两种过路黄的核型研究

对国产报春花科（Ｐｒｉｍｕｌａｃｅａｅ）珍珠菜属（Ｌｙｓｉｍａｃｈｉａ）植物过路黄（Ｌ.ｃｈｒｉｓｔｉｎａｅＨａｎｃｅ）和疏节过路黄（Ｌ.ｒｅｍｏｔａＰｅｔｉｔｍ）的核型进行了首次报道。过路黄染色体数目为２ｎ＝２４,核型公式为２ｎ＝２４＝２ｍ＋４ｓｍ＋６ｓｔ＋１２ｔ,核型类型“３Ａ”;疏节过路黄染色体数目为２ｎ＝２２,核型公式为２ｎ＝２２＝４ｍ＋６ｓｍ＋２ｓｔ＋１０ｔ,核型类型“３Ａ”。     

山东10种植物的核型分析

对山东１０ 种植物进行了核型分析。茴茴蒜（ Ｒａｎｕｎｃｕｌｕｓｃｈｉｎｅｎｓｉｓ Ｂｇｅ－） 染色体数目２ｎ ＝１６ , 核型公式Ｋ（２ｎ） ＝ ２ｘ ＝ １６ ＝ ２ Ｍ ＋ ２ｍ ＋ ２ｓｍ ＋ １０ｓｔ, “３Ａ”类型; 五脉地椒（ Ｔｈｙｍｕｓｑｕｉｎｑｕｅｃｏｓｔａｔｕｓ Ｃｅｌａｋ－） 染色体数目２ｎ＝ ２６ , 核型公式Ｋ （２ｎ） ＝ ２ｘ＝ ２６ ＝ ８ Ｍ ＋ １８ｍ , “１Ａ”类型; 蛇床（ Ｃｎｉｄｉｕｍ ｍｏｎｎｉｅｒｉ（Ｌ－） Ｃｕｓｓ－） 染色体数目２ｎ＝ ２０ , 核型公式Ｋ （２ｎ） ＝ ２ｘ＝ ２０ ＝ ２Ｍ＋ １６ｍ ＋ ２ｓｍ , “２Ｂ”类型; 波斯菊（ Ｃｏｓｍｏｓ ｂｉｐｉｎｎａｔｕｓ Ｃａｖ－） 染色体数目２ｎ ＝ ２４ , 核型公式Ｋ（２ｎ） ＝ ２ｘ ＝ ２４ ＝ １６ｍ ＋ ２ｍ （ｓａｔ） ＋ ６ｓｍ , “２Ａ”类型; 白车轴草（ Ｔｒｉｆｏｌｉｕｍ ｒｅｐｅｎｓ Ｌ－） 染色体数目２ｎ＝ ３２ , 核型公式Ｋ （２ｎ） ＝ ４ｘ ＝ ３２ ＝ ３２ｍ , “１Ａ”类型; 铁苋菜（ Ａｃａｌｙｐｈａ ａｕｓｔｒａｌｉｓ Ｌ－）染色体数目２ｎ ＝ ３２ , 核型公式Ｋ （２ｎ） ＝ ２ｘ＝ ３２ ＝ ３２ｍ , “１Ｂ”类型; 地构叶（ Ｓｐｅｒａｎｓｋｉａ ｔ?     

湖南6种毛莨科植物的核型研究

本文对产于湖南的６种毛莨科植物的染色体进行了研究。裂叶星果草［Ａｓｔｅｒｏｐｙｒｕｍｃａｖａｌｅｒｉｅｉ（Ｌｅｖｌ．ｅｔＶａｎｔ）Ｄｒｕｍｍ．ｅｔＨｕｔｃｈ．）的染色体属于Ｒ型,核型公式为２ｎ＝１６＝１２ｍ＋２ｓｍ＋２ｔ,核型类型属于ＺＢ;打破碗花花（ＡｎｅｍｏｎｅｈｕｐｅｈｎｓｉｓＬｅｍ．）的核型公式为２ｎ＝１６＝１０ｍ＋４ｓｔ＋２ｔ（２ｓａｔ）,核型类型属于２Ａ;粗齿铁线莲［Ｃｌｅｍａｔｉｓａｐｉｉｆｏｌｉａｖａｒ．ａｒｇｅｎｔｉｌｕｃｉｄａ（Ｌｅｖｌ．ｅｔＶａｎｔ）Ｗ．Ｔ．Ｗａｎｇ］的核型公式为２ｎ＝１６＝１０ｍ＋２ｓｔ＋４ｔ（２ｓａｔ）或２ｎ＝１６＝１０ｍ＋２ｓｔ＋４ｔ（４ｓａｔ）随体染色体数目在居群之间有变化,核型类型属于２Ａ;扬子铁线莲［Ｃｌｅｍａｔｉｓｇａｎｉｐｉｎｉａｎａ（Ｌｅｖｌ．ｅｔＶａｎｔ）Ｔａｍｕｒａ］的核型公式为２ｎ＝１６＝１０ｍ＋２ｓｔ＋４ｔ（４ｓａｔ）,核型类型属于２Ｂ;毛莨（ＲａｎｕｎｃｕｌｕｓｃａｎｔｏｎｉｅｎｓｉｓＤＣ．）的核型公式为２ｎ＝１６＝６ｍ＋４ｓｔ＋６ｓｔ（２ｓａｔ）,核型类型属于３Ａ;毛莨（ＲａｎｕｎｃｕｌｕｓｊａｐｏｎｉｃｕｓＴｈｕｎｂ．）的核型公式为２ｎ＝?     

