美丽花网翅虻Florinemestrius pulcherrimus Ren

本期封面化石照片为美丽花网翅虻Florinemestrius pulcherrimusRen,1998,隶属于双翅目短角亚目网翅虻科,采自中国辽宁省北票市晚侏罗世义县组地层。虫体保存十分完整,不仅翅脉、足、腹部清晰可见,口器以及虫体的细毛均保存良好。在辽西义县地层中还有大量该科的化石,分别归于2属3种。照片中的F.pulcherrimus拥有较短的长喙,另一种Protonemestrius jurassicus的喙近体长一半。现生的网翅虻是主要的访花昆虫,一些种类以花蜜和花汁为食。综合分析这批化石虻类的长喙、体毛以及与现生类群的比较,作者认为这些生活在晚侏罗纪的喜花虻类很可能利…     

河北和辽宁晚侏罗世古蝉化石(同翅目:头喙亚目)(英文)

本文描述古蝉科化石2新属、6新种,即Liaocossushuegen.etsp.nov.,L.beipiaoensisgen.etsp．nov,L．exiguusgen．etsp．nov,L．fengningensisgen．etsp．nov,L．Pingquanensisgen.etsp.nov.,Miracossusingentiusgen．etsp．nov.。这批化石保存有完整的虫体标本,使作者能够进一步研究古蝉类化石的虫体特征。化石采于冀北和辽西晚侏罗世义县组地层中,保存在中国地质博物馆。     

中国东北晚侏罗世蝎蛉化石研究(长翅目:蚊蝎蛉科,直脉蝎蛉科)(英文)

本文描述蝎龄化石1新属4新种。新属Megabittacus2新种,即Megabittacuscolosseussp.nov.,Megabittacusbeipiaoensissp．nov,新种Sibirobittacusatalussp.nov.属于现生的蚊蝎蛉科,新种Orthophlebialiaoningensissp．nov．属于绝灭的直脉蝎蛉科。化石采于辽宁省北票市晚侏罗世义县组地层中。这些新的化石属种,其虫体特征包括触角、喙、足和翅脉特征均完整的保存。模式标本保存在中国地质博物馆。     

中国麻虻属新种和新记录种（双翅目：虻科）

本文记述采自云南省的虻科一新种亚沥青麻虻Hoematopta subpicea和一新记录种特拉斯里麻虻Haematopota tenasserimi。新种亚沥青麻虻一对翅的r_4附脉处均具小翅室,并成严格对称,该特征在虻科分类中首次发现,为探讨双翅目昆虫翅脉的演化及其翅室的形成提供了一个重要线索。模式标本保存于中国科学院上海昆虫研究所。     

我国侏罗纪脉翅目化石一新属一新种(脉翅目:蚁蛉总科)

描述脉翅目化石一新属一新种,精细华蛉Siniphes delicatus gen.et sp.nov.,标本采自辽宁省北票市晚侏罗世义县组地层中。讨论了华蛉属的科级分类问题。新属可归于蚁蛉总科,但科级位置不能确定。模式标本保存在首都师范大学生物系。     

中国内蒙古中侏罗世脉翅目昆虫化石一新科

建立脉翅目昆虫化石新科-Grammolingiidae fam nov,并描述该科化石3新属6新种;Grammolingia boigen.nov.of sp.nov,Litholingia rhora gen.nov.of sp.nov,Litholingia eumorpha gen.nov.of sp.nov,Litholing-sp.nov给出了新科的属级检索表,描述的有模式标本均采自于内蒙古自治区宁城县中侏罗世九龙山组地层中,标本分别保存在义县博物馆和首都师范大学生物系。本次发现再次表明目前关于脉翅目昆虫的认识仍然十分贫乏。     

