基于水声学方法的天目湖鱼类季节和昼夜空间分布研究

采用水声学方法系统地对天目湖春夏秋冬四季鱼类水平和垂直空间分布进行了调查研究,同时对昼夜探测的差异性进行了比较,并对不同季节间鱼类聚群形态进行了探讨。研究结果表明,天目湖鱼类在不同季节间存在规律性水平迁移,从全湖角度分析,天目湖鱼类资源昼夜间的水平空间分布无明显差异特征,但在春末和夏季的局部区域里,昼夜间鱼类存在一定近岸—远岸的水平迁移;不同季节和昼夜间,鱼类垂直分布差异明显,且在存在温跃层的夏季7月昼间,鱼类密度垂直分布与水温的垂直分布关系密切,温跃层以下的鱼类密度基本为0;天目湖鱼类在四个季节都属于成群分布类型,但季节间鱼类聚群形态不同,在冬季的1月呈现出典型的聚集,相对于昼间,春夏季鱼类在夜间分布更为均匀、分散,且水平探测表明在夜间水体表层存在大量鱼类分布。     

三峡库区大宁河鱼类的时空分布特征

为了解大宁河鱼类资源量及其分布特点,于2006年12月和2007年4月利用HTI Model 241鱼探仪对三峡库区大宁河进行了声学调查。结果表明：冬季鱼类密度显著高于春季,这与鱼类自身的繁殖越冬行为有关。统计分析显示,鱼类水平分布的差异与水深没有明显的相关性,可能主要由群聚行为引起。另外,冬季大宁河鱼类主要分布在中下层,上层和中下层的鱼类密度分布存在极显著性差异(p <0.01);春季鱼类主要分布在中上层,中层和下层的鱼类密度分布差异显著(p <0.05)。大宁河鱼类在冬季和春季均属于成群分布,并且春季各层鱼类密度的变异系数高于冬季,这与水温和鱼类的防御机制有关。     

我国伊犁河鱼类群落结构及外来鱼类适生性

2012-2019年,在我国伊犁河流域的6个断面开展了鱼类群落结构调查,结果显示我国伊犁河流域现有鱼类共计29种,大多属于鲤形目。鱼类群落组成与20世纪末期相比发生了变化,银色裂腹鱼、短头鲃、银鲫、鲢、北方泥鳅在本研究中未出现,尤其是银色裂腹鱼,截至目前为止的鱼类资源调查中,均无捕获记录。历史研究中未见的乌鳢与大口鲇在本研究中有捕获。外来鱼类的出现率和数量均有所增加。对鱼类群落结构特征进行分析,显示伊犁河流域鱼类群落多样性较低,下游干流和上游支流的鱼类群落结构差异较大。现阶段,我国伊犁河流域主要的优势种外来鱼类有鲤、和鲫。利用最大熵生态位模型（MaxEnt）对鲤、和鲫的适生区域进行预测,与鱼类资源调查获得的鱼类实际分布区对比,显示鲤和在我国伊犁河流域的分布范围与预测结果是吻合的,而鲫在进入伊犁河流域之后开始向上游支流河段扩散。由于这几种外来鱼类在一开始引入的时候未采取管控措施,又具有极强的适应能力,且伊犁河流域的鱼类多样性较小,因此极易形成规模并扩散。外来鱼类的入侵,会对土著鱼类造成巨大的威胁。基于本论文研究结论,应当将已发生生态位漂变的鲫其作为重点关注种类,关注外来鱼类的生活史对策,深入研究外来鱼类的入侵机制和诱因;对上游支流河段的生境进行监测,关注气候和水文变化,尤其是土著鱼类产卵场、索饵场和越冬场的变化,尽可能减小人类活动干扰;对伊犁河流域的鱼类资源进行长期监测,关注鱼类群落结构变化,一旦发现异常及时采取措施;增加对土著鱼类的增殖放流;严格控制进出口贸易和增殖放流时夹带的外来鱼类。维护我国伊犁河流域的生态安全。     

实验用鱼类福利的发展现状

伴随着鱼类作为科研重要材料的迅猛发展,实验用鱼类福利引起了人们的关注。本文旨在阐述国内外鱼类福利发展的历史和现状,剖析鱼类福利实施困难的因素,提供提高福利的参考对策,以期帮助科研工作者进一步地了解实验用鱼类福利,促进在实验过程中鱼类福利的发展。     

