaction. Methylene blue was reduced by CO at a 1:1 molar ratio. The results indicate that CO-oxidation by carboxydobacteria is catalyzed by identical or similar enzymes and that the reaction obeys the equation CO+H₂OCO₂+2H⁺+2e^- as previously shown for Pseudomonas carboxydovorans.Dedicated to Otto Kandler remembering almost three decades of enjoyable cooperation     

Modulation of allostery of pyruvate kinase by shifting of an ensemble of microstates

Since the introduction of the concepts of allostery about four decades ago, much advancement has been made in elucidating the structure-function correlation in allostery. However, there are still a number of issues that remain unresolved. In this review we used mammalian pyruvate kinase (PK) as a model system to understand the role of protein dynamics in modulating cooperativity. PK has a triosephosphate isomerase (TIM)(α/β)₈ barrel structural motif. PK is an ideal system to address basic questions regarding regulatory mechanisms about this common (α/β)₈ structural motif. The simplest model accounting for all of the solution thermodynamic and kinetic data on ligand-enzyme interactions involves two conformational states, inactive E^T and active E^R. These conformational states are represented by domain movements. Further studies provide the first evidence for a differential effect of ligand binding on the dynamics of the structural elements, not major secondary structural changes. These data are consistent with our model that allosteric regulation of PK is the consequence of perturbation of the distribution of an ensemble of states in which the inactive E^T and active E^R represent the two extreme end states. Sequence differences and ligands can modulate the distribution of states leading to alterations of functions. The future work includes: defining the network of functionally connected residues; elucidating the chemical principles governing the sequence differences which affect functions; and probing the nature of mutations on the stability of the secondary structural elements, which in turn modulate allostery.     

（鱼句）亚科花（鱼骨）型鱼类骨骼系统的比较

对我国花型Hemibarbuspattern鱼类作了骨骼系统比较,结果表明,此类型鱼类脑颅较长,副蝶骨平直或稍弯曲,眶蝶骨腹纵嵴发达(铜鱼Coreius septentrionalis例外),下颞窝和咽突中等大,基枕骨后突发达;脑颅中的上筛骨的后突、侧筛骨的外筛突,蝶耳骨的外突、上耳骨的后突、围眶骨和后颞窝等均有明显的差异;咽颅中的舌颌骨、尾舌骨、鳃盖骨和下咽齿的列数等又有显著的区别;附肢骨骼中的腰带骨、脊椎骨中的复合神经骨和第4椎骨腹侧的悬器等也有不同之处。据此,这些差异和区别可作为属间或种间的分类依据。