河南荥阳官庄遗址两周时期人牙结石的植物淀粉粒

本文对河南官庄遗址两周时期人牙结石开展了淀粉粒分析,发现了数量丰富的淀粉粒以及少量植硅体。可鉴定形态的淀粉粒主要来自粟、黍、小麦、小豆等农作物以及坚果、块茎植物;植硅体中则有来自水稻颖片的双峰型植硅体。此次研究表明官庄先民食物来源广泛,包括多种农作物和采集植物,其中粟黍类农作物在食物构成中占据主要地位,而小麦所占比例很可能已仅次于粟黍。结合包括官庄遗址和周边若干遗址的浮选结果,两周时期中原腹心地区仍是北方传统的粟作农业,但农作物种植已明显多样化;与此同时,小麦在农作物体系中的重要性增加,中原地区农作物种植体系由以粟黍为主转向以小麦为主的趋势已经出现。     

Early Eurasian migration traces in the Tarim Basin revealed by mtDNA polymorphisms

The mitochondrial DNA (mtDNA) polymorphisms of 58 samples from the Daheyan village located in the central Taklamakan Desert of the Tarim Basin were determined in this study. Among the 58 samples, 29 haplotypes belonging to 18 different haplogroups were analyzed. Almost all the mtDNAs belong to a subset of either the defined Western or Eastern Eurasian pool. Extensive Eastern Eurasian lineages exist in the Daheyan population in which Northern‐prevalent haplogroups present higher frequencies. In the limited existing Western Eurasian lineages, two sub‐haplogroups, U3 and X2, that are rare in Central Asia were found in this study, which may be indicative of the remnants of an early immigrant population from the Near East and Caucasus regions preserved only in the Tarim Basin. The presence of U3 in modern and archeological samples in the Tarim Basin suggests that the immigration took place earlier than 2,000 years ago and points to human continuity in this area, with at least one Western lineage originating from the Near East and Caucasus regions. Am J Phys Anthropol 142:558–564, 2010. © 2010 Wiley‐Liss, Inc.     

Genetics and geography of wild cereal domestication in the near east

About 12,000 years ago, humans began the transition from hunter-gathering to a sedentary, agriculture-based society. From its origins in the Near East, farming expanded throughout Europe, Asia and Africa, together with various domesticated plants and animals. Where, how and why agriculture originated is still debated. But newer findings, on the basis of genome-wide measures of genetic similarity, have traced the origins of some domesticated cereals to wild populations of naturally occurring grasses that persist in the Near East. A better understanding of the genetic differences between wild grasses and domesticated crops adds important facets to the continuing debate on the origin of Western agriculture and the societies to which it gave rise.     

Les premières étapes de la colonisation de l’Europe et l’arrivée de l’Homme sur les rives de la Méditerranée

Over recent years, many discoveries have renewed our knowledge about the oldest stone industries and also about the behaviour and lifestyle of the hominids that made them, not only in East Africa, but also in the Near East, in Trans Caucasia and in southern Europe. If the first tools making hominids appear in East Africa as early as 2.55 million years ago, they are present in the Levant a little over 2 million years ago, as early as 1.81 million years ago at the gates of Europe in Trans-Caucasia, and a little over 1.4 million years ago on the Mediterranean coasts of Europe.     

Mitochondrial sequence analysis of Salamandra taxa suggests old splits of major lineages and postglacial recolonizations of central Europe from distinct source populations of Salamandra salamandra

Representatives of the genus Salamandra occur in Europe, Northern Africa and the Near East. Many local variants are known but species and subspecies status of these is still a matter of dispute. We have analysed samples from locations covering the whole expansion range of Salamandra by sequence analysis of mitochondrial D-loop regions. In addition, we have calibrated the rate of divergence of the D-loop on the basis of geologically dated splits of the closely related genus Euproctus. Phylogenetic analysis of the sequences suggests that six major monophyletic groups exist (S. salamandra, S. algira, S. infraimmaculata, S. corsica, S. atra and S. lanzai) which have split between 5 and 13 million years ago (Ma). We find that each of the Salamandra species occupies a distinct geographical area, with the exception of S. salamandra. This species occurs all over Europe from Spain to Greece, suggesting that it was the only species that has recolonized Central Europe after the last glaciation. The occurrence of specific east and west European haplotypes, as well as allozyme alleles in the S. salamandra populations suggests that this recolonization has started from at least two source populations, possibly originating in the Iberian peninsula and the Balkans. Two subpopulations of S. salamandra were found that are genetically very distinct from the other populations. One lives in northern Spain (S. s. bernardezi) and one in southern Italy (S. s. gigliolii). Surprisingly, the mitochondrial lineages of these subpopulations group closer together than the remainder S. salamandra lineages. We suggest that these populations are remnants of a large homogeneous population that had colonized Central Europe in a previous interglacial period, approximately 500 000 years ago. Animals from these populations were apparently not successful in later recolonizations. Still, they have maintained their separate genetic identity in their areas, although they are not separated by geographical barriers from very closely related neighbouring populations.     

