中国兽类新纪录──戈壁阿尔泰高山鼠

中国兽类新纪录──戈壁阿尔泰高山鼠ＮＥＷＲＥＣＯＲＤＯＦＭＡＭＭＡＬＳＩＮＣＨＩＮＡ──ＡＬＴＩＣＯＬＡＢＡＲＡＫＳＨＩＮＫｅｙｗｏｒｄｓＣｈｉｎａ;Ｎｅｗｒｅｃｏｒｄ;Ｍｏｕｎｔａｉｎｖｏｌｅ;作者于１９９３年８月２６－２７日在新疆巴里坤县中蒙边境...     

巧遇野骆驼

1995年至1999年,我代表新疆环境保护科学研究所与联合国环境规划署官员简·海尔(John Hare)博士组织的考察队,在我国4个野双峰驼分布区进行了合作科学考察。五年中6次进入罗布泊无人区,考察中克服了遇到的多次危险和困难,规划建立了阿尔金山—罗布泊野双峰驼自然保护区,站卡控制面积达15万平方公里,在国际组织的支持下实施了对该野驼模式产地的保护。下面是我与野双峰驼相遇的亲身经历。 1995年3月底,我们经过15天跋涉,行程1500公里,穿越了罗布泊北部戛顺戈壁无人区,只找到了野驼的一些新鲜粪便和足迹,但未能见到一头野驼。在中蒙边境考察中也使我们大失所望。5月初,我们又来到罗布泊南部的阿尔金山北麓拉配泉。 在泉水池边,看到了野驴、野骆驼及盘羊的一些蹄印,但不多。我想在这十多     

额尔齐斯河高体雅罗鱼单殖吸虫中国二新纪录种

额尔齐斯河(Ergis River)发源于中蒙边境,地处欧亚大陆腹部,上游在我国境内,位于新疆阿尔泰地区东北部,长为500 km,流域面积50000 km2,是新疆三大河流之一,也是我国唯一一条北冰洋水系的国际河流。       

黄羊（Procapra gutturosa）在中国分布的变迁及其资源持续利用

历史上黄羊曾广布于中国的北部,目前黄羊只分布在我国内蒙古北部的中蒙边境一带。环境的变迁、人类活动的干扰及盲目捕猎是黄羊分布区缩小的重要原因。建议尽快建立黄羊自然保护区,使黄羊在我国有一安歇、繁衍的良好栖息地,最终使黄羊资源得到科学合理的利用。     

阿拉善高原拟步甲的多样性与区系组成

正拟步甲科昆虫对环境的适应能力极强,在全球广泛分布,目前已知约20,000种,隶属于9亚科97族,约2,300属(Matthews et al,2010;Bouchard et al,2011)。阿拉善高原位于贺兰山以西、甘肃新疆边界以东的沙漠、戈壁集中分布区;北至中蒙国界线,东至贺兰山,西至马鬃山地东端,南部边界以阿拉善地台边缘为界,包括龙首山、合黎山,其南缘邻接     

内蒙古狼山北部荒漠地区哺乳动物区系的初步分析

1978年夏季对狼山北部荒漠地区,进行了初步调查。 狼山北部为内蒙古自治区巴彦诺尔盟潮格旗境内西北部的砾质荒漠。北面与蒙古人民共和国南戈壁毗邻,西面与阿拉善左旗接壤,东南部则逐渐过渡到狼山北部草原化荒漠地带,北纬41°20′—43°30′,东经105°00′—107°30′之间。地势由西北向东南逐渐上升,中蒙边境一带     