浙江鸭跖草属植物的核型研究

本文报道了浙江二种鸭跖草属植物──饭包草ＣｏｍｍｅｌｉｎａｂｅｎｇａｌｅｎｓｉｓＬ．和鸭跖草ＣｏｍｍｅｌｉｎａｃｏｍｍｕｎｉｓＬ．的染色体数目与核型：ＣｏｍｍｅｌｉｎａｂｅｎｇａｌｅｎｓｉｓＬ．核型为Ｋ（２ｎ）＝２２＝１８ｍ＋４ｓｍＣｏｍｍｅｌｉｎａｃｏｍｍｕｎｉｓＬ．核型为Ｋ（２ｎ）＝２２＝４Ｍ＋１２ｍ＋４ｓｍ＋２ｓｔ     

七种药用植物的染色体研究

对山东７种药用植物的染色体进行了研究。结果表明：田旋花（ＣｏｎｖｏｌｖｕｌｕｓａｒｖｅｎｓｉｓＬ）的染色体数目为２ｎ＝７８;蜜柑草（ＰｈｙｌａｎｔｈｕｓｍａｔｓｕｍｕｒａｅＨａｖａｔａ）的染色体数目为ｎ＝８８;挂红灯（ＰｈｙｓａｌｉｓａｌｋｅｋｅｎｇｉＬｖａｒｆｒａｎｃｈｅｔｉ（Ｍａｓｔ）Ｍａｋｉｎｏ）的染色体数目为２ｎ＝２４,核型公式为Ｋ（２ｎ）＝２４＝２ｍ＋１８ｓｍ＋２ｓｔ＋２ｓｔ（ｓａｔ）,核型“２Ａ”型;无剌曼陀罗（ＤａｔｕｒａｓｔｒａｍｏｎｉｕｍＬｖａｒｉｎｅｒｍｉｓ（Ｊａｃｑ）ＳｃｈｉｎｚｅｔＴｈｅｌ）的染色体数目为２ｎ＝２４,核型公式为Ｋ（２ｎ）＝２４＝２０ｍ＋４ｓｍ,核型“１Ｂ”型;决明（ＣａｓｉａｔｏｒａＬ）的染色体数目为２ｎ＝２６,核型公式为Ｋ（２ｎ）＝２６＝２４ｍ＋２ｓｍ,核型“１Ａ”型;荔枝草（ＳａｌｖｉａｐｌｅｂｅｉａＲＢｒ）的染色体数目为２ｎ＝１６,核型公式为Ｋ（２ｎ）＝１６＝６ｍ＋１０ｓｍ,核型“２Ａ”型;车前（ＰｌａｎｔａｇｏａｓｉａｔｉｃａＬ）的染色体数目为２ｎ＝３６,核型公式为Ｋ（２ｎ）＝３６＝３２ｍ＋４ｓｍ,核型“１Ａ”型。     

阴山荠属和泡果荠属植物染色体数目及过氧化物酶同工酶的比较

阴山荠属和泡果荠属受试种类的染色体数目是：柔毛阴山荠（Ｙ．ｈｅｎｒｙｉ（Ｏｌｉｖ．）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,叉毛阴山荠（Ｙ．ｆｕｒｃａｔｏｐｉｌｏｓａ（Ｋｕａｎ）Ｙ．Ｈ．Ｚｈａｎｇ）２ｎ＝１２,双牌泡果荠（Ｈ．ｓｈｕａｎｇｐａｉｅｎｓｉｓＺ．Ｙ．Ｌｉ）２ｎ＝４４,黟县泡果齐（Ｈ．ｙｉｃｉａｎｅｎｓｉｓＹ．Ｈ．Ｚｈａｎｇ）２ｎ＝４２,双牌泡果（Ｈ．ｐａｒｄｏｘａ（Ｈａｎｃｅ）Ｙ．Ｈ．Ｚｈａｎ     

