共查询到20条相似文献,搜索用时 31 毫秒
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K J McNamara-Schroeder R F Hennessey G A Harding R C Jensen W E Stumph 《The Journal of biological chemistry》2001,276(34):31786-31792
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Small nuclear RNA genes transcribed by either RNA polymerase II or RNA polymerase III in monocot plants share three promoter elements and use a strategy to regulate gene expression different from that used by their dicot plant counterparts. 总被引:2,自引:0,他引:2 下载免费PDF全文
S Connelly C Marshallsay D Leader J W Brown W Filipowicz 《Molecular and cellular biology》1994,14(9):5910-5919
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Christopher Marshallsay Sheila Connelly Witold Filipowicz 《Plant molecular biology》1992,19(6):973-983
We have demonstrated recently that the genes encoding the U3 small nuclear RNA (snRNA) in dicot plants are transcribed by RNA polymerase III (pol III), and not RNA polymerase II (pol II) as in all other organisms studied to date. The U3 gene was the first example of a gene transcribed by different polymerases in different organisms. Based on phylogenetic arguments we proposed that a polymerase specificity change of the U3 snRNA gene promoter occurred during plant evolution. To map such an event we are examining the U3 gene polymerase specificity in other plant species. We report here the characterization of a U3 gene from wheat, a monocot plant. This gene contains the conserved promoter elements, USE and TATA, in a pol III-specific spacing seen also in a wheat U6 snRNA gene characterized in this report. Both the U3 and the U6 genes possess typical pol III termination signals but lack the cis element, responsible for 3-end formation, found in all plant pol II-specific snRNA genes. In addition, expression of the U3 gene in transfected maize protoplasts is less sensitive to -amanitin than a pol II-transcribed U2 gene. Based on these data we conclude that the wheat U3 gene is transcribed by pol III. This observation suggests that the postulated RNA polymerase specificity switch of the U3 gene took place prior to the divergence of angiosperm plants into monocots and dicots. 相似文献
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Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes 总被引:1,自引:0,他引:1
Xia Li Dan-Hua Jiang Kelan Yong Da-Bing Zhang 《植物学报(英文版)》2007,49(2):222-229