首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 42 毫秒
1.
变灰青霉固态发酵降解植酸的初步研究   总被引:7,自引:0,他引:7  
从发霉植酸钠溶液中分离到一株产植酸酶的变灰青霉(Penicilliumcanescens)P4。以麸皮:玉米面:黄豆饼粉=7:2:1为主要培养基成份,用优选法确定最适培养基为在上述基本培养基中添加4%(NH4)2SO4,1%葡萄糖,1.5倍水,自然pH。发酵过程的动态分析表明,该菌在上述条件下28℃恒温培养6d后植酸降解率可达90%;无机磷含量由0.13%增至0.57%;可溶性蛋白含量由3.80%增至7.60%。用4%CaCl·2HO水溶液抽提  相似文献   

2.
斯达油脂酵母U9018产胞外多糖的适宜条件   总被引:2,自引:0,他引:2  
研究了斯达油脂酵母U9018产胞外多糖的适宜条件:培养基组成(g/L):蔗糖100,蛋白胨7,KHPO435,KHPO·3H.5,MgSO·7HO0.2,MnSO·HO0.01,FeSO·7HO0.01,NaCl0.01,初始PH6.5。装液量  相似文献   

3.
江均平   《微生物学通报》1996,23(5):316-317
将1mm厚凝固于复印膜上的水琼脂(15%~2.0%)凝胶板侵入含12mmol/L植酸钠的Gly—HCl缓冲液中达1h以上,取出干至胶表面无水迹,于上加Aspergillussp.59—2植酸酶或与电泳后的凝胶板紧贴10~60min。然后水平置于恒温水浴锅中反应一定时间,取出浸入1mol/LHSO-2%(NHMn24-10%FeSO4相似文献   

4.
少动鞘脂单胞菌S1胞外多糖发酵工艺条件研究   总被引:9,自引:0,他引:9  
研究了摇瓶培养条件下少动鞘脂单胞菌引的胞外多糖的发酵工艺。S1菌的发酵产胶可采用二步发酵法:第一阶段,培养基成分为:蔗糖10g,NHNO 0.5g,KHOP 0.5g,MnSO 0.3g,MgSO 0.1g,吐温80 0.02g溶于蒸馏水并定容至1000mL,pH7.2±0.1,温度33℃~35℃,高溶氧。第二阶段,发酵 10~12h后,补加蔗糖40g/L,温度  相似文献   

5.
尖镰孢胞外青霉素V酰化酶的产生   总被引:1,自引:0,他引:1  
冯瑛  崔福绵   《微生物学通报》1996,23(6):329-332
由腐殖土中分离出一株产胞外青霉素V酰化酶的尖镰孢(Fusariumoxysporum),编号FP941。研究了该菌在液体培养基中产胞外青霉素V酰化酶的条件。在以10%麦麸为碳源的培养基中,添加氮源能促进酶的形成。无机氮源优于有机氮源。(NHHPO的促进效果最佳,草酸铵次之,用量均为1%。为提高产酶量,培养基中添加诱导物是必要的、苯氧乙酸的诱导效果最佳,用量为0.1%,其次是青霉素V,用量为0.3%。最适培养条件为:培养  相似文献   

6.
从土壤中分离到一株黄杆菌(Flavobacteriumspp)CF-60,该菌的生长需Mg2+存在,MgSO·7HO的最适浓度为0.2%;蛋白胨是该菌株生长的最好氮源,它不能利用无机氮。种龄超过96h的菌体不能在新鲜培养基中生长。经54h的2L恒化器发酵,生物量达6.8g/L,色素产量为10.6mg/L。该菌产生的类胡萝卜素成分简单,主要成分的含量为90.3%,该成分经初步鉴定是分子结构中含有羰基和羟  相似文献   

7.
数理统计方法优化单细胞蛋白发酵培养基研究   总被引:3,自引:1,他引:2  
采用正交试验和中心组合设计相结合的数理统计方法,在2L发酵罐中对紫云英汁液培养单细胞蛋白发酵培养基进行筛选、优化,试验结果表明较优培养基为:初糖2.3%,酵母粉0.16%,KHPO0.2%,MgSO0.05%。酵母浓度最高达10.46g/L,基质生长得率为0.55g菌体/g基质,基质转化率为83%。研究结果同时说明采用数理统计方法设计实验,工作量小,效率高,结果准确。  相似文献   

8.
芽孢杆菌M_(50)产生β甘露聚糖酶的条件研究   总被引:3,自引:0,他引:3  
从土壤中分离到9 株产生β甘露聚糖酶的芽孢杆菌( Bacillus sp .) 。Bacillussp . M50250m L三角瓶摇瓶培养试验,以4 % 的魔芋粉为碳源,1-0 % ( NH4)2SO4 为氮源,0-35 %Na2CO3 ,30 ~34 ℃培养60h 产酶达到高峰。酶活力为180 ~200u/m L。100L 罐发酵,在30 ~32 ℃,1∶0 .75vvm 通气量,200r/min 条件下,发酵液酶活力高达330u/m L。酶的最适反应温度和pH 分别为50 ℃和6-0 ,低于50 ℃,pH5 .0 ~7 .0 酶稳定。Fe3+ 、Al3+ 、EDTA、Hg2+ 对酶有抑制作用,而Ba2+ 、Mn2+ 对酶有激活作用。发酵粗酶液对苎麻精干麻精练,显示对精干麻的半纤维素残胶具有降解作用。  相似文献   

9.
从农药厂废水中分离到6株能以除草剂阿特拉津为唯一氮源生长的细菌,即假单胞菌(Pseu-domonas spp.)AD1、AD2和 AD6,土壤杆菌(Agrobacterium sp.)AD4,黄单胞菌(Xanthomonas sp.)ADS,欧文氏菌(Erwinia sp.)AD7。AD1菌株能使无机盐培养基中的 0.3g/L阿特拉津在72h内降解99,9%。当以AD1、AD2、AD4、AD5、AD6和AD7菌株的总DNA为模板进行PCR扩增时,除A  相似文献   

10.
纤维素酶酶解稻壳的条件试验   总被引:22,自引:0,他引:22  
本文报道康氏木霉N-78(Trichodermakoningi)纤维素酶产生和酶解稻壳的适宜条件。实验结果表明,在稻草粉麸皮固体培养基上,纤维素酶产生的适宜条件为稻草粉和麸皮的比例为7:3,培养基含水量为250%,pH为6.0—6.5,温度为30℃,时间为3d。酶解稻壳的最适条件为:pH为4.4,温度为40℃,作用时间为3d,酶曲量和底物量比例为1:3。  相似文献   

11.
12.
13.
14.
15.
Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

16.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

17.
18.
Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.  相似文献   

19.
正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases  相似文献   

20.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号