基于水声学频差技术的镇江长江豚类省级自然保护区鱼类资源时空特征

为掌握镇江长江豚类省级自然保护区鱼类资源现状,于2020年春季(4月)和秋季(9月)利用水声学频差技术和网具调查方法同步开展鱼类资源调查,评估调查江段鱼类资源时空特征。水声学调查结果表明,调查江段鱼类资源密度均值为(0.094±0.183)尾/m²,鱼类单体目标强度(Target strength, TS)均值为–(57.33±5.69) dB,主要分布在–62.5—–50.5 dB,随着水深的增加, TS逐渐增大;网具调查结果表明,调查江段共采集鱼类758尾、53.53 kg,鉴定鱼类种类达48种,其中以鲤形目(Cypriniformes)鱼类占优,中上层鱼类群体数量和质量占比分别为36.15%和66.12%,长江江豚饵料鱼类(体长小于20 cm、体高小于6 cm、体重小于100 g的中上层鱼类)数量和质量占比分别为23.22%和8.72%。在时间特征上, 4月和9月鱼类资源密度差异不显著(P>0.05), 4月鱼类TS均值小于9月,且4月鱼类平均规格小于9月。在空间特征上,鱼类资源主要分布于和畅洲北汊和焦北滩附近水域,中上层鱼类资源相对较多,且4月鱼类资...     

长江口中华鲟自然保护区潮间带鱼类群落结构

为深入了解鱼类对潮间带水域的利用,2010年5月、8月、11月在长江口中华鲟自然保护区潮间带水域进行了插网调查。结果表明:调查期间共出现28种鱼类,隶属于10目16科,鲤形目种类最多,其次为鲈形目;群落优势种为中国花鲈(Lateolabrax maculates)、鮻(Liza haematocheila)和窄体舌鳎(Cynoglossus gracilis);长江口中华鲟自然保护区潮间带鱼类群落可以分为河口定居鱼类、海洋偶见鱼类、降海洄游鱼类、溯河洄游鱼类、海洋洄游鱼类和淡水鱼类6种生态类群,其中河口定居鱼类和海洋洄游鱼类占据明显优势。对调查水域鱼类群落进行聚类分析,结果表明秋季和春夏季差异显著,鮻、中国花鲈、斑尾刺虾虎鱼(Acanthogobius ommaturus)是对季节差异贡献最大的3种鱼类。主成分分析结果表明,夏季鱼类群落主要受到海洋洄游鱼类的影响,而秋季主要受到河口定居鱼类的影响,这2种鱼类的幼鱼在不同季节利用潮间带水域索饵肥育可能是造成季节差异的主要原因。     

西沙东岛珊瑚礁鱼类种类组成特征与演变

为更好保护和管理东岛珊瑚礁鱼类资源, 2021和2022年通过水下摄影和水下潜水捕捞对东岛珊瑚礁鱼类进行现场调查,并结合历史研究探讨了东岛珊瑚礁鱼类种类组成及历史演变特征,结果如下:东岛共发现珊瑚礁鱼类235种,隶属于辐鳍鱼纲(Actinopterygii; 222种)和软骨鱼纲(Chondrichthyes; 13种),由16目、55科组成,其中鲈形目(Perciformes)鱼类最多,为181种,科层面,蝴蝶鱼科(Chaetodontidae)和鹦嘴鱼科(Scaridae)最多,均22种。东岛珊瑚礁鱼类物种数与其对应的最大全长成指数相关,随其增加而显著减少。东岛以小型鱼类最多,占总种类数的38.72%;其次是中型鱼类,为34.47%;大型鱼类为26.81%。从食性类型来看,东岛肉食性鱼类最多,占物种数的56.60%。各时间段之间鱼类的相似性指数均较低,证实了鱼类发生了显著的演替变化。研究较历史共有103种鱼类未发现,其中,肉食性鱼类占未发现鱼类的74.76%,同时未发现大型鱼类几乎全部分布在肉食性鱼类中; 14种位列于IUCN红色名录的鱼类,除一种外,其他均在此研究中未发现并均为大...     