Genetic diversity of donkey populations from the putative centers of domestication

Donkey domestication drastically changed ancient transport systems in Africa and Asia, enabling overland circulation of people and goods and influencing the organization of early cities and pastoral societies. Genetic studies based on mtDNA have pointed to the African wild ass as the most probable ancestor of the domestic donkey, but questions regarding its center of origin remain unanswered. Endeavoring to pinpoint the geographical origin of domestic donkey, we assessed levels and patterns of genetic diversity at 15 microsatellite loci from eight populations, representing its three hypothesized centers of origin: northeast Africa, the Near East and the Arabian Peninsula. Additionally, we compared the donkey genotypes with those from their wild relative, the African wild ass (Equus africanus somaliensis) to visualize patterns of differentiation among wild and domestic individuals. Obtained results revealed limited variation in levels of unbiased expected heterozygosity across populations in studied geographic regions (ranging from 0.637 in northeast Africa to 0.679 in the Near East). Both allelic richness (Ar) and private allelic richness presented considerably higher values in northeast Africa and in the Arabian Peninsula. By looking at variation at the country level, for each region, we were able to identify Sudan and Yemen as the countries possessing higher allelic richness and, cumulatively, Yemen also presented higher values for private allelic richness. Our results support previously proposed northeast Africa as a putative center of origin, but the high levels of unique diversity in Yemen opens the possibility of considering this region as yet another center of origin for this species.     

Genomic ancestry of North Africans supports back-to-Africa migrations

North African populations are distinct from sub-Saharan Africans based on cultural, linguistic, and phenotypic attributes; however, the time and the extent of genetic divergence between populations north and south of the Sahara remain poorly understood. Here, we interrogate the multilayered history of North Africa by characterizing the effect of hypothesized migrations from the Near East, Europe, and sub-Saharan Africa on current genetic diversity. We present dense, genome-wide SNP genotyping array data (730,000 sites) from seven North African populations, spanning from Egypt to Morocco, and one Spanish population. We identify a gradient of likely autochthonous Maghrebi ancestry that increases from east to west across northern Africa; this ancestry is likely derived from "back-to-Africa" gene flow more than 12,000 years ago (ya), prior to the Holocene. The indigenous North African ancestry is more frequent in populations with historical Berber ethnicity. In most North African populations we also see substantial shared ancestry with the Near East, and to a lesser extent sub-Saharan Africa and Europe. To estimate the time of migration from sub-Saharan populations into North Africa, we implement a maximum likelihood dating method based on the distribution of migrant tracts. In order to first identify migrant tracts, we assign local ancestry to haplotypes using a novel, principal component-based analysis of three ancestral populations. We estimate that a migration of western African origin into Morocco began about 40 generations ago (approximately 1,200 ya); a migration of individuals with Nilotic ancestry into Egypt occurred about 25 generations ago (approximately 750 ya). Our genomic data reveal an extraordinarily complex history of migrations, involving at least five ancestral populations, into North Africa.     

Out of southern East Asia: the natural history of domestic dogs across the world

The origin and evolution of the domestic dog remains a controversial question for the scientific community, with basic aspects such as the place and date of origin, and the number of times dogs were domesticated, open to dispute. Using whole genome sequences from a total of 58 canids (12 gray wolves, 27 primitive dogs from Asia and Africa, and a collection of 19 diverse breeds from across the world), we find that dogs from southern East Asia have significantly higher genetic diversity compared to other populations, and are the most basal group relating to gray wolves, indicating an ancient origin of domestic dogs in southern East Asia 33 000 years ago. Around 15 000 years ago, a subset of ancestral dogs started migrating to the Middle East, Africa and Europe, arriving in Europe at about 10 000 years ago. One of the out of Asia lineages also migrated back to the east, creating a series of admixed populations with the endemic Asian lineages in northern China before migrating to the New World. For the first time, our study unravels an extraordinary journey that the domestic dog has traveled on earth.     

