甘松油生产技术

一、甘松与甘松油简介甘松属败酱科甘松香属多年生草本植物。分为中华甘松(Nardostachys Chinensis Batalin)和长匙甘松(Nardostachys jtamansi),是一种珍贵的经济植物,其主要分布我国四川、云南、甘肃、青海、西藏等省(区),以及印度、尼泊尔等国家。生长于阴湿山坡及湿     

假百合核型的研究

假百合,Notholirion bulbiliferum (Lingelsh.) Stearn [N.hyacinthinum (Wils.) Stapf]是百合科假百合属,Notholirion Wall.ex Boiss.的多年生草本植物,产于我国陕西、甘肃、四川、西藏和云南等省区;尼泊尔、锡金、不丹和印度也有分布。假百合陕西民间叫“太白米”,是一种药用植物。 假百合属共4种,做过染色体计数者3种。本文首次报道了假百合的染色体数目,并做了核型分析,旨在与百合属Lilium L.的核型作一比较,为探讨这两属间的亲缘关系提供必要的细胞学资料。     

甘肃中南部原尾虫物种多样性及区系分析

在甘肃中南部设置12个样区,对该地区原尾虫的物种多样性进行了研究。发现原尾虫11种,隶属于2目6科7属。其中包括1个甘肃新记录属、4个甘肃新记录种和1个未定名种。古蚖科Eosentomidae、檗蚖科Berberentulidae为优势类群。华山蚖Huashanentulus huashanensis为广布种;檗蚖科Berberentulidae有2属3种;古蚖科Eosentomidae有3属7种,占甘肃原尾虫总数的44％;始蚖科Protentomidae有1属1种;夕蚖科Hesperentomidae有1属2种;日本蚖科Nipponentomidae有1种,中华雅娃蚖Yavanna sinensis是甘肃的新记录种;蚖科Hesperentomidae有1属2种,包括1个华山蚖属未定名种Huashanentulus sp.,只分布在临夏太子山自然保护区。对甘肃原尾虫区系进行了分析,结果显示：古北界种类占总数的20％,东洋界物种占53.3％,广布种类占总数的26.7％。     

甘肃棘豆内生细菌的分析与研究

甘肃棘豆属于疯草,有毒,为豆科棘豆属多年生草本植物,严重影响我国西南牧区的牲畜安全。植物内生细菌是指定殖在植物组织内部但不引起明显病害症状的细菌,它具有许多生物学功能作用。本文以甘肃棘豆种子为研究对象对其内生细菌进行了分离,并利用形态观察、生化试验等方法进行了初步鉴定。实验共分离到3株菌株,2株杆菌,1株球菌。所得菌株为后续研究甘肃棘豆内生细菌与甘肃棘豆所含毒素的相互关系提供了细菌菌株来源。     

封面说明

布袋兰[Calypsobul bosa（L．）Oakes],隶属于兰科,兰亚科,树兰族,布袋兰属,该属仅1～2种。为地生兰,常生于云杉林或其他针叶林下,在四川、甘肃,其生长地海拔可达3200m。为多年生草本植物,地下具假鳞茎,偶见珊瑚状根状茎。     

甘肃忍冬科植物区系地理

对甘肃产忍冬科植物8属59种8变种及3亚种（不包括引种栽培的1属和3种）*进行了统计,并计算甘肃与邻近省的相似性系数,与陕西省的最大,为0．41;而与西藏的为最小,仅为0．14。陇南西部河谷亚热带湿润区和陇南北部暖温带区是甘肃产忍冬科植物种类最为丰富的地区。同时,对甘肃产忍冬科植物进行了属、种分布区类型的划分,属的分布区类型可划分为：旧世界温带分布、温带亚洲分布、东亚分布和中国特有4个类型。种的分布类型可划分为：中国特有、东亚分布、温带亚洲分布和旧世界温带分布。从该科植物在甘肃的分布来看,绝大多数种类都不超过中国4个植物亚区在甘肃的交汇点,与中国植物区划基本相符,并可确切说此交汇点应在兰州的永登县附近。     

甘肃悬钩子属系统分类及区系地理研究

甘肃省产悬钩子属植物３４种、８变种、隶属于３组１１亚组,其中５个种为甘肃分布新记录、个新种及１个新变种。本文根据实地调查和标本鉴定,编制了系统检索表,并对甘肃悬钩子属植物区系成分及区系成进行了初步探讨。     

甘肃两栖动物的保护研究

调查表明甘肃有两栖类动物24种,其中有尾目3科3属3种,无尾目6科21种.属于古北界的有5种和亚种,属东洋界的有14种,其余5种为两界兼有种.对引起甘肃省两栖类动物种群衰退、变异和灭绝的主要因素进行了初步分析,提出了若干针对甘肃两栖类动物的保护对策.     