气候变暖背景下新疆和中哈边境亚洲飞蝗适生区预测及变化

亚洲飞蝗Locusta migratoria migratoria是中国新疆与哈萨克斯坦边境区域重要害虫之一,具迁飞性且繁殖能力强,对农牧业经济造成巨大伤害。本文以亚洲飞蝗为研究对象,根据其发生区和相关生物学特性,利用CLIMEX 4.0.2和ArcGIS 10.2相结合,预测其在新疆和中哈边境区域亚洲飞蝗的潜在地理分布。亚洲飞蝗的适生区在新疆境内集中分布在北疆（42.69~48.29°N）,包括伊犁、博尔塔拉中部、塔城西北部及南部、阿勒泰西部、昌吉和乌鲁木齐南部地区;哈萨克斯坦境内主要分布在北纬47.03~51.65°N区域,包括东哈萨克斯坦州北部、阿拉木图州东南部境内的湖泊及河流沿岸地区,这与亚洲飞蝗的现有分布点和历史发生记录吻合。未来气候变暖情景下,亚洲飞蝗在中哈边境新疆境内的适生区逐渐扩大,其中在阿勒泰、伊犁、昌吉及乌鲁木齐的高度适生区面积增加,哈密开始出现高度适生区;在哈萨克斯坦边境区域的适生区略有增加,其中东哈萨克斯坦州地区高度适生区面积减少明显。研究结果对区域内亚洲飞蝗的预测预报和综合防控具有重要意义。     

中蒙俄经济走廊荒漠化时空格局变化及其驱动因子

针对如何定量揭示中蒙俄经济走廊荒漠化的时空变化规律及其驱动因子的影响作用,在构建荒漠化程度判别标准和指标体系的基础上,基于Google Earth Engine云计算平台,在对分类回归树(CART)、支持向量机(SVM)、随机森林(RF)和Albedo-NDVI 4个模型精度进行对比分析的基础上,选取精度最高的CART模型实现中蒙俄经济走廊区域2000—2015年的荒漠化的时空变化分析。另外,在拓展线性趋势法的基础上,构建荒漠化驱动因子定量评估模型,实现气候变化和人类活动对荒漠化驱动效应的定量测度。结果表明:1)CART模型在中蒙俄经济走廊区域荒漠化信息提取精度最高,总体精度和Kappa系数分别85%和0.754;2)极重度荒漠化区域主要分布在中国内蒙古自治区西部,蒙古国的南戈壁盟、东戈壁盟和中戈壁盟;3)2000—2015年间,中蒙俄经济走廊荒漠化总体呈扩张趋势,但极重度、重度和中度荒漠化面积呈缩减趋势。其中,中国的荒漠化面积呈明显缩减趋势,荒漠化程度净改善面积为15.18万km~2,而蒙古国和俄罗斯的荒漠化现象在加重。另外,中蒙俄经济走廊荒漠化恢复区域的气候变化驱动贡献率为68.8%;而荒漠化退化区域的人类活动驱动贡献率为69.68%,表明中蒙俄经济走廊荒漠化程度加重区域的驱动作用主要来自于人类活动。     

赤颊黄鼠的生态初步调查

赤颊黄鼠(Citellus erythrogenus)在我国仅分布于内蒙古和新疆。在内蒙古它是中温型荒漠草原亚带植被的代表鼠种之一,主要沿北部(东苏、西苏、二连,四子王,达茂、乌拉特中后联合旗)中蒙边境一带,呈条带状均匀分布。有关它的生物学特性,在国外虽已有一些研究,(见1954,1965等),但在我国还未见报道。     

蒙古高原天牛亚科昆虫种类组成及区系分析

对蒙古高原天牛亚科昆虫种类组成及区系进行初步分析,已记载共26属69种,各属所包含的天牛种类数量差异很大,以Xylotrechus为优势属,Chlorophorus次之,且在俄、蒙、中三国,各属的种类组成、数量也有差异;在种的组成中,以古北种所占比例最大,为60.87%,广布种也占有相当比例为39.13%。Xylotrechus在俄、蒙、中三国均以古北种占优势,而Chlorophorus在蒙古国以古北种占优势,在俄、中两国以广布种占优势。柽柳漠天牛Hesperophanes heydeni Baeckm.为中国新记录种。中国无分布记录的蒙古高原天牛亚科昆虫有4种:戈壁鞍背天牛Asias gobiensis Namhaidorzh、乌布苏虎天牛Chlorophorus ubsanurensis Tsherepanov、蒙瘦尾鞘天牛Leptepania okunevi Shabliovsky、阿木尔脊虎天牛Xylotrechus pantherinus(Sav.)。     