鱼类生殖细胞移植的研究进展

生殖细胞移植在生物学、畜牧学及兴起的鱼类生物工程上都有很多用途。目前,采用基于基因组的育种方法已选育出多种带有所需遗传性状的鱼类新品系;但由于缺乏鱼卵与胚胎的低温保存技术,尚未找到长期保存其种质资源的方法。就鱼类生殖细胞移植的最新研究进展进行综述,同时针对鱼类生殖细胞移植及其低温保存技术研究中存在的问题进行讨论,并提出了鱼类生殖细胞移植的应用前景,以期能积极推动鱼类生物工程的研究,加速鱼类生殖细胞移植在产业中的应用。     

三峡水库蓄水后长江上游鱼类群聚沿河流-水库梯度的空间格局

研究对2005—2012年长江上游合江、木洞、万州和秭归江段的鱼类群聚结构进行了调查, 以分析三峡水库蓄水后长江上游鱼类群聚沿河流-水库梯度的空间格局。结果显示, 在三峡蓄水后, 在合江至秭归江段累计采集到土著鱼类368706尾, 合计132种, 隶属于17科, 其中鲤科鱼类为优势类群。沿河流-水库纵向梯度, 土著鱼类物种数下降而外来鱼类物种数增加。对应分析表明, 合江至秭归江段的鱼类群聚呈现出明显分化: 库区以上河段鱼类组成以流水性鱼类为主, 库区鱼类则以静水缓流型为主。指示物种分析进一步指出, 河流区鱼类以犁头鳅(Lepturichthys fimbriata)、红唇薄鳅(Leptobotia rubrilabris)、圆口铜鱼(Coreius guichenoti)、圆筒吻(Rhinogobio cylindricus)等22种鱼类为指示物种, 其功能群特征表现为偏好流水生境、生活史为均衡主义及食性为昆虫食性; 库区鱼类以宽口光唇鱼(Acrossocheilus monticolus)、宽鳍鱲(Zacco platypus)、鳊(Parabramis pekinensis)、鳙(Aristichthys nobilis)等16种鱼类为指示物种, 其功能群特征表现为偏好静水生境、食性为草食性或浮游食性。以上研究表明, 三峡水库蓄水导致的水环境变化是影响长江上游鱼类纵向格局的主要驱动力。建议相关管理部门根据不同河段鱼类群聚特征制定不同的渔业管理措施, 如保护土著鱼类资源、控制外来入侵鱼类。     

秦岭西段鱼类多样性现状初报

对秦岭西段汉江水系和嘉陵江水系的鱼类和环境调查显示,人类活动对鱼类多样性产生了较大的影响。本文试图对该地区鱼类多样性丧失的现状加以报道并对原因进行分析。以前该地区鱼类共有142种,本次调查的渔获物中只有34种。鱼类物种资源的减少是多种因素造成的：水坝的修建使得鱼类栖息地环境改变从而导致鱼类的种类和种群数量变化,水污染、过度捕捞和非法渔业也在一定程度上破坏了鱼类物种多样性。     

Fishborne Trematode Metacercariae in Luang Prabang,Khammouane, and Saravane Province,Lao PDR

Fishborne trematode (FBT) metacercariae were investigated in fish from 3 Provinces of Lao PDR. Total 242 freshwater fish of 40 species were collected in local markets of Luang Prabang (59 fish of 16 species), Khammouane (81 fish of 19 species), and Saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. Four species of metacercariae (Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, and Centrocestus formosanus) were detected. O. viverrini was detected in 35 fish (14.5%), and their density was 252 per infected fish (Luang Prabang, 88 metacercariae in 5 fish; Khammouane, 187 in 6 fish; Saravane, 303 in 24 fish). H. taichui was found in 102 fish (42.1%), and their density was 485 per infected fish (Luang Prabang, 260 metacercariae in 38 fish; Khammouane, 1,084 in 23 fish; Saravane, 359 in 41 fish). H. yokogawai was detected in 92 fish (38.0%), and their density was 222 per infected fish (Luang Prabang, 362 metacercariae in 17 fish; Khammouane, 126 in 20 fish; Saravane, 214 in 55 fish). Metacercariae of C. formosanus were found in 8 fish (3.3%), and their density was 3 per infected fish. In the present study, it has been confirmed that FBT metacercariae, in particular, H. taichui, H. yokogawai, and O. viverrini, are highly prevalent in fish from Luang Prabang, Khammouane, and Saravane Province, Lao PDR.     

