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1.
青霉菌木聚糖酶粗酶制剂制备和性质   总被引:3,自引:1,他引:2  
本文研究了青霉菌P1固态发酵培养的产酶过程,发酵4天产木聚糖酶活力最高,固体曲最适浸提比为1:7(w/v),通过65%饱和度的硫酸铵盐析,获得的木聚糖酶效果较好。40℃烘干酶粉活力为1305.6IU/g,得率为69.5%。酶反应最知适pH4.6,最适温度50℃。酶与底物半纤维素作用的亲合性大小顺序为:麸皮〉稻草〉蔗粉〉玉米蕊。  相似文献   

2.
木霉T6木聚糖酶制剂研究   总被引:2,自引:0,他引:2  
本文研究了木霉T6(Trichoderma sp.)产木聚糖酶固态发酵过程和木聚糖酶制剂制备,结果显示,固体曲培养4d时酶活力最高,固体曲最适液固浸提比为7:1。木聚糖酶在60~65%硫酸铵饱和度下盐析效果最好。冷冻干燥和40℃烘干酶粉得率分别为68.3%和45.7%。酶最适反应PH为4.5,最适反应温度50℃,在不同温度下1小时后的半失活温度为47.7℃。  相似文献   

3.
海洋真菌Asteromyces sp.TM76木聚糖酶生产初步研究   总被引:1,自引:0,他引:1  
研究了海洋真菌Asteromyces sp.TM76的生长动力学和发酵产酶情况。在生长培养基中,TM76菌的最大比生长速率为1.875(1/d),倍增时间为8.87h;TM76发酵过程中所产木聚糖酶有两个生产高峰,第7d,有最大木聚糖酶活力470.5U/mL。发酵起始pH为6.5时对产酶最佳。酶学性质研究表明该菌所产木聚糖酶的最适反应pH和温度分别为6.5和55℃,在不同温度保温1h,测定酶的半失活温度为63℃。  相似文献   

4.
酸性木聚糖酶产生菌的筛选及产酶条件   总被引:21,自引:0,他引:21  
从150株真菌中筛选到8株产木聚糖酶活力在100U/mL以上的菌株,其中活力最高的为黑曲霉(编号149)(Aspergilusniger)。该菌株产酶较适培养基为:麸皮半纤维素4%,NaNO31%,麸皮1%,用不加(NH4)2SO4和尿素的Mandels氏营养盐液配制。28℃~30℃振荡培养60h,酶活力最高可达375.2U/mL。该酶最适作用pH为46,在pH3~11之间基本稳定。该菌株发酵液中含有木聚糖酶(相对活力100)外还有淀粉酶(18),甘露聚糖酶(098),β木糖苷酶(094)和纤维素酶(017)。  相似文献   

5.
曲霉木聚糖酶发酵条件与性质   总被引:14,自引:0,他引:14  
从实验室培养污染物上分离出一株产木聚糖酶活力高的曲霉(AspergillusSp.)A3菌株。研究了其发酵过程,该菌经30℃,培养96小时,酶活力可达320IU/ml,研究了碳源,氮源,发酵起始pH值及通风量对产酶的影响。酶的最适反应温度为55℃,最适pH值为4.4。在不同温度下保温1小时,测得该酶的半失活温度为51℃。  相似文献   

6.
果胶酶CP-85211菌株的选育及其液体发酵条件的研究   总被引:6,自引:1,他引:5       下载免费PDF全文
黑曲霉(Aspergillus niger) CP-831经0.03%亚硝基呱在40℃处理30分钟,获得一株高产果胶酶突变株CP-85211。该突变株发酵滤液,当以果胶为底物时,酶活力为308u/m’;当以多聚半乳糖醛酸为底物时,酶活力为842u/ml。产酶活力水平约为出发菌种的5倍。产酶最适培养基组成为:8%麦麸,2%桔皮粉和2%硫酸铵。最适培养条件为:起始pH 3.5,如℃,72小时。  相似文献   