罗布泊野骆驼的家域特征及其意义

野双峰骆驼(Camelus ferus)生活在荒漠戈壁, 种群数量稀少, 栖息地地形复杂多样, 且有长距离迁徙习性, 目前对其家域面积和重要栖息地范围的研究只有定性描述。本研究于2012年5月至2013年7月, 在阿奇克谷地和阿尔金山北麓捕捉了8峰野骆驼并安装GPS卫星跟踪项圈, 分别获得了12-423天的13,748个GPS位点记录。研究结果表明, 8峰野骆驼的100%最小凸多边形(minimum convex polygons, MCP)家域面积分别为1,775-11,768 km², 总家域面积为32,821 km², 占罗布泊野骆驼分布区面积的23.1%, 平均家域面积为7,349 ± 1,323 km²。野骆驼个体家域面积季节间差异显著, 秋季最大, 其次是冬季和夏季, 春季最小, 秋季是春季的4.4倍。野骆驼在繁殖季节的家域平均面积为879 ± 320 km², 非繁殖季节为998 ± 106 km², 二者间无显著差异。除了项圈编码为135号的野骆驼和其他7峰野骆驼的家域没有重叠外, 其余7峰野骆驼的家域都有重叠。这7峰野骆驼的家域总面积是24,910 km², 占罗布泊野骆驼分布区总面积的17.5%, 重叠区面积是515 km²。鉴于野骆驼主要分布在阿奇克谷地及以南区域、阿尔金山北麓, 建议将罗布泊野骆驼国家级自然保护区位于阿奇克谷地北山以北的核心区部分区域、磁海低地以南的山区和阿尔金山西部原为实验区的部分区域调整为缓冲区, 而将阿尔金山北麓至库姆塔格沙漠之间的戈壁地带原为缓冲区的部分区域调整为核心区。     

High mitochondrial differentiation levels between wild and domestic Bactrian camels: a basis for rapid detection of maternal hybridization

Hybridization between wild species and their domestic congeners often threatens the gene pool of the wild species. The last wild Bactrian camel (Camelus ferus) populations in Mongolia and China are examples of populations facing such a hybridization threat. To address this key issue in the conservation of wild camels, we analysed wild, hybrid and domestic Bactrian camels (Camelus bactrianus) originating from Mongolia, China and Austria. Through screening of an 804‐base‐pair mitochondrial fragment, we identified eight mitochondrial haplotypes and found high sequence divergence (1.9%) between C. ferus and C. bactrianus. On the basis of a mitochondrial DNA sequence fixed difference, we developed a diagnostic PCR restriction fragment length polymorphism (PCR‐RFLP) assay to differentiate between wild and domestic camel samples. We applied the assay to 81 individuals and confirmed the origin of all samples including five hybrids with known maternal ancestry. The PCR‐RFLP system was effective for both traditional (blood, skin) and non‐invasive samples (faeces, hair), as well as for museum specimens. Our results demonstrate high levels of mitochondrial differentiation between wild and domestic Bactrian camels and that maternal hybridization can be detected by a rapid and reliable PCR‐RFLP system.     

Genetic diversity and phylogeographic structure of Bactrian camels shown by mitochondrial sequence variations

The Bactrian camel includes various domestic (Camelus bactrianus) and wild (Camelus ferus) breeds that are important for transportation and for their nutritional value. However, there is a lack of extensive information on their genetic diversity and phylogeographic structure. Here, we studied these parameters by examining an 809‐bp mtDNA fragment from 113 individuals, representing 11 domestic breeds, one wild breed and two hybrid individuals. We found 15 different haplotypes, and the phylogenetic analysis suggests that domestic and wild Bactrian camels have two distinct lineages. The analysis of molecular variance placed most of the genetic variance (90.14%, P < 0.01) between wild and domestic camel lineages, suggesting that domestic and wild Bactrian camel do not have the same maternal origin. The analysis of domestic Bactrian camels from different geographical locations found there was no significant genetic divergence in China, Russia and Mongolia. This suggests a strong gene flow due to wide movement of domestic Bactrian camels.     

