Notes on fungi from north-east India — XXII some species of hypoxylon from Assam

Summary Hypoxylon stygium (Lév)Sacc.,H. archeri Berk.,H. bovei Speg. var.microspora Miller,H. truncatum (Schw.ex Fr.)Miller,H. nummularium Bull. ex Fr. var.merillii Miller (=Hypoxylon asarcodes (Theissen)Miller?) andH. deustum (Hoff. ex Fr.)Grev. (=Ustulina zonata (Lév.)Sacc.), occurring in the tea gardens of Assam are illustrated and described. In addition, the following species ofHypoxylon hitherto reported from this country are enlisted:H. multiforme Fr. (=H. atropurpureum Fr., =H. hookeri Berk.)H. fragiforme (Pers. ex Fr.)Kickx. (=H. coccineum Bull.),H. rubiginosum Pers. ex Fr. (=H. fusco-purpureum (Schw.)Berk. et Curt., =H. nectrioides Speg., =H. perforatum Schw. ex Fr.),H. hypomiltum Mont.,H. indicum (=H. haematostroma Mont.? =H. distillatum Berk. et Fr.),H. investiens (Schw.)Curt.,H. crocopeplum Berk. et Curt. (=H. ochraceo-flavum Berk. et Cooke),H. pistillare Pers. ex Fr.,H. haematostroma Mont. (=H. vividum Berk. et Br.).H. jecorium Berk. et Rav. (=Nummularia cinnabarina P. Henn.),H. suborbiculare (=Nummularia suborbicularis (Welw. et Currey() =H. sclerophaeum Berk. et Curt? Sacc.),H. truncatum (Schw. ex Fr.)Miller (=H. annulatum (Schw.)Mont.) andH. stygium (Lév.)Sacc.     

Deuteromycetes from jabalpur-II

Summary In this paper eighteen fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Of these one, viz.Sarcinella palawanensis (Syd.)V. P. Sahni (=Stigmella palawanensis Syd.) is described as a new combination. Among the new fungus records for India may be citedSeptoria cassiicola Kell. &Swingle onCassia fistula L.,Coniothyrium fuckelii Sacc. onAnogeissus latifolia Wall.,Phomopsis bakeri Syd. onFicus bengalensis L.,Cercospora woodfordiae Petch. onWoodfordia fruticosa (L.)Kurz.,Cercospora guanicencis Young onCaesalpinia sepiaria Roxb., andCercospora chevalieri P. Saccardo onAmorphophallus companulatus Blume.Phyllosticta buteae Syd. onButea monosperma (Lam.)Kuntze,Colletotrichum dracaenae-fragrentis (Mori)Petrak &Sydow onDracaena brachystachys Hook.,Pithomyces chartarum (Berk. &Curt.)M. B. Ellis onCassia fistula L. andCassia tora L.,Cladosporium herbarum (Pers.)Link. onButea monosperma (Lam.)Kuntze andCercospora bougainvilleae P. N. Rao onBougainvillea glabra Choisy are new state records.Discosia artocreas Tode exFr. onHolarrhena antidysentrica Wall.,Botryodiplodia theobromae Pat. onButea monosperma (Lam.)Kuntze,Colletotrichum capsici (Syd.)Butler &Bisby onButea monosperma (Lam.)Kuntze,Alternaria tenuis Nees exFr. onYucca alofolia L.,Alternaria tenuissima (Nees exFr.)Wiltshire onBougainvillea glabra Choisy, andCurvularia lunata (Wakker)Boed. onCassia tora L. are new host records.     