7.
木聚糖酶生产菌株的筛选及产酶条件的优化   总被引:6,自引:0,他引:6  
以甘蔗渣半纤维素为碳源,从垃圾场土壤中分离到6株分解半纤维素的菌株。通过固态发酵的木聚糖酶活力比较筛选到1株木聚糖酶活力较高的菌株。该菌株18S rDNA序列与曲霉(Aspergillus sp.)的同源性达97%,根据对菌株形态学分析和18S rDNA序列分析的结果,将该菌株鉴定为曲霉HQ3。HQ3的最佳产酶条件为:甘蔗渣:麸皮为7:3(W/W),固液比为1:4(W/W),尿素0.4 %,pH7.0,温度30℃,发酵产酶时间4 d。在最佳产酶条件下,其木聚糖酶活最高可达3421U/g干曲。  相似文献   

8.
一株产木聚糖酶的黑曲霉固态发酵产酶性质的研究   总被引:3,自引:0,他引:3  
目的:选育产木聚糖酶活力高的黑曲霉菌株,对其产酶条件进行优化,并研究其酶学性质。方法:通过木聚糖酶解木聚糖产生透明圈的方法,筛选产木聚糖酶菌株,测定固体发酵培养基中玉米芯与麸皮的比例、培养温度、培养时间、添加氮源对产酶的影响。进行了作用温度、pH值、金属离子对酶活力的影响试验,以及酶不同温度下的热稳定性的试验。结果:从自然界筛选得到一株产木聚糖酶的黑曲霉菌株,通过对固态发酵培养条件优化,最终产酶水平达到了5500u/g固体干曲。酶的最适作用温度是45℃、最适作用pH值4.8,是一种偏酸性酶。该酶在45℃以上的温度保存会使酶活力迅速丧失,Mg^2+、Zn^2+对该酶有激活作用,而Mn^2+、Cu^2+、Hg^2+则完全抑制酶的活性。结论:选育的黑曲霉菌株产木聚糖酶活力较高,培养条件简单。  相似文献   

9.
木霉菌株T6木聚糖酶固态发酵条件和酶学性质研究   总被引:11,自引:0,他引:11  
吴克  蔡敬民等 《菌物系统》2001,20(2):191-195
研究了碳源和氮源、起始pH、接种量及温度等条件对一野生型木霉Trichodermasp.T6菌株固态发酵产木聚糖酶的影响。在28℃培养4d后,酶活力可达1918IU/g干培养物。酶的最适反应温度为50℃,最适反应pH4.5。不同温度保温1h后,测定酶的半失活温度为47.7℃,酶的pH稳定性也进行了研究。  相似文献   

10.
角质蛋白酶固态发酵工艺及酶解条件的研究   总被引:2,自引:0,他引:2  
张道海  李楠 《生物技术》1994,4(4):11-14
曲霉A28-8是一株优良的角蛋白酶分泌突变菌株。其最佳固体发酵培养基为:20%羽毛粉,80%麸皮和微量无机氮(每克培养基中加0.5mg);最适产酶条件为:起始pH7.5~8.0,温度28℃~30℃,时间为60~70小时,酶活高达2500KU/g曲;最适酶解条件为pH7.0~9.0,温度45℃~50℃。  相似文献   

11.
产木聚糖酶厌氧真菌菌株筛选及产酶培养条件研究*   总被引:4,自引:0,他引:4  
从12株分离自反刍动物瘤胃及粪样的厌氧真菌中筛选到一株木聚糖酶高产菌,编号为A4,初步鉴定为Neocallimastix属菌。以稻草秸、玉米秸、花生秸、滤纸片段为发酵底物,经39℃厌氧培养,A4菌产生的木聚糖酶活分别为14.31U/mL、11.39U/mL、6.99U/mL和13.38U/mL。对A4菌产生木聚糖酶的条件进行优化,结果发现,培养基中无细胞瘤胃液浓度对A4菌产生的木聚糖酶活无显著影响;但酵母膏浓度从1.0g/L降至0.5g/L后,A4菌产生的木聚糖酶活显著下降(P<0.05)。  相似文献   