安徽石蒜属4种植物核型研究

本文分析了安徽境内石蒜属ＬｙｃｏｒｉｓＨｅｒｂ．４种植物的核型,并结合有关文献探讨它们的核型变异。结果表明,乳白石蒜Ｌ．ａｌｂｉｆｏｌｉａＫｏｉｄｚ．的核型为２ｎ＝１９＝３ｍ＋７ｓｔ＋４ｔ＋５Ｔ,属３Ｂ型;中国石蒜Ｌ．ｃｈｉｎｅｎｓｉｓＴｒａｕｂ．为２ｎ＝１６＝６ｍ＋１０Ｔ,属３Ｂ型;石蒜Ｌ．ｒａｄｉａｔａ（Ｌ’Ｈｅｒ．）Ｈｅｒｂ．为２ｎ＝２２＝４ｓｔ＋１８ｔ,属４Ａ型;换锦花Ｌ．ｓｐｒｅｎｇｅｒｉＣｏｍｅｓ．ｅｘＢａｋｅｒ．为２ｎ＝２２＝２ｓｔ＋２０ｔ,属４Ａ型。以上各种植物均未发现随体和次缢痕,通过核型比较,可见同种不同居群间在染色体数和核型结构上均存在较大的变异。     

宝铎草和长穗开口箭的核型

本文对湖南产的宝锋草（ＤｉｓｐｏｒｕｍｓｅｓｓｉｌｅＤ．Ｄｏｎ）和广西产的长穗开口箭（ＴｕｐｉｓｔｒａｌｏｎｇｉｓｐｉｃｇＹ．ＷａｎｅｔＸ．Ｈ．Ｌｕ）的核型作了分析。前者的核型公式为２ｎ＝１４＝４ｇ＋１０ｓｍ（２ｓａｔ）,核型类型属于３Ａ;后者的核型公式为２ｎ＝３８＝２４ｍ＋６ｓｉｎ＋８ｓｔ．核型具有明显的二型性,属于２Ｃ型。此外还讨论了宝锋草的核型变异以及开口箭属和蜘蛛抱蛋属（Ａｓｐｉｄｉｓｔｒａ）的关系。     

山东四种草本植物的核型研究

本文对山东４种草本植物进行了染色体研究。结果表明：阿尔泰狗哇花（Ｈｅｔｅｒｏｐａｐｐｕｓａｌ ｔａｉｃｕｓ（Ｗｉｌｄ）Ｎａｖｏｐｏｋｒ）的染色体数目为２ｎ＝３６,核型公式为Ｋ（２ｎ）＝３６＝３６ｍ,核型“１Ａ”型;求米草（Ｏｐｌｉｓｍｅｎｕｓｕｎｄｕｌａｔｉｆｏｌｉｕｓ（Ａｒｄｕｉｎｏ）ＲｏｅｍｅｔＳｃｈｕｌｔ）的染色体数目为２ｎ＝１２,核型公式为Ｋ（２ｎ）＝１２＝８ｍ＋４ｓｍ,核型“２Ａ”型;红秋葵（Ｈｉｂｉｓｃｕｓｃｏｃｉｎｅｕｓ（Ｍｅｄｉｃ）Ｗａｌｔ）的染色体数目为２ｎ＝３８,核型公式为Ｋ（２ｎ）＝３８＝１４ｍ＋２２ｓｍ＋２ｓｔ,核型“２Ｂ”型;蟋蟀草（Ｅｌｅｕｓｉｎｅｉｎｄｉｃａ（Ｌ）Ｇａｅｒｔｎ）的染色体数目为２ｎ＝１８,核型公式为ｋ（２ｎ）＝１８＝１６ｍ＋２ｓｍ,核型“２Ａ”型。     

国产稗属植物的细胞学研究

对国产７种（含变种）稗属Ｅｃｈｉｎｏｃｈｌｏａ植物的细胞学进行了初步研究。９个供试材料的体细胞分别表现两种染色体数目２ｎ为３６或５４。除光头稗Ｅ．ｃｏｌｏｎｕｍＬ外,分析了其余种的核型,其结果为：稗Ｅ．ｃｒｕｓ．ｖａｒ．ｃｒｕｓｇａｌｌｉ２ｎ＝５４＝５８ｍ＋６ｓｍ;西来稗Ｅ．ｃｒｕｓ．ｖａｒ．ｚｅｌａｙｅｎｓｉｓ（Ｈ．Ｂ．Ｋ．）Ｈｉｔｃｈｃ ２ｎ＝５４＝４２ｍ（２ＳＡＴ）＋１２ｓｍ（４ＳＡＴ）;     