Ancient DNA reveals the maternal genetic history of East Asian domestic pigs

Zoo-archaeological and genetic evidence suggest that pigs were domesticated independently in Central China and Eastern Anatolia along with the development of agricultural communities and civilizations. However, the genetic history of domestic pigs, especially in China, has not been fully explored. In this study, we generate 42 complete mitochondrial DNA sequences from ～7500- to 2750-year-old individuals from the Yellow River basin. Our results show that the maternal genetic continuity of East Asian domestic pigs dates back to at least the Early to Middle Neolithic. In contrast, the Near Eastern ancestry in European domestic pigs saw a near-complete genomic replacement by the European wild boar. The majority of East Asian domestic pigs share close haplotypes, and the most recent common ancestor of most branches dates back to less than 20,000 years before present, inferred using new substitution rates of whole mitogenomes or combined protein-coding regions. Two major population expansion events of East Asian domestic pigs coincided with changes in climate, widespread adoption of introduced crops, and the development of agrarian societies. These findings add to our understanding of the maternal genetic composition and help to complete the picture of domestic pig evolutionary history in East Asia.     

Mitochondrial DNA reveals multiple introductions of domestic chicken in East Africa

Chicken were possibly domesticated in South and Southeast Asia. They occur ubiquitously in East Africa where they show extensive phenotypic diversity. They appeared in the region relatively late, with the first undisputed evidence of domestic chicken in Sudan, around ~ 700 BC. We reveal through a detailed analysis of mitochondrial DNA D-loop sequence diversity of 512 domestic village chickens, from four East African countries (Kenya, Ethiopia, Sudan, Uganda), the presence of at least five distinct mitochondrial DNA haplogroups. Phylogeographic analyses and inclusion of reference sequences from Asia allow us to address the origin, ways of introduction and dispersion of each haplogroup. The results indicate a likely Indian subcontinent origin for the commonest haplogroup (D) and a maritime introduction for the next commonest one (A) from Southeast and/or East Asia. Recent introgression of commercial haplotypes into the gene pool of village chickens might explain the rare presence of two haplogroups (B and C) while the origin of the last haplogroup (E) remains unclear being currently observed only outside the African continent in the inland Yunnan Province of China. Our findings not only support ancient historical maritime and terrestrial contacts between Asia and East Africa, but also indicate the presence of large maternal genetic diversity in the region which could potentially support genetic improvement programmes.     

Distinguishing wild and domestic wheat and barley spikelets from early Holocene sites in the Near East

Identifying morphologically domestic cereals is essential to understanding the origins of agriculture. Charred spikelet bases provide the best evidence for distinguishing wild from domestic varieties of emmer, einkorn and barley; however until now, identification criteria have not been agreed upon or well established. We examined more than 20,000 remains of charred spikelets from eleven early Holocene sites in the Near East, classing them into nine groups. We show that damage and fragmentation of wheat spikelets probably due to dehusking makes identification problematic, and only when the abscission scar is well preserved is it possible to distinguish wild spikelets which shatter from domestic spikelets which adhere and separate during threshing. Barley spikelets were found to be less damaged and more easily identified, perhaps because the processing was less damaging. Einkorn was dominant over emmer on early sites, whereas on later sites emmer was dominant. Identifications presented here from eleven sites date from approximately 13000 to 8200 cal b.p. They give an incomplete picture, but no domestic cereals were identified during the PPNA (Pre-pottery Neolithic A). Early PPNB Aswad produced domestic barley but at other sites for this period the evidence is inconclusive. Unequivocal signs of domestic emmer spikelets appear during the Middle PPNB about 10,000 years ago but wild forms continue as part of the crop after this period. These conclusions are based on limited data. Future studies will undoubtedly produce a more accurate picture.     

Griddles, Ovens, and Agricultural Origins: An Ethnoarchaeological Study of Bread Baking in Highland Ethiopia

An ethnoarchaeological study of highland Ethiopian griddle technology is compared to bread-baking technologies in Africa and the Near East. There is a functional relationship between the use of ovens and griddles and the presence or absence of gluten in bread ingredients. Ovens are most appropriate for cereals containing gluten and may be implicated in the selection of higher quality gluten in domesticated wheats. We conclude, based on evidence for griddle use and the performance characteristics of African cereals, that indigenous species were exploited in highland Ethiopia before Near Eastern cereals were introduced. Griddle-cooking practices that bias the preservation of Near Eastern cereals over African ones may explain the absence of African cereals in the early archaeobotanical record. [Keywords: Ethiopia, ethnoarchaeology, archaeobotany, ovens, griddles]     