Fishborne Trematode Metacercariae in Freshwater Fish from Guangxi Zhuang Autonomous Region, China

A survey was performed to investigate the infection status of fishborne trematode (FBT) metacercariae in freshwater fish from Guangxi Zhuang Autonomous Region, China. A total of 307 freshwater fish of 31 species were collected from 5 administrative regions of Guangxi Zhuang Autonomous Region. They were examined by artificial digestion method from July 2003 to August 2004. No metacercariae were detected in fish from Fusui-xian. In fish from Mashan-xian and a market in Nanning, 3 species of metacercariae, Haplorchis taichui, Haplorchis pumilio, and Centrocestus formosanus, were mainly detected. Metacercariae (8 in number) of Clonorchis sinensis were found in 1 Chanodichthys dabryi purchased from a market in Nanning. In fish from Yangshuo, Metagonimus yokogawai metacercariae were detected from all 18 fish species examined. Total 13 C. sinensis metacercariae were found in 3 out of 10 Hemibarbus maculatus from Yangshuo. All 7 Zacco platypus from Yangshuo were infected with 8-112 Echinochasmus perfoliatus metacercariae. In fish from Binyang-xian, H. pumilo metacercariae were mainly detected in all 5 fish species examined, and only 1 metacercaria of C. sinensis was found in a Hemiculter leucisculus. From the above results, it was confirmed that some species of freshwater fish play a role of second intermediate hosts for FBT in Guangxi Zhuang Autonomous Region, China. In particular, 4 species of intestinal flukes, M. yokogawai, H. taichui, H. pumilio, and C. formosanus, were prevalent in fish hosts, whereas C. sinensis metacercariae were detected only in 3 fish species.     

Zoonotic Trematode Metacercariae in Fish from Phnom Penh and Pursat,Cambodia

A survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in Phnom Penh and Pursat Province, Cambodia. All collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. In fish from Phnom Penh, 2 kinds of metacercariae (Opisthorchis viverrini and Haplorchis yokogawai) were detected. O. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fish, 18.6). H. yokogawai metacercariae were detected in 23 (57.5%) of 40 fish in 5 species (average no. metacercariae/fish, 21.0). In fish from Pursat Province, 5 kinds of metacercariae (O. viverrini, H. yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum sp.) were detected; O. viverrini metacercariae (n=3) in 2 fish species (Henicorhynchus lineatus and Puntioplites falcifer), H. yokogawai metacercariae (n=51) in 1 species (P. falcifer), H. pumilio metacercariae (n=476) in 2 species (H. lineatus and Pristolepis fasciata), C. formosanus metacercariae (n=1) in 1 species (H. lineatus), and Procerovum sp. metacercariae (n=63) in 1 species (Anabas testudineus). From the above results, it has been confirmed that various freshwater fish play the role of a second intermediate host for zoonotic trematodes (O. viverrini, H. yokogawai, H. pumilio, C. formosanus, and Procerovum sp.) in Cambodia.     

Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics

Malacosporeans represent a small fraction of myxozoan biodiversity with only two genera and three species described. They cycle between bryozoans and freshwater fish. In this study, we (i) microscopically examine and screen different freshwater/marine fish species from various geographic locations and habitats for the presence of malacosporeans using PCR; (ii) study the morphology, prevalence, host species/habitat preference and distribution of malacosporeans; (iii) perform small subunit/large subunit rDNA and Elongation factor 2 based phylogenetic analyses of newly gathered data, together with all available malacosporean data in GenBank; and (iv) investigate the evolutionary trends of malacosporeans by mapping the morphology of bryozoan-related stages, host species, habitat and geographic data on the small subunit rDNA-based phylogenetic tree. We reveal a high prevalence and diversity of malacosporeans in several fish hosts in European freshwater habitats by adding five new species of Buddenbrockia and Tetracapsuloides from cyprinid and perciform fishes. Comprehensive phylogenetic analyses revealed that, apart from Buddenbrockia and Tetracapsuloides clades, a novel malacosporean lineage (likely a new genus) exists. The fish host species spectrum was extended for Buddenbrockia plumatellae and Buddenbrockia sp. 2. Co-infections of up to three malacosporean species were found in individual fish. The significant increase in malacosporean species richness revealed in the present study points to a hidden biodiversity in this parasite group. This is most probably due to the cryptic nature of malacosporean sporogonic and presporogonic stages and mostly asymptomatic infections in the fish hosts. The potential existence of malacosporean life cycles in the marine environment as well as the evolution of worm- and sac-like morphology is discussed. This study improves the understanding of the biodiversity, prevalence, distribution, habitat and host preference of malacosporeans and unveils their evolutionary trends.     