东北小鲵中枢神经系统形态学与组织学初步研究

本文应用脊椎动物神经标本制作法和HE染色法,对东北小鲵中枢神经系统的外部形态和组织学结构进行了初步研究,描述了东北小鲵神经系统形态和组织学结构的特点,并与无尾两栖类和爬行类相对比,探讨了有尾两栖类的进化地位。结果表明:与无尾两栖类(如蛙)相比,东北小鲵中枢神经系统中,大脑半球较小,结构较为原始,小脑结构简单,是两栖类中较为原始的类群。此外,东北小鲵开始具有了臂神经丛和骶神经丛,但没有爬行类的发达,可作为两栖类向爬行类进化的证据之一。     

Genetic relationships among salamanders of the genus Hynobius (Amphibia, Caudata) from Korea and southwestern Japan

We performed allozyme analysis for three Korean (Hynobius leechii, H. quelpaertensis, and H. yangi) and three Japanese (H. nebulosus, H. tsuensis, and H. dunni) salamanders to clarify their interspecific relationships using H. naevius as an outgroup. The genetic distances (Nei's D) within ingroup species ranged from 0.11 to 0.78 with a mean of 0.33. In the NJ and CONTML trees, monophyly of the ingroup was not supported and Korean H. quelpaertensis and H. leechii diverged first from the remaining species, which together formed a weakly supported clade. Korean H. yangi, long identified as H. leechii, was closer to Japanese H. nebulosus (D=0.108) and H. tsuensis (D=0.138) than to Korean H. leechii (D=0.197) and H. quelpaertensis (D=0.305). Hynobius tsuensis and H. nebulosus were very close (D=0.108) despite their different breeding habits. A geohistorical hypothesis is proposed to explain the divergence of the six species.     

A new species of lentic breeding Korean salamander of the genus Hynobius (Amphibia, Urodela)

A new species of salamander, Hynobius yangi, is described from Kori (=Hyoam-ri), Busan-shi, southeastern Korea. It is a lentic breeder belonging to the H. nebulosus species group, and has long been confused with another Korean species H. leechii. The new species, however, is genetically substantially differentiated from Korean and Japanese relatives. Hynobius yangi is morphologically very similar to H. leechii, but could be differentiated from it by the tail shape, degree of limb separation, shape of vomerine teeth series, shape of egg sac, and dorsal coloration.     

Karyotypic analysis of two algae species Scenedesmus incrassatulus Bohl and Scenedesmus antennatus Bréb (Chlorophyta, Chlorococcales)

The karyotypes (number, morphology and size of the chromosomes) of two algae species of Scenedesmus genus, S. incrassatulus and S. antennatus, were studied. The karyotype of S. incrassatulus (n=4) was asymmetric, characterized by two large metacentric, one large submetacentric and one small metacentric chromosomes. The karyotype assembly of S. antennatus (n=6) reveals two metacentrics and four submetacentrics. This karyotype was symmetric. The general chromosomal formulae of both species, as well as the total average metaphase length of their haploid set are presented. The results of chromosomal studies of other related species are compared and discussed. Data from the karyotypic analysis showed that S. incrassatulus, S. antennatus and S. obliquus are separate biological species from taxonomical point of view.     

东北小鲵中枢神经系统形态学与组织学初步研究（图版Ⅵ，上）

本文应用脊椎动物神经标本制作法和HE染色法,对东北小鲵中枢神经系统的外部形态和组织学结构进行了初步研究,描述了东北小鲵神经系统形态和组织学结构的特点,并与无尾两栖类和爬行类相对比,探讨了有尾两栖类的进化地位。结果表明：与无尾两栖类（如蛙）相比,东北小鲵中枢神经系统中,大脑半球较小,结构较为原始,小脑结构简单,是两栖类中较为原始的类群。此外,东北小鲵开始具有了臂神经丛和骶神经丛,但没有爬行类的发达,可作为两柄类向爬行类进化的证据之一。     

Karyotype of a Japanese salamander Hynobius katoi and its implication on breeding ecology (Amphibia: Caudata)

Karyotype of a Japanese small salamander, Hynobius katoi, was first described. All individuals examined had 2n=58 chromosomes, consisting of nine pairs of biarmed macrochromosomes, four pairs of biarmed medium-sized chromosomes, six pairs of biarmed microchromosomes, and 10 pairs of uniarmed microchromosomes, although distinction of the second and the third groups of chromosome pairs was not clear. All pairs appeared homologous and no sexual dimorphism was found. Possession of 2n=58 chromosomes in H. katoi strongly suggests its lotic-breeding habits as was expected from the number and size of eggs and adult morphology. When compared morphology of chromosomes among lotic-breeders with 2n=58 chromosomes, metacentric nature of No. 10 seems to characterize the karyotype of H. katoi.     