Paléolithique supérieur et Mésolithique en Méditerranée : cadre culturel

The origin of the Upper Palaeolithic around the Mediterranean was the result of the local evolution, particularly in the Near East and in the Lower Nile basin, and of the migration from this zone to South-Eastern and Central Europe. The Initial Upper Palaeolithic in the Near East belt was the effect of local evolution from the industries based on Levallois concept to the industries which developed leptolithic blade technologies. This evolution is well registered in multi-layer sites in the Syro-Palestinian belt (Emirian/Ahmarian), which was the starting point of the diffusion of these “transitional” industries in South-Eastern and Central Europe. This diffusion could be identified with the migration of first anatomically Modern Humans. The Early Upper Palaeolithic in Europe — dated to the second half of the Interpleniglacial — was, at least partially, based on these “transitional” industries and manifested by the appearance of the Aurignacian, contrasted with local cultures such as the Uluzzian in Mediterranean Europe. During whole the Interpleniglacial Europe was separated from Northern Africa dominated by local evolution of Middle Palaeolithic (Middle Stone Age) cultures (mostly expressed by the Aterian), and by specific “transitional” industries on the southern Mediterranean coast (Early Dabbian) and in the Lower Nile basin. The Last Glacial Maximum and the corresponding sea level recession opened new possibilities of contacts between the Maghreb and the Iberian Peninsula in both directions (Aterian-Solutrean and Gravettian-Early Iberomaurusian), which are still difficult to be proved before new chronostratigraphic correlations are made. At the same time we register links between south-eastern Europe and western Anatolia; the real border between Near Eastern and European Mediterranean cultural zones was marked, in the Late Glacial, by the Taurus chain. During the Late Glacial the cultural separation between Europe and Africa was particularly marked. Only in the Aegean basin the first sea navigation facilitated contacts which become widespread as late as in the Early Holocene with neolithization trough maritime contacts.     

Origins of the Iberomaurusian in NW Africa: New AMS radiocarbon dating of the Middle and Later Stone Age deposits at Taforalt Cave,Morocco

Recent genetic studies based on the distribution of mtDNA of haplogroup U6 have led to subtly different theories regarding the arrival of modern human populations in North Africa. One proposes that groups of the proto-U6 lineage spread from the Near East to North Africa around 40–45 ka (thousands of years ago), followed by some degree of regional continuity. Another envisages a westward human migration from the Near East, followed by further demographic expansion at ∼22 ka centred on the Maghreb and associated with a microlithic bladelet culture known as the Iberomaurusian. In evaluating these theories, we report on the results of new work on the Middle (MSA) and Later Stone (LSA) Age deposits at Taforalt Cave in Morocco. We present 54 AMS radiocarbon dates on bone and charcoals from a sequence of late MSA and LSA occupation levels of the cave. Using Bayesian modelling we show that an MSA non-Levallois flake industry was present until ∼24.5 ka Cal BP (calibrated years before present), followed by a gap in occupation and the subsequent appearance of an LSA Iberomaurusian industry from at least 21,160 Cal BP. The new dating offers fresh light on theories of continuity versus replacement of populations as presented by the genetic evidence. We examine the implications of these data for interpreting the first appearance of the LSA in the Maghreb and providing comparisons with other dated early blade and bladelet industries in North Africa.     

Origin and diffusion of mtDNA haplogroup X

A maximum parsimony tree of 21 complete mitochondrial DNA (mtDNA) sequences belonging to haplogroup X and the survey of the haplogroup-associated polymorphisms in 13,589 mtDNAs from Eurasia and Africa revealed that haplogroup X is subdivided into two major branches, here defined as “X1” and “X2.” The first is restricted to the populations of North and East Africa and the Near East, whereas X2 encompasses all X mtDNAs from Europe, western and Central Asia, Siberia, and the great majority of the Near East, as well as some North African samples. Subhaplogroup X1 diversity indicates an early coalescence time, whereas X2 has apparently undergone a more recent population expansion in Eurasia, most likely around or after the last glacial maximum. It is notable that X2 includes the two complete Native American X sequences that constitute the distinctive X2a clade, a clade that lacks close relatives in the entire Old World, including Siberia. The position of X2a in the phylogenetic tree suggests an early split from the other X2 clades, likely at the very beginning of their expansion and spread from the Near East.     

The Ecology of Seasonal Stress and the Origins of Agriculture in the Near East

The time, place, and reasons for the first domestication of cereals and legumes in the Near East can now be securely identified using combined evidence from paleoenvironmental studies, models of ecosystem dynamics, and regional archeology. The heartland of domestication was the Jordan Valley and surrounding region in the Southern Levant. Approximately 10,000 years ago, people began planting crops where the wild ancestral species had proliferated over two millenia. Impetus for domestication came from the synergistic effects of climatic change, anthropogenic environmental change, technological change, and social innovation. At the end of the Pleistocene, after a long period of climatic instability, a mediterranean climate more strongly seasonal than any today emerged with hyper-arid summers that selected for annual species of cereals and legumes. This occurred long after people had invented tools suitable for grinding hard seeds, but the new, lengthy dry season and consequent need to use stored foods encouraged sedentism among human groups who subsequently depleted their immediate environments of wild resources. These preconditions facilitated the development of agriculture. The scenario developed here is specific to the Near East, for such case studies of specific factors in independent regions of domestication are essential before we attempt to explain cases all over the world with reference to global causes.     