Prevalence of Zoonotic Trematode Metacercariae in Freshwater Fish from Gangwon-do,Korea

The infection status of zoonotic trematode metacercariae was investigated in a total of 2,293 freshwater fish collected from 11 rivers or streams in 9 administrative regions of Gangwon-do, Korea for 5 years (2009-2013). All fish were collected by netting methods and examined using the artificial digestion methods. Clonorchis sinensis metacercariae were detected in 4 fish species, i.e., Pungtungia herzi, Squalidus japonicus coreanus, Acheilognathus rhombeus, and Ladislabia taczanowskii, from only Hantangang in Cheorwon-gun. Metagonimus spp. metacercariae were found in 1,154 (50.3%) fish and their average number per infected fish was 55.8. Among the positive fish species, especially Tribolodon hakonensis from Namdaecheon in Yangyang-gun and Plecoglossus altivelis from Osipcheon in Samcheok-si were most heavily infected. Centrocestus armatus metacercariae were detected in 611 (26.7%) fish and the average metacercarial burden per infected fish was 1,032. Two chub species, Zacco platypus and Zacco temminckii were highly and heavily infected with C. armatus metacercariae in almost all regions surveyed. Echinostoma spp. metacercariae were also found in 24 fish from a few localities, but their numbers per fish infected were very low. From the above results, it is confirmed that the metacercariae of intestinal flukes, especially Metagonimus spp. and C. armatus, were heavily infected, while C. sinensis metacercariae were rarely found in fish from Gangwon-do, Korea.     

Fishborne Trematode Metacercariae Detected in Freshwater Fish from Vientiane Municipality and Savannakhet Province, Lao PDR

Freshwater fish from Vientiane Municipality and Savannakhet Province, Lao PDR were examined by the muscle compression and artificial digestion methods to know the infection status with trematode metacercariae. In the fish from Savannakhet, 2 species of metacercariae, Opisthorchis viverrini and Haplorchis taichui, were detected. O. viverrini metacercariae were found in 6 species of fish, Puntius brevis, Hampala dispar, Esomus metallicus, Mystacoleucus marginatus, Puntioplites falcifer, and Cyclocheilichthys armatus. H. taichui metacercariae were detected in 3 species of fish, P. brevis, P. falcifer, and M. marginatus. In the fish from Vientiane, 4 species of metacercariae, O. viverrini, H. taichui, Haplorchis yokogawai, and Centrocestus formosanus, were detected. Among them, O. viverrini metacercariae were found in 7 species of fish, Onychostoma elongatum, C. armatus, H. dispar, P. brevis, Cyclocheilichthys repasson, Osteochilus hasseltii, and Hypsibarbus lagleri. The metacercariae of H. taichui were detected in 6 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, and Cirrhinus molitorella. The metacercariae of H. yokogawai were found in 9 species of fish, C. repasson, O. elongatum, C. armatus, H. dispar, Labiobarbus leptocheila, O. hasseltii. C. molitorella, Hypsibarbus wetmorei, and H. lagleri. The metacercariae of C. formosanus were detected in 4 species of fish, C. repasson, P. brevis, O. hasseltii, and C. molitorella. From these results, it is confirmed that fishborne trematode metacercariae, i.e. O. viverrini, H. taichui, H. yokogawai and C. formosanus, are prevalent in various species of freshwater fish from Savannakhet Province and Vientiane Municipality, Lao PDR.     