Mitochondrial DNA data unveil highly divergent populations within the genus <Emphasis Type="Italic">Hynobius</Emphasis> (Caudata: Hynobiidae) in South Korea

Korean salamanders of the genus Hynobius are currently classified into 3 species, H. leechii, H. quelpaertensis, and H. yangi. To investigate the phylogenetic relationship of these species, we analyzed the partial sequence of mitochondrial cytochrome b gene (907 bp) of 197 specimens from 43 regions in South Korea. Of these specimens, 93 were additionally examined with 12S rRNA (799 bp). Based on the partial sequence of the mitochondrial cytochrome b gene and 12S rRNA, 89 and 36 haplotypes were defined, respectively, consisting of six subclades (H. leechii, H. quelpaertensis, H. yangi, HC1, HC2, and HC3). Among these subclades, the three subclades (HC1, HC2, and HC3) were clearly separated from the 3 previously reported species in the genus Hynobius. Pairwise sequence divergence between the six subclades ranged from 6.3 to 11.2% in cytochrome b gene and 2.0 to 4.3% in 12S rRNA. These results indicate there may be more divergent populations than the three currently described. Moreover, the estimation of divergence time revealed that the Hynobius species in South Korea diverged during the Miocene epoch, approximately 9 — 5 MYA. In addition, we confirmed the distribution of the three known species (H. leechii, H. quelpaertensis, and H. yangi) and determined the distributions of new, distinct groups (or subclades; HC1, HC1, and HC3). To more accurately establish the taxonomic status and population structure, further genetic, morphological, and ecological studies will be needed.     

挂榜山小鲵的核型(有尾目:小鲵科)

本文首次报道了挂榜山小鲵Hynobius guabangshanensis 的核型.其2n=56,分为4组,其中9对大型染色体,4对双臂中型染色体,5对双臂小型染色体和10对单臂小型染色体.挂榜山小鲵具有池塘类型小鲵的特征,即2n=56以及缺少中端着丝粒染色体,这与挂榜山小鲵的生态和形态特征也属池塘类型相符.     

Genome sizes and chromosomes in the basal metazoan Hydra

Hydras belong to one of the earliest eumetazoan animal groups, but to date very little is known about their genome sizes, gene numbers, and chromosomes. Here we provide genome size estimates and corresponding karyotypes for five Hydra species. Nuclear DNA contents were assessed by slide-based Feulgen microphotometry. Hydra oligactis possesses the largest genome of 1450 Mbp, followed by similar 1 C capacities in H. carnea (1350 Mbp), H. vulgaris (1250 Mpb) and H. circumcincta (1150 Mbp). The smallest genome of 380 Mbp was determined in H. viridissima. While the number of chromosomes is identical in all five Hydra species (2n = 30), the size of the chromosomes is strictly correlated to the size of the genome, with H. viridissima having conspicuously small chromosomes. The taxonomic and evolutionary significance of the C-value and chromosomal size variation in this ancient group of metazoans as well as its impact on genomic organization and forthcoming genome projects are discussed.     

Systematic Relationships of Hynobius okiensis among Japanese Salamanders (Amphibia: Caudata)

We conducted an electrophoretic survey to examine systematic relationships of a lotic-breeding salamander Hynobius okiensis endemic to Dogo Island of the Oki Islands, Japan, with several lentic and lotic-breeding Japanese species. Genetically H. okiensis with 2n=56 chromosomes was closer to the lentic-breeding H. nebulosus group (H. nebulosus and H. dunni) with the same chromosome number than to the lotic-breeding H. naevius group (H. naevius and H. kimurae) and H. boulengeri with 58 chromosomes. Chromosome number reduction from 58 to 56, possibly accompanied with a change in breeding environment from streams to still waters, is estimated to have first occurred in the nebulosus group of Hynobius. A reversal only in breeding habits then seems to have followed in steep, montane environments of the small island of Dogo, resulting in the speciation of H. okiensis.