Peptidasen

Summary Using fresh frozen (with and without semipermeable membranes), freeze-dried or sections from aldehyde fixed material and hexazotized p-rosaniline for simultaneous coupling more than 20 different unsubstituted or substituted l-amino acid naphthylamides are split especially in the microvilli and/or stereocilia of the small intestine, kidney and epididymis from rats. Further sites of positive reactions can be revealed by l-alanyl, l-leucyl, l-lysyl, ,l-glutamyl, ,l-glutamyl, l-asparaginyl, N-benzoyl-l-arginyl, N-carbobenzoxy-l-arginyl and N-benzoyl-l-phenylalanyl 2-naphthylamide. Among the substituted and unsubstituted peptide 2-naphthylamides l-prolyl-l-arginyl 2-naphthylamide is not hydrolysed in visible amounts; l-arginyl-l-arginyl, l-alanyl-l-arginyl-l-arginyl, l-alanyl-l-leucyl-l-tyrosyl, l-histidyl-l-seryl, l-seryl-l-tyrosyl and l-glycyl-l-phenylalanyl 2-naphthylamide are metabolized in the renal and intestinal brush border; the reaction pattern obtained with N-carbobenzoxy-l-glycyl-l-glycyl-l-arginyl 2-naphthylamide differs from that of N-carbobenzoxy-l-arginyl 2-naphthylamide. In addition l-glycyl-l-prolyl, l-leucyl-l-alanyl, l-lysyl-l-alanyl and l-alanyl-l-phenylalanyl-l-prolyl 2-naphthylamide are also split in the lysosomes of many organs and the secretion granules of gland cells.

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PeptidasenI. Histochemische Untersuchungen mit 2-Naphthylamiden und Hexazonium-p-rosanilin

Zusammenfassung Mit hexazotiertem p-Rosanilin als Simultankuppler werden in frischen (mit und ohne semipermeable Membranen), gefriergetrockneten und in Schnitten von aldehyd-fixiertem Material mehr als 20 unsubstituierte oder substituierte Aminosäure-2-naphthylamide nahezu ausschließlich in den Mikrovilli und/oder Stereocilien von Dünndarm, Nebenhoden und Niere von Ratten umgesetzt. Weitere positive Reaktionsorte liefern l-Alanyl-, l-Leucyl-, l-Lysyl-, ,l-Glutamyl-, ,l-Glutamyl-, l-Asparaginyl-, N-Benzoyl-l-arginyl-, N-Carbobenzoxy-l-arginyl- und N-Benzoyl-l-phenylalanyl-2-naphthylamid. Unter den Peptid-2-naphthylamiden wird l-Prolyl-l-arginyl-2-naphthylamid nicht in sichtbarer Menge hydrolysiert; l-Alanyl-l-arginyl-l-arginyl-, l-Arginyl-l-arginyl-, l-Asparaginyl-l-arginyl-, l-Alanyl-l-leucyl-l-tyrosyl-, l-Histidyl-l-seryl-, l-Seryl-l-tyrosyl- und l-Glycyl-l-phenylalanyl-2-naphthylamid setzen der renale und intestinale Bürstensaum um. N-Carbobenzoxy-l-glycyl-l-glycyl-l-arginyl-2-naphthylamid liefert verglichen mit Benzoyl- und N-Carbobenzoxy-l-arginyl-2-naphthylamid zusätzliche Resultate. Mit l-Glycyl-l-prolyl-, l-Leucyl-l-alanyl-, l-Lysyl-l-alanyl-und l-Alanyl-l-phenylalanyl-l-prolyl-2-naphthylamid reagieren die Lysosomen zahlreicher Organe und Sekretgranula positiv.

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 105)     

Induction of aldose reductase activity inCandida guilliermondii by pentose sugars

Summary Cell extracts ofCandida guilliermondii grown ind-xylose,l-arabinose,d-galactose,d-glucose,d-mannose and glycerol as sole carbon sources possessed NADPH-dependent aldose reductase activity, but no NADH-dependent activity was detected.d-xylose andl-arabinose were the best inducers of aldose reductase activity. The highest enzyme activity ind-xylose orl-arabinose-grown cells was observed first withl-arabinose followed byd-xylose as substrates of the enzymatic reaction. However, only low activity was found ind-glucose,d-mannose andd-galactose-grown cells, indicating that these carbon sources cause catabolite repression. Enzyme activities induced ind-xylose-grown cells were twice as high as those obtained from the cells under resting conditions. Furthermore, the level of induction of aldose reductase activity depended on the initial concentration ofd-xylose. The present study shows that aldose reductase activity may be efficiently induced by pentose sugars of hemicellulosic hydrolysates and weakly by hemicellulosic hexoses.     