近二十年来罗布泊地区生态环境研究综述

随着近期我国"一带一路"生态文明建设以及生态文明建设的提出与实施,生态类型丰富但自然环境脆弱的西北地区再度引发学界关注。历史上罗布泊地区作为陆上丝绸之路交通重要节点,在中外文明交往与民族融合进程中发挥着重大作用,但如今罗布泊干涸以及罗布泊地区生态环境恶化的现实促使学界对该区域的生态环境演变历史进行反思。为进一步了解当前罗布泊地区生态环境研究的整体概况、热点议题及学术前沿趋势,从自然环境与人文环境两大方面对近二十年来罗布泊地区生态环境研究做出阶段性总结,以期为后续深入探讨区域生态环境变化与社会文明演进互动提供参考。     

Microscopic and molecular detection of Deraiophoronema evansi (Lewis, 1882) in domestic Bactrian camels (Camelus bactrianus) of Mongolia

Cameline filarosis is an important parasitic disease having an economic impact on the camel industry around the world. However, there has been no study on filarosis in Bactrian camels of Mongolia. Therefore, the aim of the present study was to detect and identify microfilariae of Deraiophoronema evansi (D. evansi) in Bactrian camels from three provinces, located in southern and southwestern Mongolia. Blood samples were obtained from 400 healthy two-humped camels of different ages and both sexes. All blood samples were analysed using a variety of diagnostic techniques. Microfilariae were detected in 30 Bactrian camels (7.5%) by the Knott technique, while 13 Bactrian camels (3.3%) tested positive in a direct smear test. D. evansi was detected in 18 Bactrian camels (4.5%) by PCR assay. Prevalence was shown to be high among Bactrian camels in the age group up to 5 years, while the lowest positive results were obtained for Bactrian camels in the 5–10-year age group and the over 10-year age group. To confirm the morphological identification, D. evansi–COI gene sequences were subjected to phylogenetic analyses. The D. evansi–COI gene sequences from Mongolian two-humped camels were identical to sequences from Iranian one-humped camels and were clustered together with these sequences in the phylogeny. This is the first report of molecular detection and identification of microfilariae of D. evansi in Bactrian camels of Mongolia.     

Characterization of heavy-chain antibody gene repertoires in Bactrian camels

Camelids are the only mammals that can produce functional heavy-chain antibodies (HCAbs). Although HCAbs were discovered over 30 years ago, the antibody gene repertoire of Bactrian camels remains largely underexplored. To characterize the diversity of variable genes of HCAbs (VHHs), germline and rearranged VHH repertoires are constructed. Phylogenetics analysis shows that all camelid VHH genes are derived from a common ancestor and the nucleotide diversity of VHHs is similar across all camelid species. While species-specific hallmark sites are identified, the non-canonical cysteines specific to VHHs are distinct in Bactrian camels and dromedaries compared with alpacas. Though low divergence at the germline repertoire between wild and domestic Bactrian camels, higher expression of VHHs is observed in some wild Bactrian camels than that of domestic ones. This study not only adds our understanding of VHH repertoire diversity across camelids, but also provides useful resources for HCAb engineering.     

Population genetic analysis of the domestic Bactrian camel in China by RAD‐seq

Restriction site‐associated DNA sequencing (RAD‐seq) is one of the most effective high‐throughput sequencing technologies for SNP development and utilization and has been applied to studying the origin and evolution of various species. The domestic Bactrian camels play an important role in economic trade and cultural construction. They are precious species resources and indispensable animals in China's agricultural production. Recently, the rapid development of modern transportation and agriculture, and the deterioration of the environment have led to a sharp decline in the number of camels. Although there have been some reports on the evolution history of the domestic Bactrian camel in China, the origin, evolutionary relationship, and genetic diversity of the camels are unclear due to the limitations of sample size and sequencing technology. Therefore, 47 samples of seven domestic Bactrian camel species from four regions (Inner Mongolia, Gansu, Qinghai, and Xinjiang) were prepared for RAD‐seq analysis to study the evolutionary relationship and genetic diversity. In addition, seven domestic Bactrian camel species are located in different ecological zones, forming different characteristics and having potential development value. A total of 6,487,849 SNPs were genotyped. On the one hand, the filtered SNP information was used to conduct polymorphism mapping construction, LD attenuation analysis, and nucleotide diversity analysis. The results showed that the number of SNPs in Dongjiang camel was the highest, the LD coefficient decayed the fastest, and the nucleotide diversity was the highest. It indicates that Dongjiang camel has the highest genetic diversity. On the other hand, the filtered SNPs information was used to construct the phylogenetic tree, and F_ST analysis, inbreeding coefficient analysis, principal component analysis, and population structure analysis were carried out. The results showed that Nanjiang camel and Beijiang camels grouped together, and the other five Bactrian camel populations gathered into another branch. It may be because the mountains in the northern part of Xinjiang and the desert in the middle isolate the two groups from the other five groups.     