12.
一株产木聚糖酶链霉菌的鉴定及发酵产酶*   总被引:8,自引:0,他引:8  
以木聚糖为唯一碳源,筛选出一株高产木聚糖酶生产菌株。该菌株经形态特征、 培养特征、生理生化和细胞壁组分分析等实验,鉴定为卷须链霉菌(Streptomyces cirratus).摇瓶发酵产酶实验表明:培养基最佳初始pH值为6.0;玉米芯水不溶木聚糖和蛋白胨分别是最佳的碳源和氮源;添加0.5%吐温80使得木聚糖酶活力提高到原来的2.5倍,发酵液最高酶活达到623u/mL。  相似文献   

13.
Optimization of enzyme production from Aspergillus niger ATCC 6275 under both submerged and solid-substrate cultivation was investigated. Results from submerged cultivation using palm oil mill effluent revealed that pretreatment of ground palm cake did not improve enzyme production. Addition of 0.60g NH4NO3/l generated maximum activity of xylanase and cellulase (CMCase). The optimum aeration rate was 1.2 v/v min. Under solid-substrate cultivation, the results indicated that heating and alkali treatment of the ground palm cake gave no further improvement in enzyme production. The optimal N-source was 2% urea. Optimal initial moisture contents for xylanase and CMCase activities were 60% and 50% respectively, with temperature optima of 30°C and 35°C, respectively. The optimal inoculum size was 1× 108 spores/g palm cake with an initial pH of 4.5–5.0. The maximum activities of xylanase (282.9U/g) and CMCase (23.8U/g) were obtained under the optimum conditions. Solid-substrate cultivation was a better method for the production of enzyme, particularly xylanase, from A. niger ATCC 6275. The application of these enzymes to decanter effluent showed the separation of oil and grease and suspended solids from the effluent. This is comparable to the result achieved from using the commercial xylase preparation Meicelase and superior to the effect of Sumyzyme.  相似文献   

14.
Thermomyces lanuginosus, isolated from self-heated jute stacks in Bangladesh, was studied for production of high level of cellulase-free thermostable xylanase at 50°C using xylan. Optimization of the medium composition was carried out on shake-flask level using Graeco-Latin square technique. This increased xylanase production from 527 nkat ml−1 in the original medium to 9168–9502 nkat ml−1 in the optimized medium under optimized culture conditions e.g. initial medium pH (6.0–6.5), culture temperature (50°C) and time (5–6 d). The lag phase was very much shorter in the laboratory reactor compared to which existed in the shake cultures and 7111 nkat of xylanase activity were obtained per ml of culture filtrate at 60 h of cultivation. With a 15 min reaction time, the optimal pH and temperature for the xylanase activity were at 6.5 and 65°C, respectively. The enzyme was almost stable over a broad range of pH 3–9 at 20°C, with an optimum stability at pH 6.5. After 51 h heating at 50°C the enzyme retained 60%, 100% and 90% activity at pH 5.0, 6.5 and 8.0, respectively. The crude enzyme could hydrolyse xylan effectively and in only 6 h 67.3%, 54.0% and 49.2% saccharifications were achieved for 2%, 5% and 10% substrate levels, respectively. The principal product of hydrolysis was xylobiose together with smaller amounts of xylooligosaccharides (degree of polymerization 3–7) and xylose.  相似文献   

15.
Enzyme production by a new mesophilic Streptomyces isolate was investigated which grew optimally on 1% (w/v) xylan and 10% (w/v) wheat bran at pH 7 and 37 °C. Xylan induced only CMCase (0.29 U/ml) besides xylanase (22–35 U/ml, 40–49 U/mg protein). Wheat bran induced xylanase (105 U/ml, 17.5 U/mg protein), CMCase (0.74 U/ml), -xylosidase (0.009 U/ml), -glucosidase (0.026 U/ml), -L-arabinofuranosidase (0.049 U/ml), amylase (1.6 U/ml) and phytase (0.432 U/ml). The isolate was amenable to solid state cultivation and produced increased levels of xylanase (146 U/ml, 28 U/mg protein). The pH and temperature optima of the crude xylanase activity were 5.5 and 65 °C respectively. The pI was 6.0 as determined by PEG precipitation. The crude enzyme was applied in treatment of paper pulp and predigestion of poultry feed and was found to be effective in releasing sugars from both and soluble phosphorus from the latter.  相似文献   