Racemic Resolution of some <Emphasis Type="SmallCaps">dl</Emphasis>-Amino Acids using <Emphasis Type="Italic">Aspergillus fumigatus</Emphasis><Emphasis Type="SmallCaps">l</Emphasis>-Amino Acid Oxidase

The ability of Aspergillus fumigatus l-amino acid oxidase (l-aao) to cause the resolution of racemic mixtures of dl-amino acids was investigated with dl-alanine, dl-phenylalanine, dl-tyrosine, and dl-aspartic acid. A chiral column, Crownpak CR+ was used for the analysis of the amino acids. The enzyme was able to cause the resolution of the three dl-amino acids resulting in the production of optically pure d-alanine (100% resolution), d-phenylalanine (80.2%), and d-tyrosine (84.1%), respectively. The optically pure d-amino acids have many uses and thus can be exploited industrially. This is the first report of the use of A. fumigatus l-amino acid oxidase for racemic resolution of dl-amino acids.     

Growth inhibition by L-phenylalanine in Agmenellum quadruplicatum

Summary L-Phenylalanine is a potent inhibitor of growth in a marine species of blue-green bacteria, Agmenellum quadruplicatum. The growth inhibition is reversed by many amino acids when added to the culture medium simultaneously with L-phenylalanine. The most effective L-phenylalanine antagonists are L-tyrosine, L-alanine, L-leucine, L-methionine, L-tryptophan, and L-isoleucine. However, L-tyrosine is the only effective L-phenylalanine antagonist when growth is inhibited by L-phenylalanine for two or more hours prior to addition of an equimolar concentration of the compound tested as an antagonist. Various explanations that could account for inhibition of growth by L-phenylalanine are discussed. Inhibition of growth by L-phenylalanine is not a feature peculiar to the general physiology of blue-green bacteria. For example, the growth of Anacystis nidulans, a fresh water species, was not inhibited by L-phenylalanine, although a different pattern of metabolite sensitivity was found.     

Some foliicolous ectoparasites and associated fungi from Jabalpur (M.P.) II

Summary In this paper the author has described eight more interesting foliicolous ectoparasites from Jabalpur (M.P.) India. Out of thesePyrenochaeta mitteriellae Sahni an hyperparasite onMitteriella zizyphina Syd. colonising leaves ofZizyphus xylopyra Willd.,Chaetothyrium jasminicola Sahni onJasminum sambac Ait., andMyiocopron parviflorae Sahni onIxora parviflora Vahl are reported as new species.Micropeltis ekmanii Petr. &Cif. on leaves ofCarissa spinarum L.,Asterinella ixorae Ryan on leaves ofIxora parviflora Vahl andPlenotrichum sp. on leaves ofCasearia tomentosa Roxb. are three new fungus records for this country. The remaining two fungi viz.Asterina tonduzi (Speg.)Syd. onFlacourtia ramontchi L'Herr. andBalladynopsis negrii (Cast.) M. B.Ellis onRandia dumetorum Lamk. are new state reocrds.     

Myxomycetes from Orissa (India)-III

Summary Six myxomycetous fungi,Cribraria intricata Schrad.,Elaeomyxa miyazakiensis (Emoto)Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan andPhysarella oblonga (Berk. &Curt.)Morgan have been figured and described form Cuttack, Orissa, of whichElaeomyxa miyazakiensis (Emoto) Hagelst.,Stemonitis flavogenita Jahn,Didymium vaccinum (Dur. &Mont.)Buchet,Didymium minus (Lister)Morgan are new records for India.     

Metabolism of amino acid amides in Pseudomonas putida ATCC 12633

The metabolism of the natural amino acid l-valine, the unnatural amino acids d-valine, and d-, l-phenyglycine (d-, l-PG), and the unnatural amino acid amides d-, l-phenylglycine amide (d-, l-PG-NH₂) and l-valine amide (l-Val-NH₂) was studied in Pseudomonas putida ATCC 12633. The organism possessed constitutive l-amidase activities towards l-PG-NH₂ and l-Val-NH₂, both following the same pattern of expression, suggesting the involvement of similarly regulated enzymes, or a common enzyme. Quite surprisingly, growth in mineral media with l-PG-NH₂ resulted in variable, long lag phases of growth and strongly reduced l-amidase activities. Conversion of d-PG-NH₂ into d-PG and l-PG also occurred and could be attributed to the presence of an inducible d-amidase and the racemization of the amino acid amide in combination with l-amidase activity, respectively. The further degradation of l-PG and d-PG involved constitutive l-PG aminotransferase and inducible d-PG dehydrogenase activities, respectively, both with a high degree of enantioselectivity. Amino acid racemase activity for d- and l-PG was not detected. Correspondence to: L. Dijkhuizen     