辽宁凌源及内蒙古宁城地区下白垩统义县组脊椎动物生物地层

辽宁凌源及相邻内蒙古南部宁城地区义县组发现多个重要脊椎动物化石地点,通过岩 石地层、脊椎动物生物地层和同位素年代地层对比,建立了该地区义县组地层层序。凌源一宁 城地区义县组有 ２个脊椎动物化石层位：下部道虎沟层含 Ｌｙｃｏｐｔｅｒａｅｒ Ｉｃｈｔｈｙｏｆａｕｎａ, Ｐｓｉｔｔａｃｏｓａｕｒｕｓ Ｆａｕｎａ和Ｃｏｎｆｕｃｉｕｓｏｒｎｉｓ Ａｖｉｆａｕｎａ等３个动物群,可以与北票尖山沟层的四合 屯脊椎动物化石群对比,层位相当于义县组一、三段;上部大王杖子（大新房子）层含 Ｌｙｃｏｐｔｅｒａ Ｉｃｈｔｈｙｏｆａｕｎａ和Ｌｉａｏｘｉｏｒｎｉｓ Ａｖｉｆａｕｎａ等２个动物群,为义县组中部沉积,在四合屯地 区缺失。冀北丰宁四岔口－森吉图地区义县组脊椎动物化石层对应于凌源大王杖子层;滦平 大北沟组为义县组最下部沉积,其上覆的大店子组相当于宁城道虎沟层和北票四合屯义县组 一、三段。热河群义县组和九佛堂组发现３个连续的鸟类群;义县组下部Ｃｏｎｆｕｃｉｕｓｏｒｎｉｓ Ａｖｉｆａｕｎａ、义县组中部Ｌｉａｏｘｉｏｒｎｉｓ Ａｖｉｆａｕｎａ和九佛堂组Ｃａｔｈａｙｏｒｎｉｓ－Ｃｈａｏｙａｎｇｉａ Ａｖｉｆａｕｎａ。义 县组和九佛堂组Ｌｙｃｏｐｔｅｒａ　Ｉ     

中国内蒙古中侏罗世脉翅目昆虫化石—新科(英文)

建立脉翅目昆虫化石新科———Grammolingiidaefamnov .,并描述该科化石 3新属 6新种 :Grammolingiaboigen .nov .ofsp .nov .,Litholingiarhoragen .nov .ofsp .nov .,Litholingiaeumorphagen .nov .ofsp .nov .,Litholing iapolychotomagen .nov .ofsp .nov .,Leptolingiajurassicagen .nov .ofsp .nov .,Leptolingiatianyiensisgen .nov .etsp .nov ..给出了新科的属级检索表 ,描述的所有模式标本均采自于内蒙古自治区宁城县中侏罗世九龙山组地层中。标本分别保存在义县博物馆和首都师范大学生物系。本次发现再次表明目前关于脉翅目昆虫的认识仍然十分贫乏。     

中国斑翅剑虻属二新种（双翅目：剑虻科：剑虻亚科）

记述中国斑翅剑虻属Hoplosathe 2新种:盛氏斑翅剑虻Hoplosathe shengi sp. nov.和吐鲁番斑翅剑虻Hoplosathe turpanensis sp. nov.。盛氏斑翅剑虻的额在斑翅剑虻属中最宽,雄虫和雌虫的额宽在触角水平上分别是头宽的42%和 46%;触角第1鞭节长是宽的2.0倍;雄性生殖器近似科氏斑翅剑虻Hoplosathe kozlovi Lyneborg & Zaitzev.吐鲁番斑翅剑虻体多毛或短鬃;额宽正常;触角第1鞭节长是宽的2.8倍;雄性生殖器与斑翅剑虻属其它种皆有很大区别。     

珍贵的小龙虾蜕皮化石

淡水小龙虾化石在世界上十分少见。龙虾蜕皮的化石还未曾有过记录。文中讨论热生物群上侏罗统义县组发现的,保存十分完好的蜕皮化石。判断其蜕皮标本的３个理由是：没有保存头胸甲;步足与体节之间的关节膜很薄以及几乎看不到磷盐化的软组织。     

dbEST-derived SSR markers in sea urchin (Hemicentrotus pulcherrimus)

Sea urchin is an important model organism for evolutionary biology, embryology, and gene regulation study. We developed and evaluated simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) of Strongylocentrotus purpuratus and Hemicentrotus pulcherrimus. Characteristics of nine EST-SSR loci were investigated using 41 Hemicentrotus pulcherrimus individuals. The number of alleles per locus ranged from two to five. The observed heterozygosity ranged from 0.122 to 0.7805, while the expected heterozygosity ranged from 0.4472 to 0.7696. These loci and markers will be useful for population genetics and systemic evolution among species of sea urchin.     

Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos

The Hemicentrotus pulcherrimus homologue of nanos (HpNanos), that encodes a protein containing two CCHC zinc finger motifs, was isolated from a gastrula cDNA library. The accumulation of HpNanos mRNA during embryonic development and the spatial expression pattern are reported. Developmental northern blot analysis revealed that HpNanos mRNA markedly accumulated during the blastula stages, and then decreased in abundance at the mesenchyme blastula stage. The second phase of HpNanos mRNA expression occurred during gastrulation, after which the expression returned to a low level. Whole-mount in situ hybridization showed that the HpNanos was exclusively expressed in four to six small micromere-descendant cells at the blastula stage. The expression of HpNanos was restricted to the coelomic pouch, which gives rise to the mesoderm of the ventral surface of the adult rudiment, at the prism stage. These results suggest that HpNanos expression will be instrumental for future analyses of the function of small micromere-descendant cells and of the origin of germ cells during sea urchin development.     

The Participation of Speract in the Acrosome Reaction of Hemicentrotus pulcherrimus

A speract-free macromolecular fraction was prepared from the egg jelly of the sea urchin Hemicentrotus pulcherrimus by gel filtration and tested for ability to induce the acrosome reaction in H. pulcherrimus spermatozoa with or without exogenously added synthetic speract. The macromolecular fraction without speract showed only about half the activity of the original unfractionated jelly for induction of the acrosome reaction. The rates of the acrosome reaction induced by the fraction with speract were comparable with those induced by the unfractionated jelly at all pHs tested. Speract itself, however, did not induce the acrosome reaction in the absence of the macromolecular fraction of jelly. The acrosome reaction was associated with incerase of the cyclic AMP concentration in sperm cells, the extent of incerase depending on the concentration of the macromolecular fraction. Addition of speract to the fraction enhanced both induction of the acrosome reaction and increase in the cyclic AMP concentration induced by the fraction. These results suggest that a major factor(s) responsible for the acrosome reaction is a macromolecular component(s) of the jelly and that speract promotes the reaction as a co-factor.     

Centriole behavior during meiosis in oocytes of the sea urchin Hemicentrotus pulcherrimus

Ultrastructural changes in the maturing oocyte of the sea urchin Hemicentrotus pulcherrimus were observed, with special reference to the behavior of centrioles and chromosomes, using oocytes that had spontaneously started the maturation division process in vitro after dissection from ovaries. The proportion of oocytes entering the maturation process differed from batch to batch. In those eggs that accomplished the maturation division, it took ~4.5-5 h from the beginning of germinal vesicle breakdown to the formation of a second polar body. Serial sections revealed that a young oocyte before germinal vesicle breakdown had a pair of centrioles with procentrioles, located between the presumed animal pole and the germinal vesicle and accompanied by amorphous aggregates of moderately dense material and dense granules (granular aggregate). Just before germinal vesicle breakdown, a pair of fully grown centrioles located in the granular aggregate, which is present until this stage and then disappears, had already separated from another pair of centrioles. In meiosis I, each division pole had two centrioles, whereas in meiosis II each had only one. The two centrioles in the secondary oocyte separated into single units and formed the mitotic figure of meiosis II. The first polar body had two centrioles and the second had only one. The two centrioles in the first polar body did not form the mitotic figure nor did they separate at the time of meiosis II. These results indicate that, in sea urchins, duplication of the centrioles does not occur during the two successive meiotic divisions and the egg inherits only one centriole from the primary oocyte, confirming the results previously reported for starfish oocytes.     