利用卫星追踪颈圈对圈养野生双峰驼野化放归的监测

2012-2014年,通过对甘肃敦煌西湖国家级自然保护区野化放归的两峰野生双峰驼（Camelus ferus）佩带卫星追踪颈圈进行跟踪监测,利用最小凸多边形法和内核法开展了放归生境中野骆驼的活动范围和空间利用研究。研究期间,分别进行了22个月(ID:108444)和9个月(ID:108445)的跟踪监测,获得了3403个(ID:108444) 和1573个(ID:108445)定位成功的GPS位点。野骆驼放归后的前两个月仍存在一定的对圈养环境的依赖,两个月后野骆驼的活动范围开始向外扩展,直至四个月后,基本扩展至整个放养的围栏,活动范围分别从9.5109 km²增加到19.3694 km2 (ID:108444),8.8943 km² 增加到19.4192 km² (ID:108445)。整个监测期间,95% Kernel活动范围分别为7.7181km² (ID:108444) 和 3.0321 km² (ID:108445)。从整个监测期间(ID:108444)的50% Kernel活动范围（0.2811 km²）来看,野骆驼整个放归期间仍然比较依赖原先的圈养环境;野骆驼存在对胡杨疏林的偏好;同时,放归的野骆驼仍然保持了对人的亲近行为。从2013年整年来看,放归野骆驼实际生境利用,春、秋两季范围大,夏、冬两季范围较小。放归的野骆驼不同季节对主要生境的利用也有所不同,最为明显的是夏季,野骆驼活动范围集中在荒漠地区,避开植被多的区域以避免蚊虫叮咬。本研究进一步了解了野骆驼的行为习性及其适应环境的行为对策,为野骆驼圈养种群的科学管理和进一步野化放归提供了理论依据。     

新疆罗布泊地区可培养嗜盐古菌多样性及其功能酶筛选

【目的】探索新疆罗布泊地区高盐环境可培养嗜盐古菌的多样性及其功能酶应用潜力。【方法】采集罗布泊地区13份土样,用纯培养并结合基于16S rRNA基因系统发育分析的方法来研究样品中嗜盐古菌的多样性。按系统进化树的聚类关系,挑选出一些菌株进行盐度耐受及淀粉酶、蛋白酶、酯酶的酶活检测。【结果】从13份土样中共分离到56株嗜盐古菌,经16S rRNA基因克隆测序,通过MEGA 4.0构建N-J树分析,56株菌分布于嗜盐古菌的10个生效发表属和5个潜在新属。运用Shannon-Wiener方法计算其多样性指数为1.820。挑选17株嗜盐古菌所测试盐浓度实验结果表明这一批嗜盐古菌的大部分生长范围在10%-35%之间,最适盐浓度在20%-25%之间。不同酶活检测结果为:淀粉酶酶活率为70.6%,蛋白酶酶活率为35.3%,酯酶酶活率为82.4%。【结论】新疆罗布泊周边地区由于气候及地理位置的独特性,蕴藏丰富的嗜盐古菌资源。本实验所设计的分离方法对嗜盐古菌的分离是极其有效的,为进一步研究新疆罗布泊及周边地区嗜盐古菌资源提供了技术基础。盐度耐受实验结果验证在低盐环境中分离嗜盐古菌新物种的可行性。同时,嗜盐古菌的酶活比率较高且活性较强为进一步开发利用嗜盐古菌资源提供了理论依据。     

罗布泊地区第四纪环境演化

新疆罗后干涸湖盆中的Ｋ１钻孔揭示,１００、２ｍ的沉积剖面共有９２个宙积层,划归１６同积旋回,包括了早更新世以来的各时期沉积。据划分的１５个孢粉组这及古地磁、１４＾Ｃ、岩石地层资料综合分析,早更新世该区曾发育森林－草原植被,早更民末,强烈的构造运动保留我下更充变形,上覆的中更统与下更新统间呈不整合接触。中更新世,荒漠和荒漠草原交替出现,地层中有大量膏质泥岩和石膏。晚更新世以来,在干旱气候的控制之下,