A Metal Ion as a Cofactor Attenuates Substrate Inhibition in the Enzymatic Production of a High Concentration of <Emphasis Type="SmallCaps">D</Emphasis>-glutamate Using <Emphasis Type="Italic">N</Emphasis>-acyl-<Emphasis Type="SmallCaps">D</Emphasis>-glutamate Amidohydrolase

N-Acyl-D-glutamate amidohydrolase (D-AGase) was inhibited by 94 % when 1 mol/l N-acetyl-DL- glutamate was used as a substrate. The addition of 1 mM Co²⁺ stabilized D-AGase. Moreover, the substrate inhibition was weakened to 88% with the addition of 0.4 mM Co²⁺ to the reaction mixture. Although D-AGase is a zinc-metalloenzyme, the addition of Zn²⁺ from 0.01 to 10 mM did not increase the D-glutamic acid production in the saturated substrate. Under optimal conditions, 0.38 M D-glutamic acid was obtained from N-acyl-DL-glutamate with 100% of the theoretical yield after 48 h.     

Additions to the fungi of Jabalpur (madhya pradesh) V

Summary The present paper includes ten parasitic fungi occurring at Jabalpur. These include three new species viz.Hendersonia syzygii Hasija onSyzygium cumini, Phoma ixorae Hasija onIxora sp. andPhyllosticta agarwalii Hasija onDalbergia paniculata. Colletotrichum truncatum (Schw.)Andrus &Moore onAlysicarpus bupleurifolius andPiricularia zingiberi onHedychium sp. are new fungus records for the country.Grewia hirsuta forPhyllosticta sedgwickii DaCosta &Mundkur,Lagerstroemia speciosa forPestalotiopsis versicolor (Speg.)Stey.,Zingiber officinale forCurvularia lunata (Wakker)Boedijn,Musa paradisiaca forDeightoniella torulosa (Syd.)Ellis andFlacourtia ramontchi forAlternaria tenuis Nees exPers. are new hosts record from Jabalpur, India.     

A phylogenetic analysis of the lichen familySphaerophoraceae (Caliciales); a new generic classification and notes on character evolution

A phylogenetic analysis of the familySphaerophoraceae (Caliciales, lichenized ascomycetes) has resulted in a new generic classification. Notes on character evolution are given. The generaSphaerophorus s. str.,Bunodophoron andLeifidium, gen. nov., are accepted.Pleurocybe andPseudosphaerophorus are considered synonyms ofBunodophoron andThysanophoron is considered synonym toSphaerophorus. The following new combinations are proposed:Bunodophoron coomerense (Ohlsson)Wedin,B. diplotypum (Vain.)Wedin,B. dodgei (Ohlsson)Wedin,B. flaccidum (Kantvilas & Wedin)Wedin,B. formosanum (Zahlbr.)Wedin,B. imshaugii (Ohlsson)Wedin,B. insigne (Laurer)Wedin,B. kinabaluense (M. Satô)Wedin,B. macrocarpum (Ohlsson)Wedin,B. madagascareum (Nyl.)Wedin,B. microsporum (Ohlsson)Wedin,B. murrayi (Ohlsson)Wedin,B. notatum (Tibell)Wedin,B. ohlssonii (Wedin)Wedin,B. patagonicum (C. W. Dodge)Wedin,B. ramuliferum (I. M. Lamb)Wedin,B. scrobiculatum (C. Bab.)Wedin,B. tibellii (Wedin)Wedin,B. whakapapaense (Wedin)Wedin, andLeifidium tenerum (Laurer)Wedin.     

Chromosome numbers of some species of the genus<Emphasis Type="Italic">Hieracium</Emphasis> s.str. (<Emphasis Type="Italic">Asteraceae</Emphasis>) from Turkey

Results of a karyological analysis of eight species collected from northeast Anatolia, Turkey are presented. The following chromosome numbers were determined:H. medianiforme (Litv. etZahn)Juxip 2n=36,H. karagollense (Zahn)P.D. Sell etC. West 2n=36,H. argillaceoides (Litv. etZahn)Juxip 2n=36,H. amblylepis Boiss. 2n=27,H. lanceolatum Vill. 2n=27,H. asterodermum (Woronow etZahn)Juxip 2n=27,H. umbellatum L. 2n=18,H. laevigatum Willd. 2n=27. The chromosome numbers of five of these seven species are presented for the first time. The results show a high proportion of triploids and tetraploids within the taxa studied.     