Behavior of pigment cells in gastrula-stage embryos of Hemicentrotus pulcherrimus and Scaphechinus mirabilis

The behavior of pigment cells in sea urchin embryos, especially at the gastrula stage, is not well understood, due to the lack of an appropriate method to detect pigment cells. We found that pigment cells emanated autofluorescence when they were fixed with formalin and irradiated with ultraviolet or green light. In Hemicentrotus pulcherrimus, fluorescent pigment cells became visible at the archenteron tip at the mid-gastrula stage. The cells detached from the archenteron slightly before the initiation of secondary invagination and migrated toward the apical plate. Most pigment cells entered the apical plate. This entry site seemed to be restricted, because pigment cells could not enter the ectoderm and remained in the blastocoele at the vegetal pole side when elongation of archenteron was blocked. Pigment cells that had entered the apical plate soon began to migrate in the aboral ectoderm toward the vegetal pole. In contrast, pigment cells of Scaphechinus mirabilis embryos were first detected in the vegetal plate before the onset of gastrulation. Without entering the blastocoele, these cells began to migrate preferentially in the aboral ectoderm toward the animal pole. When the archenteron tip reached the apical plate, pigment cells had already distributed throughout the aboral ectoderm. Thus, the behavior of pigment cells was quite different between H. pulcherrimus and S. mirabilis.     

Salegentibacter echinorum sp. nov., isolated from the sea urchin Hemicentrotus pulcherrimus

A novel Gram-negative, strictly aerobic, heterotrophic, non-motile and yellow-pigmented bacterial strain, designated HD4^T, was isolated from the sea urchin Hemicentrotus pulcherrimus collected from the Yellow Sea in China. Optimal growth of the strain was observed at 28–30 °C, pH 6.8–7.3, and in the presence of 3–5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain HD4^T exhibited high similarity with the members of Salegentibacter (92.3–95.4 %). The DNA G+C content was 37.0 mol%, MK-6 was the main respiratory quinone and summed feature 3 (comprising iso-C_15:0 2-OH/C_16:1ω7c), iso-C_15:0, iso-C_17:0 3-OH and anteiso-C_15:0 were the major cellular fatty acids. The predominant polar lipids in strain HD4^T were phosphatidylethanolamine and two unknown lipids (L2, L4). Based on the phylogenetic, physiological and biochemical characteristics, strain HD4^T should be classified as a novel species within the genus Salegentibacter, for which the name Salegentibacter echinorum sp. nov. is proposed. The type strain is HD4^T (=CICC 10466^T = NRRL B-59666^T).     

Vitelline layer lytic activity in sperm extracts of sea urchin,Hemicentrotus pulcherrimus

A factor which dissolves the vitelline layer was extracted from sperm of the sea urchin, Hemicentrotus pulcherrimus. Turbidity of the suspension was reduced when isolated vitelline layers were mixed with this sperm factor. When the mixture was subjected to SDS polyacrylamide gel electrophoresis, some of the protein bands of the vitelline layer were seen to be missing. The lytic activity of the factor was heat labile, completely inhibited by L-1-tosyl-amide-2-phenyl-ethylchloromethyl ketone and partially inhibited by soybean trypsin inhibitor. Chymotrypsin activity was detected, but not trypsin, arylsulfatase, or glycosidase. These results suggest that a chymotrypsin-like enzyme participates in lysis of the vitelline layer by the fertilizing spermatozoon.     

Development of expressed sequence tag-derived microsatellite markers for the sea urchin Hemicentrotus pulcherrimus

The first set of 15 expressed sequence tag-simple sequence repeat markers was developed in sea urchin Hemicentrotus pulcherrimus. Number of alleles per locus ranged from two to 17. The observed and expected heterozygosities ranged from 0.022 to 0.911 and from 0.022 to 0.916, respectively. These informative marker loci will be useful for the assessment of genetic variation and population structure of this species.