东辽河河岸带草本植物物种多样性及群落数量分析

采用样线法调查了东辽河河岸带草本植物群落,分析了物种多样性特征,利用组平均聚类法和除趋势对应分析(DCA)分别对植物群落进行了分类和排序,探讨了影响东辽河河岸带草本植物群落分布的主要环境因子。结果表明:东辽河河岸带共有草本植物118种,属36科70属,草本植物的物种多样性自上游向下游呈现先增加后降低的变化趋势,而丰富度指数变化规律不显著,这与河流水文特征、河岸土壤基质类型、土壤含水量和人为干扰有关。聚类分析结果显示,东辽河河岸带草本群落可划分为11种类型,其中湿生植物群落占据优势。DCA排序轴与环境因子的相关性分析表明,海拔和河流等级是东辽河河岸带草本植物群落分布的重要影响因子。     

甘肃裸子植物区系地理分析

在对甘肃产裸子植物进行系统整理的基础上,探讨了甘肃裸子植物的区系特征和区系分区。甘肃裸子植物种类丰富,产５科１５属４５种。其特点是：松柏类发达;北温带分布型属和中国特有种占优势;富含第三纪残遗成分;区系乱分具明显交汇过渡性质。依据甘肃各区域生态地理因子分异特点和裸子植物地理分布规律,将甘肃裸子植物区系划分为５个自然的区系小区：走廊区、中部小区、甘南小区、陇南小区。     

A Unique Protease Cleavage Site Predicted in the Spike Protein of the Novel Pneumonia Coronavirus (2019-nCoV) Potentially Related to Viral Transmissibility

正Dear Editor,In December 2019, a novel human coronavirus caused an epidemic of severe pneumonia(Coronavirus Disease 2019,COVID-19) in Wuhan, Hubei, China(Wu et al. 2020; Zhu et al. 2020). So far, this virus has spread to all areas of China and even to other countries. The epidemic has caused 67,102 confirmed infections with 1526 fatal cases     

Curcuminoids as inhibitors of thioredoxin reductase: A receptor based pharmacophore study with distance mapping of the active site

Curcumin is the yellow pigment of turmeric that interacts irreversibly forming an adduct with thioredoxin reductase (TrxR), an enzyme responsible for redox control of cell and defence against oxidative stress. Docking at both the active sites of TrxR was performed to compare the potency of three naturally occurring curcuminoids, namely curcumin, demethoxy curcumin and bis-demethoxy curcumin. Results show that active sites of TrxR occur at the junction of E and F chains. Volume and area of both cavities is predicted. It has been concluded by distance mapping of the most active conformations that Se atom of catalytic residue SeCYS498, is at a distance of 3.56 from C13 of demethoxy curcumin at the E chain active site, whereas C13 carbon atom forms adduct with Se atom of SeCys 498. We report that at least one methoxy group in curcuminoids is necessary for interation with catalytic residues of thioredoxin. Pharmacophore of both active sites of the TrxR receptor for curcumin and demethoxy curcumin molecules has been drawn and proposed for design and synthesis of most probable potent antiproliferative synthetic drugs.     

Comparative morphology of the carpel in the Liliaceae: Hewardieae, Petrosavieae, and Tricyrteae

The young pistils in the melanthioid tribes, Hewardieae, Petrosavieae and Tricyrteae, are uniformly tricarpellate and syncarpous. They lack raphide idioblasts. All are multiovulate, with bitegmic ovules. The Petrosavieae are marked by the presence of septal glands and incomplete syncarpy. Tepals and stamens adhere to the ovary in the Hewardieae and the Petrosavieae but not in the Tricyrteae. Two vascular bundles occur in the stamens of the Hewartlieae and Tricyrtis latifolia. Ventral bundles in the upper part of the ovary of the Hewardieae are continuous with compound septal bundles and placental bundles in the lower part. Putative ventral bundles occur in the alternate position in the Tricyrteae and putative placental bundles in the opposite. position in the Petrosavieae. The dichtomously branched stigma in each carpel of the Tricyrteae is supplied by a bifurcated dorsal bundle.     

Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection

Highlights
1. The N-terminal tail of histone H3 is specifically cleaved during EV71 infection.
2. Viral protease 3C is identified as a protease responsible for proteolytically processing the N-terminal H3 tail.
3. Our finding reveals a new epigenetic regulatory mechanism for Enterovirus 71 in virus-host interactions.     

Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen’s Encephalitis

Rasmussen’s encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and 16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis, intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8⁺ T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8⁺ T cells are involved in the pathogenesis of RE.     

Perinatal Vertical Transmission of Chikungunya Virus in Ruili,a Town on the Border between China and Myanmar

正Dear Editor,Chikungunya virus (CHIKV), an arbovirus in the family of Togaviridae, genus Alphavirus, is transmitted by the A.aegyptii or A. albopictus mosquito, and causes disease in humans characterized by fever, rash, and arthralgia (Silva and Dermody 2017; Suhrbier 2019). It was first reported in 1953 in Tanzania, and caused only a few outbreaks and sporadic cases in Africa and Asia in last century. However, in the epidemic in 2004, CHIKV acquired mutations that conferred enhanced transmission by the A. albopictus mosquito(Schuffenecker et al. 2006). Since then, it has successively caused outbreaks in Africa, the Indian Ocean, South East Asia, the South America, and Europe (Zeller et al. 2016).     

Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2

In conclusion, the novel visual RT-LAMP assay is a simple, rapid, and sensitive approach for detection of SARS-CoV-2, and it is ready for application in primary care and community hospitals or health care centers, and even patients' own houses in response to the current SARS-CoV-2 epidemic because the assay does not require sophisticated equipment and skilled personnel. Furthermore, it is also ready to be used in fields for screening samples from wild animals and environments to facilitate the identification of potential intermediate hosts that mediate the cross-species transmission of SARS-CoV-2 from bats to humans.