Zur taxonomischen Stellung und Nomenklatur vonMicropus longifolius (Compositae-Inuleae)

Zusammenfassung Die unter den NamenMicropus longifolius Boiss. etReut. bzw.Cymbolaena longifolia (Boiss. etReut.)Smoljan. gut bekannte Art wurde zuerst alsStylocline griffithii A.Gray beschrieben. Ihre taxonomische Stellung in bezug auf die GattungenMicropus undStylocline wird diskutiert. Es erscheint am richtigsten, die Art in eine eigene monotypische Gattung zu stellen, ihr korrekter Name ist dannCymbolaena griffithii (A.Gray)Wagenitz, comb. nov.

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Summary The species usually known asMicropus longifolius Boiss. etReut. orCymbolaena longifolia (Boiss. etReut.)Smoljan. was described for the first time in 1873 asStylocline griffithii A.Gray. The taxonomic position in relation to the generaMicropus andStylocline is discussed. It seems most appropriate to regard this species as belonging to a monotypic genus. In this case its correct name isCymbolaena griffithii (A.Gray)Wagenitz, comb. nov.

     

Deuteromycetes from Jabalpur. III

Summary In this paper the author has described seventeen new Deuteromycetes from Jabalpur (M.P.) India. These are: Phyllosticta careyae Sahni onCareya arborea Roxb.,Phyllosticta anogeissi Sahni onAnogeissus latifolia Wall.,Phomopsis dalbergiae Sahni onDalbergia sissoo Roxb.,Phomopsis yuccae Sahni onYucca aloifolia L.,Phomopsis dracaenae Sahni onDracaena brachystachys Hook.,Phomopsis buteae Sahni onButea monosperma (Lam.)Kunze,Cytospora grevilleae Sahni onGrevillea robusta A. Cunn.,Coniothyrium sarcinellae Sahni onSarcinella palawanensis (Syd.)Sahni parasitising leaves ofCalastrus paniculatus Willd.,Coniothyrium dioscoreae Sahni on fruits ofDioscorea sp.,Amerodiscosiella indica Sahni onIxora parviflora Vahl.,Ascochyta nyctanthis Sahni onNyctanthes arbor-tristis L.,Pseudodiplodia buteae Sahni onButea monosperma (Lam.)Kunze,Pseudodiplodia oreodoxae Sahni onOreodoxa oleracea Mart.,Phaeoseptoria bougainvilleae Sahni onBougainvillea glabra Choisy,Hainesia jabalpurensis Sahni onWoodfordia fruticosa (L.)Kurz.,Colletotrichum arjunae Sahni onTerminalia arjuna W. & A., andColletotrichum terminaliae Sahni onTerminalia bellirica (Gaertn.)Roxb.     

On the molecular structure of chromosomes

Summary It is suggested that the construction of molecular models of the chromosome may prove to be a fruitful way of attacking the fundamental problems of cytology and genetics. Certain micelles consisting of molecules of protamine-nucleate are suggested for consideration. They are designed to interpret in molecular terms the postulates of genetics and the nature of the gene, and are constructed with special reference to the findings of the cytologists as to the capacity of the chromosomes to swell, to contract, to grow and to divide.Based upon a lecture given in the physics department of the University of Manchester on May 13, 1935. The Author is indebted to W. T.Astbuby, L. E.Bayliss, J. D.Bernal, D.Crowfoot, C.D.Darlington, P.& G.Eggleton, J.M.Gulland, L.J.Harris, D.Jordan Lloyd, M. F.Howson, W. O.Kermack, E. H.Neville, M.Polanyi and J. Z.Young for advice and criticism.     

Production ofd-mannitol fromd-aldopentoses by the yeastRhodotorula minuta

Rhodotorula minuta produced 16% ofd-mannitol and 3% ofd-arabinitol when cultivated ond-ribose, 4% of mannitol and 11% of arabinitol when grown ond-xylose, 5% ofd-mannitol and 5% ofd-arabinitol when grown ond-arabinose, and 5% ofd-mannitol and 6% ofd-arabinitol when cultivated ond-lyxose.     