One known and an unknown species of the genus Thaparocleidus Jain, 1952, infecting Sperata aor (Hamilton, 1822): comparison with species from China,on molecular basis

Sperata aor commonly called as long- whiskered cat fish or “Bada Tengan” in local fish markets harboured one new and one previously known species of genus Thaparocleidus Jain, 1952, along with two species of Cornudiscoides Kulkarni, 1969, infesting gills. Thaparocleidus aori (Rizvi, 1971) Lim, 1996, was earlier described by Rizvi therefore was briefly recorded in the present study, except the egg. The newly found species Thaparocleidus susanae n.sp was characterized by the structure of its peculiar copulatory organ. Phylogenetic relationship of the two species under study, along with 14, reterived from GenBank was established using the sequences of 28S rDNA region (Dactylogyrus Diesing, 1850 taken as an out group).     

Foodborne Trematode Metacercariae in Fish from Northern Vietnam and Their Adults Recovered from Experimental Hamsters

The prevalence of foodborne trematode (FBT) metacercariae was investigated in fish from 2 localities of northern Vietnam in 2004-2005. Freshwater fish (9 species) were collected from local markets in Hanoi City (n=76) and Nam Dinh Province (n=79), and were examined for FBT metacercariae using the artificial digestion technique. Adult flukes were obtained from hamsters experimentally infected with the metacercariae at day 8 post-infection. Three (Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium) and 6 (Haplorchis taichui, H. pumilio, C. formosanus, P. varium, Stellantchasmus falcatus, and Heterophyopsis continua) species of FBT metacercariae were detected in the 2 regions, respectively. Overall, among the positive fish species, H. pumilio metacercariae were detected in 104 (80.0%) of 130 fish examined (metacercarial density per infected fish; 64.2). C. formosanus metacercariae were found in 37 (40.2%) of 92 fish (metacercarial density; 14.7). P. varium metacercariae were detected in 19 (63.3%) of 30 fish (Anabas testudineus and Mugil cephalus) (metacercarial density; 247.7). S. falcatus metacercariae were found in all 10 M. cephalus examined (metacercarial density; 84.4). H. continua metacercariae (2 in number) were detected in 1 fish of Coilia lindmani. Morphologic characteristics of the FBT metacercariae and their experimentally obtained adults were described. The results have demonstrated that various FBT species are prevalent in northen parts of Vietnam.     

Higher parasite richness, abundance and impact in native versus introduced cichlid fishes

Empirical studies suggest that most exotic species have fewer parasite species in their introduced range relative to their native range. However, it is less clear how, ecologically, the loss of parasite species translates into a measurable advantage for invaders relative to native species in the new community. We compared parasitism at three levels (species richness, abundance and impact) for a pair of native and introduced cichlid fishes which compete for resources in the Panama Canal watershed. The introduced Nile tilapia, Oreochromis niloticus, was infected by a single parasite species from its native range, but shared eight native parasite species with the native Vieja maculicauda. Despite acquiring new parasites in its introduced range, O. niloticus had both lower parasite species richness and lower parasite abundance compared with its native competitor. There was also a significant negative association between parasite load (abundance per individual fish) and host condition for the native fish, but no such association for the invader. The effects of parasites on the native fish varied across sites and types of parasites, suggesting that release from parasites may benefit the invader, but that the magnitude of release may depend upon interactions between the host, parasites and the environment.     

Molecular Analysis of Anisakis Type I Larvae in Marine Fish from Three Different Sea Areas in Korea

Anisakiasis, a human infection of Anisakis L3 larvae, is one of the common foodborne parasitic diseases in Korea. Studies on the identification of anisakid larvae have been performed in the country, but most of them have been focused on morphological identification of the larvae. In this study, we analyzed the molecular characteristics of 174 Anisakis type I larvae collected from 10 species of fish caught in 3 different sea areas in Korea. PCR-RFLP and sequence analyses of rDNA ITS and mtDNA cox1 revealed that the larvae showed interesting distribution patterns depending on fish species and geographical locations. Anisakis pegreffii was predominant in fish from the Yellow Sea and the South Sea. Meanwhile, both A. pegreffii and A. simplex sensu stricto (A. simplex s.str.) larvae were identified in fish from the East Sea, depending on fish species infected. These results suggested that A. pegreffii was primarily distributed in a diverse species of fish in 3 sea areas around Korea, but A. simplex s.str. was dominantly identified in Oncorhynchus spp. in the East Sea.