Palaeogenetic evidence supports a dual model of Neolithic spreading into Europe

The peopling of Europe is a complex process. One of the most dramatic demographic events, the Neolithic agricultural revolution, took place in the Near East roughly 10000 years ago and then spread through the European continent. Nevertheless, the nature of this process (either cultural or demographic) is still a matter of debate among scientists. We have retrieved HVRI mitochondrial DNA sequences from 11 Neolithic remains from Granollers (Catalonia, northeast Spain) dated to 5500 years BP. We followed the proposed authenticity criteria, and we were also able, for the first time, to track down the pre-laboratory-derived contaminant sequences and consequently eliminate them from the generated cloning dataset. Phylogeographic analysis shows that the haplogroup composition of the Neolithic population is very similar to that found in modern populations from the Iberian Peninsula, suggesting a long-time genetic continuity, at least since Neolithic times. This result contrasts with that recently found in a Neolithic population from Central Europe and, therefore, raises new questions on the heterogeneity of the Neolithic dispersals into Europe. We propose here a dual model of Neolithic spread: acculturation in Central Europe and demic diffusion in southern Europe.     

A microsatellite survey of cattle from a centre of origin: the Near East

Eight humpless cattle breeds from the Near East, three from Europe, one from West Africa and two zebu breeds from India were screened with 20 microsatellite loci. Breeds from the Near East revealed considerable levels of introgression from zebu cattle, which was apparent most in populations from the East and which declined in populations further West. This nonrandom pattern is suggestive of the introduction of zebu cattle from the East. Notwithstanding the overlay of zebu alleles, it was possible to demonstrate that Near Eastern cattle exhibited significantly higher levels of allelic diversity than breeds from other regions, which is consistent with the view that this region represents a primary domestication centre for Bos taurus cattle. The hypothesis that B. taurus and B. indicus cattle have separate domestic origins is also supported by the survey, a large genetic divergence being apparent between the nonhybrid taurine and zebu groups.     

Molecular phylogeography of domesticated barley traces expansion of agriculture in the Old World

Barley (Hordeum vulgare ssp. vulgare) was first cultivated 10,500 years ago in the Fertile Crescent and is one of the founder crops of Eurasian agriculture. Phylogeographic analysis of five nuclear loci and morphological assessment of two traits in >250 domesticated barley accessions reveal that landraces found in South and East Asia are genetically distinct from those in Europe and North Africa. A Bayesian population structure assessment method indicates that barley accessions are subdivided into six clusters and that barley landraces from 10 different geographical regions of Eurasia and North Africa show distinct patterns of distribution across these clusters. Using haplotype frequency data, it appears that the Europe/North Africa landraces are most similar to the Near East population (F ST = 0.15) as well as to wild barley (F ST = 0.11) and are strongly differentiated from all other Asian populations (F ST = 0.34-0.74). A neighbor-joining analysis using these F ST estimates also supports a division between European, North African, and Near East barley types from more easterly Asian accessions. There is also differentiation in the presence of a naked caryopsis and spikelet row number between eastern and western barley accessions. The data support the differential migration of barley from two domestication events that led to the origin of barley--one in the Fertile Crescent and another farther east, possibly at the eastern edge of the Iranian Plateau--with European and North African barley largely originating from the former and much of Asian barley arising from the latter. This suggests that cultural diffusion or independent innovation is responsible for the expansion of agriculture to areas of South and East Asia during the Neolithic revolution.     

Searching for the origins of arable weeds in the Near East

This short note adds to earlier attempts at identifying arable weeds on late Pleistocene/early Holocene sites in the Near East. Nineteen potential arable weed taxa that have no known use were selected. The occurrence of these taxa at sites with morphologically wild cereals was compared to sites with morphologically domestic cereals. The presumed arable weed taxa were as common on three PPNA (Pre Pottery Neolithic A) sites without domestication as they were on Middle PPNB (Pre Pottery Neolithic B) sites with domestication, which lends support to arguments for pre-domestic cultivation at the former sites. Arable weed taxa were less common at Natufian sites but their presence raises the question of whether they originated in cultivated fields or were the ancestors of weeds gathered accidentally alongside wild cereals in their natural habitat.