Deuteromycetes from Jabalpur-I

Summary In the present paper twelve fungi belonging to the form class Deuteromycetes are described from Jabalpur (M.P.) India. Among these the new host records areMonochaetia carissae Munjal &Kapoor onCarissa spinarum L.,Pestalotia neglecta Thuem. onAtylosia scarabaeoides,Benth.,Pestalotia japonica Syd. onCelastrus paniculatus Willd.,Pestalotia theae Sawada var.minor Stey. onButea monosperma (Lam.)Kuntze,Pestalotia versicolor Speg. onBuchanania lanzan Spreng.,Colletotrichum dematium (Pers. exFr.)Grove onAmorphophallus sp.,Colletotrichum lindemuthianum (Sacc. &Mag.)Bri. &Cav. onCassia tora L.,Coniella diplodiella (Speg.)Petrak &Syd. on.Anogeissus latifolia Wall.,Hendersonula toruloidea Nattrass onPhilodendron bipinnatifidum Schott. andMyrothecium roridum Tode exFr. onCasearia tomentosa roxb. The above fungi include two species (Pestalotia neglecta Thuem. andPestalotia theae Sawada var.minor Stey.) which have been described for the first time from this country.Sphaeropsis tumefasciens Hedges onCitrus medica L. var.acida L. is a new record from this state; andExcipularia narsapurensis Subramanian, also a new record from the state, is reported for the first time on a named host.     

Bemerkungen zu einigenStipagrostis-Arten (Gramineae) aus Afrika und Arabien

Summary A new species from Egypt (Sinai) and Arabia is described asStipagrostis multinerva H.Scholz. Stipagrostis arabiaefelicis Bor 1967 is a synonym ofSt. drarii (V.Täckholm)de Winter 1963 (Aristida drarii V.Täckholm 1941). The taxonomic status ofStipagrostis foexiana (Maire etWilczek)de Winter (Aristida obtusa subsp.pubescens Andreánszky) in North and South Africa is discussed. Furthermore some new data on taxonomy and distribution of the following species are recorded:Stipagrostis acutiflora subsp.algeriensis (Henrard) comb, nov. et stat. nov.,St. ciliata (Desf.)de Winter,St. lanata (Forsk.)de Winter,St. libyca (H.Scholz) comb, nov.,St. papposa (Trin. etRupr.)de Winter,St. raddiana (Savi)de Winter,St. shawii (H.Soholz) comb, nov., andSt. sokotrana (Vierhapper)de Winter.      

Removal of <Emphasis Type="SmallCaps">l</Emphasis>-alanine from the production of <Emphasis Type="SmallCaps">l</Emphasis>-2-aminobutyric acid by introduction of alanine racemase and <Emphasis Type="SmallCaps">d</Emphasis>-amino acid oxidase

l-2-Aminobutyric acid can be synthesized in a transamination reaction from l-threonine and l-aspartic acid as substrates by the action of threonine deaminase and aromatic aminotransferase, but the by-product l-alanine was produced simultaneously. A small amount of l-alanine increased the complexity of the l-2-aminobutyric acid recovery process because of their extreme similarity in physical and chemical properties. Acetolactate synthase has been introduced to remove the pyruvate intermediate for reducing the l-alanine concentration partially. To eliminate the remnant l-alanine, alanine racemase of Bacillus subtilis in combination with d-amino acid oxidase of Rhodotorula gracilis or Trigonopsis variabilis respectively was introduced into the reaction system for the l-2-aminobutyric acid synthesis. l-Alanine could be completely removed by the action of alanine racemase of B. subtilis and d-amino acid oxidase of R. gracilis; thereby, high-purity l-2-aminobutyric acid was achieved. The results revealed that alanine racemase could discriminate effectively between l-alanine and l-2-aminobutyric acid, and selectively catalyzed l-alanine to d-alanine reversibly. d-Amino acid oxidase then catalyzed d-alanine to pyruvate stereoselectively. Furthermore, this method was also successfully used to remove the by-product l-alanine in the production of other neutral amino acids such as l-tertiary leucine and l-valine, suggesting that multienzymatic whole-cell catalysis can be employed to provide high purity products.