16.
A very high level of alkalophilic and thermostable pectinase and xylanase has been produced from newly isolated strains of Bacillus subtilis and Bacillus pumilus respectively. Enzyme production for pectinase was carried out under SSF using combinations of cheap agricultural residues while xylanase was produced under submerged fermentation using wheat bran as substrate to minimize the cost of production of these enzymes Among the various substrates tested, the highest yield of pectinase production was observed by using combination of WB + CW (6592 U/g of dry substrate) supplemented with 4% yeast extract when incubated at 37 °C for 72 h using deionized water of pH 7.0 as moistening agent. The biobleaching effect of these cellulase free enzymes on kraft pulp was determined. Both xylanase and pectinase showed stability over a broad range of pH from 6 to 10 and temperature from 55 to 70 °C. The bleaching efficiency of the pectinase and xylanase on kraft pulp was maximum after 150 min at 60 °C using enzyme dosage of 5 IU/ml of each enzyme at 10% pulp consistency with about 16% reduction in kappa number and 84% reduction in permanganate number. Enzyme treated pulp when subjected to CDED1D2 steps, 25% reduction in chlorine consumption and upto 19% reduction in consumption of chlorine dioxide was observed for obtaining the same %ISO brightness. Also an increase of 22 and 84% in whiteness and fluorescence respectively and a decrease of approximately 19% in the yellowness of the biotreated pulp were observed by pretreatment of the pulp with our enzymatic mixture.  相似文献   

17.
Purification and characterization of xylanase from Aspergillus ficuum AF-98   总被引:1,自引:0,他引:1  
Lu F  Lu M  Lu Z  Bie X  Zhao H  Wang Y 《Bioresource technology》2008,99(13):5938-5941
The purification and characterization of xylanase from Aspergillus ficuum AF-98 were investigated in this work. The extracellular xylanase from this fungal was purified 32.6-fold to homogeneity throughout the precipitation with 50–80% (NH4)2SO4, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 chromatography. The purified xylanase (specific activity at 288.7 U/ mg protein) was a monomeric protein with a molecular mass of 35.0 kDa as determined by SDS-PAGE. The optimal temperature and pH for the action of the enzyme were at 45 °C and 5.0, respectively. The xylanase was activated by Cu2+ up to 115.8% of activity, and was strongly inhibited by Hg2+, Pb2+ up to 52.8% and 89%, respectively. The xylanase exhibited Km and Vmax values of 3.267 mg/mL, 18.38 M/min/mg for beechwood xylan and 3.747 mg/mL, 11.1 M/min/mg for birchwood xylan, respectively.  相似文献   

18.
[目的] 研究樟绒枝霉(Malbranchea cinnamomea) CAU521利用农业废弃物固体发酵产木聚糖酶的发酵条件.[方法]采用单因素试验法优化影响菌株产酶的各个条件,包括碳源种类、氮源种类、初始pH、初始水分含量、培养温度及发酵时间共6个因素.[结果]获得的最佳产酶条件为:稻草为发酵碳源、2%(W/W)的酵母提取物为氮源、初始pH 7.0、初始水分含量80%和发酵温度45℃.在此条件下发酵6d后木聚糖酶的酶活力达到13 120 U/g干基碳源.[结论]樟绒枝霉固体发酵产木聚糖酶的产酶水平高,生产成本低,具有潜在的工业化应用前景.  相似文献   

19.
黑曲霉木聚糖酶的底物特异性和低聚木糖生产   总被引:9,自引:1,他引:8  
对黑曲霉(Aspergillus niger)木聚糖酶进行了纯化研究,结果表明,经Sephadex G-100和DEAE-SephadexA-50分离后,获得三个组分,称为X1、X2、X3。它们经PAGE电泳分析均为单一组分。对X1、X2、X3的相关性质,特别是底物特异性也作了研究,纯酶X3组分或部分纯化的酶可用于生产低聚木糖,产品得率为10%。  相似文献   

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